Yang Rui first quickly skimmed through the papers in his mind to make sure that he would not say more, say less, or even say something wrong. Pen ~ fun ~ pavilion www.biquge.info

The development of the field of biology is very fast, unlike other disciplines, the field of biology is fast, not only changing forward, horizontally, but also the concept itself.

In other words, the same word, which means one thing now, may mean something else in the blink of an eye.

To what extent, if a doctor of biology pursues romance and accidentally causes an explosion when he proposes to the goddess, he will be imprisoned for 5 years because of accidental death, and when he comes out again, the goddess's child is just in the third year of junior high school - this is not the part of the change, this is the normal situation, the part of the change is that this doctor of biology is now going to tutor the goddess's child in biology, his knowledge is outdated and backward, and he has to make up for himself first.

The world is changing so fast.

To make a comparison, Eurieut's geometry of mathematics was invented more than a thousand years ago, Newton was in junior high school with five axioms, Lu Xun was also in junior high school, and in the 21st century, Riemann corrected the last one, but because it was too difficult, not to mention that junior high school students did not learn, mathematics undergraduates did not necessarily learn.

Physics, chemistry, even metallurgy, and architecture are all commonly used knowledge from thousands of years ago, or at least hundreds of years ago.

Only biology, when the Nobel Prize is about to be awarded this year, the textbook will be changed next year.

But if you use those words ahead of your time before mainstream academia buys them, it gets a lot of fun.

This two-way selection meeting, carefully prepared by Professor Cai, is likely to turn into a huge discussion forum, completely deviating from its original purpose.

Yang Rui thought about it for almost a quarter of an hour before he said carefully: "Actually, before doing the potassium channel, I published a few papers, and when I was doing the potassium channel project, I also did a few additional small experiments, which were not written in this paper because they were not related, but it was indeed a very good subject." ”

"Oh, what is it?" Professor Cai was in charge of presiding over the meeting, and nodded slightly when he heard Yang Rui talk about the potassium channel.

Starting with the potassium channel is the most correct thing to do.

Yang Rui sat up straight and said: "It is the study of proteins in potassium channels, but it is not the current mainstream G protein, but the interaction between proteins." ”

You continue. ”

"Protein is the basic functional unit of life activities, and all physiological reactions and changes are inseparable from the interaction between proteins. When I was working on the potassium channel project, I found that we not only need to understand the structure and function of individual proteins, but also need to understand the functional processes of the internal processes of proteins......" Yang Rui paused and explained: " Repeating the study of the structure of potassium channels may be a simple and clear choice, but just doing the structure of potassium channels will soon have no way out, and the level of papers that can be published will be reduced step by step, but the interrelationship between proteins is not the same. ”

In the last sentence, Yang Rui was pure nonsense, he just looked for some papers. However, interacting proteins are an important association study of potassium channels, and Yang Rui did look at a lot when he was doing potassium channel projects.

Regarding the interacting protein, Professor Cai was noncommittal, but grasped the point of Yang Rui's words and asked: "So, the laboratory you lead will not do projects on the structure of potassium channels?"

"It's not that you can't do it at all, if you have enough money, you can do it in advance. "The biggest difference between leading the lab and joining the lab is that one is the boss, coordination and command are more important, and the other is a part-time worker, who must participate in the field.

Bosses can own multiple labs at the same time, and workers can usually only work in one lab.

For Yang Rui, if someone is willing to brush top papers in his laboratory, he has no reason to object.

As for the guidance, in fact, it didn't take much time, when he went home for the Chinese New Year, Huang Mao was not lying in the laboratory doing experiments, and his progress was not affected too much.

Good guidance is like poking a window paper, the problem is not how strong it is, but how far it is.

The purpose of raising a large group of experimental dogs is to let them realize their conjectures, sometimes in an ingenious way, and sometimes only stupid trial and error.

However, Yang Rui has done a project on the potassium channel, and it is easy to guide again, although it is not as easy as the school's chemistry teacher instructing students to do standard experiments, but it is not much more difficult than walking a dog.

And Yang Rui's answer has also been recognized by everyone, and many people have shown satisfied looks.

Yang Rui's answer shows that this student's brain is clear, not only IQ is clear, but emotional intelligence is also clear.

This is a very important point for professors and associate professors who are not familiar with Yang Rui.

The only reason why Yang Rui can sit here is that there is a "cell", if he breaks away from the potassium channel, out of his first achievement, and completely starts over, it will be rootless, and few people will build a laboratory with him.

So far, everyone present is actually thinking about building a laboratory, and they often put Yang Rui in this position.

In everyone's opinion, this is already excellent treatment.

As for the independent laboratory mentioned by Professor Cai, it is more like a picture cake, and it is much more difficult for Yang Rui to get it than it is to build a laboratory together.

Even Professor Cai himself is undecided.

In fact, in terms of achievements and papers, more than half of the scholars here are not as good as Yang Rui.

The three journals "Cell", "Nature" and "Science" are the bible in the field of scientific research, and they are hard conditions that cannot be harder, and the whole of China cannot publish 50 articles a year, and when they are assigned to the field of biology, they cannot reach 10 articles at all.

Although this number of papers basically falls under the names of top universities or institutions such as the Chinese Academy of Sciences, Peking University or Tsinghua University, most professors still do not have the opportunity or ability to publish papers on S after so many years of accumulation.

For example, Professor Tang concentrated, although he has an impact factor of more than 10. 0's syst-biol (systems biology) has published many papers, but cell is cell, and sb are always two worlds.

