Chapter 241: Experiment Failure

Early the next morning, Su Yimo woke up slowly, and subconsciously rubbed "Dacai" with her face, and then Su Yimo froze.

Suddenly opened his eyes, in front of him was a white chest, and he looked up slowly and mechanically, it was a familiar handsome face that could not be more familiar.

And his arms and legs are on his body......

Su Yimo has the heart to die.

Glancing at Ye Xiaoyang, who was still asleep, she quietly removed her hands and feet, and as soon as she breathed a sigh of relief, she heard a chuckle in her ear.

Su Yimo took a breath, slowly turned her head, and happened to meet Xiao Yang's smiling eyes in the last night.

"You... When did you wake up?" Su Yimo said with a little weakness.

Ye Xiaoyang said slowly: "Just when you want to do something to my chest." ”

As he spoke, he pointed to the collar of his pajamas, which was wide open.

"I just want to rub it off. After speaking, Su Yimo couldn't wait to give herself a slap, and quickly explained: "I thought I was in Clear Water Bay, I thought you were Dacai." ”

Ye Xiaoyang raised his eyebrows, "Who is Dacai?"

"Unicorn, a pink unicorn, and a white one called Xiaocai. Su Yimo said, secretly observing his expression.

Ye Xiaoyang was laughed at angrily, it turned out that she used him as a doll pillow.

Su Yimo's eyes turned, smiled a little flatteringly, and said: "Mr. Ye, you see that my sleeping posture is so indecent, you must not rest well at night, why don't I go to the next room ...... sleep?"

Before he finished speaking, he was interrupted by Ye Xiaoyang, "Don't even think about it." ”

His voice was strong and domineering, Su Yimo pursed his lips, moved his body out again, closed his eyes tightly and did not speak again.

Ye Xiaoyang looked at the time on his phone, it was almost seven o'clock, so he lifted the quilt and got out of bed to wash.

Su Yimo rolled back and forth on the bed twice with the quilt, and spit on herself with some chagrin and shyness.

Treat the night president of the dignified Pupil Shadow Entertainment as a pillow, and she is also... Absolutely.

............

Kyoto University, Shaw Building.

The members of the Big Pick Foursome arrived at the laboratory before nine o'clock according to the time agreed yesterday.

Take out the test tube containing the eight groups of culture from the shaker and add one milliliter to the 1.5 milliliter centrifuge tube.

"The numbers should correspond, don't get confused. Zhou Ying said, and then opened the lid of the high-speed centrifuge.

Mu Xueran was responsible for transferring the bacterial solution, and Qu Xiaolin made a mark, covered the centrifuge tube with a plug and put it into the centrifuge.

Seeing her put all the centrifuge tubes next to each other, Mu Xueran stretched out her hand and gestured above the centrifuge tube, and said: "The centrifuge tubes should be placed symmetrically and kept balanced, otherwise the centrifugation effect will be affected." ”

"Ahh Qu Xiaolin sneered.

...

According to the previous steps, the solution containing DNA in the centrifuge tube was obtained, and then the concentration of the extracted 8 groups of DNA was detected by ultramicrospectrophotometer.

He Xiaomeng taught them how to use it in advance, and he quickly got the data.

Mu Xueran recorded their correspondence in a table, which was between 1.7 and 1.9.

After that, the purpose of amplification is to add the reaction to the sample tube, open the thermal lid of the PCR instrument, insert 8 sample tubes, close the lid, and turn on the power.

Once you've set up the program, it's time to run.

Mu Xueran took the solidified agarose gel and put it in the microwave oven to heat and dissolve.

Here, Wu Zixing put the gel tray into the plastic box and inserted an 11-tooth dosing comb.

Wait for the agarose gel to dissolve, and slowly pour it into the gel making box.

"When it cools down, pull out the comb and add samples, right?" asked Qu Xiaolin.

Mu Xueran nodded, thought of something again, and said, "You have to add electrophoresis buffer first." ”

"Isn't it all integrated into the buffer when you add samples like this?"

Mu Xueran looked at her a little helplessly, these He Xiaomeng had already talked about it once.

"Therefore, the loading buffer should be added to the DNA solution to ensure that the DNA can sink, and then the tip of the pipette should be inserted into the bottom of the small hole when dispensing, but the bottom of the gel should not be punctured. Mu Xueran patiently explained.

Qu Xiaolin nodded, "Oh, I see." ”

At this time, it was almost time for lunch.

"How are you going to eat in a while?" Qu Xiaolin rubbed her hungry stomach.

Zhou Ying looked out the window and said, "Order takeout? I don't want to go out when it's so sunny outside." ”

Qu Xiaolin smiled and said, "I think so too, what about you? ”

"Order takeout. Mu Xueran said lightly.

Wu Zixing also said: "Yes." ”

...

So, the four of them ordered a bento box.

Because the experiment only left to run glue and shine glue, it can be completed in about an hour, and they are not in a hurry, and they all brush up their mobile phones in the process of taking out.

Before going out this morning, Mu Xueran had already told Emperor Jiuxiao that he would not go back at noon, and he had nothing to do, so he found the piano block game he had played before, took out his ear power from his backpack, put it on, and played.

......

The summer afternoon is the most likely to be sleepy, after eating takeout, the four of them lay on the experimental table for a while, and only began to continue the experiment at two o'clock.

...

Once the sample has been loaded, electrophoresis can be performed.

Zhou Ying has installed the electrophoresis device, turned on the power switch of the electrophoresis instrument, and set the voltage.

Mu Xue pointed to the platinum wire in the movie trough and said, "There are bubbles here, which means that there is an electric current passing through." ”

Zhou Ying nodded, "Let's clean up the experimental table first and wait." ”

"Good. ”

After about half an hour, the DNA extract would turn blue and the blue band would slowly move from the negative electrode to the positive electrode, but now the blue band is almost gone, and the tinted pores have become colorless.

"Is this normal?" asked Qu Xiaolin.

Mu Xueran shook her head, she wasn't sure, but it seemed to be a little different from the literature she had read.

Wu Zixing said: "Let's dye it first, and then take a look at the glue." ”

"That's all there is to it. ”

...

After the gel is stained, placed in a gel imager, UV is turned on, and the results of the gel run will be displayed on the computer screen.

"Did you run out?" asked Qu Xiaolin.

On the screen, the white fluorescence on the gel is all piled up in the spotting holes, and two or three have moved forward a little, but the color is very light.

Mu Xueran frowned slightly, "Don't we use Marker either?"

"Ahh "Wuzi walked.

Qu Xiaolin had a sad face, "Then what should we do now?"

Mu Xueran pursed her lips, "It must be redone, and the DNA didn't run out, so there's no guarantee that we're right." ”

"Starting from scratch?" Qu Xiaolin's eyes widened.

"If you can't find the cause, you have to start from scratch. Zhou Ying frowned.

Because Mu Xueran was also in contact with this field for the first time, it was a little difficult for her to analyze the reasons for the failure, so she glanced at the three who were a little lost, and said, "I'll go and see if Sister Xiaomeng is there." ”