Chapter 23 Xu Wu's Experiments in Progress 2
In this transformation, Xu Wu set several phased goals.
The first is the hair color makeover that has just been achieved.
The second is to make the rabbit's feces not smelly.
The third is to adjust the body aroma of the rabbit and make it more attractive.
The fourth is the ultimate goal, to adjust the brain capacity of rabbits, so that rabbits have the wisdom of 5 to 6-year-old children.
It's just that even if it's just the second goal, it has already made Xu Wu a little big.
This starts with why the feces stink.
Fecal is a product of the digestive system that processes food, and it is odorous mainly due to the presence of indole and hydrogen sulfide in the metabolic process.
Indole, which is produced by tryptophan deacidification or tryptophan oxidative deamination in the human body, is not clear to rabbits, but there should be such a reaction.
The origin of hydrogen sulfide is mainly due to the catabolism of proteins in the body.
How can we reduce the amount of these two in the excreta? reduce the source, reduce the formation reaction, and add the reaction to convert indole and hydrogen sulfide into other substances.
These three ways are Xu Wu's priority. The problem is that the first method is basically not implemented, and it is impossible for rabbits to take no protein. You can't let rabbits eat only glucose every day, right?
If you reduce the production reaction, you can try one or two, but I don't know if the generation pathway in the human body is suitable for rabbits. If applicable, what happens if the reaction in the rabbit is free of intermediates directly through genetic regulation?
Speaking of which, the easiest thing to do is to convert indole and hydrogen sulfide into other substances in rabbits. If the concentration of indole can be reduced to a very low level, there will be an aroma. How do you think about it?
Yes, indole will have a fragrance at very low concentrations, and many perfumes have indole ingredients in it. Although indole will have a foul odor when the concentration exceeds 1%, in nature, the content of indole in the floral composition of jasmine is as high as 5%-18%. This appears to be a combination of chemicals that evolved by natural selection. Unfortunately, the exact ingredients are not clear.
So, let's start by reducing the two main odor components in feces.
Xu Wu began a plan to transform rabbit droppings.
"Implantation of E. coli species in rabbit intestines. ”
"Adaptation of E. coli species to knock out genes that synthesize enzymes that mediate the breakdown of tryptophan to indole. Increase the expression of tryptophan synthase in Escherichia coli. (Indole is a reactant in the synthesis pathway of tryptophan)"
He didn't think of a better way to deal with hydrogen sulfide, after all, he didn't even find the biochemical reaction process of hydrogen sulfide. In terms of chemical properties, hydrogen sulfide has a good reducing ability and can react with substances with relatively high oxidation.
The first thing Xu Wu thought of was oxygen. However, a large part of the intestine is in micro-oxygen or anaerobic conditions. If he forcibly modifies it, it will only make the rabbit's intestinal microbes unbalanced, and the number of aerobic bacteria will increase significantly. The decrease in anaerobic respiration will directly lead to a decrease in intestinal lactate content, an increase in PH, and the loss of optimal pH value in the intestinal digestive enzymes, which in turn will lead to indigestion, bloating, stones, and malnutrition. I really think about it, and the consequences are serious.
"Okay, let's try to see how the rabbit grows after this treatment. Xu Wu lifted the adjustment interface, and the rabbit that landed on the ground didn't seem to have changed.
It was only 5 minutes later that Xu Wu's face changed.
The rabbit he experimented with, it seems that he can't sleep for a long time, and his spirit is getting more and more excited, but his appetite is not high, and in the long run, the growth and development of this rabbit is obviously much worse than that of rabbits in the same period.
It completely declared the failure of Xu Wu's first experiment.
"How so. Too much tryptophan has accumulated in the body?" Xu Wu muttered to himself with a dark face, and clicked on a rabbit again to try.
Try to directly change the biochemical metabolism process in rabbits, increase the activity of tryptophan in other metabolisms, and reduce the concentration of tryptophan in the body. As a result, the situation is even more serious.
