Volume 3 Alienation Begins to Emerge Chapter 135 The Value of Living Mutant Animals

"Cangchi honorable guest, I hope you can treat it well. The head of the Murphy family seemed to be bidding farewell to his daughter at this time, and the reluctance was beyond words.

"The spirit weapon has a spirit, and I will naturally treat it well. For this spiritual weapon, Cang Chi didn't dare to underestimate it, so he could only respond quite cautiously.

As if feeling the call of Cangchi, the Fire-devouring Giant Sword suddenly took the initiative to break free from the shackles of the iron hook and shot directly towards Cangchi. With a crisp sound of the "bell", the heavy fire-devouring giant sword was inserted into the rock in front of Cangchi. Looking at it like this, it seems that he wants to test Cangchi.

Not to mention, just the depth of this fire-devouring giant sword inserted, ordinary people or lizards really can't pull it out, but Cangchi has the existence of the sea knowledge space, and he directly stepped forward and waved his hand, and the fire devouring giant sword was included in the sea knowledge space by Cangchi. The fire-devouring giant sword, which felt like it had been killed by an external plug, flew around a few laps in the sea space of Cangchi, and then hovered quietly in it.

After everything was settled, Cangchi resigned and left Summer Island after a long conversation with the head of the Murphy family about the use of the Fire Devouring Greatsword. After feeling a little dependent, Cangchi began his penance journey again, and time flew by.

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Cang Chi is working very hard to clear the obstacles on her way to becoming stronger, and after Ye Feiqiu captures two live mutant locusts with the help of Cang Chi, there are also a large number of obstacles that she needs to clear.

After the news of the successful capture of the living mutant locusts was transmitted back to the Institute of Life and Biological Sciences of the Federal National University in an encrypted way, a large number of supporting experimental facilities and equipment to imprison the living mutant locusts were in full swing to design and build. The relevant funds are not a problem at all, they are basically allocated in seconds. When Ye Feiqiu returned to the Federal National University with two live mutant locusts, he was the highest level of security protection throughout the whole process.

Many upper-level people who know the inside story have all come to inspect one after another. After expressing satisfaction and praise for Ye Feiqiu's experimental progress, they all left the laboratory one after another. Although they are all very concerned about the progress of the experiment now, professional matters have to be handled by professional personnel, and they know that they are in a hurry.

Last time Ye Feiqiu was in Shapu City, he conducted a systematic study on several dead locust corpses, and found that the super mitochondria in the mutant animals came from plants and entered the animal through the digestive system. Such a conclusion was also verified during the dissection of a large number of microvariant locusts.

Since the corpses of mutant animals decay and die quickly after death, the corpses of three mutant locusts were carefully dissected and placed in DMEM solution to keep them alive in addition to the mutant locusts used for immediate experiments. Judging from the results of today's preservation, the body tissues of locusts that were dissected later and were too large have died, and now only a small part can be left.

Ye Feiqiu also expressed his helplessness about such natural attrition, after all, if the body of the mutant locust was dissected too finely, the purpose of preserving the mutant locust sample would be lost. However, Ye Feiqiu is not such a pedantic person, for the first locust that was dissected in time, it was carried out more carefully, and all the locust body cells were well preserved.

However, even if all locust body tissue soaked in DMEM solution were inactivated, it would not constitute a fatal defect. After all, they have mutant locusts alive, which is one of the values of live mutant animals.

And in the future stage of live testing, these two mutant locusts will also be of great use. A series of experimental inferences can be verified on them, and they are the guiding light for Ye Feiqiu's future research.

Since the device for imprisoning the live mutant locusts had been made before Ye Feiqiu returned, the two mutant locusts had also been locked inside. After solving this most important hidden danger, what Ye Feiqiu needs to do now is to completely prove whether the super mitochondria generated in plant cells can really survive in animal cells.

In order to solve this problem, although the demonstration process is very complicated, the process of preparing plant super mitochondrial extract is relatively simple. At least for their labs.

