"Professor Yang, we now want to do a batch of comparative experiments to improve the success rate. Su Fan and Wang Liang prepared for a few days before they went to find Yang Rui.

Although he works in a laboratory, the person in charge of the laboratory is not easy to disturb, and he is easily bullied if he does not have some dry goods in his stomach.

Yang Rui is sitting in the laboratory, and he is not pretending to be a jack of all trades.

Even if it is a sheep cloning project he started, he will not do every experiment himself, in fact, for such a large-scale project, his task is to supervise the completion of key points. As for the small parts, it's not worth the effort.

Therefore, Yang Rui did not pay the slightest attention to the problems of Su Fan and Wang Liangcai, nodded calmly, and said, "Very good." ”

Originally, doing a comparative test was the only way, but if Yang Rui taught it, this team would be too weak.

Su Fan had long been familiar with Su Fan's character, and said with a smile: "Professor Yang, we have some ideas in terms of comparative experiments, I would like you to take a look." ”

"Oh, which ones?" Yang Rui is still willing to do this kind of specific question.

"The first is the pretreatment solution. Gu Qiang used 3% sucrose, which seems to work very well, but he can't say why, considering that the sucrose solution may cause certain chemical damage to oocytes, we think it is necessary to do some investigation on the proportion of sucrose solution. Su Fan asked the first question tentatively.

Yang Rui nodded and said, "The idea is right, what's the problem?"

He has automatically and consciously put himself in the shoes of a problem-solver.

When Su Fan heard that there was a play, he hurriedly said: "I wonder if you can ask the laboratory to organize a team, or send us some additional funds and manpower to do this comparative experiment." ”

"Well......" Yang Rui was a little hesitant to continue to increase resources to Su Fan and Wang Liangcai's group.

Their progress is not slow, but such a steady and steady style is actually not very recognized by Yang Rui.

With the current economic situation in China, even Yang Rui's laboratory cannot get the international average level of funds, and it can only be said that the lower price level has reduced the operating expenses of the laboratory, but many experiments have pointed out that they are all using foreign exchange, and the funds will always be very tight.

"It's a bit of a fuss to open a research group just for a pretreatment solution. Yang Rui pondered for a moment and expressed his opposition.

Doing research at a local university seems like a good idea, and many scholars probably don't mind spending a year or two here.

But from Yang Rui's point of view, there are too many steps to cloning sheep, and the pretreatment solution is just a small point that cannot be smaller, and how can it be thoroughly studied.

Su Fan tried his best to reduce the expenses and said, "In terms of pretreatment solution, an additional 100,000 to 200,000 yuan should be about the same." ”

If you save some, 200,000 yuan can still study the pretreatment of the operating liquid relatively clearly, and 100,000 yuan is a joke.

Yang Rui shook his head even more.

200,000 yuan is also a lot, although the total amount of funds he got is eight figures, but each project and research group is a flow of money, just a pretreatment of the operating liquid costs 200,000 yuan, is not a good example.

In the final analysis, to do projects in China, it is the right way to save funds.

"I think you can adjust the direction of your development. Yang Rui thought for a moment and nodded: "Your problem is that you are worried about the chemical damage caused by the sucrose solution to the oocytes, so you want to choose a solvent with as little damage as possible, right?"

"Yes. We want to increase the success rate as much as possible, the chemical damage caused by the sucrose solution is difficult to estimate, and we are worried about the impact on the success rate in the later stage. Wang Liangcai hurriedly explained to Yang Rui.

Yang Rui said: "It is not difficult to understand that you are worried about this, but the degree of chemical damage is difficult to verify. ”

"Yes......" Wang Liangcai was a little helpless.

"I think there are two ways to think about it. Yang Rui spoke slowly.

In terms of pointing out the direction of experiments, Yang Rui has always been stingy, and he does not have too many scruples.

Seeing directions from the chaos and driving through fog is what scholars do.

If you do a good job, you will only get admiration, not doubt.

Wang Liangcai also came to his senses all of a sudden, and listened to Yang Rui with bated breath.

