Chapter 988: Details
Yang Rui looked at Gu Qiang's wild laughter inexplicably.
A normal person, under such a gaze, should not be able to laugh awkwardly for ten seconds at most.
However, Gu Qiang is a human being? he is a man with the spirit of a teddy who pierces the heavens and the earth.
Teddy can catch a shabby rag doll under the gaze of everyone and even dogs; Teddy can catch a shopping thigh under the gaze of everyone and even dogs; Teddy can slowly move in the air frequently and efficiently under the gaze of everyone and even dogs; Comrade Gu Qiang can naturally laugh heartily and chic under the gaze of everyone and even all (scientific research) dogs.
Yang Rui couldn't help but sigh deeply.
Everybody says Teddy is good, that's when you don't have white slime stuck to your legs, everybody says technicians have personalities, that's when your ears don't hear them laughing.
We are doing repeated experiments of frozen embryos, which is not innovative, and there is no need to be excited. ”
"Haha......" Gu Qiang couldn't help but laugh again when he heard what Yang Rui said, and said If I have a tape recorder at hand, I will record it and put it on the people of the Chinese Academy of Sciences to listen to, so that they can listen to it!"
Gu Qiang coughed, cleared his throat, learned Yang Rui's voice, and said that we are doing a repeat experiment of frozen embryos, which is not innovative. ”
Gu Qiang laughed wildly again, the people of the Chinese Academy of Sciences are going crazy, hahahaha......"
I have to say that Gu Qiang parrots his tongue, which is really cheap enough.
Yang Rui sighed again, working with such a person is indeed troublesome enough.
This time, Yang Rui felt that it was not good to move out of the name of the person in charge of the laboratory again, he stood up a little straighter, Researcher Daogu, you may not, there has always been laughter outside the window of our ion channel laboratory. ”
"Huh?" Gu Qiang didn't understand.
"When the ion channel laboratory was not built, I took an experimental group to do a potassium ion channel project, and the laughter was even louder at that time. After all, the study of potassium ion channels at that time was a worldwide innovation. Yang Rui glanced at Gu Qiang, and said slowly, no one thought that I, who was still a student at the time, could lead the team to make a world-class basic biology project. Within Peking University, Professor Richard of Berkeley University is also supported......"
The researchers in the laboratory may not have experienced that scene, but when they think back then, as a member of the ion channel laboratory, they still unconsciously puff up their chests.
Yang Rui looked at Gu Qiang with a smile, moved his lips, and said Later, when I did PCR, no one was optimistic, and the smile spread abroad, and there was a lawsuit for this, but as a result, I still kicked those smiling faces. ”
"And then there's the drug development of deferiprone that you may not know about. Yang Rui looked at Gu Qiang with a disdainful expression unabashedly, and said, But I was embarrassed to laugh back then, but now I am embarrassed to show my face in front of me. ”
Yang Rui paused slightly, faced Gu Qiang, and asked Now, I said that I want to do a frozen embryo experiment, who would dare to laugh?"
The researchers in the laboratory couldn't help but look at Gu Qiang.
The smile on Gu Qiang's face was long gone.
He seems to have only now recalled that Yang Rui was a scholar with articles in the three major CNS journals.
In China, there are naturally some scholars who can do this.
However, whoever can do this is not the best academic.
Such a scholar, who would dare to laugh at the repetition of foreign experiments?
Gu Qiang suddenly felt that it seemed that he had laughed a little early.
"Genetic engineering, and basic biology, are still different. Gu Qiang tried his best to find a similar explanation.
Mainly face-to-face, Gu Qiang is really difficult, Yang Rui, who is so young in front of him, can make so many achievements.
Moreover, just like he said, Yang Rui has never done genetic engineering after all, and repeats the experiment just by saying that the experiment is repeated.
Repeated experiments are not the same, some old experiments, such as those done in the middle school laboratory, all the steps and details are very clear, even where the error will be, there is a professional analysis, just pull a student out, as long as you know the words, the meaning on the paper, a little flower, there is no failure to do those experiments.
However, back then, some of the experiments in the middle school laboratory were not kept secret.
It is true that there will be papers in journals, and there will be experimental procedures in the papers, but if you want to repeat these experiments without details, you will always have to pay some inquiry.
The more complex the experiment, the more so.
Like the transfer of frozen embryos, there are many details, and if you are not careful, you can make mistakes.
Living embryos, frozen and resuitized, but also transplanted into the organism, and then pregnant with cubs, a little mistake, may lose the whole picture, and because the process is too long, it takes too long, the cost of trial and error is quite high.
