805, Lefort regia

"Interdisciplinary team?" Lu Weiwei was stunned, and couldn't help but say: "It feels so powerful to hear the name, is there not only forensic medicine, but also other police and personnel from other professions involved?"

Forensic Doctor Liu nodded slightly: "That's pretty much what it means, because we in Jiangnan City, we still need to strengthen training in diatom detection, otherwise Gao Chuanfeng wouldn't have asked such a stupid question." ”

"Ugh. Suddenly feeling like he was being shot, Gao Chuanfeng felt that the whole person was not good.

Gu Chen frowned slightly, and said tentatively: "To form such an interdisciplinary team, it is necessary to draw backbone forces, and the team members must have forensics, materials, chemistry, and biology." ”

"Yes, you're absolutely right. Forensic Doctor Liu completely agreed with Gu Chen's opinion, and couldn't help but say:

"With this interdisciplinary team, the next step is to focus on three problems. ”

Officer Wang didn't understand very well, so he asked for advice: "Forensic Doctor Liu, can you be more specific? What are the three problems?" ”

After completing the phased testing on his hands, Forensic Doctor Liu turned around and said, "The first thing is to dissolve the tissue and retain the shell of the diatoms. ”

"Secondly, diatoms need to be enriched, and the third is process pollution prevention. ”

Seeing that everyone looked down and thought, Forensic Doctor Liu said: "These seemingly simple steps, in fact, if they are not operated properly in the process, there will be many problems. ”

For example, for diatom detection technology, this kind of research is not new, as early as 2003, the team purchased an electron microscope for the study of alien particles in drowned corpses. ”

They used foreign bodies in corpses to determine the cause and location of death, and during the experiment, the forensic doctors were pleasantly surprised to find that the practical application of diatoms was better, but also flawed. ”

"Master, don't talk about the advantages, let's talk about the disadvantages. Forensic assistant Kaede Takakawa is also a little impatient.

Forensic Doctor Liu glanced at him and said, "If it's a shortcoming, it's an operational defect." ”

"Operation defects?" Gao Chuan Feng was stunned, and quickly frowned and asked, "What exactly does it mean?"

"In practice, the technical effect of diatom testing may not be satisfactory. Gu Chen answered.

Forensic Doctor Liu nodded slightly: "That's right, the operation defect I said refers to this, because the effect is relative." ”

Lu Weiwei thought about it, and also asked Forensic Doctor Liu: "Why is it and what is the reason?"

"This ......" Forensic Doctor Liu just wanted to speak, but he glanced at Gu Chen beside him, so he asked Gu Chen directly: "This question, Gu Chen, what do you think?"

"I think it's a pollution problem. Gu Chen answered directly: "Pollution is a big problem, and the detection rate is low, and the third is that the standard cannot be formed." ”

Forensic Doctor Liu smiled slightly: "Gu Chen, you go on." ”

"Yes. Gu Chen nodded silently, and only after a few seconds of silence did he explain to everyone:

"Maybe for this kind of test, a famous master can do it, but he can do it, others can't, that is to say, there is no repeatability. ”

"But if there is no repetition, it can only be called experience, not science. ”

"That's great. Forensic Doctor Liu very much agreed with Gu Chen's opinion, and couldn't help but say: "Take myself as an example, maybe for testing, with my own rich testing experience, I may be able to succeed." ”

"But if, I mean if, leave it to Kaede Takakawa, and there are other assistants to do it. ”

"In terms of their experience, they are definitely not as good as mine, and the consequence of their lack of experience is that the detection is not ideal and the accurate effect is not achieved. ”

So this is what Gu Chen said, without repetitive things, it can only be called experience, not science. ”

"But to solve this problem, in fact, we have taken a lot of detours. ”

"Yes. Gu Chen also felt the same way, nodded slightly, and said, "For many years, in fact, the most traditional way to find diatoms is ...... It is a tissue specimen that is taken from a cadaver. ”

