Wu Yan knew that Zhou Wen must have a special extraction technique.

But seeing that he didn't want to say it, she didn't force it, after all, this is someone else's housekeeping skill, and it is harmful to leak it.

Then the two talked about the treatment again, Wu Yan told Zhou Wen what she had said to Shi Lei before, and finally asked with a smile: "Do you have any questions?"

Zhou Wen thought of the task and said, "Can you ask, am I a contract worker or a regular worker?"

"Well...... this" Wu Yan hesitated for a moment, she didn't expect Zhou Wen to ask this question.

What is the situation of biology now, she believes that Zhou Wen should know very well in her heart that the contract of the First People's Hospital of Jiangzhou City is so generous, not to mention that he is a small undergraduate, even if he is a master's or doctoral student in biology, many people can't ask for it.

He actually wants to go to the sky in one step and directly solve the problem of compilation, which is really too much, and she is still a contract worker.

Wu Yan thought for a while and said: "Generally speaking, if it is hired by the hospital, it must be a regular worker, but you are an employment contract for the PCR team of the testing center, so I am afraid that you can only be a contract worker." ”

"Contract worker, ......," Zhou Wen hesitated.

In fact, it doesn't matter whether he is a contract worker or a regular worker, as long as he can go to the testing center for internship and practice, he is willing to do it even if he is a temporary worker.

It's not a task to become a full-time worker in a week, and he can't help but stretch his grip.

1000 XP and 1000 points, and a chance to open a black iron chest.

Wu Yan originally thought that the matter had been done, but she didn't expect that there would be extraneous complications here, so she said bitterly: "Although you are a contract worker, the treatment is calculated according to the regular worker, and there is no difference."

In addition, everything comes step by step, you go to work first, and it's not too late to solve the problem after a while. ”

Zhou Wenxiao asked: "You are my senior sister, and you have also been working in the hospital for a year, you tell the truth, if I really sign a contract worker, when will the establishment be solved?"

"Well...... this" Wu Yan was a little embarrassed, and picked up the coffee cup to cover it up.

After putting down the cup, Wu Yan said, "That's it, I'll help you go back and ask." But let's be honest, you shouldn't expect too much. ”

Zhou Wen nodded and said with a smile: "Okay, then it's troublesome Senior Sister." ”

"You're welcome. ”

Zhou Wen was still centrifuging, and he didn't stay long, so he got up and left after a few polite words.

……

When they returned to the laboratory, Yang Yudong and Gu Hongyun couldn't wait to ask him if the interview was successful?

Zhou Wen said with a smile: "I put forward a condition, she said that she would go back to discuss with the leader, and then give me the letter after discussion." ”

Yang Yudong said speechlessly: "The conditions are so superior, do you still have to put forward the conditions?"

Zhou Wen said: "I don't see a rare opportunity, so I want to try their reserve price." ”

Yang Yudong: "......"

He really wanted to use the extraction gun to extract Zhou Wen's brain a little bit to see what he was thinking about all day long?

Zhou Wen smiled and went to do a PCR experiment.

The full name of PCR is Polymerase Chain Reaction.

The Chinese name is "polymerase chain reaction", also known as "polyenzyme chain reaction", is an in vitro nucleic acid amplification technology developed in the mid-80s.

The target gene or a DNA fragment to be studied can be amplified to 100,000 or even a million times in a few hours in a test tube.

The principle is basically similar to DNA replication in human cells, but the reaction system is relatively simple.

Of course, this doesn't mean that PCR is simple.

On the contrary, PCR is very difficult.

First, through high-temperature "denaturation", a piece of double-stranded DNA is dissolved into two single-stranded DNA.

This is followed by "annealing", where the "primers" are allowed to complement and bind to the DNA as a template at low temperatures to form local double strands.

Finally, the DNA polymerase catalyzes the DNA strand with primers as the starting point to produce an extension reaction to turn it into a two-stranded DNA.

Then repeat the process over and over again.

1 to 2, 2 to 4, 4 to 8, 8 to 16, 16 to 32......

Through PCR technology, sufficient amounts of DNA can be amplified from a single hair, a drop of blood, or even a cell for analysis, research, detection and identification.

For example, paternity testing;

For example, vaccine research;

For example, the prevention and control of viral diseases;

Or genetic disease research, etc., all require a large amount of PCR.

Of course, the rice aphid biochemical reagent experiment that Zhou Wen did today is actually not necessary to do PCR.

Aphids are very low-level insects, which are generally directly applied to their bodies with drugs, and then look at their genetic characteristics.

