Shotgun is not the best genetic sequencing method.

Its accuracy is poor, so another translation of it is shotguns.

Don't count on where to play, a dozen and a large piece is its feature.

In the eyes of the most traditional biologists, this method is not very popular. Because it's counterintuitive, accuracy is also questionable.

Sanger sequencing is the sequencing of one gene at a time, exactly in the order in which the genes are arranged. The advantage of this is that after the sequencing of a gene, your map will be a little more complete.

The Human Genome Project is based on this, and Watson and others fantasize about using $3 billion, an average price of one dollar per human genome, to make a map of the human genome.

Of course, they also know that with the current difficulty of sequencing, it is not enough to sequence a gene for one dollar, but, anyway, it is just a fool, considering the dividends of technological progress, plus the cost savings of various costs, plus the additional costs in the later stage, it seems that it can almost be completed.

It just can't be done -- Dalbeko's cancer plan isn't it unfinished?

Compared with the step-by-step Sanger sequencing method, the technical characteristics of the shotgun method are actually more prominent, but it is also a little more troublesome.

Shotgun is a one-shot shot that breaks a chain of genes into pieces.

It's like a dog chain, when a shotgun goes out, it is naturally dismantled into metal rings one by one, and then the ATCG of the metal rings is measured in one go.

At this point, it's all very simple, so to speak, it's a matter of one shot.

The next step was the least favorite step of Darbeko and the others.

You need to rearrange the metal rings in the original order to create a dog chain.

This step is very difficult, so historically, shotgun was originally used for microbial sequencing. Because the genes of microorganisms are simple, they are easy to discharge.

After switching to the human genome, it is much more difficult to sort it with shotgun than a jigsaw puzzle.

Unless—

"We use computers to solve the problem of out-of-order shotguns. Yang Rui stood in the laboratory of Huarui, and without thinking about it, he said the direction of the experiment.

Sinovel Labs is his private laboratory, and all the rights are in his hands, and he doesn't need to worry about anything.

However, Huang Mao was a little worried and said: "It costs a lot of money to do the human genome project, can you earn it back?"

Yang Rui laughed hesitantly and said, "When did you care about the funds?" ”

"I'm not afraid your company will go out of business. Huang Mao said naturally: "I heard that the bankruptcy law has begun to be implemented, and insolvent companies are going to go bankrupt." ”

In the past two years, several state-owned enterprises have been forcibly closed down in China, and they have been widely reported, which is the only one that collapsed in the 80s.

"We're far from being insolvent, so you can put a hundred hearts. ”

"I want to rest assured, I'm afraid that your follow-up funds won't be able to keep up. Huang Mao said very casually.

"There will always be funds when they are needed, so let's start doing it first. Yang Rui was thinking about his hundreds of courtyard houses, as well as the more than 20 buildings in a commodity community he had just bought, and a room full of antique tea and stamps, and he always felt that he was rich.

He is, of course, rich by the standards of 30 years.

But at the end of the 80s, although the house in the capital was still out of reach for ordinary people, it was quite insufficient for scientific research. Even if it is to sell the commodity community that I just bought, that is, the daily expenses of Sinovel Labs for a few years, the bottomless pit of the Human Genome Project cannot be filled.

If you think so, you have to thank the soaring housing prices. In fact, there are a lot of dormitories for distribution in later research institutes, and the researchers' family property is mainly concentrated in self-occupied real estate, and the appreciation rate of their assets really exceeds the appreciation rate of technological development...... Theoretically, if some large research institutes sell all the land, they may be able to develop their own human genome projects......

Yang Rui's thoughts could not be known to Huang Mao, who listened to Yang Rui's words, shook his head slightly, and said: "It's okay to listen to you, but you have to pay attention, if the human genome project is pre-researched, I don't see any possibility of giving back funds." ”

The pre-research is pure basic science, and it is really rare to talk about giving back funds.

