At 12:15 in the middle of the night,

There was still an hour before takeoff, Xu Mang sat in the VIP waiting hall, his eyebrows revealed a trace of anxiety, he received a call from Academician Zhong before, and was told that his AI program had a very serious error, and the experimental data and AI data were different in several places.

"That's not right..."

"Why is this happening?" Xu Mang looked at the content on the laptop screen, his expression was very confused, the data on both sides did not match, this is very strange, it is impossible to do this under normal circumstances, I have never encountered it.

Don't...

Dude is capsizing in the gutter?

In the face of this kind of thing that may happen, Xu Mang's self-esteem is unacceptable, he has always been used to success, but suddenly facing failure, let alone ... I don't know what to do, panicked, overwhelmed, confused?

In a word... At this moment, Xu Mang's heart is mixed, and he can't say a kind of discomfort.

If there is a problem with one experimental data, forget it, all seven data have problems, which is very illustrative, it must be a mistake, Xu Man No. 2 artificial intelligence system seems to be tragic, either to overturn and restart, or to develop No. 3.

"Professor Xu?"

"Your coffee... Pay attention to the hot mouth. A beautiful and generous flight attendant brought a cup of coffee to Xu Mang, in fact, this flight attendant was not supposed to serve Xu Mang, but the big boss of Modu personally called the chairman of Modu International Airport and made sure to take care of Xu Mang, which made a matter of a flight attendant serving Xu Mang alone.

"Thank you. ”

Xu Mang replied absentmindedly, looking at the data on the screen, completely ignoring the flight attendant next to him, and then the mobile phone placed on the side rang, and the caller was Yang Xiaoman.

"Hello?"

"Still not sleeping so late?" Xu Mang picked up the phone and asked curiously: "It's easy to get wrinkles when you sleep too late, I was still a beautiful girl, but after a few days, I became a yellow-faced woman, although I pursue spiritual beauty, but the premise is that the other party's appearance must meet the standard." ”

“...”

"Stupid... I'm worried about you, and I can't sleep a little. Yang Xiaoman said glutinously: "What trouble did you encounter? This is about to celebrate the New Year, and let you go to the capital... I don't know how to be considerate of others at all, and I was happy to celebrate the New Year, but now I have added a lot of blockages. ”

In the face of Xiao Man's chattering complaints, Xu Mang just smiled and said indifferently: "This is the norm... Actually, I'm already very free, and there are a lot of scientific researchers you don't know or know at all, and they work harder than me... Be willing to be an incognito celebrity and achieve earth-shattering things. ”

"Xiao Man..."

"This year's Chinese New Year... I may not be able to come back, don't forget to come to my house in the thirtieth year of the Chinese New Year's Eve, my parents miss you very much. Xu Mang said helplessly: "I have encountered a problem that cannot be solved for the time being. ”

Yang Xiaoman was silent for a long time, and said questionedly: "I know... Don't worry about things at home, I'll take care of everything. ”

afterwards

The two whispered something and didn't hang up until fifteen minutes before takeoff.

...

got off the plane,

Xu Mang saw the staff who had been waiting for a long time.

"Good work. ”

"Wait for me on a cold day. Xu Mang smiled bitterly: "Anyway, there are still people in the research institute now, there should be no one at four o'clock in the morning, right?"

"Hmm!"

"Professor Xu, shall I take you to the guest house first?" the staff member asked.

Xu Mang thought for a moment and said seriously: "You send my institute first, I can't sleep for the time being, by the way... The janitor should have the key to the lab, right?"

“...”

"Professor Xu, why don't you go back to the guest house first, Academician Zhong asked me to send you to the guest house. The staff member smiled bitterly: "You just got off the plane, let's take a rest first, tomorrow... You might be a little busy tomorrow. ”

"Oh..."

soon

Xu Mang went to the so-called guest house, in fact, it was the place he came to last time, it may be the reason for the upcoming New Year, Xu Mang quickly moved in, came to his room, did not feel sleepy at all, took out the notebook from his handbag, and continued to look at the data.

Logically speaking,

Even if it is not an upgraded CRISPR-Cas9 system, it should have a high target accuracy, but why is the upgraded CRISPR-Cas9 system a 100% error rate?

"Ugh..."

"I'm going to die!" Xu Mang lay on the bed, looking at the ceiling above his head, and the emotions that he couldn't let go of for a while gradually came to his heart, he really had no clue, he originally thought he had won, but he never expected that this was just opening Pandora.

Unconsciously,

Xu Mang fell asleep until four hours later, when he was woken up by a phone call.

"Hello?"

"Hmm... I'll come right over. Xu Mang gave a hitch, washed up, and then went to the Institute of Biochemistry and Cell Biology of the Academy of Sciences.

soon

Xu Mang met Academician Zhong, the head of the Institute of Biochemistry and Cell Biology of the Academy of Sciences, but his state was very poor, his eyes were faintly bloodshot, it seemed that he didn't sleep well yesterday, thinking about it... Out of this situation, how can you sleep well.