The reason why PCR is still done is more for the purpose of learning PCR.

The PCR reaction consists of water, buffer, DNTP (deoxyribonucleoside triphosphate), primer P1/P2/DNA template, and TAQ enzyme.

When everything was ready, Zhou Wen adjusted the range of the extraction gun, and then extracted 30 μl of water into three sets of PCR tubes.

When aspirating, strictly follow the operating procedures, suck vertically, suction slowly, and release slowly.

After changing the pipette tip, extract 5 ml of buffer into the PCR tube.

DNTP/P1/P2/DNA template was added to the tubes......

Thanks to this period of study, except for being a little nervous at the beginning, Zhou Wen did it in an orderly manner.

After the required raw materials have been added, the next step is centrifugation.

After setting the speed according to the requirements, wait for three minutes for the centrifugation to complete.

Zhou Wen took out the PCR tube containing the mixture from the centrifuge and immediately put it on the thermal cycler for amplification.

Set to 94 °C for 4 min.

Then set up 20 cycle amplification phases.

High temperature, low temperature, medium temperature, high temperature......

1 to 2, 2 to 4, 4 to 8, 8 to 16, 16 to 32......

Zhou Wen waited anxiously, feeling like a husband outside the delivery room, silently praying for "mother and child safety" in his heart, and at the same time thinking, I don't know if it will be a man or a woman who will be born?

At this moment, Zhou Wen suddenly thought that system integration could speed up the progress of the experiment, instead of waiting here for a few hours, why not speed up and see?

No one saw it here anyway.

Thinking of this, Zhou Wen immediately summoned the personal attribute page, and after clicking on the points, an option popped up in front of him.

[Does it cost points to speed up the progress of the experiment?]

[Yes].

[This mission needs to consume a total of 20 points, please confirm again]

Zhou Wen saw that he could speed up the progress of the experiment with only 20 points, so what was there to hesitate about, and immediately chose to confirm.

Before Zhou Wen could react, in the next second, the PCR instrument had already completed amplification.

Zhou Wen was amazed in his heart, the power of the system was simply mysterious.

sighed in his heart, and soon he became nervous again.

The first time I did PCR, I don't know if it will be successful?

Zhou Wen opened the PCR machine and took out the PCR tube from it.

Originally, the following was a product analysis, but Zhou Wen had a true eye, squinted his eyes slightly, and soon he saw the high-density double helix structure of DNA.

“perfect~”

If he hadn't been wearing a mask, Zhou Wen would have laughed.

Who didn't learn to do PCR after countless failures, and who could be like him, the first time he did amplification, and he succeeded at one time?

And the amplification effect is surprisingly good, it's just perfect.

……

Jiangzhou City, First People's Hospital, Testing Center, PCR Group.

Wu Yan returned to the hospital after having lunch outside, and then reported the situation to the team leader's office as soon as possible.

"What about people?" asked Zhang Shuguang, who was writing the experiment report, without raising his head.

Wu Yan was a little embarrassed and said, "That...... Not here yet. ”

When she went, she patted her chest and promised that she had caught it, but the cowhide blew up.

Zhang Shuguang looked up and said unexpectedly: "What, don't you want to come?"

Wu Yan shook her head and said, "No, it's just that he said ......"

Wu Yan told Zhang Shuguang about Zhou Wen's request.

Zhang Shuguang took the pen with his left hand, tapped on the desk, and said with a smile: "You said that you, such a smart person, why did you drop the chain at a critical time?"

Wu Yan said strangely: "Why did I drop the chain? He wants a formal job treatment, how dare I overstep my responsibilities and promise for you?"

Zhang Shuguang said with a smile: "What's wrong with Yue Ling, he is a biology undergraduate, he dares to ask you for a career establishment, don't you want to think about why?"

"Why?" Wu Yan was puzzled, and quickly reacted, surprised: "Oh~~~ You mean ......"

Zhang Shuguang smiled and said: "You have come to find someone, as long as it is not too outrageous, you will promise him first, and if there is any problem, I will talk about it later." It's not a verbal promise, and it's not a package ticket, right?"

"Uh-huh~ Boss, I know it's wrong. Wu Yan nodded again and again, admitting her mistake, "Then I'll call him now?"

Zhang Shuguang shook his head funny, "I'm back, what's the hurry, I'm afraid he won't be able to escape." Anyway, we are also a hospital in Jiangzhou, so we can't be too underestimated, let's talk about him for two days first. ”