Chapter 81: Archaeal Bacteria Breeding
The technique of "grafting" is not Todd's major major, but a skill he mastered in the interest elective courses he chose in college in his previous life. When he chose that course, he naturally didn't want to study, but was completely a beautiful female teacher who was an elective course. Unexpectedly, when traveling to another world, this craft came in handy.
When all the materials were in place and the "Moon-Season Grafting" could begin, Todd first needed to do some preparations.
One is disinfection. Disinfect knives and palms with 75% alcohol, and disinfect wooden clips and bundles with formaldehyde solution in ventilated areas.
The second is to choose "scion", try to choose no pests and diseases, no nodules, one year or less than a year of monthly branch buds.
The third is to deal with rootstocks. The branches and leaves of the wild rose and the upper part of the stem are cut off, leaving the roots and the lower part of the stem, and the cut is a smooth cross-section.
The next step is the formal grafting work.
Todd first made a horizontal cut at the rootstock 6 cm from the soil, about 8 mm wide, just enough to penetrate the bark to reach the trunk, and then made a vertical cut under the middle of the cross-cut, about 2 cm long. This leaves a T-shaped incision on the bark of the wild rose.
Then select a suitable spike among the moonflowers, remove the leaves and leave the petioles, choose a full bud, and use a knife to cut an incision diagonally on the branch.
Finally, use a knife to pick open the cortex of the T-shaped incision of the rootstock, and carefully insert the sprouts into the incision. Immediately after placing it, it is secured with a wooden clip and tied with a tie rope.
Repeat the above process, and according to the viability and vitality of the rootstock, up to a dozen scion can be inserted. You can choose a few more colors and styles to enhance the beauty and styling.
The grafting is carried out here, which is equivalent to half the success. Todd kept introducing the attention points of each link to the farmers around him.
Next, it's the turn of how to nurture and maintain the finished grafted product.
The first is temperature and insolation. The temperature should be kept in a relatively stable range, preferably between 22 and 30 degrees. And it is best to ensure more than half a day of sunshine every day. So, Todd instructed the farmers to keep the grafted moon in the warehouse where the fire was raised at night, and move it outdoors during the day when the sun was shining.
This is followed by pruning and fertilization. In the process of growing in the moon season, it is necessary to cut off the lateral branches, diseased branches and concentric branches in time to prevent nutrient diversion. As for fertilization, once every 10 days, the fertilizer is a little broad bean shell, bean cake or chicken and pigeon manure, etc., which can make the moon season continuously absorb various nutrients such as nitrogen, phosphorus and potassium from the soil.
Finally, and a crucial trick: preservatives. A mixture of low concentrations of silver thiosulfate and aluminum sulfate is poured into the soil to prolong the flowering period and increase the brightness and delicacy of the flowers.
To sum up, the success of "moon and season grafting" mainly has the following points: disinfection, incision, scion, temperature, sunshine, pruning, fertilizer and preservation. The whole process seems simple, but as long as any link is wrong or not in place, it may cause the entire grafting work to fail completely.
Todd stayed in the warehouse for only two days, and he taught the farmers all the processes and steps of grafting, but did not give the recipes for disinfectants, fertilizers, and preservatives, but kept enough finished products, claiming that these were blessings from the Father.
Leave the rest of the work to the farmers, and order the guards of Twilight Town to guard the warehouse 24 hours a day, and no one will be allowed to approach. Todd couldn't wait to get back to the lab, as he had more important things to do.
Huggins told him that the dish for Sutherland archaea was finished.
Artificially reproducing "Sutherland archaea" is related to his net worth, so he can't miss it at all, and other things can naturally be put back.
Walking through the door of the lab, Todd finally saw the petri dish he had been dreaming about. Rather than a petri dish, it might be more accurate to call it a "culture oven".
The shape of the cylinder, made of copper alloy, is more than one meter high, and the internal structure is divided into four layers. The upper layer is a gas pipeline, with a gas valve on each side to control the flow and pressure, the second layer is a liquid pipe, which can inject solvent downwards and divert the solution, the third layer is the incubation room, which is used to place the samples or materials that need to be cultured and reacted, and two layers of small glass are specially added here so that Todd can use "perspective" and "eagle eye" to check the internal state, and the bottom layer is a fuel tank, which is used to add flammable products such as charcoal to heat the entire tank.
It has to be said that the craftsman Huggins found has never disappointed since the beginning of refining silver, and the craftsmanship of hand-crafting utensils is really amazing. This kind of large-scale experimental equipment should take into account many factors such as sealing, diversion, heat and corrosion. Originally, Todd didn't have much expectation for the finished product of the craftsman, and planned to make repairs and improvements on this basis, but now it seems that there is no need for that at all, and this "cultivation furnace" can be put into use immediately.
However, before officially starting the artificial culture of Sutherland archaea, it is necessary to extract enough samples from the body.
Todd changed the original syringe into a blood drawer, and then purified, deimpurized, concentrated, and distilled the extracted blood, resulting in a small ember similar to ashes.
Then put the ash powder in the "cultivation oven", put it in the charcoal and start to heat it slowly.
According to the Sutherland Historical Biography, the place where the archaea were found was probably in the crater, so several environmental factors may have been key to the reproduction of the Sutherland archaea.
Temperature, oxygen, and acids and bases.
It's like a key has three teeth, and you have to adjust the shape and size of each tooth to perfectly insert the hole groove and open the door lock.
Based on the volcanic environment, it can only be roughly speculated that the breeding environment of Sutherland archaea is anaerobic and acidic at temperatures above 100 degrees Celsius.
All Todd has to do now is determine how much temperature he needs to reach, how anaerobic he needs to be (microaerobic, facultative anaerobic, or anaerobic), and how much acidic pH he needs to be.
This process of determination is long and uninteresting, requiring repeated experiments and observations, as well as recording and comparing experimental data, while taking into account every factor that can distort the results.
Finally, in the early morning of the nineteenth day after the start of the experiment. Fifteen minutes after Plan 183 was carried out, the "Sutherland archaea" in the culture chamber was like a snowball, condensed into a small ball and slowly rotated in the high temperature, and rolled bigger and bigger.
Todd wiped his red eyes and pressed his face against the glass of the observation hole, with only one thought in his mind.
The artificial propagation of "Sutherland archaea" has been successful!