"The crystallization step is the most important, you must pay attention to it again, keep the process in mind, and silently recite the steps in your heart......"

"Dissolving this step is the most important, absolutely pay attention to pay attention to see if there is any precipitation? do not waste raw materials, in addition, always remember that after a dissolution, no more solvent is allowed, so be sure to calculate the amount used. Pen Fun Pavilion www.biquge.info"

"Don't let me count here, experiment experiment, you have to do it to count!"

Tao Xuelin gave orders in circles in the ion channel laboratory.

This is what he is very good at, before becoming a full professor, Tao Xuelin's main job was to lead the people under the professor to work. Of course, before the 80s, the most work was actually the cooperation of various units, and the superior leaders had any ideas, whether it was the leaders of Peking University, the leaders of the Ministry of Education, the leaders of the Ministry of Culture, the leaders of the Academy of Sciences, or even the leaders of Beijing.

In the early ten years, the projects of the research team were diverse, especially for the teachers of the biology department, even more so, simpler, how to improve the effectiveness of manure, more complicated, how to design a new manure, more difficult, what to eat to produce better manure......

At that time, it was not a school or a research institute to work independently, although many projects could be done by only one or two people, but in the eyes of the leaders, how could the projects done by one or two people appear to be taken seriously, and how could the projects that were finally come up with be replaced.

The signboard of Peking University cannot be ignored, so for a long time, Tao Xuelin has been doing "big projects".

For a while, Tao Xuelin was depressed, in fact, for a while, many academic elites were depressed, always feeling that they had wasted their time and talent......

However, experience is never wasted.

Although Tao Xuelin has done many useless big projects, the experience of big projects has been preserved after all.

In the ion channel laboratory, he also quickly found his place in managing the production of bovine rhodopsin protein.

How to produce is Su Xiankai's job, and in the early days, he was trying various ways to produce at the same time.

With the improvement of the progress of the experiment, the nature of Su Xiankai's work has actually slowly changed.

Just like Yang Rui's production method of coenzyme Q10 back then, the research that Su Xiankai wanted to do was actually slowly biased towards the research of production methods, and when his research became more and more extensive, bovine rhodopsin protein actually became the same as coenzyme Q10, and it could be divided and cooperated and entered a specialized production state.

In the 80s, biopharmaceuticals were actually produced in the laboratory, including the WestJet factory established by Yang Rui, a total of one or twenty people, twenty or thirty people, can form the production department of the factory, and the instruments operated by everyone are similar to the enlarged version of the laboratory, and some do not even need to be enlarged, and the laboratory is used for production.

The extraction of coenzyme Q10 from bovine hearts, the extraction of insulin from bovine pancreas, and the extraction of bovine rhodopsin protein from bovine eyes are all one type of model.

Yang Rui and Su Xiankai helped a little, and passed on all the skills they currently mastered to Tao Xuelin.

Tao Xuelin only took a few days to increase the production capacity of bovine rhodopsin protein several times.

This is not a multiple-increased increase in the initial stage, but an improvement after Su Xiankai has been deeply cultivated for a long time.

The addition of Tao Xuelin also made the yield of bovine rhodopsin protein not only exceed Yang Rui's requirements by a hundred times, but also exceed.

This is the embodiment of Tao Xuelin's management ability.

The same allocation of 6 scientific research dogs, the improvement of the Gu Qiang group was not so obvious.

Although Gu Qiang's experimental ability is strong, management is not a strong point, on the contrary, because Gu Qiang's personality is too strong, these graduate students brought by Tao Xuelin do not like to cooperate with Gu Qiang.

However, no one dared not listen to Tao Xuelin's order. Unless there is anyone who doesn't want to graduate, otherwise, for graduate students, the mentor is the sky, the mentor is the earth, the mentor is the brilliant and great flower, and the mentor is the person who you tortured to death in your dreams thousands of times, but let you pretend to enjoy it during the day.

Tao Xuelin's mental state is also very good, putting his laboratory aside and entering Yang Rui's laboratory is also a huge challenge for Tao Xuelin, who has seen forty years. It's nice to be able to find your place quickly.

He paced around the large laboratory assigned to him, watching the work of the students one by one, more seriously than when he was in his own laboratory.

Gu Qiang was a little anxious, and looked at the energetic Tao Xuelin angrily and helplessly, and said: "It used to be a Su Xiankai, and now there is another Tao Xuelin, and now there are more people in the ox violetin proteome than us, how can so many materials be used up." ”

Tian Bing glanced at him and said, "I haven't heard of a lot of materials." ”

"Just ask if you're afraid. Gu Qiang asked while working.