Yang Rui pondered his tone and said: "First, let's talk about the pretreatment operation liquid, you can not only consider it from the aspect of reducing chemical damage, but also from the direction of protecting oocytes. ”

Wang Liangcai and Su Fan looked at each other and did not say anything, this is one of the obvious strategies, but the strategy may not be realized.

Yang Rui tapped his fingers on the table twice, decided to speak more clearly, and said, "If I were to do this experiment, I would definitely not use a simple sucrose solution." I would consider giving the oocytes a more adaptable environment, for example, fetal bovine serum is a good choice. ”

"Fetal bovine serum ......" Wang Liangcai didn't expect Yang Rui to really be able to say something, and it sounded quite reasonable.

In the genetics laboratory, serum is also a commonly used reagent, and fetal bovine serum will not be unfamiliar, but if it wasn't for Yang Rui to bring it up, Wang Liangcai and Su Fan, who were full of sucrose solution, would not have thought of it.

In fact, including the Gu Qiang group, the entire laboratory has not tried to add fetal bovine serum to the operation solution.

"Let's give it a try. Wang Liangcai was a little more excited.

Judging from experience alone, adding fetal bovine serum is also a very reliable choice.

"Director, what about the second point?" Su Fan also felt reliable, he had been doing cell cloning for a long time, and fetal bovine serum had also been used, now that he thinks about it, all the indicators are very compatible.

Yang Rui smiled and said, "Second, I think your thinking still needs to change." ”

"Yes. ”

"Our goal is to clone sheep, well, clone sheep of vegetatively reproduced sheep...... All in all, this project is not a commercial project for the time being, so we only need to make a cloned sheep in the end, with an infinite success rate and not a high cost performance. ”

Yang Rui seemed to be very relaxed, as long as there was a cloned sheep or something, in the ears of Wang Liangcai and Su Fan, it was simply helpless.

But what Yang Rui said is very reasonable. If there are sufficient funds and sufficient manpower, it is naturally the easiest and easiest model to push it from the bottom up.

However, with sufficient funding and adequate staffing, it is unlikely that this will be possible.

There are very few scientific research projects that allow scholars to consume resources at will.

From Yang Rui's point of view, sheep cloning is a bottomless project.

Even in another thirty or forty years, people will not know much about the key points in cloning.

Historically, the Scots did more than 600 sets of experiments to make a cloned sheep Dolly, and if their previous research was very sufficient, even for insurance measures, two or three cloned sheep could eventually be born.

It can only be said that the appearance of the cloned sheep Dolly also has a certain element of luck.

Before it, there were several aborted cloned sheep alone, and countless hours and energy were wasted.

If Yang Rui doesn't want to spend the next 10 years on the same project, it is imperative to take a more economical approach.

Thoroughly studying every key point is neither a correct choice, nor is it possible to achieve it, and it is even more unnecessary.

As long as Yang Rui's laboratory can complete the project of cloning sheep, the previous loopholes and the key points that have not been studied clearly, will naturally have someone to check and fill in the gaps.

So many local university scholars, young people who have just debuted, and poor laboratories in the Third World have done this kind of work all their lives, and they are tired of it, and they have to give people a little chance.

Su Fan and Wang Liangcai also understood.

Yang Rui obviously disapproved of their step-by-step model, Wang Liangcai couldn't help but be a little anxious, and hurriedly said: "Professor Yang, Director Yang, cloning sheep is a series of projects, if the success rate in the early stage cannot be guaranteed, you don't even have the qualifications to enter the later stage." ”

"Didn't Gu Qiang do the nucleus?"

"Gu Qiang's method, we can't learn it. He himself knows what it is, but he doesn't know why. Wang Liangcai sighed.

Yang Rui said calmly: "Then it's good to know what it is, but not why it is." ”

"Huh?"

"We are in the same laboratory, in the same project team, divided into different research groups, just to try different paths. Since Gu Qiang has gone through one of them, then make use of it, why spend time on opening up a parallel road? My suggestion, until the part of the injection, you should only carry out the work of checking and filling in the gaps, starting from the injection, and continue to exert efforts. ”

Wang Liangcai was stunned when he heard it, but he felt empowered.