After breaking the wall of the plant cells, centrifuge them in a 6000 g centrifuge for 15 minutes. Discard the supernatant, then add the cell homogenate, homogenize with a homogenizer or a manual homogenizer tube, level the centrifuge tube containing the homogenate solution and put it into an ordinary centrifuge, centrifuge at 2500 rpm for 15 minutes, slowly take the supernatant, transfer it to a high-speed centrifuge tube, and store it in a beaker with ice cubes. Centrifuge at 17,000 rpm for 20 min, discard the supernatant, and leave the pellet. Add 1 ml of 0.25mol/L sucrose and 0.003mol/L calcium chloride solution, blow it into a suspension with a pipette, centrifuge at 17000 rpm for 20 minutes, suck the supernatant into another test tube, leave the precipitate, add 0.1 ml of 0.25mol/L sucrose and 0.003mol/L calcium chloride solution and mix it into a suspension to obtain super mitochondrial extract.

Because Ye Feiqiu's laboratory has such technical reserves, it is quite simple to obtain the extract of super mitochondria. All that's left is to test the body tissues of the mutant locusts that are still active. When Ye Feiqiu dropped a drop of extract containing super mitochondria into the slice tissue of the mutant locust, she observed the process with a low-power microscope.

The result is very obvious, the super mitochondria suspended in the solution touch the locust tissue cells with the turbulence inside the solution, and the magical scene begins, once these super mitochondria touch the locust cells, they will immediately drill into the cells through the cell membrane. It's like locust cells actively opening a door to super mitochondria. This process is very slow, Ye Feiqiu immediately pointed at this point with a high magnification mirror, and then carefully observed the specific process.

After the super mitochondria passed through the cell membrane of the locust cell, the mixed phospholipid protein membrane actually wrapped the contents of the super mitochondria again to form a super mitochondria and appeared in the locust cell.

Such a process can only be described as incredible! You must know that different species, even comparable species, with slightly different genes, will produce strong rejection in the process of cell transplantation. Now this incredible phenomenon happened in front of Ye Feiqiu, which made her both scared and excited.

If such a mutation result leads to different species being able to devour evolution, then the future journey of mutation evolution may be another scenario!

However, Ye Feiqiu, who was excited, quickly calmed down, because she thought of the super fusion between plant cells. For example, the fusion of plant cells can create polyploid plants, or directly break through reproductive isolation to create distant hybrids. For example, polyploid seedless watermelon, tomato-potato hybrid plant, and so on.

Perhaps it is this super affinity of plant cells that has caused the influence of the ancient god particles to extend from the plant population to the animal population!

Ye Feiqiu, who has achieved phased results in his research, has even thought of the method of manufacturing human mutants in large quantities in the future, and only needs to prepare a large amount of super mitochondrial extract!

Ye Feiqiu, who felt extremely excited, didn't have the slightest intention of stopping, she directly took a relatively complete piece of locust tissue fragment, and then injected a large amount of super mitochondrial extract into it.

At first, the locust tissue was sickly pale, and then when the injected super mitochondria were gradually absorbed, the tissue cells became more viable. However, soon there was a mutation, and although the fast tissue cells were still cultured in DMEM culture, they were also decaying at a speed that was visible to the naked eye, as if the cells had exhausted their stored potential.

The unwilling Ye Feiqiu tried it again, and the result was still the same!

However, the successive failures immediately made Ye Feiqiu realize that it may be the insufficient supply of nutrients in the DMEM culture medium that caused the rapid necrosis of the locust tissue. After doubling the nutrients, Ye Feiqiu experimented for the third time, and the result was that the decay rate of the locust tissue was significantly slower this time. Eventually, however, the tissue cells died.

It can be seen that in addition to the nutrient reasons, the bearing capacity of the cells themselves also plays a certain leading role. This process is like making a child suddenly have the strength of an adult, but his delicate body cannot withstand his own strength. The result is a boom and bust that immediately leads to self-destruction.