If not, how can domestic laboratories give up this opportunity to "fill the domestic gap".
Yang Rui's understanding is consistent with Gu Qiang's, genetic engineering and basic biology are indeed different, but for him, the difficulty of repeating experiments is completely different.
In 85 years, foreign institutions were of course on guard against it and refused to divulge the details of frozen embryo transfers.
Because although they have completed the frozen embryo transfer of mice, cattle, and sheep, they have not made frozen embryo transfer for pigs, dogs, monkeys, etc. - the frozen embryo transfer of the first few important animals can be easily ceded to competitors in a proper CNS-level paper.
However, after the mid-90s, the information was worthless.
Yang Rui is checking public information, and he can find countless information.
In particular, some master's and doctoral dissertations, due to their long length, are mostly repetitive experiments, and the content is really detailed - not detailed, doctoral dissertations and master's theses are required to specify the number of pages, often 100 pages and 200 pages of papers, you don't write detailed experimental details, do you still want to discuss industry gossip?
Even if some papers have problems such as false data, it is still possible to refer to each other and find specific details.
The corners of Yang Rui's mouth curled up, but he stopped playing with Gu Qiang, and just started experimenting. ”
The problem that needs to be solved is solved, and he doesn't need Gu Qiang to be convinced.
Gu Qiang obediently stood behind the experimental table and began new preparations.
"Have you ever done an experiment to freeze embryos?". Yang Rui took the sorted outline and also reviewed the various steps in his mind.
Gu Qiangdao has read the information, but has not operated it on the spot, and the equipment is too expensive. ”
He looked left and right, and pouted a little unconvinced.
The ion channel laboratory is engaged in basic biology, and it can make up complete genetic engineering equipment, which is the envy of ordinary scientific research institutions.
"I haven't done it well, I have a few experimental designs here, let's do a cross-comparison. Yang Rui said and handed a piece of paper to Gu Qiang.
"Are you willing to take the experimental design?" Gu Qiang snorted twice, picked it up and looked at it, and while watching, the embryo was put into the balance solution to balance, tube-loaded, cooled, ice-planted, and then balanced...... Hehe, I've read this kind of article a long time ago, how many degrees does the cooling drop?
His question, apart from the tone, could not be more realistic.
Yang Rui was not surprised to talk about the balance liquid first. We use glycerin. ”
"Glycerol?Glycerol regulates cell dehydration and protects protein structure, but glycerol is only suitable for slow freezing. ”
"We also don't have the equipment for flash freezing. ”
"What about the concentration?"
"Tentatively select two percent of each liter, use buffered PBS solution to configure, PH should be guaranteed to be between 7.2 and 7.4......" Yang Rui said these, all of which are details can not be more detailed, such as PH value, its fluctuation range, in ordinary experiments, I don't care, just look at the PH of the final result. But here, it is not possible, which requires that in the process of configuration, we must always pay attention to PH, and adjust the order and rate of filling, if there is no relevant information, trial and error can be ten years.
Gu Qiang glanced at Yang Rui a little weirdly, but he muttered that people are more popular than dead people. ”
He thought that this information was obtained by Yang Rui from a foreign scholar, the so-called academic connections, sometimes it is a very unfair unspoken rule, Gu Qiang does not know a foreign scholar, no matter how good his experimental skills are, he has no chance to show, although Yang Rui does not know the experimental operation of genetics, but he can get the relevant information, but he is firmly in the position of the project leader.
The modulation of the balance liquid itself was fine, Gu Qiang didn't say more, and concentrated on the operation.
After a while, Xu Zhengping left, Director Dao Yang, the list of the third batch of visitors was confirmed, you see the arrangement, mainly for laboratory visits. ”
"Just let them come and see the frozen embryo transfer program. Yang Rui replied cleanly.
Xu Zhengping was stunned, high-tech projects are naturally good, but they must have results to look good.
Gu Qiang didn't look back, I can't guarantee that I can do it. ”
"You just have to make no mistakes. Yang Rui replied even more annoyingly.
Xu Zhengping was a little uneasy, but he still nodded, approached Yang Rui again, and whispered This time, Professor Zhang Feng from Nannong and Professor Ouyang Shi from Shandong University came. ”
"Two big cows?"
"Yes. ”
"I'll tell Professor Cai okay. Yang Rui felt that it was still difficult to eat two big cows in one go, and immediately thought of Professor Cai, who had a good appetite. He'd made a promise about it before.
Gu Qiang frowned when he heard the word Shanda, but the movements in his hands were more careful.
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(To be continued.) )