If it weren't for the later Salalah Gene Company, to be honest, Yang Rui would not be sure that the shotgun technique could be sold for money.

In fact, Salalah Genetics has not really succeeded, and its leader, Maniac, has developed the shotgun method for the Human Genome Project, despite being fooled at $300 million, and is trying to patent the human genome — if he succeeds, any future targeted drug would probably be ridiculously expensive.

The Salalah company will probably be really rich in flowing oil.

It is a pity that Comrade Venter did not succeed.

So, if you want to talk about it, the Human Genome Project really doesn't have a history of making money.

The pharmaceutical companies that made money later did not give the project team of the Human Genome Project a penny in return.

However, the fact that Americans cannot make money does not mean that Chinese cannot make money.

That's when the benefits of independence come into play.

As far as Yang Rui's current status is concerned, such as this kind of cutting-edge technology involving biology, and there is no ready-made law to use, the formulation of new laws and regulations is almost up to him.

The GMP committee or something is one nature.

Don't let a top scientist like Yang Rui make a judgment, can you find a liberal arts student to guess?

Many people still think that cloning is really copying a person.

In fact, the difference between clones and IVF is only at the genetic level, you still have to conceive a baby in October, give birth naturally or have a caesarean section, and then be educated like a normal child - if you want to get the same Hitler or Einstein, you have to see if your parents have this ability!

Qualifications to participate in the making of laws, which is the implicit power of top scientists.

From the perspective of a person's needs, why are so many people willing to immerse themselves in the painful and boring world of science? Some of them really like this world of science, and some of them use it as a springboard to enter the power hierarchy in one go.

For example, some famous economists in the 80s and 90s played a bigger role than many ministers and really changed China.

It is hard to imagine people who are miserable in stocks, happy in stocks, and depressed in stocks, and this stock market has depended on reports from several people for as long as two decades.

In the same way, people who suffer from high blood pressure, rheumatism, bone hyperplasia, and tracheitis can live a few more years and live a much easier life if a few top biologists are willing to turn to it, just like those diseases that are taken seriously, such as diabetes and AIDS, whose patients lived in very different situations in the 80s, 90s, 00s and 10s.

**The lives of patients with dysfunction have been completely changed by the little blue pill.

Before the little blue pill, many men's unspeakable secrets were really unspeakable, and they couldn't be saved, and after the little blue pills appeared, most men's difficulties were that the hairline was moving day by day, and they couldn't hide it if they wanted to.

"The issue of funding cannot be ignored. Well, if you're really worried, let's start another group and make blood pressure medication. "Yang Rui is undoubtedly ready to pull out Viagra.

The Human Genome Project is a project that costs money, and although Professor Cai promised to help find money, Yang Rui himself could not relax.

As he said, the allocation is determined by the proportion of funds, and it will take a lot of effort for him to ensure control.

If you look for something that costs less and has quick results, it is natural to think of Viagra.

Of course, today's main ingredient of Viagra is still being used as a blood pressure medication test.

Huang Mao did not doubt him, and only said: "Prescribe a group of blood pressure medication at the same time, you will be too busy." ”

Yang Rui has made a lot of new drugs, and every time he makes a lot of money, it is not surprising that he makes drugs for the sake of making money, he is only worried that the scale of Sinovel cannot support two projects.

Yang Rui shrugged his shoulders and said, "I'm more interested in the discussion of nitric oxide now, and I'm going to buy a compound to make it." ”

"Hey, shall we buy compounds?"

"Well, I'll find someone to buy it, you don't have to worry. ”

Huang Mao smiled bitterly: "How can I not worry, in the past, didn't we always make active compounds and then sell them to pharmaceutical companies for clinical trial......s?

"There are firsts for everything. Yang Rui said magnanimously: "We have money, don't worry." ”

Huang Mao was even more worried, for fear that Yang Rui would lose all his funds and buy back the compound that had no use for eggs.

(End of chapter)