It can be seen that it is not possible to achieve rapid variants, such a process must be gradual and should also follow certain quantitative standards. If you want to quickly formulate such a reference standard, in addition to studying the living locusts, Ye Feiqiu suddenly thought of Cangchi.

However, it is still too early to talk about such a project, and it is most important to first study the upper limit of the concentration of super mitochondria.

In order to study the upper limit of super mitochondrial concentration, in addition to testing and analyzing the number of super mitochondria in various tissue cells of mutant locusts, it is also necessary to borrow ordinary locusts for live experiments. This is a relatively detailed and lengthy process, and even if the number of experimental personnel increases and the number of comparative experiments being carried out at the same time, it only makes it a little faster.

Because it is related to the rapid development of human variants, this huge experiment of testing the optimal concentration of super mitochondria is repeated day by day, month after month. In this repeated verification, in the process of continuous improvement of the data, in the smile of everyone's satisfaction, time passed day by day.

In this process, in order to prevent the two captured mutant locusts from continuing to grow stronger during this time, in addition to mixing a small amount of fresh forage, the two mutant locusts are fed refined sugar and protein.

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Spring turns to summer, and the time is soon in the mosquito-infested summer. It is naturally impossible for ordinary citizens of the Federation to know about the existence of the Old God Particles, but this does not prevent them from feeling mosquito bites. This summer's mosquitoes seem to be particularly powerful!

Because ordinary citizens don't know that mosquitoes have become more powerful, so they are bitten all over the body to vent all the grievances in their hearts to the heads of mosquito repellent products, complaints are coming one after another, the phone calls of various manufacturers, and the phone numbers of consumer rights protection hotlines are about to be blown up by the angry masses. Such a hot event was of course warmly received by the media, and a large number of front-page headlines reported it in the form of urban gossip. The collective anger of the masses and the excessive exaggeration of the media made public opinion uproar for a while!

The ordinary people and the media do not know the cause of this incident, and the top of the federation naturally knows. At the suggestion of the above, a large number of experts began to show up and explain the internal reasons for the strengthening of mosquito resistance from various angles. After alleviating the anger of the people, the conclusions of all experts gradually became the same, and the explanation for the stronger resistance of mosquitoes was unified that it was due to the long-term use of insecticides, which led to the targeted screening of mosquitoes, and the resistance of the remaining mosquitoes naturally became stronger.

As for the solution to this problem, the experts have unanimously come up with a very traditional and efficient solution: the use of mosquito nets!

This sentence is simply a sentence that wakes up the dreamer, and the mosquito net factories of the whole federation have made a lot of money. However, because mosquito nets are originally low-end manufacturing industries and have low technical thresholds, the mosquito net industry will not be profitable after a month or two. However, although the mosquito net industry is no longer profitable, all businessmen have begun to target a new outlet, that is, the research and development of new insecticides. Whoever masters the new high-efficiency mosquito killer may be the next richest man in the Federation!

After the Qin consortium was controlled by Cangchi, the Qin father and son all fell into a state of lying on their back, but Qian Hongjie's eyeliner in Cangchi was stationed in the head office, and they couldn't afford to make any waves. Although in the follow-up publicity process, the stock price of the Qin consortium soared again in the form of borrowing Cangchi technology as a shareholder, and the Qin father and son also returned a mouthful of blood. However, even if their assets are now similar to the original, but the actual control of the consortium has changed hands, they are also very depressed.

The dream of regaining control of the consortium has never been extinguished in the hearts of their father and son. Taking advantage of today's high-efficiency mosquito killers, in addition to coaxing Qian Hongjie to get money to invest, the father and son are also asking someone to secretly study their own secret recipes. Since they were big guys in this area before, Qin Dahai, who had an unusually wide network, quickly found top talents in mosquito killer-related industries.

With this hole card that turned the tables, Qin's father and son could even wake up laughing in the past two days!