After sacrificing more than a dozen rabbits, Xu Wu no longer dared to touch these substances that involved multiple biochemical reactions. In particular, the basic ingredients such as protein, sugar, and fat are really not touched at all. If any reaction is exceeded, as soon as the biochemical balance in the body is out of balance, a large number of chain reactions will immediately occur. I can't adjust it at all.
Xu Wu, who was forced to abandon his plan to improve his feces, set his sights on the next goal - to make rabbits smelly.
Body fragrance transformation, he already has a draft. For this renovation, he specially planted some sandalwood trees, jasmine trees, agarwood trees, and various flowers on the island.
These are naturally planted in order to collect genes and obtain metabolic genes for various fragrance components. What made Xu Wu super sober was that in the information of the gene pool, the metabolic genes of various fragrance components and the color control genes were the same, and there were more detailed explanations, so that he would not be confused like his previous attempts.
"The target, the grass rabbit, was to put plasmids embedded with sandalwood scent chemical synthesis genes in epithelial cells. Xu Wu, who has suffered a loss, no longer puts genes into chromosomes for expression this time, but chooses a method that has less impact on the body and puts genetic material in the cytoplasm.
Plasmids are injected into the cytoplasm to directly use the "translation" system in the cell to produce chemicals about sandalwood, which are then transported out of the cell or accumulated in the cell to form an aroma.
Now Xu Wu is worried about only two questions, one is whether the chemical accumulation of sandalwood will have a negative impact on the rabbit's cells. Second, even if this chemical does not have much negative effect, will it not be transmitted outside the body, so that there is no aroma?
But everything needs to be tried.
It has been said that practice is the only criterion for testing truth.
Therefore, after Xu Wu finished the operation, he observed the living conditions of this new rabbit again.
At first, there was nothing unusual about the rabbit, at least not when he observed it.
There was no mention of high spirits, no loss of appetite, no unbearable pain, and no abnormal growth.
Feeling endocrine, blood circulation, cranial nerves, etc. are normal.
In this way, whether it is feasible or not, at least it does not endanger the health of rabbits.
But soon, the rabbit, who had high expectations from Xu Wu, soon died.
It was bitten to death, and this time it was a feral cat that killed it.
To Xu Wu's disbelief, after the cat bit the rabbit to death, it actually sniffed the rabbit's body, and then walked away in disgust.
Yes, I walked away in disgust.
"Phantom Moon, did you say that it was because this rabbit was too fragrant, exposed itself, and then was caught?" Xu Wu said his guess, and felt that it should be very close to the truth.
Phantom Moon shook her head and said, "I don't think so, and by now, there has been an obvious problem with the rabbit you transformed, the island master. ”
Xu Wu hurriedly asked: "What is it?" I can't see anything at all when I look at this high-speed projection. ”
Phantom Moon pointed to the enlarged rabbit corpse on the screen and said: "It's been almost a day on the island, but you didn't find it, this rabbit doesn't have any carnivores to eat, only a few insects used to bite it, and what's even more exaggerated is that the corpse doesn't show any signs of decay on the outside." This means that microorganisms do not survive on corpses.
So island owner, do you think it's normal for such rabbits?"
Being said like this, Xu Wu suddenly discovered the blind spot, and hurriedly repeated the operation just now again to make a new sandalwood rabbit.
When it grows up, turn on the teleportation function immediately.
"Phantom Moon, teleport this rabbit over. ”
Phantom Moon nodded, and said unhurriedly: "Okay island master, the teleportation target is 4.3 kilograms, and Shenghui is 4.3 tons of biomass, are you sure?"
"Forget it, now is not the time to worry about that. Teleport. Xu Wu was a little surprised that this price was ten times the price of the island he had teleported over, but he was anxious to know his results, and he didn't care too much about these consumptions.
As soon as Xu Wu confirmed, a blue light familiar to Xu Wu lit up directly in front of the game device, and it appeared little by little, and quickly condensed and expanded, gradually forming the shape of a rabbit.
