Without any oath or roar, all the researchers began to carry out the work at hand in an orderly manner, and Ye Feiqiu also led the little assistant Ma Xiu back to his work, everything seemed to be so orderly!

The observation experiment of the baby clone rat king was able to arouse the excitement of the researcher on duty on the first day, but in the rest of the day, aside from the boring observation logs, there were no incidents or anything commendable.

The growth cycle of this young rat king is simply too ordinary to be ordinary, just like an ordinary vole. Seven days later, when the baby rat king just started crawling around, the researchers on duty were still slightly excited, and they slightly stimulated the baby rat king through the mechanical arm of the closed experimental cabin, wanting to see if there was anything unusual about it. It turned out that this generational clone rat king didn't have anything special except for squeaking and screaming.

On the seventh day, Ye Feiqiu also came to inspect the observation records of this one-generation clone, and found that there was no abnormality. She deliberately checked the feeding records of the female rats, and did not find any abnormal trend of increasing food intake. After seeing this mediocre experiment report, Ye Feiqiu had a hidden worry in her heart, she thought that there would be some error in the cloning experiment, resulting in the failure to clone the real rat king.

After all, if this is really a mutant rat king, then this is a little too mundane.

After suppressing the doubts in his heart, Ye Feiqiu decided to continue to observe, and when the mice were weaned at about 12 days old, they should be able to have a phased conclusion. If the baby rat king still didn't show any special signs at that time, she would have to consider whether to redo the cloning experiment.

Since Ye Feiqiu's laboratory had a lot of other things to do besides studying the juvenile clones of the order of the first order, time was fleeting, and on the 11th day, the mice finally opened their eyes. Ye Feiqiu checked the experiment report with an excited mood and found that it was still mediocre. Ye Feiqiu, who was a little depressed, wanted to start the second round of cloning directly. However, she was still able to calm down, and after waiting until the mice were completely weaned and able to eat freely, Ye Feiqiu observed the growth of the mice for three consecutive days.

The results of the experiment showed that the mice's food intake was increasing, but this growth did not escape the normal range of the mice's food intake. After seeing this result, Ye Feiqiu decisively started the second cloning experiment. For this cloning, Ye Feiqiu found the industry's top cloning experts to handle it himself, so as to avoid cloning failure again.

The second cloning went well, the embryo culture and the maternal transfer went well, and now it is only a matter of waiting for the second generation clone to be born. When doing the second cloning experiment, Ye Feiqiu still sent people to observe the experimental data of the cloned mice, and asked people to test the genetic genes of the cloned mice of the first generation.

Testing results have shown that it is indeed a clone of the rat king, but the unremarkable experimental data shows that it is not a mutant animal.

Since the second-generation clone is being cultivated, there is nothing to say about this first-generation order that no longer has any experimental value, and more daring experiments have begun to be carried out step by step on its body.

The first is to test the body cells of the juvenile rat king of the order One Generation to determine whether its cells contain super mitochondria. The results of the test data showed that there were no super mitochondria in all the tissue samples.

For this test result, Ye Feiqiu was a little skeptical that the variation of the animal body had nothing to do with its nucleus, and all the sources of variation existed in the cytoplasm. In other words, the replication of the nucleus dominated by the clone is only a replica of a rat king before the mutation. If you want to replicate the complete rat king, you need to get a mutated female rat king, or inject the super mitochondria in the cytoplasm of the rat king into the egg cells of the mouse.

However, considering the current level of human research on organelles, trying to meet the above two conditions at the same time in the cloning process is almost no different from winning the lottery. A more normal outcome is that when all the tissue cells left by the rat king have been consumed, their cloning experiment is not complete.

If it weren't for the fact that something went wrong in the cloning process, Ye Feiqiu would not have much hope for the second-generation clone now. Because the second-generation clone is also very likely to be just an ordinary little mouse.

In order to further research, Ye Feiqiu decided to transplant the stump of the rat king's ears, which had been kept in DMEM culture medium and still maintained high activity, into the body of the first generation of rat king clones. Because it is a clone itself, the transplantation of ear tissue has been quite successful. Almost immediately after the transplantation, the ear tissue of the original rat king that had been transplanted began to heal with the ear tissue of the clones of the first generation.

With the transplantation of this small piece of rat king ear tissue, something magical happened immediately. The clone of the generation, which was still in its infancy, became noticeably more excited, and its appetite skyrocketed in a short period of time. In order to prevent the first-generation clone from having problems due to frenzied eating, the researcher in charge of the breeding only dared to adopt the strategy of eating less and eating more often.

Since the clone has always been in a state of extreme thirst for food, it has been squeaking around the feeder. Seeing this obvious change in the clone of the first generation, Ye Feiqiu felt that it was not because it was hungry, but because of the body's instinctive need for various nutrients.

In other words, the current generation clone does not need glucose, but a series of nutrients that make up the cell, such as proteins, which are extremely lacking during the growth process.

"You, first increase the proportion of protein feeding.

You, go and break up the meat and add it to the feeding equipment. ”

Ye Feiqiu neatly instructed the researchers to change the nutritional composition of the feeding.

With a large amount of protein, the mice were significantly better at hunger and less fed. After seeing this phenomenon, Ye Feiqiu also came to a general conclusion, after transplanting the body tissue of the rat king, the growth rate and metabolism speed of the mice were instantly enhanced. It's as if the Rat King's abilities have suddenly awakened in this clone.

The experiment soon came to the second day in the excitement, and what made Ye Feiqiu feel excited was that the ear tissue that had just been transplanted yesterday had completely healed into the body of the first-generation clone. The little mouse also grew larger in the frenzied eating, completely surpassing the normal mouse of the same period.

Seeing this abnormal reaction, Ye Feiqiu can now be sure that the key factor causing animal mutation is not in the nucleus, but in the cytoplasm of animal cells. In other words, it is the genetic material carried by the mitochondria in the cytoplasm that dominates this variation!

Considering the ratio of genetic material carried in the sperm to the egg, if you want to successfully clone a mutant, you can only use the egg cell of the mutant mother, and as for the selection of the nucleus, you don't even need to be a mutant or not!

However, such a conclusion is only a conjecture, and the specific verification needs to wait until the maternal variant is captured in the future, and the viable egg cells can be obtained from it. Until this conclusion is confirmed, it is just a mature scientific inference.

For this first-generation clone that has begun to take on the style of the rat king, Ye Feiqiu can only let his research team continue to observe and study. And after knowing that the transplant is effective, the native tissue of the rat king preserved in the DMEM culture medium is very precious. If a powerful mutant can really be cloned like this, then the value of this piece of Rat King's body tissue will be immeasurable!

It may even become the object of scramble for some people with ulterior motives!

Ye Feiqiu's research has made earth-shattering progress, and the atmosphere in the entire laboratory seems to be very high during this time, but this excitement did not last long, and the process of the experiment soon changed again.

After transplanting the ear tissue of the primitive rat king, the rapid mutation process came to an abrupt end after maintaining it for just three days, and this generation clone of the rat king, which should be a juvenile mouse according to the time scale, is now the same size as an ordinary adult mouse.

When the clone reaches this size, its food intake becomes stable and its growth rate slows down, as if everything is in equilibrium.

If this genogen has reached its growth limit, then this genital clone is in a sense a medical miracle, after all, it greatly shortens the growth cycle of mice.

In the process of continuing to study the first-generation clones, the second-generation clones were also born as scheduled. Although the breeding observation report of this second-generation clone with great hopes seemed very regrettable, it did not cause Ye Feiqiu to be overly surprised, because she had already expected it before, this second-generation clone was indeed just an ordinary mouse.