Chapter 220: Sister is optimistic about you
took photos of the 10 petri dishes one by one, and Zhou Ying sent them to the group of four people.
Turn off the temperature control power of the incubator, and fill the exchange room with some mixed gas to ensure that the pressure inside and outside the box door is equal, and the box door can be opened.
Zhou Ying pointed to the petri dishes in the box and said, "Take out all the petri dishes and wash them, these are not needed." ”
"Good. Mu Xueran nodded, reached out to the handle of the inner door, twisted it ninety degrees, and opened it with a slight pull.
After taking back 708 from all 10 petri dishes, Zhou Ying looked at the time and said, "Now that we know the optimal culture concentration, let's continue the experiment." ”
The three nodded.
"I'll wash these petri dishes first, and you can mix out both solid and liquid MRS media, see what instruments are missing, and sort them out and sterilize them together. ”
Wu Zixing put on his gloves and said, "Then it's still a match between Xue Ran and Xiao Lin, I'll count the instruments." ”
Qu Xiaolin raised her hand, made an "OK" gesture, and pulled Mu Xue to the experimental table where she had weighed the reagents before.
Because it is necessary to use a purer bacterial solution for scribing culture, it is necessary to perform liquid culture on different colonies, and a liquid MRS medium is required.
Compared to solids, MRS liquid medium only lacks agar.
According to the steps in the pre-experiment, put the instruments and reagents that need to be sterilized into the autoclave, set the temperature and time, and start sterilization.
Zhou Ying took off her white coat and gloves, and said: "I still have a class in the first class, so I will go to class first, pour the plate coating after sterilization, and then anaerobic culture, we have also done these in the pre-experiment, so I will leave it to you." If you have any questions, send me a message or find Sister Xiaomeng. ”
"Don't worry. Qu Xiaolin patted her chest and said.
Zhou Ying chuckled, "Then I'll leave first, you must pay attention to safety." ”
…
Sterilize the bacteria, take out the things and put them on the clean bench.
Because he had just taken it out of the autoclave and there were still residual water stains on the petri dish, Mu Xueran lit an alcohol lamp and dried it next to the flame.
Just to cultivate the anaerobic bacteria in the bacterial solution, it is enough to make an experimental group and two controls, so only three Petri dishes need to be used.
After the petri dish was completely dry, and the MRS solid medium cooled down slightly, Mu Xueran and Wu Zixing began to pour the plates, and Qu Xiaolin was responsible for marking and taking pictures.
"Xiaolin, you go to the refrigerator and bring the P6 centrifuge tube. Mu Xueran turned her head and said.
"Okay. ”
Wait for the medium to cool, take 100 microliters of P6 solution on the medium, evenly coat, and apply two more as a control in the same way.
"Alright. "Wuzi walked.
Qu Xiaolin put the mobile phone in the pocket of her white coat, stretched her waist and said, "You can rest for another three days." ”
Wu Zixing took three petri dishes that had been coated and marked, got up and said, "Aren't you studying for the exam?"
"Of course, we have to review, don't we have to take the exam in advance next week. Qu Xiaolin looked at Mu Xueran again and asked, "Xueran, your freshman exams are all in exam week, right?"
Mu Xueran shook her head, "College writing also has to be taken in advance, this Thursday." ”
"Oh, yes, I forgot. ”
The petri dish was sent to 709 for anaerobic culture, and the 708 laboratory and workbench were packed up, and the three left.
While waiting for the elevator, Qu Xiaolin asked, "Xue Ran, where are you going in a while?"
Mu Xueran took out her mobile phone and looked at the time, 2:51, "I have something to do, I want to go to Grace Mansion." ”
"Okay," Qu Xiaolin said with some grief, "I'm going to the library to review obediently." ”
……
Grace Building, 10th Floor Design Office.
The dress designed for Su Yimo has begun to take shape, and the required diamonds have been delivered two days ago.
Mu Xue ran the aqua blue translucent tulle on the shelf behind her to make the fifth layer of the lower skirt.
Because it is necessary to grasp the pleats according to the designed texture, it is very time-consuming and eye-consuming.
This is a delicate job, until Emperor Jiuxiao called her after work, and Mu Xueran still had a small half of it fixed.
Straightening her waist, Mu Xue ran hammered the back of the hammer, reached the mobile phone, and answered the call of Emperor Jiuxiao.
Emperor Jiuxiao has already arrived downstairs.
I can only come over again tomorrow when I have time, Mu Xue said in her heart.
Putting the phone into the side pocket of the backpack, Mu Xueran put the shoulder strap on one side of the shoulder and walked out of the design studio.
After getting into the car, Mu Xueran pulled out the seat belt buckle and rubbed her eyes.
"Sleepy?" asked Emperor Jiuxiao softly.
"No, it's just that I'm fixing the folds just now, and my eyes are a little astringent. ”
"Close your eyes and rest for a while, Ah Zhan is here, Akan will set up a special bureau tonight, do you want to go?"
Mu Xueran closed her eyes, nodded and said, "Go, I haven't seen Uncle for a long time." ”
"......" Every time I heard Mu Xueran call Uncle Ye Zhanbei, Emperor Jiuxiao was very unhappy, but they were not so particular, how they were called before, and how they are still called now.
……
Dijiang Clubhouse, Box 1001.
When Mu Xueran and Emperor Jiuxiao arrived, Xi Shaohan, Xi Shaoxun and Bai Yueyan were already there.
Xi Shaoxun was fighting with Bai Yueyan, and when he saw Mu Xueran coming in, he immediately threw down the pillow in his hand and rushed towards Mu Xueran.
And Emperor Jiuxiao was one step ahead of her and blocked Mu Xueran's side.
Xi Shaoxun glanced at Emperor Jiuxiao with his spare eyes, went around to the other side, and gave Mu Xue a bear hug.
"......" Emperor Jiuxiao's face was a little dark.
Xi Shaoxun didn't seem to see it, and pulled Mu Xueran to sit on the card seat inside, but she was nervous in her heart, and there was a little excitement, after all, this was the first time she ignored the Emperor Nine Owls and snatched people from under the noses of the Emperor Nine Devils.
"Dye Dye, on the day you weren't there, Nuan Nuan and I went to a newly opened theme restaurant in the southern suburbs, the dishes and desserts were delicious, and we would go together when we had time on the weekend. ”
Xi Shaoxun hugged Mu Xueran's arm, his eyes were shining, and he looked at Mu Xueran expectantly.
Mu Xueran nodded, "Okay, I should be free." ”
"How's your experiment going?"
"The results of the pre-experiment are already out, and today is just the beginning. ”
"Well, come on, sister is optimistic about you. ”
Xi Shaoxun picked up a bottle of cocktail that had been opened before on the table, raised it towards Mu Xueran, and said, "I wish you can win a first prize in the provincial competition after the start of school." ”
Mu Xueran chuckled, "I accepted this blessing." ”
At this time, Bai Yueyan came over shaking his red wine glass, and said in a somewhat frivolous tone: "What are the two beauties talking about?"
"You go, you're not welcome here. Xi Shaoxun said with disgust.
"Tsk, little Kaoru, you're not interesting enough, you forgot that you went to drink some time ago... Uh-huh-hmm......"
Before he finished speaking, Bai Yueyan was covered by Xi Shaoxun, who suddenly pounced.
Bai Yueyan took a step back by inertia, and the wine in the wine glass shook and almost spilled.