In the laboratory, Yang Rui touched his head and wrote notes on paper, thinking about the next experimental goal. Pen Fun Pavilion wWw. ｂiquge。 ｉｎfo

On the contrary, Huang Mao and others worked diligently in front of the test bench, busy re-verifying the data to complete the minor revision of the paper.

The relationship between the experimental dog and the researcher is almost exactly the same as the relationship between a dog and a dog who throws a frisbee and walks the dog in the park.

If a cynical lab dog sees this familiar scene, he will definitely talk about lab exploitation.

However, that's how a normal lab works.

And the more high-end the laboratory, the more meaningless the simple hard work and hard work.

As the person in charge of the laboratory, Yang Rui's work in the laboratory is mainly to ask questions.

The question raised here is actually to synthesize the existing scientific research results, especially the hot spots in the scientific frontier, and put forward one's own hypotheses or assumptions.

For example, in the study of potassium channels, Yang Rui proposed a hypothesis that the structure and properties of ion channels can be verified by cloning mutant genes.

This can be said to be a very difficult and broad hypothesis, and in order to realize it, Yang Rui put forward a second hypothesis: we can target the mutagenesis of the gene we want to mutate.

Similarly, in the second layer of the hypothesis, Yang Rui has further detailed ideas, such as: directional mutagenesis of organisms, which is suitable for fruit flies.

This step-by-step assumption and hypothesis seems easy, but in fact it is difficult, and it is also the primary condition for whether you can become the person in charge of the laboratory.

The work of Huang Mao and others is actually to solve problems and complete Yang Rui's assumptions and assumptions.

In order to complete their work, Huang Mao and others also have to put forward their own hypotheses, which is also self-training for them, and the better the researcher, the faster they can become independent.

In the case of the natural sciences, assumptions and hypotheses must be based on a solid foundation, that is, the results of the pre-existing research must already be available.

Of course, how strong and how many positions there are actually depends on the level of the proposer.

If the level is low, you will obediently stand on a solid foundation and do the work that can be completed with your hand, giving human civilization a subtle force to move forward.

The higher the level, the lower the requirements for the pre-research results.

Classic examples, such as the Manhattan Project to build the atomic bomb, at the level of Oppenheimer at that time, he was not qualified to put forward this idea and hypothesis, and the leader wrote to Roosevelt that it was Einstein, and it had to be Einstein, to start such a project without anything, even with a little experimental proof.

In fact, it was scientists from Europe who were pushing for this purpose that convinced Einstein to write a letter to Roosevelt.

Otherwise, according to the normal research model, the Manhattan Project could have waited for 20 years of pre-research at a leisurely pace, at least until someone made enriched uranium before there was a basis for making a nuclear bomb.

But because it was a state of war, and because of Einstein's intervention, the pre-requirements for the Manhattan Project became lower.

Instead, the project got bigger.

What Yang Rui is considering is also the question of how big and how big his new project can be.

What he wants to do most now is PCR technology.

According to the timeline, the original author of PCR, Muris, should now start to think about the relevant ideas of PCR.

However, Muris is notoriously slacker and, to put it better, free-spirited.

Therefore, although the relevant ideas were conceived and the development of PCR was not difficult, Muric still took more than two years, and it was only partially completed around 86 years, and it was not until 87 years that it was completely completed.

It would be safer to do PCR now, but Yang Rui now only has an unpublished "Cell", and it is no problem to enter PCR immediately, but he is not sure whether he can get the corresponding reputation.

Although the scientific community is a place where results speak, after all, the scientific community is a collection of people, and there are not a few scientific researchers who have received too much praise or have not received due returns.

It is true that Muris himself is lazy enough, but the company behind him is not, on the contrary, by next year, after the company has determined the certainty of the PCR, it has invested a lot of resources in it, including a number of excellent researchers to serve as deputies to Muris.

Yang Rui thought about it, but decided to seize the advantageous position first.

If you don't have the prestige, you probably won't win the Nobel Prize, but who can be sure that you can win the Nobel Prize.

Einstein almost missed the Nobel Prize because the theory of relativity had not yet been confirmed, and finally had to use the photoelectric effect to award the prize.

A small body like Yang Rui, or rather, a small body like Muris, whether he can win the award or not really depends on his fate.

Since it is fate, there is no point in suffering.

Thinking of this, Yang Rui began to write out several key points of PCR on paper.

is also doing a PCR project, but Yang Rui is not ready to repeat the steps of Morris back then, to be precise, he can't repeat the steps of Morris back then.

Because PCR, in the final analysis, is a layer of window paper, which is easy to pierce but not easy to tear clean.

Yang Rui didn't want to poke a hole open and attract a pack of wolves.

Although it is likely to fall into scientific research competition again, from Yang Rui's point of view, he still hopes that scientific research competition will happen as late as possible.

He doesn't have a billion-dollar biotech company behind him.

Ideally, when Yang Rui has already established an unrivaled advantage, the later competitors will not have large laboratories to compete for cost and benefit considerations. As for those scientific apes who are always staring at hot spots for experiments, it is impossible to put an end to them, these scientific apes are scavengers, but it does not affect the work of the main laboratory.

"So, let's start with heat-resistant polymerase. Yang Rui's eyes were on the last step.

Although it is the last step, heat-resistant polymerase is a precursor to practical PCR technology, and it is also a necessary material, which can be made in advance to speed up the subsequent research and development, and reduce the cost.

On the other hand, this is a very critical technology, and as early as 73 years, there was a related research.

Before the introduction of PCR technology, the technology was not taken seriously, because people did not know what it could be used for, and the researchers themselves did it out of interest - in 1973, at the request of an instructor, a female student from Taiwan successfully isolated the high-temperature resistant taqDNA polymerase of the bacterium from the thermophilic bacteria found in the hot springs of Yellowstone Park, and Yang Rui did not even know if she had applied for a patent.

At the time, this may have seemed unnecessary, and no one could have imagined that it would eventually become a key part of a $300 million technology.

However, even if the other party does not have a registered patent, but has a paper in hand, it is not allowed to be preemptively registered, Yang Rui's brain turned quickly, and he immediately thought of a new way: find a new thermophilic bacteria as a research object, and use a new method to isolate high-temperature resistant polymerase.

Two researchers can apply for patents independently when they complete their own research, which is also a common way to circumvent patent barriers.

Yang Rui then rummaged through the relevant papers in his mind.

Thermophilic bacteria are microorganisms that live in high temperatures, there are many types, and there are also special researchers and laboratories to do related research.

He had to find a thermophilic bacterium that had been thoroughly studied, make sure it could be used for the isolation of high-temperature polymerases, and then start the project.

With the current scale of Sinovel Labs, it is not qualified to do the trial and error method of burning money like the top labs.

Ten minutes later, Yang Rui wrote the word "Tengchong" heavily on his notes.

The handwriting is black and thick.

The thermophilic anaerobic bacteria in Tengchong hot spring are the first extreme thermophilic bacteria found in China, and after being discovered in 98 years, they were sequenced by Chinese scientists for whole genome DNA, and thus became the first microorganism in China to decipher the genetic code.

In the Chinese biological community, Tengchong thermophilus is a famous bacteria, and Yang Rui has various studies on this microorganism in his mind, such as archaeal chromosomal proteins, new mechanisms of bacterial spore formation, and isolated extreme enzymes......

It's a natural way to isolate high-temperature polymerases.

"Let's go to the hot springs. Yang Rui suddenly raised his head, his eyes shining.

……