Due to the particularity of prions, Zhou Wen was extra careful when doing experiments.

Biological masks, biological gloves, and biochemical protective clothing are fully armed.

And Qin Haitao and the four of them just helped out, and did not directly touch the utensils containing the virus.

There are currently four ways to detect prions.

1. Electron microscopy.

2. Capillary SDS-gel electrophoresis.

3. Capillary electrophoresis immunoassay of fluorescently labeled peptide chains.

4. Two-color intense fluorescence target scanning method.

The second method, gel electrophoresis, is the best and most straightforward method of detection.

PrPsc is purified mainly by physical and chemical methods - differential speed, ultracentrifugation, sodium N-lauroylsarcosine (lauroylsarcosine), PK treatment.

Capillary SDS-gel electrophoresis is then performed on a "capillary electrophoresis apparatus".

PrPsc, the causative agent of prion disease, was identified based on the timing and shape of the absorption peak at 214 nm as each component passed through the detector.

This method uses a small amount of specimens and does not require immunological reagents, so the judgment is very intuitive.

The disadvantage is that it requires two types of equipment: an "ultracentrifuge" and a "special capillary electrophoresis instrument".

Ultracentrifuges are basically purchased from foreign brands, and the price is as high as 500,000 RMB.

The pron capillary electrophoresis instrument is also very expensive, requiring more than 200,000 RMB.

Even if it's expensive, the key is less likely to be used.

Generally, hospitals can't even make money back for purchasing equipment, and only rich tertiary hospitals like Jiangzhou Hospital will purchase them.

Because of this, medical resources are scarce in large cities.

There's a strange disease in a small city, and you can't even get tested.

According to the physical and chemical methods, Zhou Wen carefully prepared the reagents, extracted the diluents, and put them on the ultracentrifuge to isolate the mutated prion proteins.

The newly upgraded biological immunology made him more handy.

In this process, Zhou Wen is also teaching Qin Haitao and Dong Wenying, telling them what needs to be paid attention to in the process of routine prion physical and chemical tests.

"When you add lauroyl, you must remember ......"

These experiences, not to mention university professors, even experts from domestic prion research institutes are not clear.

Because the research on viruses, especially prions, is still in the initial stage in China, these practical experiences can only be mastered by top foreign research institutes.

For example, the Yeast Prion Research Institute at the University of Kent in the United Kingdom.

For a long time, new discoveries in the field of prion proteins have almost been "monopolized" by several research teams in the field of yeast.

Those top research institutes will not teach you technology easily.

Unless you can get an offer from them.

And Qin Haitao obviously didn't realize how lucky they were, but they just felt that Zhou Wen's explanation was very easy to understand, and even told them the essentials of the action.

When Zhou Wen spoke, Qin Haitao and Dong Wenying both listened attentively.

As for the other two old experimenters, although they were ostensibly listening, they were actually not listening at all.

Because they feel that it is impossible to extract prions with Zhou Wen's method, and such teaching is simply on paper.

Zhou Wen didn't have an "electric eye", how could he know these specific experimental variables, everything was just his guess.

After purifying the PrPsc, Zhou Wen injected the prion-containing clear solution into Beckman P/ACE 5 500 for electrophoresis experiments.

"Okay~" After the injection was completed, Zhou Wen breathed a sigh of relief.

"Qin Haitao, you stay and take care of the machine, and the others wash their hands and eat." ”

When everyone heard this, they went to the sink to wash their hands......

After eating, Zhou Wen didn't dare to go anywhere, and went back to the laboratory directly.

The samples obtained by the conventional extraction method are so rare that it is really unreassuring not to look at them in person......

At half past seven in the evening, the electrophoresis experiment ended.

One of the experimenters, Huang Xingzhong, took the electrophoresis analysis sheet and looked at it casually, subconsciously thinking that this experiment would fail as before.

However, in the next second, a look of surprise appeared on his face, "Hey, come and see, it seems that a prion has been detected......"

When the rest of the people heard this, they gathered around.

"Hey, that's a sigh. ”

"Teacher, come and see, we've detected a prion. ”

"Hiss...... No, how is this possible, is it wrong......"

Zhou Wen took the analysis sheet and looked at it carefully, and soon his face showed irrepressible joy.

