Yang Rui drove the car from the vicinity of Shichahai to the laboratory, that is, twenty or thirty minutes, the capital without traffic jams, the young people in the future are probably ...... Well, you can feel it during the Spring Festival.

The researchers in the laboratory do not have Yang Rui's relaxed mentality.

Wang Liangcai, Su Fan, Gu Qiang, Tian Bing, and many of the auxiliary researchers who were transferred were all a little nervous, either with solemn expressions, solemn expressions, or very heavy weights......

Judging from the results of the experiment, they felt that they had made a breakthrough, and that they had already carried out repeated experiments - pulling the lab boss back from bed so late had to be sure and determined.

The reason why there are all kinds of people is to gather people to carry out repeated experiments and verification.

Of course, the main reason is that everyone didn't get off work in the first place.

The food and drink in the laboratory are good, no matter how late you stay, the chef will help you make supper, and you can pack the leftovers home, and there is overtime pay, who is okay to go home.

Besides, what can experimental dogs do after work?

Just kidding, can an experimental dog have a wife?

Does the wife of the experimental dog like to chat? If she likes to chat, why should she marry a taciturn experimental dog?

The above all illustrates one point.

A group of extremely bored guys, who have spent hours in the lab, are still not sure that they have really achieved a breakthrough.

What they're doing is "cloning," and it's cloning mammals.

Such a project has the potential to produce dozens of top-notch papers at any time. It's no exaggeration to say that if the sheep cloning project were to publish papers in a centralized manner, half of the space would have to be freed up for preparation, from "Cell" to "Nature" to "Science".

In other words, if the immediate results are confirmed, the research group has at least booked a CNS.

"Wang Liangcai, was it done by your group? Yang Rui glanced at the door, hung up his coat, and talked while walking.

Wang Liangcai coughed a little nervously and said: "Yes, we have completed the steps of nucleus, and at present, the cells after nucleus injection have met the requirements of all indicators." Some indicators also exceeded expectations. ”

"Our group has also completed a check. Gu Qiang was stuck in time and also interjected: "Moreover, our group completed it a little earlier than Wang Liangcai's group." ”

Yang Rui turned his head to look at Gu Qiang and asked, "Why didn't you call me?"

"How can I carry it all on my back. Gu Qiang said in a slightly lower voice and said, "We want to do a good job of repeating the experiment and then call you." ”

"Are you done?" asked Yang Rui.

"Also...... Not yet. Gu Qiang replied a little helplessly. This is not the first time that his group has completed the verification step, in fact, his group was the first to complete the verification step.

However, difficulties are easy to overcome, and experience is rare.

Continuous completion of nucleation does not mean that they will be able to get the injected egg cells that meet the requirements, and even if they get the injected egg cells that meet the requirements, it does not mean that they will be able to repeat them smoothly.

It is difficult to repeat the experiment, which is a big problem encountered by Gu Qiang's group.

He always relied on luck to overcome difficulties again and again.

However, repeating experiments must not only be done by yourself, but also by others.

Only in this way can you get a helping hand and ensure a stable supply of nucleated oocytes.

In order to complete the cloning of sheep, a large number of nucleated egg cells are required.

Yang Rui's plan is to prepare 1,000 injections of eggs, because historically, the Scots used about 550 qualified oocytes to get a cloned sheep Dolly, the only cloned sheep, and the pregnancy process was quite dangerous, and several sheep companions were lost in a circle.

Now, Yang Rui has carried out the sheep cloning project several years in advance, and the domestic conditions in all aspects are weaker than those in the UK - taking care of the ewes itself is a technical job, and the abused oocytes are even more congenital deficiencies......

Therefore, when Gu Qiang's experimental group could not stably repeat the experiment, it was not enough to obtain only one nucleated cell that did not break down, even if it was obtained to meet the target.

Yang Rui didn't need to ask carefully, he could guess that there was a problem with Gu Qiang's group.

However, he rushed over today, not to be Gu Qiang's confidant brother.

Yang Rui just nodded slightly to Gu Qiang, then turned to Wang Liangcai and said, "Give me the experiment record, let's start talking." ”

Yang Rui stretched out his hand to get the record, turned his finger while looking at it, and motioned for Wang Liangcai to proceed synchronously.

Wang Liangcai took a deep breath and said, "We have improved the method of denuclearization. ”

"Oh, second improvement, good. Yang Rui's attention was instantly attracted.

The method he gave was already very advanced, which made him want to hear Wang Liangcai's improvement method.

"I said the main part. Wang Liangcai said: "We used a spindle to jam the enucleation needle, and then sucked up the fragmented nucleus. ”

"Yes. ”

"The improved method still uses the tougher characteristics of the spindle, but we suck part of the spindle directly in. ”

"Oh?"

