Five experimenters entered the observation phase.

The five groups of rats were numbered as Han Meng group, Cui Hualin group, Tang Yaxing group, Du Yuan group, and Wu Junqiang group, of which half were male and half female.

The miracle didn't happen for a while, but the tragedy soon happened.

After the anesthetic effect of the rats in the Duyuan group ended, they died one after another within a day.

The experiment was recorded as follows:

The No. 1 rat in the Duyuan group died at 12:42 with intracranial hemorrhage.

The No. 2 rat in the Duyuan group died at 12:43 with intracranial hemorrhage.

……

Rat No. 10 in the Duyuan group died at 12:51 with intracranial hemorrhage.

To be exact, the 10 experimental rats in the Du Yuan group died one after another within ten minutes, with an average of one death per minute.

The cause of death was the same, both died of intracranial hemorrhage.

"Hey, I failed. Du Yuan was so annoyed that his experimental protocol proved to be a complete failure, and ten of his friends died violently before they could do an IQ test, dying tragically in the laboratory.

Du Yuan can only be annoyed for one minute, he must race against time to dissect the 10 dead laboratory mice, the main work is to dissect and analyze the brain tissue of the laboratory mice, summarize the lessons learned, and accumulate valuable data for the next experimental design.

There are real risks associated with performing gene-editing experiments on living organisms.

Du Yuan just modified one of the genes of the lab rats, which led to the total annihilation of his lab rats in a very short period of time.

"Brother, don't be discouraged, failure is the mother of success. Han Meng comforted Du Yuan, and he said: "You sort out the analysis report of your experimental data as soon as possible, and give your conclusions, is there a problem with the homologous recombination editing method, or is there a problem with your path selection based on this method? ”

While Du Yuan summed up the lessons learned, the other four experimenters continued to observe the rats in their group.

Soon after, Shen Qi joined the ranks of observers, and he noticed that the rats in Cui Hualin's group became very manic, and this group of rats bit whoever they saw biting whom, biting their own kind if they couldn't bite people, and biting the metal mesh of the cage if they couldn't bite their kind.

"Squeak!"

The rats in Cui Hualin's group grinned with their teeth and looked hideous, now they don't like fresh fruits and vegetables, they just want to bite live things.

"Hey, I failed. Cui Hualin sighed, his little friend was extremely crazy after gene editing, and he couldn't do an IQ test at all. The craziest rat actually bit off its own front legs.

The smell of blood drove the rat even more frantic, and it slammed into the metal mesh, trying to break through the cage and bite at the living creatures outside the cage.

Shen Qi patted Cui Hualin's shoulder: "Extract living cells and brain tissue for preservation, and then put them to death." ”

"Director Shen, why don't we observe for a while?" Cui Hualin was unwilling to fail like this, he still had a glimmer of hope.

Shen Qi shook his head: "No need to continue to observe, kill all the laboratory rats in your group." I want to see their raw data, and if I didn't expect, the modification of their SHANK3 gene affects their neurons, and this effect is irreversible, and I am afraid that they will become even more crazy. ”

"Okay......" Cui Hualin tearfully executed his little friend.

Before the execution, Cui Hualin first used nebulized anesthesia to stun his little friend, extracted living cells, and then dislocated and executed, dissected, and preserved various organs and brain tissue.

There is no good news, and bad news is coming one after another.

The rats of the Tang Yaxing group did not show the aggressiveness of madness, but they became extremely autistic, curled up in the corner of the cage and remained motionless, like a hairy stationary mouse.

"Your group of autistic rats can't carry out follow-up experiments, extract living cells, brain tissue and preserve, and then kill them. I want to see their raw data. Shen Qi gave an order.

"It's a pity, it's a pity. Tang Yaxing shook his head and sighed, and then executed his little friend.

One group of rats has died of their own causes, and the other two groups of rats that have behaved abnormally have been sacrificed by the experimenter.

The progress of human science is inseparable from the contributions made by these experimental animals.

Scientific research continues, and rats continue to contribute to the advancement of human science throughout their short lives.

After observation and various physical examinations, the experimental rats in the Han Meng group and the Wu Junqiang group were temporarily normal in terms of physical indicators and animal behavior, and could be tested for the T-maze.

I don't know if it's good news or bad news, but the T-maze test results of the experimental rats in Wu Junqiang's group are the same as before.

This group of rats encountered a T-junction in the T-maze, and their choice was always to go to the right.

The vast majority of rodents have a one-way preference, and the Wistar rats selected by Shen Qi's team prefer to the right.

The route of the T-maze designed by Wu Junqiang is eighteen right and one left.

The Wistar rat walked to the right 18 times in a row, then to the left, and was able to get out of the maze and obtain food placed at the exit of the maze.

The spatial learning ability of the rats in Wu Junqiang's group did not become stronger, they were quite head-ironed, and they always went to the right and never to the left.

In other words, the IQ of the experimental mice of the Wu Junqiang group that has been edited by the SHANK3 gene remains unchanged and is still at an ordinary level.

"The laboratory rats of Wu Junqiang's group are worth continuing to study. Shen Qi made the above instructions.

"Okay, understood!" Wu Junqiang rubbed his hands excitedly, his little friend would not be executed for the time being, his experimental plan was not proven to be the most suitable plan, but it was not completely denied.

The five experimenters personally selected their rats, took care of them personally, and fed them personally, and they had some affection for their little buddies.

A moment of life is also life, and if they can give their friends a way to live, even if they live for a few more days, the experimenters are not willing to kill them with their own hands immediately.

Thirty rats were killed, and ten rats were retained, and the best situation so far is the Han Meng group of rats.

Han Meng designed a new T-maze route, and his first three lab rats achieved better results in the maze than they did before they received SHANK3 gene editing.

"Han Meng's group of lab rats is very promising. Shen Qi finally waited for a good news.

"Brother Meng, this group of rats, their learning ability has become stronger!" other experimenters watched.

Rat No. 4 of Han Meng's group entered the T-maze, and it was a female mouse.

Scientists have verified that females in this category of Wistar rats are more intelligent than males.

The essential reason why female mice are more intelligent needs to be further confirmed.

But even in the intelligent Wistar females, their right-side preference is still evident.

Before gene editing, the No. 4 female mouse in Han Meng's group could only walk out of the all-right-facing T-maze.

In this new T-maze, Han Meng has set up two T-junctions with the right left direction.

After the first three females failed a few times, remembering the correct route and the wrong route, they came out of the maze and ate the food.

Female No. 4 performed even better, failing only twice and then coming out of the maze.

Female No. 4 still has a right preference as the main trait, but at the same time, her learning ability has become significantly stronger.

When it realizes that it can't get food by going to the right, it will make a change and choose to go left at the same intersection.

Cui Hualin was amazed: "The No. 4 rat is quite smart! If it fails at an intersection once, it will be able to remember that the left direction is correct and choose to go left in the next attempt! Brother Meng, it seems that your gene editing method is very reliable!"