"What's wrong?" everyone in the lab naturally gathered. Pen ~ fun ~ pavilion www.biquge.info

Huang Mao turned an electronic screen on the experimental table and said: "I saw a significant flow of potassium electrons before, and I confirmed that it was detected again just now, you look at the records, the average single conductance is more than 250 picosies per meter, which is ten times greater than the single channel conductance of ordinary potassium ion channels." ”

Pisi is a unit of conductivity per meter, and it is also one of the test marks of a single channel, and ten times the conductance must be abnormal, and everyone immediately gathered around to look at the record paper.

Yang Rui looked at it for a while, and then realized what had happened.

This appears to be a feature encoded by the SLO family gene.

Yang Rui quickly checked in his mind and found out the 4 members of the SLO family, and then he couldn't laugh or cry.

Huang Mao properly found the first member of the SLO family, SLO1 channel.

This is two different things from the shaker channel envisioned by Yang Rui.

The shaker channel is the first potassium channel identified in history, and it is also the first potassium channel to be identified through gene cloning. There are quite a lot of essays describing it, and it's relatively simple to do.

Yang Rui originally considered that he would first make a shaker channel, and it would be easy to add some things to form a standard paper.

You might even be able to copy a paragraph of the Shaker Channel, which will actually be published in '87, and it might be published in Nature.

But that's how unpredictable research can be sometimes.

It's not something you can do if you want to do something.

Historically, most scientific research results, especially breakthrough scientific research results, have been unexpected surprises, and in many cases, even the result of mistakes.

It is true that the shaker gene was the first potassium channel to be discovered, but the people who discovered it did not know what they would find back then.

Just like Huang Mao now, although he is mutagenic the genes of fruit flies, he doesn't know which genes he will mutagenize, or rather, after mutagenesis, he is not sure if he can find it.

After two or three months of experiments, Huang Mao did not observe the uncontrolled shaking of the calves of fruit flies like scientists did back then, on the contrary, Huang Mao found a phenomenon that is not easy to observe: an increase in electrical conductivity!

Of course, the so-called unobservability is also relative.

The shaker gene, also known as the leg shaker gene, has a problem with this gene, and the voltage of the potassium channel of the fruit fly will also have a problem, so that when the fruit fly is stunned by ether, the calf will keep shaking.

However, so many people in the Sinovel laboratory did not know hundreds or thousands of groups of mutagenesis, or did not mutagenesize to the leg-shaking gene, or mutagenesis arrived, and there was no stun of fruit flies at that time, and no such phenomenon was observed - this is also normal, if the phenomenon of gene mutation is so easy to observe, the biological world will not play with fruit flies for decades, and will still play fruit flies in the 21st century......

In terms of the unhappiness of the population, fruit flies have poured eight more molds than mice.

In contrast, the Shaker gene is easy to observe externally, while the increase in conductance observed by Huang Mao is the easiest and easiest to observe on the instrument.

Because it is a potassium ion channel, it is also necessary to observe the conductivity of potassium ions, and the SLO1 channel is even more special, and its gene performance is a 10-fold increase in conductivity.

In this way, even if the accuracy of the instrument is not high, a change of an order of magnitude is still easy to notice.

"Show me the experiment log. Yang Rui's mood was still a little excited. Potassium channels are identified by mutating genes, and the focus is on confirming the appearance of mutant genes.

In words, this is a very simple process: induce a mutation in gene A, find out that the organism has a change in B, and it is natural to determine that the function of gene A is to make B unchanged.

For example, if the shaker gene is mutated, the legs of the fruit fly are shaking, so it is determined that the function of the shaker gene is to keep the legs of the fruit fly from shaking.

Doing this is actually a very good paper, but it may not necessarily be in Nature.

If you want to determine the "Nature", you have to repeat the induction process constantly, and then determine why the legs are shaking and the legs are not shaking, and finally it turns out to be a voltage problem, plus a smooth article, which is basically a good paper in "Nature". Of course, if you want to be on "Science", you have to be literary, regardless of the content, and the writing must be a bit literary and artistic......

As a young man in the field of natural science, the hairline forced back by "Science" can circle the earth for seven and a half times when connected!

Huang Mao was also excited, and quickly found a few boxes from the top cabinet, then turned out the experimental records and handed them to Yang Rui.

His experimental records are very detailed, which is also Yang Rui's request, or experience.

20 years later, foreign laboratories have adopted video experiment recording, the purpose of which is to record the experiment in every detail.

People who have just finished the experiment will remember the experiment very clearly, but after a few weeks, months, or years, the memory will fade very well.

At the end of the day, a research institute with a good memory may remember the main or minor steps of the experiment, but which instrument was used in the experiment? Which chemical reagent was used in the experiment? 100 out of 100 people could not remember.

If it is a long-term experiment that lasts for several years, it is difficult to do the experiment again when it is re-verified or re-compared?

It's just to do it all over again, and the chemical reagents provided by different companies are also different, and the results are biased, and who is it?

In the experiment of potassium channel, Yang Rui first had to make sure that the experimental conditions did not change dramatically when the conductivity was abnormal.

Fortunately, the conductivity of the SLO channel changes very drastically, 10 times, which is basically difficult to affect by the outside world, which makes the audit work relatively easy.

When Yang Rui reviewed from beginning to end, Tu Xian and the others had already begun to congratulate Huang Mao.

Huang Mao himself suffered a little from gains and losses, and while looking at Yang Rui's unchanged expression with concern, he smiled and said, "Look at your red eyes, the second author is also jealous?"

"Who is jealous of you, our old Tu has also achieved results recently, isn't it?" Wang Xiaoyun has the advantage of being a woman, and she can speak much more casually.

Huang Mao deliberately laughed, changed it to a smug expression, and said: "The first paper on the potassium channel can take a big advantage, and the second article is not necessarily. ”

"With the progress of your current research, it's not certain that you can do the first one, isn't it?" Wang Xiaoyun poked her husband.

"I just need to do experiments, how about the paper, Yang Rui is in charge. Tu Xian ignored his wife's competitiveness.

Wang Xiaoyun punched him helplessly and said, "I'm not motivated." ”

"I'm not motivated to do experiments every day. Wei Zhenxue screamed and said, "You two in-laws are working like this, and other people's children should be in elementary school." ”

Wang Xiaoyun's face suddenly turned red and she didn't dare to speak. Both of them were busy in the lab, so naturally they didn't have time to give birth, which was a little different from the normal marriage in the 80s.

Tu Xian smiled instead and said, "Old Wei, can a single person still have children?"

Wei Zhenxue's momentum froze, he thought about it for a long time, and came up with a strange way to fight back, and said, "Do you believe that I will do IVF by myself?"

At the level of Sinovel Laboratory, there are really hardware conditions for IVF, and Wei Zhenxue also has the corresponding operating foundation.

However, this kind of second-class way of speaking is only available to Wei Zhenxue.

Normal people are at least looking for a big-breasted beauty ****** something.

Tu Xian was stunned by Wei Zhenxue's unexpected answer, and at this time, Wang Xiaoyun asked coldly: "Do you use the test tube you have filled with mung bean soup?"

"Poof......" Sun Ruyue, the experimental dog who had never dared to speak, couldn't hold back anymore and snickered.

Tu Xian and Huang Mao also coughed vigorously.

Wei Zhenxue looked as usual, and said: "I told you that the test tube is filled with mung bean porridge, and what is separated is mung bean soup." ”

……