"The advantage of this is that it is possible to aspirate the nucleus intact, with less contamination and at a faster rate. Wang Liangcai paused and said, "Although the success rate has also decreased a little, I think it is worth it." ”

Of course it's worth it. Yang Rui greatly appreciated Wang Liangcai's experimental talent, and even had some ...... A little jealousy.

That's what the so-called experimental genius is.

In order not to be too advanced, Yang Rui actually did not give the most perfect solution, but only gave a basic version of the plan, simply using the spindle to make a fuss.

Who would have thought that Wang Liang had just learned the basic version of the things, so he would give it an advanced level by himself.

This kind of acumen and execution is truly breathtaking.

Yang Rui originally planned to explain the improvement of the test method when it was time for large-scale nucleation of cells-only in the experimental stage, which could not delay much work, and had the benefit of step-by-step practice.

Who would have thought that Wang Liangcai would solve the mystery like this.

Yang Rui nodded helplessly and in agreement, and said, "The idea is very creative, what is the result?"

"Comparative experiments have shown significance. "Significance refers to the data, Wang Liangcai omitted it at this time, to the layman, it seems to be a bit of a black word, but in the laboratory there is no slightest disobedience.

The results of the experiment are naturally significant. Yang Rui smiled to himself, flipped to the part of the experimental record, and checked it.

Although it was an expected result, he still needed to verify it carefully.

Academic fraud happens in the details, and no one is going to change the final numbers and make the results match. Of course, there are always stupid people, but they are not significant.

The normal mode of academic fraud is to start from the original data, change the initial numbers, and then make the following numbers through the normal operation icons, which seems to be troublesome.

The most important part of the responsibility of the project leader, or the director of the laboratory, is to suppress the impulse of other scholars in the laboratory to cheat, and at the same time, bear all the consequences of academic fraud.

Therefore, the beautiful Japanese researcher was still quibbling after the fraud, and her mentor committed suicide.

At this point, the project leader is a bit like the head chef of the western kitchen, he doesn't have to do it himself, but he has to check everyone's work and do the final quality control work.

Wang Liangcai was confident in the experimental records, and was not worried about it, and waited for Yang Rui to look at it for a while before continuing: "The pitting part is only a small modification, not the most important breakthrough. ”

"I'm a little more confident in your group. Yang Rui's face couldn't help but smile, which made the other researchers breathe a sigh of relief.

Wang Liangcai also quietly breathed a sigh of relief.

Wang Liangcai likes Yang Rui's way of speaking and doing things.

He likes Yang Rui to use "your group" or "your group" to describe it. The common habit of domestic scholars is "your group", and the difference between the two is individualism or collectivism.

In the 80s, individualism was undoubtedly condemned and oppressed by collectivism. If you change a person or a laboratory, the three words "your group" are enough to have a criticism meeting.

However, Yang Rui can say whatever he wants, and those who accept it don't have to worry about bad influence.

The three words "your group" carry Yang Rui's authoritative Yu Ze, so that Wang Liangcai, as a secondee, can also eat in the genetic engineering laboratory.

As for Yang Rui's way of doing things, he has won the praise of researchers.

Everyone is too busy to pass on the ancestry, you are still blind ** meeting or something, believe it or not, I spray you in the face?

Wang Liangcai smiled, and then said: "In terms of nucleus, we have decided to learn from your previous experience in technology, use pulses to penetrate the plasma membrane, and at the same time, use a transparent bag for fixation. ”

"That's where you have to use a strong pulse. Yang Rui is very familiar with the details of the experiment, and the relevant research on cloning sheep is really full of sweat.

Wang Liangcai saw that Yang Rui understood, and said "That's right" excitedly, and said: "We have done a lot of comparative experiments, and finally determined the minimum intensity pulse ...... that can break the plasma membrane."

Yang Rui listened again.

Wang Liangcai is talking about a very small point, but a correct fulcrum is to be able to warp ...... At least blow up a piece of the earth.

The use of pulses to impact the ulcer membrane is a great change compared with the previous nucleation method, and in this way, there will also be some problems, but if it is solved, it is indeed a broad road.

"Professor Yang, what do you think?" Wang Liangcai's soft inquiry woke Yang Rui up from his contemplation.

"I still need to see other people's repeated experiments, but, Lao Xu, I can focus on the activation of reconstituted embryos and in vitro culture for public relations. What Yang Rui said is equivalent to advancing the progress of the project.

Everyone in the laboratory cheered quietly.

Only Gu Qiang, who paced back to the backyard with some interest, stared at the incense burner in front of the courtyard wall, silently in a daze.

I feel that the experiment is still going very smoothly, but why can't Wang Liangcai do it?

No, I used to do more smoothly than Wang Liangcai, but which variable has changed?

Gu Qiang looked ahead blankly, eager to make a calculation to solve this problem.

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