Lan Jie carefully extracted the plasmids of the given bacteria, and he measured the concentrations of the extracted plasmids to be 87.5ug/ml and 92.5ug/ml.

Bang!

The player in front of him accidentally broke the test tube, and the reagent liquid flowed on the ground.

"Teacher...... Right...... I'm sorry...... I...... I ......" this player is stupid, he is frightened and incoherent.

"This is the new reagent, hurry up. "The invigilator is reasonable.

"Thank you, teacher!" the contestant immediately redid the experiment.

Points will be deducted for scrapping experimental equipment and reagents, and sure enough, some players have given them a chance.

Ranger continued to do his experiments, and he moved on to electrophoresis.

The electrophoresis time was 1 hour, and while waiting for the electrophoresis, Lanjie began to answer the questions.

There are a few questions on the experiment log paper:

1. Please briefly explain the role of topoisomerase in DNA replication and transcription.

2. What is the difference between the mechanism of action of topoisomerase I and topoisomerase II?

3. What is the principle of topoisomerase inhibitors as tumor inhibitors?

After answering these three questions, please read an article on the mechanisms of human immunity and continue to answer the following questions:

4. Which topoisomerase I activity is higher in common B cells or central B cells?

5. If topoisomerase I mutations occur in B lymphocytes, what diseases are most likely to cause?

......

The intensity of this experimental competition is very high, and the contestants not only have to design experiments, practice by themselves, but also solve theoretical problems in the process of experiments.

Lan Jie finished all the theoretical questions 5 minutes before the electrophoresis was completed, and he quickly checked it before continuing the experiment.

"Teacher, my experiment is done, please help me print the results of the experiment. Ranger raised his hand and offered his needs.

"Wait a minute. The invigilator immediately printed out Lan Jie's experimental results and handed them to Lan Jie for verification.

Rangie carefully examined the results of his experiment, and found that the first lane was not treated with topoisomerase I and had two bands, the second lane was treated with topoisomerase I, and many bands appeared between the original two bands, and the third lane was treated with enzymes for a longer time, and the lower band was lighter and the upper one was lighter......

There's nothing wrong with that.

Even if there is a problem, there is no time to redo the experiment.

Based on the results of the experiment, Ranger perfected his experiment record paper.

Inspect and check, Lan Jie checked until the last 5 minutes of the experimental competition, then handed over the paper, handed over the drawings, cleaned the test table, and left.

The total score of the national finals and the list of winners will be announced the day after tomorrow, and the contestants will have one and a half days to play freely.

Lei Tiangang said that the students have worked hard, the competition has ended, no matter what your results are, there is no other arrangement for the next day and a half, just play, I hope the students have fun in the big magic capital.

Each coach of the Yang Zhongsheng Competition Team led a wave of players to check in at various attractions in the magic capital, and a day and a half passed quickly.

It's finally time for the results to be announced.

Early in the morning, all the members of the sheep competition team gathered and waited for the organizer to announce the scores and winners.

Wait, wait, wait, wait until noon, and the organizer will release the list.

National One Award, 71 people, ranked as follows:

1. Lan Jie (202XXXXX), 199 points;

2. Zhou Moumou (202XXXXX), 197 points;

2. Xu Moumou (202XXXXX), 197 points;

......

71, Zheng Moumou (202XXXXX), 147 points;

"Another point was deducted...... "It's weird this time, I heard that the experimental competition almost didn't get a full score, and Lan Jie's experimental competition got a full score." The 1 point deducted by Lan Jie came from the theory competition, and there were several players who scored full marks in the theory competition, and Zhou Moumou and Xu Moumou, who were tied for second, had full marks in the theory competition.

The sophomore student of the Yang Zhongsheng competition team was amazed: "Senior Lan Jie is awesome! 199 points, the No. 1 national prize winner! You are the only student in our sheep and even the entire Dayue Province to compete for the national first prize!"

"Hahaha, it's okay. "Although one point was deducted, Ranger was still happy.

There are 71 National First Awards, 78 National Second Awards, and 185 National Third Awards. The remaining contestants did not receive awards.

The sophomore in high school only scored 45 points in the theory competition, and he got a high score of 97 points in the experimental competition.

With a total score of 142, the sophomore was only 5 points away from the national line, and he won the national second award.

"It's a pity for the junior brother, if you get a few more points in the theory competition, it will be the first prize of the country. Lan Jie admires this junior, this junior's experimental ability is extremely strong, and the junior almost turned the tables.

"Hey, yes, the pit dug in the theory competition is too deep, but it doesn't matter, it's not bad to get the second national award, I still have a chance next year." The junior brother thought about it, and he said, "Brother Jie...... Oh no, Daddy Jie! You're a dad now! There are two champions in the college entrance examination in our province every year, one in liberal arts and one in science, and there is only one contestant in the first prize of the country, and that is Daddy Jie! And Daddy Jie is still the No. 1 winner of the National First Prize, and he is the champion among the first prize winners!"

The happiest person was, of course, Lei Tiangang, who shouted again and again: "Congratulations!"

This was followed by the award ceremony, which was reported by some media outlets.

This year's 71 national awards are an average of 2.00 per province.

However, the actual situation cannot be simply divided and averaged.

Hunan, Jiangsu, Zhejiang, Shandong, Hebei, and Hubei provinces have won 60% of the national awards.

After the award ceremony, two forces acted at the same time.

One force is the Shengjing National Team, and they immediately sent out invitations to the top 50 National One Prize winners, hoping that the top 50 will join the Shengjing National Training Team to prepare for next summer's IBOs.

The other force should be called the power group, and this large group is composed of small groups such as Shuimu, Yanda, Modu Jiaotong University, and Fudan University.

These prestigious schools with strong biology majors have only one goal: to grab people and compete for the first prize in the national competition.

The biological strength of Modu Jiaotong University, especially the strength of bioengineering, is very strong, and they cooperated with the Chinese Biological Association to hold the national decision of this biological competition.

Modu Jiaotong University is also the training base of this year's Shengjing National Training Team, Lan Jie won the Shengjing National First Award with the first overall score, he temporarily stayed in Modu and did not return to Aries City.

A person in charge of the Chinese Biological Association surnamed Ma found Lan Jie and Lei Tiangang.

Mr. Ma did his homework, and he said, "Lan Jie, congratulations! I also learned that you were sent to Mizuki University before you participated in the national competition. The next thing is very simple, Lan Jie, welcome to the national team!"

Lan Jie accepted Mr. Ma's invitation: "Well, good!"

Teacher Ma said again: "Lan Jie, Mr. Lei, you can stay at Jiaotong University all the time, or you can return to Aries City first." The coaching team and logistics team of this year's student competition national team are expected to be assembled in place in ten days, so please wait for the notice. ”