Shaw Building, Conference Room.

"Okay, a few of you undergraduates will also talk about what you have done recently, the progress and results of your experiments. Tan Xianglei said to the members of the big four.

Because Mu Xueran was sitting on the far left, she turned her head to look at Zhou Ying, Qu Xiaolin and Wu Zixing, and the three of them also quietly glanced at each other.

As soon as Zhou Ying wanted to stand up, Tan Xianglei ordered the most indifferent Mu Xueran.

"Classmate Mu, let's talk about it. ”

Mu Xueran was slightly stunned, stood up in the next second, and was about to speak, Tan Xianglei pressed his hand and said, "Just sit down." ”

"Oh. ”

Mu Xueran adjusted her chair and said softly: "We have completed the pre-experiment, determined that the optimal culture concentration of the strain is 10-6 power, and then the anaerobic culture of the bacterial liquid, picking different colonies morphologically for scribing culture, what we just did in the laboratory is scribing." ”

"What is the optimal concentration for this culture?" Tan Xianglei asked.

"It is the concentration that is most suitable for observing the growth morphology of the colony. ”

"Oh," Tan Xianglei nodded, "How did you get this mushroom?"

Hearing this, Mu Xueran glanced at He Xiaomeng opposite, and said in her heart: Teacher Tan shouldn't know anything about what we do, right?

Seeing that Mu Xue was stunned, He Xiaomeng thought that she didn't know, so she turned her head to look at Tan Xianglei and said, "This is the baby fecal sample collected in the hospital before, they have a special instrument to deal with it, and then the bacterial liquid was directly sent over." ”

Tan Xianglei nodded, and looked at Mu Xueran again, "Then how many kinds of bacteria are you going to isolate from the fungus liquid?"

"Eight. ”

"Can you now know which eight bacteria they are?"

Mu Xueran thought for a while and said: "Judging from the colony morphology alone, there are more bifidobacteria and lactobacillus, and we haven't observed it under the microscope yet, and we plan to observe it after scribing the line to get a single bacterium." ”

"And what are you going to do next after you draw the line?"

"MRS liquid culture was performed to extract genomic DNA. ”

"How do you mention it?" Tan Xianglei asked.

Mu Xueran thought: Teacher Tan is testing himself? So, he replied: "There is a special bacterial genomic DNA extraction kit. ”

can also guess what Tan Xianglei is going to ask next, Mu Xueran continued to speak: "There are steps for genomic DNA extraction in the kit, and the general process can be as stated in the manual." ”

Tan Xianglei participated in their group meeting for the first time, and he didn't usually ask about this, so he really didn't know anything about their experiments, and asked, "What about after mentioning DNA?"

"......" Mu Xueran was a little speechless to the experimental tutor, but still replied seriously: "After that, you need to test the concentration of the proposed DNA, and use an ultramicrospectrophotometer to measure the ratio of OD260 to OD280, which is of high purity at about 1.8, and then you can carry out PCR amplification of the target gene."

After amplification, part of the PCR product can be subjected to agarose gel electrophoresis for further detection, and the other part is directly sent to the company for sequencing, and the obtained 16S rDNA sequence can be compared by Blast in NCBI to determine which strains it is. ”

Tan Xianglei wanted to continue asking, so he heard Mu Xueran say again: "We are responsible for doing this when we participate in the experiment of the big challenge." ”

He Xiaomeng also spoke: "The next is the content of the topic I am preparing now. ”

"Hmm. Tan Xianglei nodded, "Aren't you almost the exam week now? ”

Wu Zixing said: "If nothing else, this week will do." ”

Tan Xianglei smiled, "Then try not to let it have any accidents, by the way, don't you still need to submit a paper? You also have to prepare in advance, isn't there a competition at the end of September?"

The four of them nodded, and Zhou Ying said, "The paper is already being written." ”

Tan Xianglei leaned over slightly, put one hand on the armrest of the seat, and said to Yu Yang next to him: "Teacher Yu, do you have anything else to ask or add?"

Yu Yang glanced at his meeting minutes, looked up and said, "It's nothing, the data and pictures in your experiment must be saved, I hope your next experiment can be successfully completed, that's all." ”

"Okay, if you don't have any problems, we'll break up?" Tan Xianglei said.

Everyone shook their heads silently.

…

Tan Xianglei left beforehand, Yu Yang beckoned to Mu Xueran, and said with a smile: "The last biochemistry class of this semester has also been skipped." ”

Mu Xueran touched her nose and said, "I have something to go abroad." ”

Yu Yang nodded, He Xiaomeng had already told him, so he didn't ask more, "Review hard these days, and strive to achieve the same good results this semester as last semester." ”

Mu Xue snorted and said a little playfully: "Teacher Yu rest assured." ”

Yu Yang chuckled, greeted He Xiaomeng again, and went down.

Mu Xueran and a few people stayed behind to help clean up the conference room.

"Yukizoe, you go back and rest first. He Xiaomeng patted Mu Xueran's shoulder and said.

Mu Xueran gently lifted the chair under the conference table, turned her head and said, "Okay, then I'll go down first." ”

"Go ahead and get some rest. ”

……

went to 708 to get a backpack, and Mu Xueran went back to the apartment.

It was past 8 p.m. when I woke up again.

The bedroom was unlit, it was pitch black, only the faint moonlight shone through the curtains.

Mu Xueran subconsciously touched the side of her, it was cold, and then she thought that Emperor Jiuxiao had not come back yet.

"Goo~~" my stomach screamed, because I only ate a few bites of noodles at noon today, and now I am very hungry and my stomach hurts.

She was the only one in the dim and silent environment, and Mu Xueran suddenly felt a little aggrieved.

Emperor Jiuxiao was not there, so she could only get out of bed to get some food by herself.

There was still some toast in the refrigerator, Mu Xueran looked at the date, and it was still within the expiration date.

didn't bother to cook anymore, Mu Xueran directly put two slices of toast into the toast oven, and fried a poached egg in the pan.

When the toast is done, place the poached egg on a slice of toast, squeeze some tomato sauce, and cover with another slice of toast.

went to the water dispenser to get a cup of warm water, and Mu Xueran ate it directly at the kitchen bar.

Some tastes the same as chewing wax, and it wasn't until I drank a glass of water after eating that my stomach was much better, but there was still a faint pain.

The plates and water glasses were not cleaned up, and Mu Xueran lay back on the bed.

But I had just slept for three hours, and I was completely sleepy at this time.

Mu Xueran turned back and forth a few times, sat up a little irritably, got out of bed and opened the curtains, and opened the window.

The evenings in Kyoto in June are not so hot and humid, and the breeze blows outside, which is somewhat cool.

The breeze caressed her cheeks through the cracks in the window, and the restlessness in her heart was also soothed.

Mu Xueran took off her slippers, sat on the small couch in front of the window, put her arms on the windowsill, and looked up at the moon.

The moon tonight was exceptionally round and bright, and the moonlight was very soft, but she felt a little cold.