After the greetings, Zheng Lina said half-jokingly and half-seriously, and proposed to work here for Zhou Wen.

Zhou Wen was a little surprised.

He was about to hire another employee, but he didn't expect it to be delivered to his door.

Originally, there was an assistant from Tang Xin, which was almost enough, but he was busy recently, and he didn't have time to extract high-concentration DNA.

So he plans to train a successor to help him make high-concentration DNA.

Tang Xin is naturally the best choice, he knows the roots, and his personality is relatively calm, which is very suitable.

Zhou Wen happily agreed to Zheng Lina's request to stay.

"For the specific work content, let Tang Xin teach you when you go back. ”

Zheng Lina was naturally overjoyed, and nodded again and again, "Mmmmmmmm, I know~ Thank you." ”

Zhou Wen then said to Tang Xin: "Today I will teach you to extract DNA. ”

"Ah...... I'm afraid I won't be able to learn. ”

Tang Xin was a little surprised when he heard this. Although she had dissected mice in college, she mainly did pharmacological experiments and did not extract DNA.

"Hehe, it's very simple, not as complicated as you think. ”

Just do it, Zhou Wen immediately started on-site teaching.

I took a mouse from the cage and came to the console and said, "First of all, kill the pig, this is a little different from your pharmacological test, we mainly take the marrow of the mouse from the thigh bone ......."

Zhou Wen taught Tang Xin how to extract more mouse bone marrow.

He lectures very slowly and meticulously, and these experiences are all obtained by theory and practice after he has acquired the system, which is more simple and easy to understand than any professor in the world.

Tang Xin can basically understand it once.

Of course, DNA extraction does not rely on theoretical experience.

So after Tang Xin understood the principle, Zhou Wen directly gave up the operating table and let her get started in person, while he corrected the technique on the spot.

The centrifugation time, the method of extracting the supernatant, the number of pipetting, the amount of fixative solution added, etc., are all carefully taught.

And this kind of hand-in-hand teaching treatment, even Qin Haitao didn't get it......

For the next period of time, Zhou Wen basically spent time in the Institute of Pharmaceutical Research.

While teaching Tang Xin the method of extracting DNA, he also did pharmaceutical tasks by the way.

In addition, he pumped the air conditioner to dispense some of the best ratio of low-permeability liquid.

This is the key to ensuring that the highest concentration of DNA can be extracted every time, otherwise only DNA with a slightly higher concentration than the average DNA concentration will be extracted.

A week later, Tang Xin was able to complete the DNA extraction independently. Although the concentration is not high.

But it's also a very remarkable thing.

When the mouse DNA was observed in the electron microscope, Tang Xin was very happy.

"Wow~ Awesome......"

......

......

Saturday, December 29th, the day before New Year's Day.

The Prion Research Institute of the First People's Hospital of Jiangzhou City was officially established.

This is also the second prion research institute in China, and its scientific research strength is recognized as the first in China.

The first director of the institute was Li Wenshuo.

Li Wenshuo, who has been a daughter-in-law for many years, couldn't help but moisten her eyes at the on-site unveiling ceremony.

He has been studying prions since his second year of graduate school, and it has been eight years now, and nothing has been accomplished.

At the worst time, because I couldn't apply for scientific research funds, I had to partner with a few colleagues to set up a stall.

Now that I think about it, it's also a bitter tear.

Because of this, Li Wenshuo is especially grateful to Zhou Wenshuo, who pulled him at the darkest moment of his life and arranged a chair for him on the rocket in the sky, so that he could suddenly become a world-famous prion expert from an unknown person in the domestic academic community!

After taking a group photo with some leaders and scholars who came to attend the unveiling ceremony, Li Wenshuo invited several fellow scholars from home and abroad to the office for a detailed discussion.

As soon as they entered the office, they found a large photo of a person hanging on the wall.

"Hey, this person is so familiar. ”

Li Wenshuo looked at the photo of Zhou Wen on the wall and smiled: "Yes, he is the inventor of PsC1, Zhou Wen, my idol." ”

Li Wenshuo is not only grateful to Zhou Wen now, he even admires this young man who is nearly 10 years younger than him. That's why he got a photo of Zhou Wen to hang in his office, and he regarded it as chasing stars.

Professor Qin Ziyu, deputy director of the China Prion Research Institute, said regretfully: "He obviously has an outstanding talent in prions, I don't know why he doesn't continue to study in depth?"

Li Wenshuo smiled: "I also asked him about this question.

He said that there are too many diseases in this world, and all kinds of viruses and bacteria are threatening human life and health, which often makes him feel powerless.

That's why he gave up studying prions and engaged in more advanced genetic research, hoping to one day crack the human genetic code and cure more diseases. ”

Qin Ziyu sighed when he heard this: "Although I don't approve of him doing this, but no matter what, his courage is commendable." ”

Li Wenshuo nodded, and then smiled: "This morning, the MIT...... of the United States"

No. 2333, Jiangzhou Science and Technology Park, Institute of Neocryptococcal Disease.

The site renovation here, as well as the equipment and personnel, etc., are all in place.

Fifteen newly recruited PhDs in cell engineering also have a preliminary understanding of the next research project.

And the result of understanding is that everyone is confused.

With the level of these people, it is impossible to clone antigen serum cells;

Not to mention that the content of antigenic B cells in serum is as low as a terrible number, even if B cells are isolated, the requirements for the sensitivity and specificity of their sequence expansion and determination are very high.

Even if the sequence is eventually amplified and the antibody is expressed, the final effect of the antibody is debatable.

If they really have this level, how can they be reduced to the point that they can't find a job, and they would have gone to Europe and the United States to earn dollars a long time ago.