Of course, the impact factor of the papers published by Professor Tang Zhong is low, and there are also factors of the times, most of the Chinese scholars before the reform and opening up are biased towards publishing Chinese journals, and the Chinese journals at this time are still alive and well.

On the other hand, the current Chinese universities are not only impact factor-based.

However, no matter how you look at it, "Cell" is also a shocking power, if Yang Rui is not a student but a teacher, even if he is a junior teaching assistant who has just graduated from his senior year and stayed in school, he is also qualified to independently lead a laboratory.

Now it's 3 years away, and it still makes people feel like they can't turn around.

However, Professor Cai still gave Yang Rui a chance.

This is already his best effort to be free from the influence of tradition, inertia and worldliness.

But for now, Professor Cai didn't want to follow Yang Rui's words and asked, so he said, "You said that you have plenty of funds, how much money do you think you need?"

"$200,000, plus 3 million RMB. "Yang Rui's own potassium channel project has cost more than 600,000 US dollars, and it hurts to think about it now.

Basically, you can't get back a penny for such a project, and there is nothing to apply for a patent, and even the research conducted on this basis is still basic, and the research that needs to be studied on this basis is the technical research that is possible to apply for a patent.

However, just like movies have experimental films, literary films and commercial films, most of the things that go for awards and style are pure losses.

There are still very few projects like PCR, which are both fame and fortune.

If it weren't for the sake of accumulating prestige, Yang Rui would not have invested in making a thesis on potassium channels himself.

In the future, it is better to leave such research to national laboratories. It is better for the people to pay taxes and let the scientific research dog spoil it than to let the official ape spoil it.

Dogs are man's friends.

Professor Cai was not surprised by Yang Rui's figures, and asked rhetorically, "Is it enough?" ”

"Depending on the proficiency of the experiment, as well as luck, three months to half a year, of course, a little more money must be faster. ”

"What if it's 100,000 US dollars, 4 million yuan?" Professor Cai didn't think Yang Rui wanted much, but he was still willing to save a little foreign exchange.

Yang Rui smiled and said, "No", and said, "Most of the reagents have to be imported." ”

"If I can write a paper like this in half a year...... Professor Cai pointed to the "cell" on the table, "I will approve this funding." ”

"Thank you, Professor Cai, then I'll continue. ”

"There are not many laboratories with a budget of 200,000 US dollars and 3 million yuan at a time. Professor Cai reminded Yang Rui.

"I understand. Yang Rui knows that if the original project is redone, there will definitely be many associate professors and even professors who are willing to join, because they have a great chance of getting the honor of S first author or joint first author.

It's just that for Yang Rui, the nutritional value of such a laboratory is too low. If you want a new project next year, you have to have another meeting, maybe even harder than this time.

After a little deliberation, Yang Rui said again: "Let's talk about protein interactions first, I have learned a little bit before, there are probably so many detection methods for protein-protein interactions, I think that after the establishment of a new laboratory, we can start with the detection methods." ”

"Yes. Professor Cai said that the detection method is purely technical, relatively stable, and can also obtain a certain amount of results.

Yang Rui cleared his throat and said, "I think what I can do, first, protein affinity chromatography, second, affinity blotting, third, co-immunoprecipitation, fourth, glutathione transferase precipitation experiment, fifth, chemical cross-linking method, sixth, fluorescence resonance energy transfer method......"

When he said this, the professors who had been listening to Yang Rui with a smile slowly became serious.

It's just a list of detection methods, and many people present can do it, it's nothing more than memorization or memorization, and some of them can be remembered, not to mention.

However, Yang Rui's skillful listing shows that at least he didn't open his mouth.

Under the attention of everyone, Yang Rui said three without stage fright: "Seventh, surface plasmon resonance technology, eighth, phage display technology, ninth, yeast two-hybrid or multi-hybrid......"

When it came to the seventh, the faces of many people, including Professor Fu, became unnatural. The reason is very simple, surface plasmon resonance or something, they have heard the noun, but they don't understand the specific content.

Professor Fu immediately objected and said, "What you just said is that the new laboratory can do it, and these tests can be done?"

Testing methods are not only detection methods, but also newer and more advanced detection methods, which often represent more accurate and fresher conclusions, and when implemented, they are more and better papers.

This makes more sense than an ethereal protein.

When Yang Rui was studying, these detection methods were not new, they belonged to ordinary laboratories, and he didn't talk nonsense, and said directly: "Surface plasmon resonance technology is to obtain specific signals of biomolecular interaction through the dynamic change of SPR angle, that is, resonance angle, in the process of biological reaction. It is convenient to use a computer and software to analyze the ligand and the molecule to be tested on the chip, and it is also convenient to detect the interaction between antigens and antibodies, receptors and ligands, proteins and nucleic acids, and proteins and small molecules. ”

Professor Fu opened his mouth, unable to refute. Because he didn't understand, he could only look to both sides as if asking for help.

Lu Yueping, who had recommended Xu Anqing to Richard, couldn't see it, coughed, and said: "The principle of surface plasmon resonance technology is like this, yes, I used it when I visited the UK, but the computer technology used in this is still relatively complicated, can you do it?"

"I've used similar computers, and I have them in Sinovel Labs, so if you're interested, I can show you on the spot. Yang Rui didn't say a word, although the current computer is complex, Yang Rui doesn't think it's very difficult to learn, in fact, the academic software of later generations is not simple, the wonderful human-computer interaction and friendly interface have never belonged to the scientific research dog.

The crowd was in awe, and now, just using the application computer to do experiments is already very academic.

Professor Cai was also slightly moved, Yang Rui's youth seemed to have become an advantage at this time.

……