The dander was under the electron microscope and nothing unusual was found.

The big stone in Zhou Wen's heart was lowered by two-thirds.

However, he will not be completely relieved until tomorrow when he has tested the mutant aphid and confirmed that there is no virus.

When things were done here, it was just time for lunch.

Zhou Wen came to Shi Lei's side and asked with a smile, "Brother Lei, do you need help?"

Shi Lei, who had just made the electrophoresis gel and was about to kill the guinea pig, said without raising his head: "What's the matter, are you done?"

Zhou Wen smiled and said: "What do I have to be busy with, just go back and change the data casually, you know." ”

It usually takes 2~3 days to run down an electrophoresis process, plus PCR has to be done, and it takes two or three days of hard work, and it is often found that the experimental data are seriously unreasonable.

And then there's f*ck, Jesus, damnit, all the roar.

Then if you don't plan to do it all over again, you're going to have to fake the data.

This is basically the unspoken rule of living creatures, and most living creatures have done this.

Shi Lei laughed twice, he was also from here, nodded and said, "Since this is the case, then you can go back and rest first, and I can do it myself." By the way, by the way, bring us a meal. ”

Zhou Wen turned around, leaned against the test bench and said, "What are you doing when you go back, you don't have a girlfriend." ”

Lin Jiayi, who was wearing a white coat and looking at the microscope with her back to them, turned around and gossiped: "Hao Zhouwen, do you still have contact with Qu Fangfang?"

Qu Fangfang turned out to be the most beautiful among the girls in the laboratory, with a gentle personality and a small jasper, and she was the ideal wife candidate in the minds of boys.

It's a pity that I went for an internship after a year.

Zhou Wen replied: "I haven't been contacted, what's wrong?"

"Nothing. Lin Jiayi said casually, and then grinned: "Hao Zhouwen, you invite me to dinner, how about I be your girlfriend?"

Zhou Wen pouted and said, "If you women have a man who speaks, my child will be playing soy sauce now." ”

Shi Lei smiled.

Lin Jiayi also turned around with a smile.

Just kidding, she's in her second year of study, and she knows exactly what kind of boyfriend she should find.

Here, Zhou Wen turned his head and smiled: "Brother Lei, do you want to help? ”

As soon as Shi Lei heard that he was going back to watch the drama, he looked up and said with a smile: "Then you better stay, it just so happens that Lan Lan asked me to go to the movies in the afternoon." ”

Zhou Wen urged: "Then why don't you hurry up and go back to pick up and pick up, and strive to successfully intubate at night." ”

Shi Lei smiled innocently.

is similar to Zhou Wen, Shi Lei's family conditions are not very good, plus he looks like a blind monk, and he can't talk sweetly, he is about to graduate from graduate school, and he hasn't even kissed a girl's mouth until now.

It's rare to meet a girl who doesn't dislike him, and everyone is very happy for him.

I also recognized it.

"Am I really leaving?"

"Go ahead. ”

Shi Lei handed the guinea pig in his hand to Zhou Wen, turned around and walked out of the laboratory.

"Remember to bring us food. ”

"Know ......"

Here, Zhou Wen pinched the mouse's neck with one hand and pressed it on the console, grabbed the mouse's tail with the other hand, and lifted it up, "Click", and gave the mouse a "cervical decapitation method".

There was no pain at all.

Then quickly disembowel, use scissors to cut off the mouse thigh bone, peel off the muscles and fascia outside the leg bone.

After rinsing, the thigh bone is cut, the bone marrow is extracted, and the thigh bone marrow is rinsed with a hypoosmotic solution, and finally the irrigated "rinse solution" is loaded into the test tube in equal parts for each plant.

Then a mouse was strangled to death, after the bone marrow was pumped, the rinsing solution was prepared step by step according to the above steps, and the previous rinsing solution was prepared, and the weight was matched according to the AB composition and then centrifuged on the centrifuge to start centrifugation.

The genes of mice are 95% similar to those of humans, so many large laboratories use the genes of mice for various drug tests.

However, a gene extraction process usually takes 2~3 days, and those large-scale laboratories and research institutes that pay attention to efficiency will hand over the gene extraction work to the laboratories of major colleges and universities.

A mouse can theoretically extract 4~8 gene fragments, but normally 4 strains can be extracted.

Of course, these are chargeable.

