"Of course, Zheng, that's a lease agreement signed between Celia and MIT's logistics department, as long as you pay the rent on time and don't do illegal research, of course you can continue to rent——"

Aurora's voice came without hesitation, Zheng Jianguo breathed a sigh of relief, he was not interested in continuing research in Thomas Laboratory, this is not to say that he does not know that this field is the hottest hot spot in the world, but because with the development of this technology in his memory, there will be absolutely no progress in at least thirty years.

Therefore, the Massachusetts Institute of Technology terminated the previous employment agreement, and Zheng Jianguo only needed to remove the title of associate professor on the business card, and by the way, he got back his due weekends and weekends.

In addition, now that Zheng Jianguo has poked out the HIV virus, his focus will only be more on the side of Massachusetts General Hospital in the future, so the rest is Celia's laboratory, and after hearing Aurora's official notice, Zheng Jianguo found that if this laboratory is taken back, it will bring him a lot of trouble.

I don't know what Qian Yunjia thinks, Tao Ye, Yi Jinzhi and Wei Yongqiang, who Ye Minde brought with them, can't return to China early, because the visas of the four of them are all applied for in the name of this laboratory, and if the laboratory is gone, it will definitely attract the attention of the Immigration Bureau.

Although Zheng Jianguo can find someone to accommodate, no one likes to make trouble for himself.

Fortunately, MIT has no intention of taking back the laboratory, Zheng Jianguo's biggest worry is gone, and he answers this question just by pushing the boat: "Forget it, the college can terminate the agreement." ”

"Okay, I'll arrange for the legal department to pay you the compensation after completing the formalities. ”

Aurora's voice disappeared from the phone, Zheng Jianguo didn't expect that there was still compensation to be paid, and then Celia's voice came from the microphone and said, "Boss, Professor Ye said that Dr. Qian has agreed to accept the invitation of the laboratory——"

"Okay, I'll go over when I get off work—"

Zheng Jianguo was not too surprised that Qian Yunjia could accept the invitation, since he began to pay attention to the polymerase chain reaction at the beginning of the year, he has been collecting the latest progress about this thing during this time.

The reason why it is the latest development is because Zheng Jianguo already knew that as early as 1971, Crapp, a disciple of Indian Nobel laureate Krana, germinated the idea of studying polymerase chain reaction technology.

And in the same year, Crapp published this idea in JOMB (Journal of Molecular Biology) in the form of an article: "If a double-stranded molecule wants to obtain two identical structures, and each contains the full base length of the template strand, including primers, DNA polymerase needs to be added to complete such a repair replication process."

Eventually, one original double-stranded molecule produces two identical double-stranded molecules, which need to be repeated over and over again throughout the cycle, and each time a fresh, energetic polymerase is added. ”

Crapp's idea is good, and the basic process of denaturation-annealing-extension described in his article is the basic principle of PCR technology, and the reason why it is said is that the most critical mechanical automation is missing.

Just as Ye Minde got this article, he quickly completed the repeated test according to the method in the article, although there were slight differences in the results, but after all, the purpose of the test was to verify whether the technical means were effective, not whether the results were correct.

As a result, Ye Minde just said that the test was repeated at that time, and when this sentence fell on Zheng Jianguo's ears at that time, he thought that this test was relatively simple.

It wasn't until he chatted with Tao Ye a few days ago that Zheng Jianguo knew that this experiment was easy to say, and it would cost the researcher his life to do: "The three steps of denaturation-annealing-extension are for the researcher to carry a small basket containing a test tube and soak it in three water baths for a period ranging from ten seconds to a few seconds to dozens of seconds, which completes a cycle——"

Considering safety factors, Zheng Jianguo did not dare to say that the purpose of his research was to find high-temperature polymerase and then conduct research on mechanical automation.

Of course, Zheng Jianguo knew from that time that this was the common treatment of scientific research migrant workers, and it was also the main reason why Krep wanted to engage in PCR technology in 1971, but unfortunately no one had done it in 1980.