"Professor Xu Mang. ”

"I've finally waited for you. Academician Zhong smiled bitterly: "This gap is too big, almost all your AI calculation results are wrong, and it is a very serious off-target error, Professor Xu Mang... What the hell is going on?"

Xu Mang shook his head and said helplessly: "I don't know either, so I hurriedly came over to find out the situation." ”

Then

Academician Chu walked in, was stunned for a moment when he saw Xu Mang, and hurriedly asked, "Professor Xu Mang?"

"This is Lao Chu, Academician Chu. Academician Zhong introduced.

"Hello Academician Chu. ”

After a simple greeting, the three of them began to talk about the root of the problem, but after ten minutes or so, they still had no clue, but the most embarrassing one was Xu Mang, after all, the problem was all on him.

Immediately afterwards, Xu Mang came to the laboratory, got a more detailed information report, and then applied to Academician Zhong for a temporary office.

"Professor Xu Mang?"

"Did you find any problems?" asked Academician Zhong.

"Not yet. Xu Mang still shook his head, and said helplessly: "But Academician Zhong can rest assured... Since I promised to help you upgrade your CRISPR-Cas9 system, I'll do it, right... Can you give me a key to this office?"

"Huh?"

"Professor Xu Mang?"

"Aren't you going back?" asked Academician Zhong.

"Hmm!"

"My pride won't let me go back. Xu Mang said seriously: "This matter can't be solved, I feel very uncomfortable all over." ”

Time

Academician Zhong didn't know what to say, he had listened to his old friend, Xu Mang's second grandfather before, this guy's head is not ordinary iron, and he can't pull back ten cows when he is stubborn, he originally thought that it might be mixed with some water, who would have thought... It's all true.

"Professor Xu Mang. ”

"I see that the Chinese New Year is coming, so let's do it again after the New Year. Academician Zhong said.

"Forget it..."

"It's better to lock yourself up at home, and it's better to be here than to go back with your worries. Xu Mang smiled bitterly: "It's better to be alone than to be alone, but it's better to be alone than to be alone, Academician Zhong... Tell the researchers in the institute to come back for the New Year... I'll give them a satisfactory answer, so let them relax. ”

Hearing Xu Mang's words,

Academician Zhong knew that he was going to take all the responsibility and resist it alone.

...

There are still three days left before the Chinese New Year,

The Institute of Biochemistry and Cell Biology of the Academy of Sciences is already empty, and almost all of them have gone home, except for those who have been arranged to be on duty.

In these days,

Xu Mang was not idle, he found the problem, the upgraded CRISPR system, should not be edited by Cas9 nuclease, a smaller nuclease is needed to complete the task of cutting off 1 DNA strand, and then the reverse enzyme is guided by the RNA strand to perform gene editing at the cut.

At this point,

To find a new pair of genetic scissors, the new RNA-directed nuclease needs to have three characteristics: smaller, more stable, and more complete.

Smaller means easier use for clinical gene therapies that require in vivo delivery, more stable because it can maintain high enzyme activity over a wide range of temperatures and pH, and safer because it has less off-target effects.

But...

It's not ordinary!

In fact

Xu has two new RNA-directed nucleases, Cpf1 and C2c1, which are smaller than Cas9 and easier to enter tissues and cells, especially Cpf1, which is cleaved far from the recognition site, allowing researchers to have more choices in the choice of editing sites.

However, Xu Mang prefers C2c1, an RNA-oriented nuclease, which is smaller than Cpf1 and easier to achieve cell-to-cell delivery through viral vectors, but C2c1 has the characteristics of thermophilic properties, which is difficult to develop into a good gene editing tool.

Can't

C2c1 comes from acid-resistant and heat-resistant bacteria, which requires a certain temperature to operate normally, according to a foreign research report, its optimal temperature is 48 degrees Celsius, which can burn ordinary mammals to high fever directly.

Redesigning the C2c1 temperature is a challenge for Xu Mang, and as long as this challenge is completed, all the next problems will be solved.

Then

The phone rang,

The caller is an uncle.

"Hello?"

"Uncle..."Xu Mang said bitterly, "Yes... I'm in the capital, and I'm not going back for the New Year, oh... I'll take care of myself, don't worry... Hmmm!"

hang up

Xu Mang continued to pounce on the process of solving the problem, but only ten minutes later, the phone rang again, this time it was Xiao Man calling.

"Hello?"

"Husband... I got five catties of superb tea for my dad and bought a necklace of more than three million yuan for my mom, it should be about the same, right?" Yang Xiaoman asked: "Speaking of your relatives and friends, do you want to bring it too?"

"Uh..."

"Buy some gifts for my brother and sister. Xu Mang said indifferently: "For example, "5-year college entrance examination 3-year simulation", "5-year high school entrance examination 3-year simulation", "college entrance examination must be brushed"... Let these little ones not want to have a good year. ”

“...”

"Why are you so bad?" Yang Xiaoman said angrily: "I'm also the sister of these people, forget it... I won't tell you, work hard, I'll go shopping again. ”

syllable

Hung up,

Xu Mang put down his mobile phone, looked at the various materials on the table, and wanted to cry without tears for a while.

Specially,

Labor and management fight with you!

......