"The most common genetic mutations that cause prostate cancer are: AR, 62.7 percent, ETS family, 56.7 percent, TP53, 53 percent, 40.7 percent. Shen Qi drew up a preliminary plan for gene therapy for Han Meng.

According to the results of genetic tests, Han Meng is most likely to die of prostate cancer.

Then start the treatment with the genes related to prostate cancer.

In the plan, it is first necessary to sort out the pathogenic mechanism of prostate cancer at the genetic level.

In addition, BRCA2 gene mutations have been found in 13 percent of patients with advanced prostate cancer. Cui Hualin assisted Shen Qi in this work.

"Among the mutations in non-androgen receptor-associated genes, the PI3K signaling pathway accounts for 49 percent, the DNA repair system accounts for 19 percent, the cell cycle kinase CDKs account for 7 percent, BRAF accounts for 3 percent, and the WNT signaling pathway accounts for 5 percent. "Du Yuan's academic attitude is the same as that of everyone else, speaking with data, and any experimental design is based on proven or predictable data.

"I'm familiar with this part of the analysis. Loss of heterozygosity of PTEN gene decreases PTEN protein expression, leads to genomic instability, and induces high-grade prostatic intraepithelial neoplasia. Yes, that's right, that's it. Tang Yaxing continued.

Wu Junqiang added: "In the case of genome instability, chromosomes are prone to rearrangement and recombination, such as TMPRSS2-ERG gene fusion, which will promote the occurrence of prostate cancer. When there is a homozygous deletion of the PTEN gene, the downstream molecule AKT is continuously activated and induces the invasion and metastasis of prostate cancer...... Hey, guys, that's what I wrote for my master's thesis. At that time, I asked my mentor why gene therapy technology was not widely used in clinical practice since we had figured out the pathogenic mechanism of thousands of diseases, including cancer, at the genetic level? There is a proverb in the West that a moment of life is also life. Isn't it also a good act to save lives by using technology to make a person live for a few more years, or more than ten years? Of course, people who can receive gene therapy can afford the expensive treatment costs. ”

Shen Qi asked, "What did your mentor say?"

"My mentor said two words: negative entropy. To this day, I have not been able to grasp the true meaning of these two words. Wu Junqiang said incomprehensibly.

"It's a pity that Professor Hu died young. Shen Qi sighed, and he actually gave birth to the love of Boyazi.

Wu Junqiang's mentor, Professor Hu, left in his fifties, the true cause of death is unknown, and his family has been looking for clues.

"Director Shen, what is the real meaning of negative entropy? I have checked the literature, and the concept of negative entropy was first proposed by Schrödinger, who was a physicist, so why would my mentor quote the concept proposed by a physicist? What is the relationship between negative entropy and biology? I really don't understand, and I would like to ask Director Shen to explain my doubts. Wu Junqiang asked Shen Qi, the winner of the two-time promise, and he found that Shen Qi and his mentor seemed to have the same academic philosophy, but this concept was relatively profound, so advanced that even Wu Junqiang, a doctoral student who had studied and worked in a famous biological laboratory, could hardly get a key-point.

Shen Qi said: "Erwin Schrödinger's first career was as a physicist, and he also had a second and third careers. Schrödinger was an all-rounder, and he was proficient in more than just physics. Xiao Wu, the question you asked involves a rather complex theoretical system, which is a level more esoteric than genomics, and even I don't know one of them. So let's not discuss Schrödinger's whimsy for the time being, let's return to the topic, our protagonist at this stage is Han Meng. ”

Han Meng hugged his fists and said, "Director Lao Shen and the brothers have bothered. ”

The pathogenic mechanism of Han Meng's gene therapy has been sorted out clearly.

This is followed by a detailed discussion of the technical aspects of operation.

Through gene sequencing, it is first predicted that a seemingly healthy person may suffer from a certain disease, or a certain mutation is detected in someone's DNA, and then the exogenous normal gene is introduced into a specific gene locus on the chromosome in the target cell, and the abnormal gene is replaced with a healthy gene, and the DNA is reorganized to achieve the purpose of treatment.

There are two technical ways to do this: one is to remove the cells from the patient's body, introduce the missing DNA into the cells, and then transplant the processed cells back into the patient's body. This is called in vitro gene therapy.

The second is to inject healthy genes directly into the patient's organs, which is called in vivo gene therapy.

Shen Qi's setting is gene therapy in vivo.

The technical outline is to use a microrobot to manipulate a microneedle to directly inject normal foreign genes into Han Meng's defective cells.

"Then Han Meng, do you agree with our plan?" asked Shen Qi.

"I agree. Han Meng rolled up his sleeves and took the pen and documents handed to him by Shen Qi.

This document is the "Volunteer Agreement for Scientific Research Experiments".

The main terms of the agreement are: I understand the content of the experiment, voluntarily participate in this experiment, and bear all risks and possible consequences, including but not limited to causing new mutations, disappearance of vital signs and other consequences.

Attached: "Han Meng's Current Gene Sequencing Results" and "Han Meng's Gene Sequence Prediction After Receiving Gene Therapy (Case)"

The "Agreement on Scientific Research and Experiment Volunteers" was drafted rigorously, and the rigor is reflected in the text description and attachments.

After Han Meng receives gene therapy, his figure and appearance will not change.

But biologically, he will become a different person.

For example, if the current Han Meng has a criminal record, the case is filed with the Public Security Bureau, and the Public Security Bureau extracts his DNA sample data and stores it in the computer for future comparison.

Then after receiving gene therapy, Han Meng's DNA will not match the DNA he filed with the Public Security Bureau.

The achievements and sins of Han Meng in the past will become history in biology.

The new Han Meng will start a new life.

Therefore, Shen Qi attached "Han Meng's Current Gene Sequencing Results" and "Han Meng's Gene Sequence Prediction After Receiving Gene Therapy (Case)" and asked Han Meng to sign it.

Han Meng has no criminal record, and he has not committed any crimes.

He was helpful, he helped his grandmother cross the street, donated the books he had finished studying to the Hope School, planted a forest on the money pay, and adopted a hen in the Yunnan-Guizhou Plateau.

From a moral point of view, Han Meng is a good person.

Han Meng's highest achievement was the publication of three SCI papers as a single author during his PhD studies in Canada.

In front of Shen Qi, the work of the three SCIs is not worth showing off.

So Han Meng signed and pressed his fingerprint in the signature column of the "Scientific Research Experiment Volunteer Agreement" without regrets.