And the reason why no one can figure it out, Zheng Jianguo also knows the reason from Tao Ye's mouth: "——DNA polymerase and modified nucleic acids that can stop the extension of reaction primers can be used for DNA sequencing experiments, and this DNA hybridization detection can be used for semi-quantitative detection of target DNA fragments, and the teacher used this method to complete the detection of repeated test results——"

"End-of-strand termination DNA sequencing!"

This is a technical invention that Zheng Jianguo is more familiar with, which does not mean that he knows more about gene technology, but because the "Sanger detection method" invented by Nobel laureate Sanger is the most popular innovative invention of this year's Nobel Prize.

As a man who aims to win the Nobel Prize, the biggest difference between Zheng Jianguo and other researchers is that he will combine the memories of his previous life to analyze the inventions and discoveries that may be his opponents, and this sequencing method invented by the Nobel Prize winner is the Mount Everest mountain range that he feels invincible: "This is the key to the door of PCR technology!"

With sequencing, you can study the DNA fragment you are interested in as much as you like, as long as you follow Crapp's method to denature, anneal, and extend dozens of times, you can use this method to detect and do what you want to do.

However, in Zheng Jianguo's view, the reason why the Sanger test is so important is that when you repeat the Crapp test, you will have unpredictable consequences that interfere with the accuracy of the test results due to the different activities of the polymerase you choose to use.

And this is just as the reason why Qian Yunjia resolutely gave up her original job after being invited, and of course Zheng Jianguo's treatment conditions: "2,600 US dollars after full tax for food and accommodation——"

In the cross-bridge rice noodle shop, Zheng Jianguo invited everyone in the laboratory to come over for dinner, and there were Celia Ye Minde and Qian Yunjia sitting at his table, and the others were sitting at four full tables except for the two who had a date.

It's just that after Zheng Jianguo said the conditions he offered, he didn't expect Qian Yunjia to ask: "This treatment is relatively good, but I want to ask if there are any other biology positions in your laboratory - my husband teaches at Montana State University, and I think if there is a position in the laboratory, I can recommend him to try." ”

"What field of study is your husband?"

Zheng Jianguo didn't expect her to ask this question, but think about it, this big sister came up with high-temperature polymerase 4 years ago, and now how can she get married after graduating four years ago.

Of course, this is not a big problem for Zheng Jianguo, and if the position is not a position, it depends on whether he wants to add it: "Now there is another project in the laboratory, which studies the effect of nitroglycerin on blood vessels——"

"My husband studied neurobiology when he was in school, and he has a master's degree in neurobiology from Montana State University, so I wonder if there are any suitable positions in your program?"

Qian Yunjia's melon seeds showed concern on her face, but Zheng Jianguo's eyes stopped on the cross hanging around her neck, and then looked at Professor Ye Minde next to her with a smile, and then turned around and said: "That project still lacks an experimental assistant, and the treatment is similar to yours, and I also know that MIT is preparing to establish a bioengineering major, you can also let your husband try——"

"Yes, thank you!

Qian Yunjia nodded slowly, and after saying with piety on his face, Celia's gaze quickly fell on Zheng Jianguo's face, and after looking at the wonderful look on his boss's face, he looked at Ye Minde, and then heard Zheng Jianguo laugh dryly and said, "Well, for you, I want to thank God." ”

Because he had already read her resume before, Zheng Jianguo was already familiar with the performance of these people with pious beliefs, and then beckoned everyone to dinner, first of all, there were people at the next few tables to say goodbye and leave, and finally when Celia also left with Qian Yunjia, Ye Minde looked at Tao Ye and a table of people next to him with a smile, and said, "I'm afraid you haven't eaten enough, right?"

"If you haven't eaten enough, let the boss continue to go up-"

Zheng Jianguo said with a smile and then recruited the boss, and after ordering four servings of rice noodles, he watched the boss leave, but he didn't expect Ye Minde to open his mouth and say: "Jianguo, if you get both of them in, aren't you afraid that the man is an underground worker?"