At lunchtime, Xiao Yang, the head of the research group, found Qin Haitao and asked, "Do you know what Zhou Suo's plan is? Now the work cannot be carried out at all, and there is no direction at all." ”

Qin Haitao said with a smile: "Don't be impatient~ You first have a good understanding of cryptococcus and the composition of antibacterial serum, as for how to unfold it, I will explain it in detail when I come back to Zhou." ”

Xiao Yang said helplessly: "Okay......"

Jinhu District Industrial Park, No. 404, Huanyu Institute of Biotechnology.

Unlike Xiao Yang and them, Wang Wei, a doctoral student, has lived a very fulfilling time during this time.

In accordance with the company's requirements, they have a detailed understanding of mainstream biotechnology at home and abroad, such as molecular biology, genetics, bacterial communities, natural products and plant synthetic biology, clinical biological processing, etc.

91 people, a group of three people, and a large group of 30 people, learn from each other, communicate with each other, compete with each other, and initially build a good and orderly academic ecological environment.

Such an ecological academic environment is the dream of all living organisms in China.

However, building such an academic environment requires very high funds.

In the past week, 210,000 yuan has been spent, and the average is almost 3,000 yuan per person.

You must know that this is only the most basic scientific research guarantee, if you go back and wait for the laboratory to open, all the laboratories will be in the laboratory, this cost will be increased by at least 5 times.

1 million a week.

That's 4 million a month.

Don't mention anything a year, the scientific research fund alone will cost at least 50 million.

This is also the reason why Zhou Wen hesitated before.

The task seems simple, but in fact the real difficulty lies in the later business development.

If we can't develop a product and launch it as soon as possible, the research institute will close its doors soon.

At two o'clock in the afternoon, in the office of Hengfeng Building, Chen Zhiyuan looked at the thick pile of bills on the desk, with a painful expression on his face.

During this time, money is like running water, office space, office consumables, equipment and instruments, activity funds, etc., and it is money to open your eyes every day.

If he doesn't know the price of firewood and rice, it is the first time he knows that the boss is under so much pressure.

The first 50 million funds have all arrived, but more than 3 million have already been spent.

This is "small money".

The real big guys are in equipment procurement, and the bill on the table is the equipment list.

A total of 36 million! 2 million advance payments have been made.

Just 10 sets of Hitachi High-tech S-3700N, Hitachi High-tech S-4800, Hitachi High-tech SU-70, etc., the total price of different types of electron microscopes exceeds 20 million RMB.

Terrifying!

After carefully checking the bill, Chen Zhiyuan then went to the finance office and asked them to make the final payment to the supplier.

I just came out of the finance room when the phone rang in my pocket.

When I took it out, it was an unfamiliar local number.

Chen Zhiyuan connected and said, "Hey, hello, I'm Chen Zhiyuan, who are you?"

"Hello Mr. Chen, I am Meng Xiang, general manager of Jiangzhou Tianhai Intellectual Property Agency Co., Ltd., Mr. Zhou's patent application has been approved, and there are some procedures for him to come and hand over. ”

Chen Zhiyuan said strangely: "What patent?"

Meng Xiang: "The process of sildenafil granules. ”

"What, sildenafil?" Chen Zhiyuan exclaimed when he heard this.

Meng Xiang: "Yes! If you have time now, you might as well come to the company." ”

Chen Zhiyuan hurriedly said: "Okay, okay, I'll go over now, where is the address......

Hanging up the phone, Chen Zhiyuan was so excited that he almost didn't jump.

He has eaten that powerful sildenafil granules, and the effect is amazing, if he goes back and produces it, hehehe......

And Zhou Wen said that this drug will be given to Huanyu as an agent.

Thinking of this, Chen Zhiyuan immediately set off for Tianhai Company.

When he arrived at the company, Zhou Wen followed closely.

Then Meng Xiang's words made the two excited hearts feel like they had been poured a basin of cold water, and they instantly cooled.

According to the Food and Drug Administration, the new sildenafil granules already belong to the category of new drugs, and if they want to produce and sell, they need to go through the clinical trial process again.

Zhou Wen's dream of collecting patent fees was shattered.

According to the normal clinical trial process, it takes at least 3 years from human trials to market sales.

Chen Zhiyuan was speechless: "It takes so long?"

Meng Xiang nodded and said, "Yes! ”

Zhou Wen asked, "What about the abnormal process?"

Meng Xiang said with a smile: "There is another way, take the green channel of rapid review, if it works, the clinical trial can be completed in about 12 months."

Because after all, sildenafil is also a drug that has been tested in the market for many years, and on the basis of the same raw materials and formula, the production process has only been improved, and there should be room for operation.

You can talk to CRO about this, they should have a solution. ”

Hearing Meng Xiang's words, Zhou Wen's eyes suddenly lit up, "Oh, how did I forget about them." ”

The so-called CRO is called Contract Research Organization, and the Chinese name is "Contract Research Organization".

It originated in the United States in the early 80s of the 20th century.

It is a commercial institution that mainly provides professional services for pharmaceutical enterprises, medical institutions, and small and medium-sized pharmaceutical and medical device R&D enterprises in the form of contracts.

There are three types of industrial chains.

From the discovery of 0-PCC (preclinical candidate compounds).

Validation process from PCC-IND (New Drug Clinical Application).

INDNDA (New Drug Application Application) in human clinical trial phase.

To put it simply, the purpose of CRO is to improve the efficiency of drug development.

You can think of all pharmaceutical companies as e-commerce stores, and CRO is the e-commerce platform.

When it comes to CRO, we have to mention the domestic "Yaoming Kangde" and "Tiger Pharmaceutical".

For example, Yaoming Kangde is the "Ali of the CRO world", and their slogan is - let there be no difficult medicine in the world, and no incurable disease......