Leaving the affairs of Twilight to someone else, Todd buried himself in the lab's small building.

Before we can officially start building the Successor army, there is one thing that needs to be confirmed first.

This is the body's ability to carry the Sutherland bacteria. In other words, if a large amount of Sutherland bacteria is injected into a normal person's body, will it cause adverse reactions?

The rationale for this question is that the overuse of anything can lead to negative effects, let alone a vehicle like the human body, which requires a high level of precision and balance. For example, the use of ergotamine, triptan, opioids, etc., can lead to headaches, muscle spasms, psychedelic nervopsy, addiction, etc., and the overuse of antibiotics can lead to a decline in immune function in the body and an increase in bacterial resistance, resulting in the emergence of superbugs.

As far as Todd knows, there are only three people who have been artificially injected with Sutherland bacteria by himself. One is himself, one is Huggins, and the other is Edgar. As of today, none of the three have had any adverse reactions. However, the three of them did not inject much of the Sutherland bacteria, Todd injected about 20 milliliters of bacterial liquid, and Huggins and Edgar injected less than 15 milliliters.

Then, the first thing to calculate is the number of bacteria contained in the bacterial solution.

To confirm the number of colonies of bacteria, it is necessary to test for Sutherland bacteria using colony forming units. The specific method is to dilute one milliliter of bacterial samples obtained from volcanic environment culture flasks with sterile normal saline serially, take an appropriate dilution (generally 3 dilutions), inoculate the plate by coating method, and directly count the colonies on the plate after a certain period of culture. This method can determine the number of viable microorganisms that can be cultured in a sample of Sutherland bacteria, so this method is also called "viable bacteria counting" in microbiology.

Todd eventually detected the unit density of Sutherland bacteria in the volcanic environment culture flask at about 170 cfu/ml (note, this refers to the number of colonies, not the number of bacteria), and Todd injected 3400 units (170×20) of colonies of Sutherland bacteria, and Huggins and Edgar injected 2550 (170×15) colonies.

So here's the problem.

Dig...... What is the maximum number of Sutherland colonies in the human body?

Todd designed an experiment on drug tolerance. The general idea is as follows: inject "Sutherland bacteria" into the rats to observe the maximum drug tolerance of the test subjects. Then, according to the tolerance ratio of humans and animals, the bacterial tolerance of the human body is calculated.

What is the tolerance ratio?

The tolerance of humans and animals to the same drug varies greatly. Generally speaking, the tolerance of animals is greater than that of people, that is, the amount of medication per unit body weight, and animals are often several times or even dozens of times that of humans. Therefore, the dosage of human is converted into the dosage of animals, which is generally the following proportion: the dosage of human dosage is assumed to be 1 unit, 25-50 times that of mice and rats, 15-20 times that of rabbits and guinea pigs, and 5-10 times that of dogs and cats.

When the experiment began, Todd selected five mice, all of whom were of similar age and weighed roughly 20 grams. 170 cfu/ml of Sutherland's bacterial solution, diluted tenfold to 17 cfu/ml, injected once a day with a 1 ml dose.

On the 9th day, after 9 injections of bacterial fluid (9 ml in total, 153 Sutherland colonies), the mice developed anorexia, vomiting, and low vitality.

On the 11th day, after 11 injections of bacterial fluid (10 ml in total, 187 Sutherland colonies), the mice developed seizures and convulsions.

On day 15, after 15 injections of bacterial fluid (total 15 ml, 255 Sutherland colonies), the first mouse died after 7 h and the second after 11 h.

After autopsy and pathological tests on the mice, the test subjects mainly died of nerve infections caused by germs.

It seems that the "Sutherland bacteria" is not used at will, and the wanton abuse of super-large doses will cause unpredictable and serious consequences for the host.

Next, we begin to calculate the upper limit of the human tolerability. Rats developed symptoms on day 9 and were injected with 153 Sutherland colonies, which were calculated in terms of tolerability between humans and mice, divided by 37.5 (median interval), which equals approximately 4.

A mouse weighing 20 g (0.02 kg) and an average human man weighing 75 kg with a body weight ratio of 1:3750 would give a Sutherland colony tolerance of 15,000 (4×3,750) for the average man. That is, once there are more than 15,000 Sutherland colonies in the human body, pathological symptoms will appear.

According to Todd's body, 3,400 Sutherland colonies can suppress 3 abilities, so 15,000 Sutherland colonies can suppress 13 abilities, and then make conservative adjustments, so that a 75-kilogram adult man can have about 10 abilities at the same time.

Through this tolerance experiment of Sutherland's bacteria, Todd finally came to the following conclusions:

A 75 kg man was used as a control.

1. The upper limit of the maximum number of Sutherland bacteria that can be tolerated by humans is 15,000 colonies (this level of quantity can already cause pathological symptoms), and if the density of Sutherland bacterial solution is 170cfu/ml, that is, the maximum injection volume is 88.25ml.

2. Once humans are injected with more than 15,000 Sutherland colonies, they will develop neurological infections caused by bacteria, with initial symptoms of anorexia and sluggishness, epilepsy in the middle stage, and neuronal degeneration and death in the late stage.

3. The upper limit of the ability that humans can accommodate is 13, and if it is safe to be safe, it is conservatively estimated to be 10.