"Now the above treatment of the other side of the sea is mainly Huairou, it doesn't matter if it's not big, I will never let them go to work in China, and Qian Yunjia is more useful for our projects.

Actually, I was going to ask her husband to help you too, but I thought that I wouldn't tie you up to the other side of the sea, so I arranged for the two of them to be separated——"

Zheng Jianguo remembers very clearly what the state of the two sides of the strait will be in the future, but at present, there are not many people who are concerned about this project, and it can be seen from the attitude of MIT towards this laboratory, that is, he does not believe that he can study anything from the effect of nitroglycerin on blood vessels, and as for the research on polymerase, he will not be worried.

"The four of us are useless, and we're not celebrities, but you, don't let people get you over there-"

Ye Minde looked at the boss and brought the rice noodles with a dinner plate, and saw Zheng Jianguo also watching the boss put down the rice noodles, and said, "Boss, what about that waiter of yours, why did you do it yourself?"

"I've found my mother-in-law's house, I haven't come here yet, Jianguo, you eat first, let's talk when we're free, I have to clean up the table-"

The boss who put down the rice noodles said in a hurry, Zheng Jianguo nodded and didn't say anything, but looked at Ye Minde next to him: "It's useless to get me over, my father and mother are both in China, and besides, they don't dare to do it at this time."

Now that the United States is going to disassociate itself from them, they will come out like this again at this time, I'm afraid that the friends will be more than frightened, do you want to eat some more?"

"I don't want it, what you eat is yours-"

Seeing Zheng Jianguo's inquiring eyes, Ye Minde pushed the rice noodles in front of him, and then glanced at the few people who were eating quickly on the table next to him, and after he sighed and endured a few bites, he said: "Remove the factors over there, you also have to pay attention to the wealth is not exposed, when you said that Dr. Qian was treated before, I saw that the person who was eating glanced at you, and besides, you are wearing like a bag now——"

"I've been paying attention to this, but when you say this, I feel that you have to take care of Tao Ye and them, and when the subsidy is issued, you'd better put it away for them, don't be greedy for cheap things to buy and run away, the security situation in this area of Boston is still relatively good overall-"

Hearing Ye Minde's reminder, Zheng Jianguo looked at the few people on the table next to him as if he remembered something, the highest salary in his previous laboratory was Celia, who was almost full-time, less than two thousand dollars per month after paying taxes, and the others were all part-time parts, and after paying taxes, they were six or seven hundred, seven or eight hundred.

But Ye Minde and the four of them had to stay full-time for six months, and when Zheng Jianguo gave subsidies to several people, he gave the figure of $1,800 according to the average standard in the United States, while Ye Minde was 2,600 and Celia was $2,000, and the old man didn't say anything after he knew about it.

Because the old man was a 50,000 yuan household two years ago, and there are more than 30,000 yuan left after paying 20,000 party dues, among which Zheng Jianguo's capital for reselling movie tickets and the material cost for finding Helicobacter pylori are all paid by the old man.

2,000 US dollars can be exchanged for 3,000 yuan, and it is 12,000 US dollars in six months in half a year, and 18,000 yuan in RMB, but this is according to the official exchange price.

It's just that Zheng Jianguo knows that the $12,000 can be exchanged for up to 100,000 yuan in the hands of some people outside the Bank of the Capital Republic.

Of course, the old man disdains to change it, just as he won't discuss with Zheng Jianguo what his salary is now, money, Ye Minde is not bad.

It wasn't until Zheng Jianguo's words fell that Ye Minde glanced at him abruptly, and then nodded: "What you said is that when the time comes, you will give me their money directly, and say that I will let you do this, which of them will use me to give them again, remember?"

"Hehe, that's what I thought, when you guys go back, let them buy something they want—"

Eating rice noodles with a big sigh, Zheng Jianguo said after eating it with a hot feeling, he saw Ye Minde probing his hand and pointing at his mouth and said: "Eating something too hot is not good for the esophagus, you still told me this, why did you forget it yourself now? Wait until it is cold before eating——"