Gu Qiang operated according to the plan, and first did synchronous estrus. Pen % fun % Pavilion www.biquge.info

Synchronous estrus is the use of certain exogenous hormones to artificially control the estrous cycle of a group of female animals, so as to plan the transfer of tissue embryos within a predetermined time.

There are many drugs that can be used for exogenous hormones, and the control effect is strong or weak, and the efficiency is high and low, but it is not very rare. What Yang Rui and Professor Wang Yong discussed that day was the existing exogenous hormones - it is easier to use ready-made nature than to redevelop them, let alone mature drugs that are not critical parts.

Of the 200 Simmental cows on site, 186 have entered the estrus stage, and it is not easy to calculate the success rate of more than 90%.

The remaining 14 looked at their companions blankly, wondering why they had suddenly become different.

A young cow is even more anxious to turn in circles.

She was surrounded by cows that were already in heat, mooing incessantly, hunched over, and impatient, and at first glance it was not quite the same as usual.

The young cow, after twenty or twenty-five turns, made a resolute decision, and saw that she also hunched her waist, and learned from the other cows to give up the feed in the trough, and then she also raised her hooves in a hurry, and turned her head to the barn where the bull was.

Eventually, the heifer managed to get the attention of a veterinarian.

He put on his heavy rain boots again, took the utensils, and went up to inspect them.

Gu Qiang also looked up and asked, "Is it in heat?"

Comrade Qin Yue, a veterinarian from Zhongmu, looked at the cattle for a while, came back and took off his glasses, wiped them, and said, "No, it's still 186." ”

"Doesn't it? ”

"It's loaded. Qin Yue put on his glasses and replied concisely.

The crowd around the bullpen let out a soft laugh.

Gu Qiang shook his head in astonishment.

He spent more time in the lab than he spent in the field. However, this is the case with most researchers, and the people who are sent to the site are often the least senior members of the research group or project team, either assistant researchers, or doctoral students, or graduate students, and sometimes even undergraduate students.

However, genetic engineering labs do not have low-seniority laboratory dogs to exploit.

In the genetic engineering laboratory, Yang Rui has the lowest seniority, and he has only three years of scientific research experience, that is, he is about the same as an ordinary master's graduate, and in the research institute, it is equivalent to the qualifications of an assistant researcher - so Gu Qiang can only go out in person.

Fortunately, for the sake of 200 Simmental cattle, the China Animal Husbandry Corporation has fully allocated auxiliary manpower.

I only heard Gu Qiang shouting "Super ovulation begins", and there are cattle herders who bring the cows in estrus one by one, first weighing, and then making records.

Four veterinarians lined up in a row to inject the cows in turn.

Qin Yue is at the forefront, responsible for not only administering injections, but also reporting numbers and judging the health status of the cattle.

Gu Qiang stood behind the veterinarians, sucked the gonadotropins in the syringes according to the reported weight data, and then handed them to the veterinarians respectively.

It was Yu Xing'an who helped Gu Qiang, who stared at the veterinarians to make sure that all the drugs in the needles were injected into the correct cows.

After the entire injection process was completed, Yu Xingan poured a large amount of foaming water on the veterinarians in the name of disinfection.

Even if someone secretly squeezes a little bit of the potion on their body, the whole barrel of disinfectant will destroy the activity of the potion.

Director Tie from Qingmu, who has been reporting in the capital for a period of time, also came here today, seeing this scene, he couldn't help but smile slightly, and said to the arrogant Director Mei next to him: "Look at it, no matter how much money you in the herdsman give, you are still not trusted." ”

"There's no need to sow discord, it's too tender for you to play this. "The arrogant Director Mei has always despised the broad-shouldered and strong Director Tie's ability to scheming.

Director Tie smiled and said, "Don't sow discord, you see, it's just a gonadotropin, and they are all hiding for fear that you will know." ”

Director Mei's face was also a little unsightly, and said: "They may have researched some special hormones, and it is normal not to let other units know." ”

"What if you can get five eggs at a time? I heard that the latest foreign technology, ovulation six or seven gonadotropins, this thing is not used much, it's a big deal to spend money to buy it, you give less money?" Director Tie didn't care about the relationship between the Haidian District Genetic Engineering Laboratory and the China Animal Husbandry Corporation, he just wanted to respond to Comrade Lao Mei now. Of course, it would be better if the relationship between the two sides went bad, and he dreamed of monopolizing this resource.

Director Mei has been at odds with Director Tie since he was in school, but he secretly agrees with what he just said.

Director Mei twisted his neck uncomfortably, and said: "Yang Rui is a young man after all, this Haidian District Genetic Engineering Laboratory is also a new laboratory, and they have finally developed a new technology, even if it is only 60% of the efficiency of foreign products, it is worthy of respect." ”

"Hey, working in Beijing is different, the official saying is a string of strings. Director Tie laughed twice, but shouted to the veterinarian in front of him, "Do you see any eggs?"

He asked, naturally, how many eggs were released during ovulation.

Anyone who has studied biology in junior high school knows that an embryo comes from the union of a sperm and an egg. The number of sperm released by a bull at one time is extremely large, which can be described as thousands, and eggs are extremely scarce.

For a cow with only one foetus, the number of eggs is even smaller, often only three or four, in other words, the number of embryos available for embryo transfer is actually determined by the number of eggs.

If a cow ovifies 4 eggs at a time, and all of them develop into A-class embryos, it is possible to obtain 8 half-embryos after one embryo segmentation.

Even if the success rate of the half-embryo transfer is 50%, it is double and double the reproductive efficiency for a well-bred cattle, not to mention that multiple ovulations can be performed within a year.

However, commercial operations have never been able to achieve ideal data, and the desire of capital cannot be satisfied by 4 or 8 times the efficiency.

In developed countries outside of China, the reason why biotech companies have worked tirelessly to research embryo transfer technology is that they have improved the success rate in all aspects.

For example, in the process of collecting embryos, the purpose of gonadotropin injection is to obtain more than 4 eggs......

In an era when an egg basically represents a cow, it is inevitable to develop better and better gonadotropins.

From the seventies to the eighties, gonadotropins were polarized, both to get more eggs and to reduce side effects and increase the proportion of available embryos.

In this subdivision, it is natural that domestic research institutes lagged behind again, and it should be said that there was basically no research, and the technology introduced from Eastern Europe in the fifties and sixties was introduced.

However, the difference between the five eggs and the seven eggs is not one man, but two cows.

Veterinarian Qin Yue, stepping on rain boots and holding a record book, walked over, shouted "Director Mei", and then shouted "Director Tie", and then asked softly: "You called me?"

"I called you, you Director Mei want to know, how many eggs does a cow have?"

"It's not very clear yet. Qin Yue looked at the notebook and hesitated.

"Just say you know. "Director Tie has been a ranch all his life, and he leaves no room for words.

Qin Yue turned his face to Director Mei.

Director Mei sighed and said, "If you ask, you will say, you know how much to say." ”

"Yes. Qin Yue looked down at the book again, and said, "We have checked twelve cows so far, and the corresponding number of ovulations is 10, 12, 11, 10, 13, 9, 12......"

"Wait, what I'm asking you about is the number of eggs, how many cows are there?" said Director Tie's eyes widened.

Qin Yue held up the frame and said, "I'm talking about a cow." (To be continued.) )