"You're right, there's no need to worry about why you use 5% concentration, 5% concentration is easy to use. Wang Liangcai couldn't help but knock on his head.

Has anyone studied an experiment very thoroughly?

Of course, there are, but those who do this step are usually studied from the back to the front, that is, the later generations study the results of the predecessors.

The scholars who are really doing groundbreaking research, who are at the forefront of science, who are like outsiders who have explored the pristine rainforest, who have the energy and time to explore the varieties and characteristics of each tree? Who has the ability to understand life under every inch of soil, or to observe the movement in the canopy of every tree?

Doing research is like driving in a foggy day, you can't stop, it's waiting to be hit by the car behind you. Don't expect to see as far as you can see, and it's good not to fall down.

Gu Qiang's experimental report is of course lacking detailed analysis, but one thing is fine, he at least proves that the road is open.

"The success rate still needs to be there, but there is no need to chase the limit, and it is better to solve the problem of whether there is or not. Yang Rui overturned Wang Liangcai and Su Fan's original ideas, but the two were not unhappy.

Valuable advice should be listened to, which is the minimum attitude of scientific research.

It's useless to make strong orders with cells, and it's even more self-defeating to get along with funds.

Wang Liangcai pondered to himself, and after a while, he said: "Since this is the case, let's repeat Gu Qiang's experiment first, and then extract the parts with a low success rate and conduct in-depth research. ”

In this case, Gu Qiang will lead for a while. Su Fan was slightly unwilling.

Wang Liangcai glanced at Su Fan and said, "First fast is not fast, cloning sheep is a big project, just like long-distance running, let's implement the following strategy." ”

Compared with Su Fan, Wang Liangcai's personality is obviously more calm, and his method is also the best way to take advantage of the advantages of the two.

Moreover, compared with the stupid method of building the foundation, the revised plan also takes into account the success rate and speed.

The most important thing is that Gu Qiang's advantage has also indirectly become the advantage of the two.

Yang Rui also nodded secretly, so that the efficiency of the laboratory could be greatly increased.

No matter how good Gu Qiang's luck is, he can't really get to the answer, and he always needs Yang Rui to correct his direction from time to time. The most important thing is that the lack of theoretical reserves makes Gu Qiang's scientific research expansion ability very weak.

For example, backtracking experiments, checking and filling gaps, and even increasing the success rate and improving the stability of experiments are not what Gu Qiang is good at.

But these capabilities are indispensable for a project as long as cloning sheep.

Wang Liangcai, on the other hand, is not a scholar who is good at creating surprises, but a steady long-distance runner. Letting go of a little insignificant face, what Wang Liang got was the opportunity to strengthen and run fast.

"Professor Yang, we still want to apply for more flight hours, especially in the evening. Wang Liangcai paused for a moment and said, "Can you give us all the time after the early morning?"

"If you apply for the night flight, you have to come every night. Yang Rui asked, "Can your body stand it?"

"I can take a two-hour nap at noon, another four hours after dinner, and the next morning for about two hours without any problems. Wang Liangcai said it easily, but the three people present knew what cruel perseverance it was to spread out the sleeping time of the whole block.

"You don't need some...... "Yang Rui has seen those who work hard, and he often asks the experimenters to work hard, but it is still rare to take the initiative to ask for long working hours.

Wang Liangcai grinned and said, "My family is not in front of me, what do I want to do in my private time." ”

Yang Rui was speechless, shrugged his shoulders, and said, "That's okay, since your family is not in the capital, you can eat a small stove in the canteen." I ask the dietitian to prepare the meal for you to ensure that it tastes good and is nutritious. By the way, I also asked for a masseur a while ago, at the national level, and when the people arrived, they would allocate an hour to you every day. ”

Su Fan's envious saliva was about to flow, but Wang Liangcai himself was at a loss.

(End of chapter)