Chapter 204: The Great Experiment (2)

What did he know?

Her body ......

Mu Xueran's heart was slightly astringent, she probably guessed the reason, but she still needed to confirm it.

Qu Xiaolin saw her head down and stunned, and said suspiciously: "Snow dye, snow dye?"

Taking a deep breath slightly to hide the emotions in her eyes, Mu Xueran looked up at Qu Xiaolin.

"What's wrong with you? What are you thinking about?"

Mu Xueran gently tugged at the corners of her mouth and said lightly: "It's nothing." ”

"Oh, I just asked you, is your boyfriend handsome, and you haven't answered me yet. ”

Thinking of his handsome face that was defenseless to her, Mu Xue smiled lightly, "Handsome." ”

......

At 10:21, the autoclave made a prompt sound, the sterilization time was up, and I waited for about ten minutes until the temperature dropped to 60 before the autoclave was opened.

Zhou Ying put on her gloves, turned off the ultraviolet light on the ultra-clean workbench behind her, sprayed some alcohol on the gloves, looked at Wu Zixing, who was closest to her, pointed to the glass door of the workbench, and said, "Zixing, you can help me open this glass in a while, don't open it too much." ”

"Good. ”

Zhou Ying then transferred the instruments and reagents in the sterile basket to the ultra-clean workbench.

Next, take advantage of the fact that the MRS is not solidified, and quickly pour the plate.

Mu Xueran and Qu Xiaolin also wore gloves.

"Xue Ran, Xiao Lin, you two come and dilute the fungus solution, and Zi Xing and I will pour the plate. ”

As she spoke, Zhou Ying took out the pre-processed bacterial liquid from the refrigerator.

"Good. ”

The clean bench in their lab is a double person.

Qu Xiaolin pressed Mu Xueran to sit in front of the workbench on the right, and said with a smile: "Xueran, you are worthy, I will get you whatever you need." ”

Mu Xueran raised her eyebrows, turned on the lights and fans on the workbench, picked up the watering can next to her and sprayed some alcohol on her hands, before she opened the glass damper to a palm-wide height, enough to put her wrist in.

took the fungus liquid handed over by Zhou Ying and shook it, and said to Qu Xiaolin: "The original fungus liquid is recorded as P1, then take 1 ml and add 9 ml of sterile water to dilute it, which is the concentration of ten negative squares, recorded as P2, and so on, you help me mark it." ”

Qu Xiaolin nodded and said, "Okay." ”

Mu Xueran found the pipette gun that had been sterilized in the workbench in advance, one was 2000 microliters and the other was 10 milliliters, adjusted the volume to 1000 microliters and 10 milliliters, and pressed the corresponding pipette heads respectively.

Draw 1 ml from the original bacterial solution, beat it into a clean 10 ml centrifuge tube, add deionized water to the 10 ml scale of the centrifuge tube, close the lid and shake it well, and change the clean pipette head to take 1 ml.

Handing the centrifuge tube to Qu Xiaolin for her to mark, Mu Xueran repeated the operation just now.

On the left-hand side, Zhou Ying also lit an alcohol lamp, dried the water stains in the petri dish, and waited for the temperature of the petri dish to drop to room temperature before starting to pour the plate next to the alcohol lamp.

The aim is to ensure better sterility.

Mu Xueran diluted the concentration of the fungus to the minus eighth power of ten.

Because the concentration is too high, it is not easy to observe the growth of the colony, and if the concentration is too low, the colony may not grow, so they only need to use P4 to P8 for the pre-experiment.

Once the MRS medium is completely solidified, the coating begins.

It is to use a pipette gun to draw 100 microliters from the centrifuge tubes with five concentration gradients, and then hit them on the five cooled plates, and use a coating rod to push the bacterial solution and spread it evenly.

In order to avoid accidents, Mu Xueran and Zhou Ying painted a group as a control.

"Don't forget to make marks, I'll mark one to five with a red marker, and you use black for snow dyeing. Zhou Ying reminded.

Mu Xueran nodded and said, "Okay." ”

After coating, Zhou Ying got up and said, "OK, just put them in the anaerobic incubator for cultivation." ”

Mu Xueran also got up and stretched, took the petri dish and went to the 709 public laboratory together.

The anaerobic incubator has been inflated and vented in advance.

Zhou Ying turned off the germicidal lamp, turned on the power of the thermostat and the light, and adjusted the temperature to 37°C.

According to what He Xiaomeng said before, ten petri dishes were put into the incubator, and then arranged in two rows in order by number.

"Okay, it's half past eleven, Saturday, we'll just come over at noon on Tuesday." ”

Zhou Ying turned off the lights and said to Wu Zixing: "The boys' dormitory is relatively close to here, you can come tomorrow afternoon to see if you need to inflate again." ”

Wu Zixing nodded and said, "No problem." ”

......

Back at 708, Mu Xueran took off her lab coat and gloves and washed her hands again.

"Xue Ran, where are you going to eat in a while?" asked Qu Xiaolin.

Thinking about the address Xi Shaoxun sent her, Mu Xueran replied: "The ninth canteen." ”

Qu Xiaolin pulled Zhou Ying and said, "Then the four of us will go together, just go back to the dormitory after eating." ”

"Good. ”

......

Canteen No. 9.

Qu Xiaolin, Zhou Ying and Wu Zixing went to the second floor, while Mu Xueran went up to the third floor to find Xi Shaoxun.

Before entering the restaurant, Mu Xueran sent Xi Shaoxun a message that she was about to arrive, so when she just walked around the corner of the hall, Xi Shaoxun waved at her and said, "Dyeing, here." ”

Mu Xueran raised her eyes, followed the voice and looked over, and saw Xi Shaoxun's bright smiling face.

Across from her sat a slender girl with long hair and waist.

Because her back was to Mu Xueran, she couldn't guess who it was for a while.

It wasn't until she approached that the girl turned her head and looked at Mu Xueran with a smile on her face.

It was a melon seed face with a little baby fat, slender eyebrows, clear eyes, a beautiful nose, and delicate pink lips, which were just right to gather on a pure and refined beauty.

"Nuan Nuan?" Mu Xueran said happily, "When did you come back?"

"The day before yesterday, I had a day of jet lag at home, and I came to look for you this morning. ”

Xi Shaoxun pulled her to sit next to her, and said with a smile: "How is it?

Mu Xueran nodded, she was indeed pleasantly surprised.

"School is out for the holidays?" Mu Xueran asked, but she also knew that some schools abroad had early summer vacations.

"Hmm. ”

Xi Shaoxun sighed, and said with some grievances: "I'm so envious, we have to wait for about a month." ”

Mu Xueran patted her on the shoulder and said to Lu Zhinuan: "Nuan Nuan, your exchange study is two years?"

"Yes, I will come back next summer, and when I get my graduation certificate, I plan to study in country F. ”

"Yes, the goal is clear, unlike me, I don't know what I'm going to do in the future. Xi Shaoxun said, shrugging his shoulders.

"Take your time, but you still have a year to think about this at most, and you should be ready for the graduate school entrance examination by the senior year," Lu Zhinuan added: "If you still want to continue to take the exam." ”

Xi Shaoxun nodded approvingly, with seriousness in his eyes, "Well, I have to think about it." ”

Then he turned his head to look at Mu Xueran and asked, "By the way, Dye, what about you, what are your plans?"

"I don't know. ”

Suddenly thinking of something, Mu Xueran said with a smile: "I will probably go to be a personal secretary for Emperor Jiuxiao." ”