Chapter 1096: Experiment Failure
On the top of the mouse's head, there are two protruding balls of flesh that look like they have horns.
Originally, this mouse didn't have horns, but now it does
"We've gene-edited it, that's why it's like this. A voice came.
Zhou Wen turned his head to look, and it was Jin Zeguang who spoke, a proud disciple of Cui Haoyong.
Kanazawa is now a graduate student and is pursuing a doctorate.
"I see. ”
Zhou Wen responded.
"If you're interested, I can tell you about it. Kanazawa Mitsudo.
"Okay. Zhou Wen showed enthusiasm.
Jin Zeguang turned on the computer and explained to Zhou Wen.
This will not be very busy, and many people will come to the laboratory one after another.
This lab is all about working with genes all day long, and many times it has to extract DNA, and doing experiments is a complicated job.
In fact, Zhou Wen has been here for so long, and he is already familiar with many experiments, but he has not had the opportunity to practice.
He couldn't act too anxious, but he believed that as long as he stayed in the lab, there would definitely be a chance.
"Kanazawa Guang, what do you have to say to him, he is a guinea pig, and he still wants to do experiments?"
At this time, a voice sounded.
It was another student in the lab, Kim Sung-san, who spoke.
Kim Sung-san is now a graduate student, in his thirties, and has been studying for a doctorate for several years.
Many of his classmates had already left the school, but he still stayed in the novice village and couldn't get this degree.
And Kanazawa is only in his twenties, very young.
After a long time, Jin Chengshan's heart was a little unhappy.
He is also an old man in the laboratory, and he often directs newcomers to work.
Zhou Wen is a guinea pig, the lowest character in the laboratory.
"Ren Chao is very interested in these, and I will explain it to him. Kanazawa smiled.
"It's useless to talk about it, his IQ can't understand these things at all. Jin Chengshan said disdainfully.
He looked proud and looked at Zhou Wen: "Ren Chao, hurry up and feed my guinea pigs, if you die, I won't be able to spare you!"
"Okay. Zhou Wen was not angry.
There's nothing to be angry with this kind of person.
"I'm going to have another test today, and I don't know when it's going to be a head. ”
"It would be nice if Mr. Cui's project could really succeed. ”
"Gene editing is the trend of the times, and many diseases will need to be treated with gene editing in the future, and we still have potential. ”
A few students chatted.
Their research topic is a section of the human genome, the role of this part of the gene, and what kind of effects can be produced by how it can be changed.
In this way, we can study what diseases can be treated.
Human DNA has tens of thousands of base pairs, and each part plays a different role.
This is the mystery of genes, and until now, humans have not fully grasped the usefulness of each base pair in it.
If we can really study it, we may be able to discover the deepest mysteries of the human body.
And it is based on this that the future human beings have opened the road of human body strengthening.
It's just that it's going in the wrong direction.
After Zhou Wen finished feeding the mice, he stood quietly aside.
Some of the students complained and started experimenting.
The instruments in the laboratory are the best.
"I'm going to extract plasmid DNA today, Kanazawa Hikaru and you come and help me get a hand. Kim Sung-san ordered.
With a commanding tone.
"Okay, senior. Kanazawa Mitsudo.
Jin Chengshan is an old man in the laboratory, and the newcomers dare not offend, and the relationship between Jin Chengshan and Cui Haoyong is also relatively good.
"You come, go and make LB liquid medium first, LB solid medium, and solution I, solution ......" Jin Chengshan sat on the chair and commanded.
Looks like he's not going to do it.
Seeing Jin Zeguang busy going around alone, Jin Chengshan's face showed a look of sarcasm.
What can I do if I study well, I don't have to work for me.
At this time, Jin Chengshan saw Zhou Wen.
When he saw this guy, he felt unhappy.
Why should a Chinese study in our noble Korean laboratory?
Jin Chengshan, in his heart, looks down on the Chinese.
"What are you still doing in a daze? to help? Are you going to feed the guinea pigs?" Jin Chengshan reprimanded.
gave Jin Zeguang some face, but he didn't save any face for Zhou Wen.
"Okay. Zhou Wen walked over.
Such a cowardly appearance made Jin Chengshan even more disdainful.
"It's rubbish. Jin Chengshan muttered.
Zhou Wen came to Kanazawa's side.
"Ren Chao, don't be angry, the senior is not very good-tempered. Kanazawa Guang comforted.
"It's fine, as long as you can learn something. Zhou Wendao.
LB liquid medium needs to be prepared with several reagents.
Peptone, yeast extract, sodium chloride, after being prepared together, the pH should be adjusted to between 7.2-7.5 with sodium hydroxide, and steam sterilized under high pressure for 20 minutes.
The preparation process is not difficult, but it is more cumbersome.
This kind of low-level work, Jin Chengshan can't look down on it at all.
Zhou Wen and Jin Zeguang quickly prepared all the reagents they needed.
"Zhou Wen, you are very powerful. Kanazawa Guang admired.
There are a total of eleven kinds of reagents needed, Jin Zeguang has only prepared four, and Zhou Wen has already done all the other seven.
"I just look a little bit more. Zhou Wen said modestly.
With his current learning ability, it is not difficult to learn this.
