Mu Xueran glanced at her helplessly, and said to Zhou Ying: "Senior sister, do you want to prepare the wall-breaking lytic enzyme buffer now?"

Zhou Ying nodded, "Well, let's mix it out first, and then store it in the refrigerator, and the TAE buffer and agarose gel that you need to use during the movie should also be prepared." ”

"Then Senior Sister Xiaolin and I will prepare the buffer used for breaking the wall first. ”

After speaking, Mu Xueran took out the reagents needed from the refrigerator, patted Qu Xiaolin's shoulder, and said, "I'll weigh Tris and EDTA, and you go and weigh the lysozyme first." ”

"Okay, no problem. ”

Because Tris and EDTA are used in small amounts, they need to be weighed with an analytical balance.

It is then transferred to a 100 ml volumetric flask to a volume of 100 ml.

"We only need to use one milliliter, and the preparation buffer is only 180 microliters, so what instrument should we use to prepare it?" asked Qu Xiaolin.

Zhou Ying pointed to the cabinet next to Qu Xiaolin's legs, "There is an EP tube in that cabinet, that is, a 1.5 ml centrifuge tube, you can use that." ”

"Oooh. ”

Qu Xiaolin squatted down and opened the cabinet, which was full of centrifuge tubes of different specifications.

took the smallest one and handed it to Mu Xueran, Qu Xiaolin asked, "Is there anything else I need to do?"

"You help me get a 12 microliter Trition. ”

"Okay. ”

Mu Xueran used a pipette to draw one milliliter of lysozyme buffer from the volumetric flask, hit it into the EP tube, added 12 microliters of Trition and 20 mg of lysozyme, closed the cap of the EP tube, and shook with shaking.

Then mark it with a marker and put it in the refrigerator for storage.

Zhou Ying and Wu Zixing also diluted the buffer in the kit.

"The centrifuge and ultramicrophotometer are here, and the things needed to extract DNA and concentration detection are ready, right?" asked Zhou Ying.

Mu Xueran counted again, nodded and said, "Okay." ”

Zhou Ying also nodded, "Okay, there are also primers used in PCR in the refrigerator, so let's prepare the buffers and agarose gels used in electrophoresis." ”

Qu Xiaolin looked at the data on the computer and asked, "Do you want to configure 1000 ml of buffer?"

"Yes, first of all, it is used more, and secondly, the amount of reagent used in the configuration buffer is relatively small, and it is inconvenient to weigh it accurately. Zhou Ying explained.

"Oooh. ”

A fixed amount of reagent was weighed and added to a 1000 ml volumetric flask, and then the volume was set with deionized water.

As soon as Mu Xueran arrived and picked up the volumetric flask and shook it well, Wu Zixing said, "I'll come." ”

"Good. With that, he handed him the volumetric flask.

Mu Xueran looked at the preparation method of 1% agarose gel, suddenly thought of something, and asked Zhou Ying: "Gel or tomorrow again?"

Zhou Ying thought for a while and said, "Let's mix it out today, and then use the microwave oven to heat it when you use it." ”

"That's fine. ”

Qu Xiaolin was in charge of the preparation, Mu Xueran found the plastic box and electrophoresis tank from the drawer, took it to the sink and cleaned it, rinsed it with deionized water again, and put it on the newspaper to control the water.

By the time the preparations were finished, it was already past five o'clock.

"Call it a day, come over tomorrow morning at nine o'clock, it's not too hot at this time. Zhou Ying proposed.

"No problem. ”

…………

Su Yimo's last promotional event was in Kyoto's Higashijo, between six and a half past eight in the afternoon.

Gymnasium, safe passage.

"Sister Momo, the night manager phone. Nana tilted her head and whispered in Su Yimo's ear.

Su Yimo glanced at the staff around her and took the phone from Nana's hand.

"Night-time. Su Yimo said softly.

"I'm at the South Gate. ”

Hearing this, Su Yimo said "ah", and listened to Ye Xiaoyang say again: "Gymnasium, South Gate." ”

Su Yimo's reaction was a little sluggish, and she subconsciously asked, "You... What are you doing at the South Gate?"

In a low-key business car, Ye Xiaoyang chuckled lightly and said softly, "Pick you up." ”

Take you home, Ye Xiaoyang said in his heart.

Su Yimo was slightly stunned, she couldn't tell what she felt in her heart, she only knew that it was a little warm and a little sweet.

"How do you know I'm ......" Halfway through the words, Su Yimo was secretly annoyed, and said in her heart: I must be stupid, he is his own boss, isn't it easy to know his schedule?

Su Yimo coughed lightly with some embarrassment, and asked Nana next to her if the road led to the south gate.

Nana nodded.

"Well, I'm heading out, and I'll be there soon. ”

Ye Xiaoyang hooked his lips, "Okay, I'll wait for you." ”

The corners of Su Yimo's lips also rose slightly, and she said in her heart: This feeling is really good.

……

The end of the safe passage was full of fans, and Su Yimo, escorted by the staff, finally squeezed into the business car.

Nana knew that Ye Xiaoyang was there, so she decisively didn't follow, but went to the back car with a few colleagues.

The flashing lights and shouts outside the car continued, and it was not until the car door was closed that Su Yimo dared to turn his head to look at Ye Xiaoyang in the back seat.

"Night-time. ”

Ye Xiaoyang said to the driver, "Let's go", looked at Su Yimo again, patted the position on his left hand, and said, "Come here." ”

Su Yimo sighed, "I'll just sit here." ”

Ye Xiaoyang frowned slightly, "You want to keep twisting your head to talk to me?"

"......" Su Yimo said in her heart: This is really tiring, but you have to sit next to him...... She'd rather be tired.

So, Su Yimo shook her head, and then she felt that the air pressure in the car was a little low.

Ye Xiaoyang's face was a little dark, he thought that his little Jasmine had agreed to live with him, and he had accepted himself from the heart, but she was still as far away from him as she was before.

Seeing Su Yimo with her head down, she could be seen biting her lower lip from his angle.

Ye Xiaoyang knew that this was her habit, and she would bite her lip when she was nervous.

sighed silently, he didn't want to force her, but if he continued like this, when would he be able to reach her heart.

"Come here. Ye Xiaoyang said it again, his voice a little deep.

Looking up at Xiao Yang's deep eyes in the night, Su Yimo's heart beat violently.

Is he angry? Su Yimo said in her heart.

Lowering his eyes, after waiting for a while, Su Yimo got up and sat in the back row, but he sat on the other side away from Ye Xiaoyang, almost next to the body.

"Sit down. Ye Xiaoyang said lightly.

Su Yimo didn't move, just raised her eyes and glanced at him quietly.

Ye Xiaoyang laughed angrily, and said in his heart: I am not a tiger, are you afraid that he will not eat her?

Suppressing the smile at the corner of his mouth, Ye Xiaoyang pretended to be expressionless and said, "Don't let me say it a second time." ”

Su Yimo moved to the right, then raised her eyes to look at Ye Xiaoyang's face, and moved again.

Ye Xiaoyang hugged her slender waist and pulled the person into his arms.

Su Yimo's heart was shocked, and she was about to struggle, when she felt him rub against her neck and said, "Don't move, let me hug me." ”

His voice was hoarse and tired.

Su Yimo froze.