When the blue light all dissipated, Xu Wu finally saw the brown grass rabbit he was experimenting with.
Ordinary, Xu Wu's first feeling is this. This rabbit is very ordinary, with big ears drooping, and as soon as it appears, it is a little curious and keeps sniffing in the air, which is no different from ordinary rabbits.
There are no smart big ears, no special luster for the hair, and no watery special effects for the eyes, Xu Wu feels very featureless no matter how he looks at it.
It's just that none of this is the point, the point is, rabbits don't have a scent!
Xu Wu felt that he might be too far away from the rabbit, so he couldn't smell it, so he stretched out his hand and grabbed at the rabbit.
The rabbit, which was originally a little sluggish, suddenly brightened his eyes, and his powerful thighs directly exerted force, and he escaped from Xu Wu's clutches by jumping back.
It is worthy of being a hare living on the grassland, with spirituality. Xu Wu sighed in his heart.
Compared with the rabbits that are used to being raised in pet stores, hares are really much more sensitive and vigilant.
But since his last half-day trip to the island, he's not what he used to be. Whether it's strength, endurance or nerve reflexes, it's much stronger than before, and it feels close to the level of an athlete.
With a strong sense of self-confidence, he played a game of chasing with the rabbit with his hands and feet.
Five minutes later, he picked up the rabbit with a smug face, and sniffed at it like a pervert, despite the other party's screams, but still couldn't smell a trace of sandalwood.
Some only have a faint fishy smell of running on the prairie all year round.
In this way, the transformation of this rabbit really failed, and the active ingredients of the aroma should only accumulate in the cells, and failed to get out of the rabbit's body and emit it.
Even if I wanted to add these chemicals to my sweat, rabbits wouldn't be able to do it themselves.
Yes, rabbits don't have sweat glands, even if one fled frantically just now, the rabbit Xu Wu was holding didn't have any sweat on his body, so the method he just thought was naturally useless.
No animal was willing to eat modified rabbits before, probably because of the sandalwood compounds in the meat, and the taste was a little strange. There may even be a little bit of antimicrobial effect.
The epidermis is not good, the body fluids are not good, so where is the outer one?
Outside, yes, I can try adding aroma compounds to the hair, and I should be able to express the aroma by mixing it in.
Do what you think.
Xu Wu set the rabbit aside and let it roam freely in the room, then tried to poison the other rabbit again.
Hair is made up of cells, and these differentiated cells have a very specific structure. What Xu Wu has to do is to insert a hand in this differentiation process and embed the genes expressing fragrance compounds into the expression sequence of hair.
This is not a simple task, because the genes included in the gene bank do include many useless exon genes, although they indicate their functions.
Xu Wu's rash insertion of gene fragments may disrupt the expression sequence of the original gene at any time or not be expressed because it is inserted into the original exon. He can only keep trying and holding the method of casting a wide net to adjust his genes.
After failing for an unknown number of times, Xu Wu finally heard Phantom Moon say, "Island Master, this time I succeeded." ”
"Did you succeed?" Xu Wu was a little dazed, and kept giving repeated instructions, watching his biomass decline like a stream, which made him feel a little heartache at the same time, he also suddenly realized that he was not rich.
Even the fragrance of transforming a rabbit makes him break his muscles and bones, and this little biomass reserve is completely insufficient. But if it works, it's good.
The gene was immediately preserved in the gene bank, and the last item was attempted, the rabbit brain modification project. It is also the one he has the most unthinking.
Why don't you give a rabbit a whole rabbit directly according to the head of the shepherd dog? Isn't it always said that the shepherd dog has the IQ of a 7 or 8-year-old child? Xu Wu looked at the rabbit sleeping soundly at his feet, thinking that if he changed the head of a puppy inside, would the other party wag his tail and be spoiled by him?
I just don't know if it can be loaded and if it can be used.
Xu Wu complained according to his head, and even had the urge to change his brain, if only he were smarter.