Experiments at this level can be done without opening a virtual training room at all.
"Senior, all the reagents are ready. Kanazawa Mitsudo.
Jin Chengshan got up, put the white coat on his body, absorbed the disinfection, and put on gloves.
"You go and bring my medium. ”
Jin Chengshan looked at Zhou Wen.
"Bacterial culture medium?" Zhou Wen asked.
"That's right. Jin Chengshan said lightly.
"Senior Jin, since you already have a culture medium, why do you need us to make a new one?" Jin Zeguang wondered.
"I'm testing your skills. ”
Kim Sung-san is on his toes.
The medium is used to grow bacteria, and it usually takes 24 hours.
Jin Zeguang thought that Jin Chengshan was planning to cultivate now, but he didn't expect that people would deliberately summon him.
Clay figurines also have three points of fire.
"Senior, aren't you wasting medicine by doing this?" said Kanazawa Guang solemnly.
"I'm not asking you to get it, why are you talking so much, hurry up!" Jin Chengshan said angrily.
Zhou Wen pulled Jin Zeguang and said in a low voice: "It's okay, I'll just go get it." ”
After a while, Zhou Wen brought the culture medium over.
One liquid medium, one solid medium.
It's all cultured strains.
Jin Chengshan was very satisfied and said: "Follow me to study hard, if you behave well, I can consider saying a few words for you in front of Teacher Cui." ”
"Then thank you, Senior Kim. Zhou Wenpi smiled and said with a smile.
Jin Chengshan smiled smugly and put the medium away.
For the extraction of plasmid DNA, the method used is the most commonly used alkaline lysis method.
Kim Sung-san began to operate.
The students next to him began to observe.
There's a lot of equipment used in this.
Centrifuge the culture medium, filter, precipitate, shake, mix, and invert the tube......
Zhou Wen looked a little dozing, these processes were very simple compared to preparing the strengthening elixir.
Even the simplest H-series enhancement potions are more than ten times easier than this process.
Zhou Wen mastered the preparation of the strengthening potion, so he naturally looked down on these.
Kim Sung-san's experimental process is already halfway through.
At this time, Jin Chengshan's face changed suddenly, but he quickly covered it up.
"Hikaru Kanazawa, you can continue the rest of the experiment. "Kim Cheng Shan Road.
"The rest of the experiment isn't very difficult, so I'll leave it to you. ”
Kanazawa nodded and walked up.
However, Zhou Wen sensed that something was wrong.
If there is no problem with this experiment, there will be a maximum of dozens of minutes left to complete it later, but Jin Chengshan asked Jin Zeguang to do it at the moment, and there must be fraud in it.
Kanazawa was simple-minded and didn't notice it, so he took over the experiment and began to do it.
Finally, after dissolving the pellet in TE buffer, Kanazawa took the test tube and put it in the refrigerator.
"It's over. Kanazawa breathed a sigh of relief.
The whole experiment took an hour.
Jin Chengshan smiled and said, "Yes, yes, your skills are getting more and more proficient." ”
Kanazawa smiled and didn't speak.
"Did you ask anything weird?" one student asked.
Jin Zeguang sniffed, frowned, and said, "It seems to have a strange smell!"
The crowd began to search for the source of the flavor, and finally locked in the refrigerator.
The smell came from the refrigerator.
Jin Chengshan immediately rushed over and opened the refrigerator door.
I saw that the test tube in the refrigerator was actually foam inside, and the fine foam was bubbling out, emitting a strange smell of rotten eggs.
"It's not good, clean it up quickly! The solution is broken!" Jin Chengshan shouted.
He covered his mouth and nose and stepped back.
There may be some toxic gas in this smell.
"It can't be chlorine, right?"
Not one of them dared to go up.
"Jin Zeguang, it's all your fault, did you make a mistake in the steps and caused the solution to fail to be prepared?" Jin Chengshan said angrily.
"No way, I'm doing it all according to the norms. Kanazawa was frightened.
The bubble is still bubbling out.
It's just that no one has caught up at all.
"Ren Chao, why are you still stunned?" Jin Chengshan scolded.
"Oh. ”
Zhou Wen walked over, put on gloves, took out the test tube, and poured the liquid inside directly into the special recycling bin.
These smells do not affect him.
He could even do without gloves, but that was a bit of a notice.
"Who told you to dump something?" Jin Chengshan began to curse again.
"This thing is no longer usable, why don't you fall down?" Zhou Wen asked rhetorically.
"This is something that I have spent a few days preparing, will you be able to compensate me if you fall?"
Jin Chengshan is aggressive.
"The solution failed because you set the wrong temperature when you centrifuged after adding the No. 3 solution to pre-cool, so the experiment failed, and you are now putting the responsibility on others, what do you mean?"
Zhou Wen said coldly.
"What nonsense are you talking about?" Jin Chengshan's face changed.
"If it weren't for the fact that you had already found out that it would fail, how would you let Kanazawa Mitsu do the experiment? Don't you just want to put the blame on him? Now you want to put the blame on me?"
Zhou Wen pressed forward step by step.
In the laboratory, everyone's faces changed.
After all, Zhou Wen has always been amiable and angry, and he has never lost his temper at all.