All in all, if a human is transformed into an "inheritor", he can carry up to 10 abilities, and if he exceeds this number, he may be sick.

Once you've determined the maximum number of abilities the Inheritor can carry, the next step is to determine what abilities to carry.

Todd made a statistic, and after repeated testing and screening, there are currently a total of 18 valid and usable abilities in the "Ability Library", which are divided into three categories according to the type of ability.

Elemental Attack:

"Flame Breath", "Frozen", "Strong Acid Blood", "Sonic Shock", "Static Field"

Physical Enhancement Department:

"Perspective", "Night Vision", "Eagle Eye", "Pain Resistance", "Regeneration", "Speed", "Skin Breathing", "Hands and Feet Webping"

Deformation mimicry system:

"Metalskin", "Limb Transformation", "Chameleon Camouflage", "Stone Fist", "Blood Marker"

What Todd needs to consider now is which of these 18 abilities to extract and inject into the 13 "heirs" led by Huggins?

The following criteria were selected:

1. Try to choose abilities that are not obvious in their manifestations. After all, too flamboyant abilities will expose the identity of the alien species, which will attract attacks from the church and the world. So, elemental attack abilities can't be chosen.

2. It should be adapted to the way the Knights fought their operations. Considering that most of the terrain around Dusk West is dominated by plains, and the heavy knight's group charge will be the main means of combat, in terms of ability selection, more charge and protection abilities should be selected.

3. Pay attention to the number of abilities and leave space for the later stages. An Inheritor is capped at 10 abilities, but if you reload all 10 abilities now, you won't be able to erase or replace them if a better ability becomes available in the future, as Todd doesn't have any way to remove the xenomorph from her body yet.

After thinking about it for a while, Todd finally selected three xenomorph abilities for the 13 "Inheritors", which are "Regeneration", "Pain Resistance", and "Metalskin".

In addition to this team, Todd also plans to equip them with a scout, Edgar, and in addition to the opponent's existing "speed", he plans to inject the scout with "night vision" and "hawkeye" abilities.

With the above decision in mind, Todd approached the 14 people for a meeting in the side hall of the church, where he presented his plan and explained some of the processes and risks.

Huggins and Edgar weren't worried, and what made him a little embarrassed was the 12 of Huggins' former subordinates. Because these people are not aliens, they are all normal people, and it is reasonable for them to undergo bacterial modification as an order beyond the normal scope.

To Todd's surprise, the 12 sergeants listened to him and said they could receive the injections immediately.

After repeated confirmation by the priest, the sergeants still insisted on their opinion.

Finally, these 14 people were transformed by the bacteria of the "Inheritor" in three days. Except for one sergeant who had a strong rejection reaction when receiving Sutherland bacteria, but fortunately with timely treatment, he was not in serious trouble.

At this point, including 1 (former) knight, 12 (former) sergeants, and 1 (budding) scout, the first combat squad of the Dusk West Knights was officially established.

With this in mind, Todd instructed Huggins to begin recruiting peasants and believers in Twilight. In peacetime, the peasants were mainly responsible for the management and management of the Order's educational property, and in wartime, they were mainly responsible for the transportation of materials, and sometimes they were also responsible for cleaning up the remnants of the army and cleaning the battlefield. The Believers are generally the basic military force of the Church Institution, they are composed of devout seekers, who are usually under the leadership of the Church Knights, who are trained in combat skills and arranged in formations, and in war, they will follow the knights and sergeants as the third echelon of the Order's military force, fighting.

With these things dealt with, the time came to the end of September and the beginning of October in the year 1170 A.D., less than two weeks before the official proclamation of the Council of the Papal States and the Knights of the Twilight.

On the morning of that day, Todd walked the streets of Twilighttown, watching Joe and the monks measure the red line of land and the spacing of the houses, and at the same time discussing the planning of the streets and the layout of the buildings with the craftsmen led by the elder Aiden, Huggins and the sergeants led a group of 68 seekers to conduct basic formation training in the open space not far away, and Alfonso was on the distant hills, holding a big sword and struggling to do physical training.

Todd himself, as agreed, came to the table on the first floor of the hotel on the first day of the opening of Rachel and Karin's "sister hotel", looked at the dazzling dishes on the menu, touched his shriveled stomach, and thought about what to eat, when a figure stumbled in at the door.

Todd looked up and saw that it was a new scout with a panicked face, Edgar.

"Cavalry! A lot of cavalry is coming up to town!"

Hearing his words, Todd hurriedly put down the menu in his hand, stepped out of the store, walked to the square, and looked into the distance along the street. Using the ability of Eagle Eye, Todd clearly saw more than a dozen heavily armed knights with silver rings on the plains, rushing towards Twilight Town.

When Todd saw this scene, the first thought in his mind was - are these people crazy?

If the armies of the secular kingdom invade the territory of the Church without permission, will anyone be arrogant enough to be the enemy of the entire Church?

It wasn't until the group got closer that Todd saw something strange. The old knight at the head had white hair and was covered in blood, and there were a few arrows stuck in his armor, and it looked like it was Baron Cumberland, and there was a man sitting behind him. Todd tried to tell that the man seemed to be a young woman, dressed in a lavish court dress.

When Todd saw who the woman was, he couldn't help but gasp.

Queen Mary......