"After extraction, the tissue is digested with mixed acids, followed by a series of procedures such as centrifugation and separation, and finally extracted into glass slides. ”

"But after that, we have to use an optical microscope to look for diatoms. ”

After a pause, Gu Chen said again: "But there are many problems with such a traditional extraction method. ”

"Because the traditional strong acid digestion method is open, the digestion process is ...... There is a danger of burning the human body with strong acid sprays. ”

"Moreover, the nitrogen dioxide and odorous gases released by strong acids and samples during the digestion process are easy to pollute the environment. ”

"There are so many troubles?" Officer Wang couldn't understand what Gu Chen was saying at all, after all, these were beyond his cognitive outline.

Gu Chen nodded slightly, and continued: "That's right, it's quite troublesome, and this is also a disadvantage. ”

In addition, traditional digestion generally needs to be repeated for multiple centrifugation and then prepared for observation, and about 30% of the diatoms will be lost after one centrifugation, resulting in low recovery rate of diatom extraction. ”

"In addition, traditional optical microscopes have limited magnification, rely on manual search and analysis, and for microdiatoms, it is easy to miss detection, or it is impossible to accurately identify their species. ”

"However, the characteristics of diatoms determine that it is still the 'gold standard' for drowning diagnosis, and through the analysis of diatoms, not only can we have a more direct judgment on the cause of death, but also point to the possible place of drowning. ”

"Yes, what Gu Chen said is very good. Forensic Doctor Liu, who has completed the next step of testing, is carrying out various operations in his hands, and his back is facing everyone:

"It is precisely because of the traditional way of testing...... There are great drawbacks, so overcoming difficulties and improving technology can highlight the results. ”

"In this regard, the peers in Yangcheng have done a really good job. ”

"So, how can the existing diatom testing methods be upgraded to make them practical?"

"The colleagues in Yangcheng have formed an 'interdisciplinary team' composed of technical backbones in the criminal department, and the team members are not only forensic medicine, but also materials science, chemistry and biology. ”

"It is precisely because of such an interdisciplinary team with multiple backbone talents that we can make breakthroughs in dissolving tissues, retaining the shell of diatoms, enriching diatoms, and preventing pollution in the process. ”

Looking back at everyone, Forensic Doctor Liu took a deep breath and said with a light smile: "In order to do experimental research, people often arrange experiments at night and in the field, just like us. ”

"Because we do research on drowning, we can't attract too much attention from society, let alone affect everyone's daily life. ”

"Sometimes they go to the lake in the middle of the night to do research, as far as the sea, and they also call in sanitation trucks to transport the lake water back to the laboratory for research. ”

It is precisely because of this spirit of overcoming difficulties that people have gone through technological exploration one by one, from traditional strong acid digestion to centrifugal enrichment, to light microscopy inspection method, to microwave digestion, to filter enrichment, to scanning electron microscopy. ”

"In this way, the time for processing diatoms can be ...... It has been shortened by a full three-quarters, and the positive rate of diatoms has also increased from 28% to 97%, which is a qualitative leap. ”

"That's right. Gu Chen smiled slightly, and continued to add Forensic Doctor Liu's words: "In fact, the use of microwave digestion method instead of traditional strong acid digestion shortens the processing time, reduces the pollution to the environment, and the speed is fast, and the digestion effect is thorough." ”

"The use of membrane filtration not only replaces the traditional centrifugal treatment, but also greatly improves the recovery rate of diatom extraction. ”

"And the diameter of the filter pore on this membrane is only 0.45 microns. ”

Glancing at everyone, Gu Chen deliberately reminded: "Please note that it is only 0.45 microns, which is enough to stop diatoms in nature. ”

"And the use of electron microscopes, instead of traditional optical microscopes. ”

Lu Weiwei nodded silently when she heard this, and asked Gu Chen: "I know that the detection effect of electron microscope is much higher than that of traditional optical microscopes. ”

"But Senior Brother Gu, can you give a specific comparison so that we can have a more intuitive understanding. ”