Tian Bing snorted twice and said, "There's nothing to be afraid of, Director Yang doesn't have any quantity requirements for me." ”

Inserting soluble protein fragments into G protein-coupled receptors is easier said than done.

G protein-coupled receptors are still proteins, and the size of proteins is much smaller than that of microorganisms, in other words, you can't see it with an ordinary microscope.

It's not easy to insert something into something you can see, it's too hard to insert something into something you can't see.

Therefore, it is necessary to verify the stability of the receptor, which is even more difficult.

Tian Bing himself was in pain, but this did not prevent him from mocking Gu Qiang.

Gu Qiang pursed his lips and said, "Yang Rui doesn't have quantitative requirements for you, it depends on how much you haven't made any results." ”

Tian Bing smiled: "We just started to do it here, what should you do? If you can't do it again, let alone Yang Rui, Su Xiankai will have to kill you." ”

If the oxazopsin protein made by staying up late was wasted, Su Xiankai would indeed beat people angrily.

"It's really not good, so I stirred up the bovine rhodopsin protein he sent. Gu Qiang was ruthless, he has been really tired recently, especially after Tao Xuelin joined, let alone sleeping, he trotted to eat.

Tian Bing laughed out loud and said, "Who wouldn't waste it, be careful that Director Yang hangs you up and beats you." ”

Hanging up and hitting is a word that Yang Rui often says recently, and everyone else has learned it.

Gu Qiang argued: "Who said that a pot of stirring is a waste, anyway, in the end it is necessary to enter the centrifuge, the early processing is carried out unified, that is, the yield is lower, and the efficiency is also improved, right......?

"If I hadn't known you were, I would have believed you. ”

"It can't be said that it's all nonsense," Gu Qiang said to himself, falling into a state ......of contemplation.

"What do you want?" Tian Bing had a bad premonition in his heart.

Gu Qiang didn't say anything, and after a while, he said: "Actually, if you think about it carefully, I wasn't talking nonsense just now, what I said made some sense." ”

"What's the point? You're wasting at least 10% of your protein like this. ”

"Not necessarily. Pretreatment is inherently lossy, and the pretreatment is done in batches, and the loss is a little larger, but we can find a way to optimize it, and compared with before, it is more than 5% at most......"

Gu Qiang imagined the future with a beautiful smile on his face.

Tian Bing reminded: "5% is not a lot. Su Xiankai's output has increased to more than 100 times the original, and if you think about it, if you increase the loss by 5%, it is equivalent to wasting five or six times the previous output. ”

"When the bovine rhodopsin protein is not enough, 5% must not be wasted, and now the conditions have changed? What is Yang Rui's purpose in increasing our production? Su Xiankai and Tao Xuelin are busy making bovine rhodopsin protein for what? It's not ...... yet."

"It's not for you to waste. ”

"It's so that we have a choice. Gu Qiang was full of words, and the more he spoke, the more he felt that it made sense, and after a while, he nodded fiercely and said, "I think we can try." ”

The corners of Tian Bing's eyes jumped desperately and asked, "What do you want to try?"

"As far as you propose, stir it in a pot. ”

"You brought it up, not me. Tian Bing hurriedly affirmed, and tried to dispel Gu Qiang's concerns, and said: "Don't mess around, now there are more people, you are like Tao Xuelin, you have adjusted the personnel arrangement reasonably, even if you work overtime, you can't work for a few hours." If a pot of agitation does not work, not only the loss of pretreatment, but also the pollution in your later stage is also very large, and what should you do when it comes to separation and treatment. ”

"Do what you have to do. Gu Qiang paused as he spoke, and said, "We used to do it separately, just like cooking in the kitchen, you have to prepare the dishes one by one, and each dish is a different way of preparation." Dealing with it together, it's like hot pot, didn't you eat a lot of Donglai Shun's copper pot last time?

Tian Bing looked at Gu Qiang like a madman, and said, "You were driven crazy by the task." ”

"yes, I'm driven crazy. Gu Qiang had an expression of lying down casually, clapped his hands, and said, "Anyway, I have to try it once." ”

"If Yang Rui knew that you had wasted so much bovine rhodopsin protein, he would really hang you up and beat you. ”

"Hot pot, does it have to be bad for stir-frying?" Gu Qiang asked again.

Tian Bing said: "You don't want to explain it to Yang Rui like this." ”

"Talking about hot pot and stir-frying, Yang Rui should be more understanding. Gu Qiang looked ahead and consciously found a perfect reason.

……