At present, the market price of a plant is about 400~500.

……

As soon as the centrifugation was completed, Shi Lei came back with the packed rice.

After eating in the office outside, Zhou Wen prepared to do electrophoresis analysis, commonly known as "running electrophoresis".

I had my first exposure to this in high school.

It is to make a gel with agarose gel and let the sample run electrophoresis on the gel plate, so it is also called "running gel", which can be used to detect DNA strands.

In addition to running electrophoresis, another method is electron microscopy observation, which is much more time-saving than running electrophoresis.

However, in the case of electron microscopy, it is necessary to prepare glass slides and stain genes, and in this process, time, technique, temperature, and possible contamination may lead to extraction failure, thus losing all the previous efforts.

So novices are generally honest running electrophoresis.

Zhou Wen took the extraction gun and prepared to "blow" after extracting the supernatant, and at this moment, he suddenly thought of the "True Eye".

Want to see what True Eye says about DNA?

He lifted the test tube in his right hand and shook it twice against the light in front of the console, while his eyes narrowed slightly, staring at the liquid in the tube.

What made Zhou Wen unbelievable was that the image of the DNA strand of the white mouse actually appeared directly in front of his eyes, including the broken covalent bonds.

"I'm Nima ......"

Zhou Wen was so excited that he almost didn't jump.

His eyes can see DNA clearly, doesn't that mean that his eyes have caught up with the electron microscope?

He immediately put down the centrifuge tube in his hand, took off the rubber gloves and spread out his hands, his eyes narrowed slightly, and imagined the "true eye" in his mind.

With a "bang", his hand was as clear as a slide under a microscope.

But the next second he couldn't be happy, and he saw that his palms were full of staphylococcus, Staphylococcus aureus and long strips of E. coli......

There are all sorts of other bacteria and microorganisms crawling around in the palm of your hand.

In addition, the inside of the nails is like a nest of bacteria, and it is crowded with all kinds of bacteria, and it looks very disgusting.

I've washed my hands, I really don't know how many bacteria will be there if I don't wash my hands?

"~" Zhou Wen shook his hands twice in fright, and then remembered that bacteria can't be shaken off, so he hurriedly ran to wash his hands again.

He knew that there were a lot of bacteria on the human hands, but they were all made from bacteria and then observed under a microscope.

Because the human hand is not transparent to light, there is no way to see it directly.

Because of this, he has never been so visually stunning, and for the first time, he has realized the importance of washing his hands.

Zhou Wen rolled up his sleeves, first washed his hands with alcohol-based hand sanitizer, then found nail clippers, carefully trimmed the nails that were hiding dirt and dirt, and then carefully washed his hands again.

At this time, when you look at it with the true eye, more than 90% of the bacteria on your hands have been destroyed, and only a small number of stubborn bacteria are still attached to the fingers and skin folds.

Zhou Wen said speechlessly: "No wonder experiments often fail, just because of the number of bacteria in hand, the success rate is high." ”

Back in the lab, he looked at the rubber gloves on the table with his true eye, and found that they were also crawling with bacteria.

"Huh~" Zhou Wen casually picked up the inoculation stick on the table next to him, picked up the gloves and threw them into the waste bucket, and then found a pair of sterile gloves to put on.

After everything was done again, Zhou Wen excitedly picked up the Group A centrifuge tube containing the gene fragments of the mice and observed it again.

Not surprisingly, a clear DNA image appeared in front of my eyes.

This shows that the gene extraction of the mice was successful.

The purpose of electrophoresis and electron microscope observation is to check whether there is DNA in the test tube.

Now he can see the DNA directly, so to speak, without the most cumbersome and failable procedure.

Then he picked up the B set of centrifuge tubes and looked at it, but this time he didn't see the DNA strands.

Zhou Wen said to himself: "Could it be that the extraction failed?"

After putting it down, I picked up another tube of serum in group B, squinted my eyes and looked at it, but still didn't see the DNA strands.

He picked up one of the tubes from Group A again, but this time he saw it clearly.

This also shows that group B really failed to extract.

"Obviously, all the processes are exactly the same, I don't know which link went wrong?" Zhou Wen frowned and pondered.

He decided to do an electrophoretic analysis to see if what the True Eye saw was true, and then to investigate the mistakes in the operation......

——

PS: I know it's wrong, forgive me.