"Ahem. In the face of Lu Weiwei's curiosity, Gu Chen coughed dryly twice, and also adjusted his tone:

"Then I'll tell you this, the magnification of traditional light mirror detection is only about 400 times, and it also needs to be recognized by the human eye. ”

"Electron microscopes, on the other hand, can perform automatic scanning, which is fast, accurate, and easy to automate. ”

"It's just technology, and the two are not on the same level. ”

"So that's the case, wouldn't that be simpler and more accurate?" Lu Weiwei said with her right hand and finger with her left hand: "Automatic scanning, fast and accurate, easy to automate, then any person can get started." ”

"That's right. Forensic Doctor Liu smiled at Lu Weiwei and said: "It's a coincidence, we have one in our laboratory, so it's not complicated to detect, the key is that our peers in Yangcheng have paved the way for us." ”

"Yes. Gu Chen walked to an electron microscope and couldn't help but say:

"I remember that the scientific research team in Yangcheng has achieved remarkable results in a series of research on scientific instruments and scientific methods. ”

Moreover, they have also published many papers in top international forensic science journals, and have been invited to participate in the International Forensic Science Conference for many years to give academic reports. ”

In addition, they have also obtained 4 invention patents and 3 utility model patents. ”

"Hey, hey. When Forensic Doctor Liu heard this, he was also envious and said: "They are mainly a professional team composed of backbone forces. ”

"In fact, I also set up a research project many years ago, but at that time, I was not very qualified and sophisticated, so it was impossible to find this backbone interdisciplinary team member. ”

"And even if I have interdisciplinary team members, and each of them is the backbone, it will be difficult to make a difference in this project if the research funding is not in place. ”

As if with regret, Forensic Doctor Liu also took a deep breath after listening to Gu Chen's praise of his peers in Yangcheng, and tried to calm down.

Everyone seemed to feel the helplessness of Forensic Doctor Liu, so they could only look at each other and didn't dare to make a sound.

Forensic Doctor Liu glanced at the people behind him, and suddenly sneered at himself and said with a smile: "In fact, to complete such a major project, it is not only the investment of talents, but also the investment of funds, software and hardware equipment. ”

"And even if you have a professional interdisciplinary team, it will take years to do iterative research, and it may not always be fruitful. ”

"Therefore, to obtain an upgraded inspection plan, it is not only necessary to look at the ability of the team, but also to see the determination of the top, to see whether they are willing to invest high and tilt resources to scientific research projects. ”

"Scientific research projects are often the most expensive, but they are not as fast as real estate buying and selling, and they cannot produce results quickly. ”

"If the supervisor is patient, he is afraid that the leader will be replaced, and the follow-up of the project will be cut for other expenses. ”

"Anyway, it's really not that simple to set up a scientific research project. ”

"Although I am no longer a rookie in the past, I have also made suggestions from the leaders above me and have been able to obtain a lot of scientific research funds. ”

"But technology is always evolving, and if you don't do it in the first place, people who are more willing to do it will be the first to do it. ”

"So now they have done these things, and they have also upgraded the testing program and widely promoted the new technology. ”

"The testing solution we are using now is based on the results of others. ”

"Forensic Doctor Liu, don't be discouraged. Seeing that Forensic Doctor Liu was a little emotional, Lu Weiwei hurriedly came over and comforted:

"Even if the diatom testing project is the first to produce results, we can still make breakthroughs in other fields. ”

"Huh. Seeing that Lu Weiwei was so naΓ―ve, forensic assistant Gao Chuanfeng couldn't help laughing dryly, and said in a mocking tone:

"I said Lu Weiwei, do you think that scientific research projects are so easy to operate? Let me tell you, over the years, these so-called elite colleagues have not been idle. ”

"All scientific research projects that are easy to operate and easy to produce results have basically been done by others, and it is even more difficult to find some scientific research projects in emerging fields at this stage. ”

"There aren't a few decent high-standard labs to build on, so you're going to show me?"