No matter how well the previous experiments went well, nothing is 100% right until the results are available.

For example, a malfunctioning capillary electrophoresis apparatus can also lead to a failed test.

This would make all the previous efforts in vain.

So sometimes in experiments, luck also plays a part.

After making sure that it was not dazzling, everyone's faces showed indescribable excitement.

To be honest, neither Qin Haitao nor Dong Wenying thought that prion proteins could be extracted by conventional methods.

It is a matter of great pride to be able to extract prion proteins using conventional collection methods without hope.

After the excitement, Qin Haitao immediately informed Yang Liqun and Zhang Shuguang.

When the two people heard this, they immediately rushed over.

"According to the test, this is an aggregate prion ......"

Yang Liqun and Zhang Shuguang looked at the clear electrophoresis analysis sheet, their faces were full of surprise, joy, excitement, excitement and disbelief.

"Is this really detected in the urine?"

The sample was collected by me and Zhou Wen. ”

Hearing Dong Wenying's words, Yang Liqun and Zhang Shuguang looked incredible.

In the case of conventional and non-routine testing, the extraction of prions has a completely different meaning.

This is a very important discovery and has high research value.

"You're all good. Although this is only a small step in prion research, it is a big step in the field of prions in China. ”

"Thank you, Yang Group, for the compliment. It's mainly because of Mr. Zhou, if he hadn't been insisting, we might have given up as soon as we saw it. ”

Yang Liqun and Zhang Shuguang looked at Zhou Wen.

Both of them have always been very optimistic about this young man, and Zhou Wen has lived up to expectations, showing a very strong talent in biomedicine.

And it often surprises them.

Like this time.

Yesterday they also predicted that it would never be possible to extract prion proteins under conventional collection methods.

As a result, Zhou Wen was "slapped in the face" the next day.

But this face is comfortable.

If they could, they wanted him to continue to slap his face, preferably left and right!

After the excitement, Yang Liqun and Zhang Shuguang went to the computer to analyze the sample collection records, hoping to find out a certain pattern from them.

Zhou Wen also admires Yang Liqun and Zhang Shuguang's behavior very much.

This is the spirit of scientific research.

While no one wants to collect prion proteins in a routine state, you can't stop studying them because you don't want to.

Prevention and control can only be better carried out if we know what causes prion proteins to be excreted through body fluids.

The next morning, Yang Liqun reported Zhou Wen's findings to the hospital and requested the formal establishment of a "prion research team" under the testing center.

The leadership of the hospital held a special meeting to discuss whether to approve the establishment of this research group?

As the First People's Hospital affiliated to Jiangzhou University, whether it is hardware equipment or talent reserves, it is actually not too bad, but why is it not as good as other hospitals?

The main reason is that the Jiangzhou First Hospital has everything and everything is loose, and there is no "unique skill" that can be handled.

For example, the Jinling Military General Hospital in the province is famous in the country in terms of kidney disease.

For example, Huashan Hospital in China Overseas is very good in dermatology, infectious diseases, and neurology.

Another example is Kyoto University Daiichi Hospital, which is invincible in urology.

There is also the orthopedic ...... of Changzheng Hospital

As for the four heavenly groups, North Concord, South Xiangya, East Qilu, and West Huaxi, they are at the level of immortals, and Jiangzhou Hospital can't catch up.

In short, Jiangzhou Hospital can be compared with other municipal hospitals in the province except Jinling, and it is not worth mentioning at all in China.

Because of this, Jiangzhou Hospital urgently needs the same medical technology "town".

In the end, the research team's application was unanimously approved.

Due to the ...... of Jiangzhou City It should be said that in the entire province of the Soviet Union and Eastern Europe, there are no authoritative experts in prions at all.

As the initiators of the virus research, Yang Liqun, Zhang Shuguang and Zhou Wen have undoubtedly become the backbone candidates of the research team.

Under the election of Yang Liqun and Zhang Shuguang, Zhou Wen became the leader of the [prion research team] of the testing center, and the two of them are honorary deputy leaders.

The first group members: Qin Haitao, Dong Wenying, Huang Xingzhong, Liu Shan......

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ps: I'm sorry, please......