"Cut the human serum protein acetate membrane into small strips 2 cm wide and 8 cm long, Zhang Kai, you will cut it, and I will debug the electrophoresis equipment. Xia Lu said as he adjusted the equipment.

Zhang Kai cut the fiber film and asked, "When you choose a tutor in your sophomore year, who will you choose in Xia Lu?"

Xia Lu carefully set the electrophoresis parameters and said, "This has to be determined according to the professional direction. ”

Zhang Kai asked again: "Physics, chemistry, biology, which discipline do you choose to major in?"

Xia Lu thought for a while and said: "Biochemistry is an experimental science, and it has strong application. From the perspective of basic theory, physics is relatively high-end. Let's talk about it in my sophomore year, after all, I haven't even finished the first semester of my freshman year. ”

Zhang Kai whispered in Xia Lu's ear: "My goal is very clear, I don't want to do theoretical research, I definitely choose the major direction of application." It is very important to choose a supervisor, and the research direction of the supervisor determines our professional positioning. SO LAO CAO MUST BE PASSED BY ME, HE IS A THEORETICAL PHYSICIST. I'll tell you Xia Lu, don't be fooled by Lao Cao's sugar-coated cannonballs, he is very good to you now, and gave you many pentagrams, that's because he wants to pull you into his physical pit. ”

"Oh, such a scheming?" Xia Lu smiled, unmoved.

"This Professor Xu is very good, he takes the biology class of the Hongyi Academy, and at the same time is in charge of some important biotechnology projects in the School of Biological Sciences. Professor Xu is a biotechnology expert, and any expert with the words "technology" and "engineering" must be an application expert. I want to mess with Professor Xu. Zhang Kai looked at Professor Xu not far away, revealing a longing look.

"Bastard, bastard, you're big! The fiber film is cut into this kind of forced shape, are you embarrassed to follow Professor Xu?" Xia Lu suddenly went crazy, he looked at Zhang Kaiche's fiber film strip, and was angry: "Zhang Kai, are you a pig?

"Oh, I'm going, I didn't cut it well! My fault my fault, I patronized and chatted with you, and there was a bit of a mistake in the operation. Zhang Kai apologized and re-cut the fiber film.

With such a basic and simple biological experiment, Xia Lu never wants to go wrong, not only can't he make mistakes, but he also wants to get a full score in the biology experiment class.

Xia Lu and Zhang Kai completed the experimental operations such as soaking, spotting, and upper groove together, and then entered the electrophoresis stage.

The main experimental operation was undertaken by Xia Lu, and Zhang Kai cooperated with Xia Lu, and the experiments of the two went more and more smoothly.

The electrophoresis voltage is set to 120V, and the electrophoresis time takes 40 minutes.

These 40 minutes are pure waiting time, Zhang Kai flipped through the biological experiment operation manual to strengthen the basic knowledge of the experiment.

Xia Lu is well aware of the operation process of serum protein acetate thin film electrophoresis experiments, and he brought a copy of "Biochemistry", using the 40-minute electrophoresis waiting time to further understand the interdisciplinary knowledge of "Biochemistry".

Professor Xu walked back and forth in the laboratory, observing the students' experimental operations and progress, and chatting with the students from time to time.

"The serum must be evenly dispensed during dispensing, otherwise the electrophoretic profile will be uneven. Zheng Quan, do you really know how to use a spotter? So I just talked about it in vain? Have you carefully observed my experimental operation demonstration?" Professor Xu was very dissatisfied with the experimental operation of a certain group of students, and he criticized in a stern tone: "You re-cut, soak, and count for me, and tell me before I go to the slot, and I can only go to the slot after I confirm that it is OK." ”

The two students were embarrassed, so they started the first step and re-experimented.

"Zheng Quan's group is finished...... Zhang Kai looked at the two classmates who were criticized by Professor Xu, they all talked about the face, and no one wanted to lose face.

"Do your own experiments, don't look around. Xia Lu reminded, he looked left and right, it seemed that he and Zhang Kai's experiment was progressing the fastest.

"Xia Lu, do you know, the thing that men are most afraid of is that it is too fast. Zhang Kai began to prepare the Lichun red dyeing solution, and the cooperation between him and Xia Lu tended to be tacit.

Xia Lu used a ratio of 3 to 97 to mix glacial acetic acid and water to prepare rinsing liquid: "The membrane is a very fragile thing, and when we dye and rinse the membrane later, we must be very careful not to break the membrane." ”

Zhang Kai nodded and said: "Xia Lu, your hands-on ability is strong, the operation of the membrane is handed over to you, and I will draw the electropherotic map." ”

Unconsciously, at the end of the 40-minute electrophoresis, Xia Lu turned off the power, took out the membrane, and directly soaked it in the Ponceau red staining solution prepared by Zhang Kai.

The most basic biological experiments are not difficult, fully understand the experimental procedures and operation details, have a meticulous heart, a pair of dexterous hands, and a teammate who is not too stupid, you can complete the experiment well.

After soaking, Xia Lu took out the membrane and continued to dip and decolorize with rinsing solution until the background was completely colorless.

After rinsing, the membrane clamp is blotted dry in the filter paper, and the next work is handed over to Zhang Kai, who observes the electrophoresis zone and quickly draws the electrophoresis map.

At this time, Professor Xu came to Xia Lu and Zhang Kai's experimental bench to see the state of the membrane, and then looked at the electropherogram drawn by Zhang Kai.

"Yes, the data is good. Professor Xu praised Xia Lu and Zhang Kai.

No matter how tedious the process of biological experiments is, the key evaluation index to evaluate whether an experiment is OK is the experimental results.

Of course, some students make mistakes during the experiment, and Professor Xu can immediately judge that their experimental results must be bad and need to start the experiment again.

Bad operation, good result, nine times out of ten is fraud.

Xia Lu and Zhang Kai's electrophoresis data are quite OK, and Professor Xu did not hesitate to praise him.

"The main reason is that Professor Xu has taught us well, which has benefited us a lot. Zhang Kai is very happy, he is determined to major in biology, and in the future, he can eat with biotechnology experts like Professor Xu.

Professor Xu looked at Xia Lu and asked, "According to my observation, most of the operations in this experiment were completed by Xia Lu, right?"

Professor Xu watched and listened to all directions, and there were more than 20 students in the laboratory, and their every move was under Professor Xu's watchful eye.

Zhang Kai was stunned, and then said: "Yes, yes, Xia Lu's hands-on ability is extremely strong, meticulous, accurate, and 0 mistakes." ”

Xia Lu blew a wave of each other: "Zhang Kai's data processing level is the first in the class, and he is a perfect score in mathematics in the college entrance examination." ”

"In this way, you two are really a golden pair, and you have got the best experimental results in the shortest possible time. Professor Xu smiled and said, "This experiment is not completely done yet, so you can continue to finish the finishing work." ”

Zhang Kai looked at Xia Lu, and left the finishing work to Xia Lu to deal with.

In order to better preserve the specimen, he carefully and meticulously blotted the acetate membrane with filter paper to dry the residual liquid, and then put it into the transparent solution to soak.

Professor Xu stood by with his arms crossed, observing every detail of Xia Lu's operation.