Lu Weiwei was stunned for a moment, and said weakly: "Okay, okay, I'll just talk about it casually, I'm really not good at scientific research, but Senior Brother Gu can help." ”

When Gu Chen heard this, he also nodded silently: "That's right, if there is anything that can be used for me Gu Chen, I am still willing to help without affecting my work, after all, I also need to learn." ”

"Haha. Hearing Gu Chen say that he also wanted to study, Forensic Doctor Liu laughed on the spot and said: "It's just that you Gu Chen have this enthusiasm, and it is not in vain that the technical department of our Municipal Bureau will give you priority to do testing every time." ”

Looking at the electron microscope in front of him, Forensic Doctor Liu also sighed: "There are new technologies, but at the beginning, the cost of the required equipment was too high, such as microwave and scanning electron microscope, and not every public security unit, especially the grassroots units, could afford it." ”

"So, why are our peers in Yangcheng so good, because they also helped us think of it. ”

To this end, they have also considered high-quality and low-cost universal access programs. ”

"What do you mean...... Lefort Wang Shui?" asked Gu Chen.

Forensic Doctor Liu nodded fiercely: "Yes, it's Lefort Wang Shui, I can't imagine that you Gu Chen even know this?"

"I understand a little bit, but I only understand the surface. Gu Chen actually knows more about this technology, and has even made special reading notes in some scientific research magazines.

But Gu Chen would not be too high-minded, but only modestly said that he knew some fur, and returned the interpretation of Lefort Wang Shui to Forensic Doctor Liu.

Forensic Doctor Liu also nodded and couldn't help but say: "Because of the high cost of the microwave digestion instrument, Lefort aqua regia was invented, that is, a mixture of three parts of nitric acid and one part of hydrochloric acid. ”

"The effect is better than the digestion of traditional strong acids, with the characteristics of simple, efficient, qualitative and quantitative analysis, low cost, simple operation, and strong practicability. ”

Looking at the electron microscope in front of him, Forensic Doctor Liu smiled again: "The cost of electron microscope is also very high, so our colleagues in Yangcheng will find a way to make the filter membrane transparent, so that it can be observed through the optical microscope." ”

In order to fill the gap in the research of diatoms, they also collected water samples from all over the country, observed and classified the components of diatoms, and systematized the research of diatoms in China. ”

"In this regard, people are really hardworking, and it is natural to get such a great honor, and we don't need to be jealous. ”

"That's right, no pain, no gain. Lu Weiwei also said casually, I just feel that this kind of scientific research is too cumbersome, and it is not something that ordinary scientific research teams can operate.

Forensic Doctor Liu said with a smile: "The multi-vacuum diatom enrichment equipment and consumables developed by the Yangcheng Criminal Department have achieved industrial transformation and have been listed by the Ministry of Public Security as a necessary equipment for provincial public security organs. ”

"At present, the criminal department of Yangcheng has held many training courses, attracted counterparts from all over the country to participate in the study, and assisted many provinces and cities to establish diatom testing laboratories. ”

"Not only that, but it has also tested a series of difficult cases for the whole country, and all of them have reached clear conclusions, which have played a key role in the characterization, disposal and litigation of the cases. ”

"And the most important thing is ...... Whether it's to assist with inspections or to come to learn, they don't take a penny. ”

"Don't take a penny?" When he heard this, Officer Wang was also shocked: "So...... That's the pattern. ”

"Not really. Forensic Doctor Liu also smiled and said: "People can solve problems for everyone, that is the happiest thing for people." ”

"Moreover, after the development of this technology, the petition cases of corpses in water in Guangdong Province have increased from a dozen to a few cases per year in the past, and there have been no petition cases in the past three years, which is also the social benefit of the new method of forensic diatom testing. ”

"Recently, they are going to hold a training course in Yangcheng to help train inspectors from all over the country to be proficient in the latest diatom testing technology. ”

"I'm also going to pick a team to participate, I don't know...... Are you interested?"

......