Su Xiankai became more and more proficient, and soon became an expert in bovine rhodopsin protein. Pen & Fun & Pavilion www.biquge.info

Life in the lab is actually like this, when the lab is just starting to decide what to do, most of the participants are actually blinded and can only learn by doing in the later research.

This is probably not quite what ordinary people think, or rather, it is very different from the life of industry and politics.

Repetition is probably one of the most accustomed ways of working in industry and politics. For example, in a private company, the staff he wants most are experienced employees. What kind of experience has been experienced, or is familiar with such and such a job, so as to facilitate repeat experience.

But in academia, this is not the case.

Except for some small labs or individuals who have been working on some kind of project for many years, the requirements for the expansion of academia, or in other words, the requirements for opening up new fields are much higher.

Therefore, most academic researchers are actually in a state of lifelong learning and constantly trying new projects and new jobs.

This is especially true in the more advanced laboratories, which are always cutting-edge science, but always have some new things, and among these things, there are inevitably familiar ones, but there are inevitably obscure ones.

When you encounter a strange situation, you learn to do it, read books or do experimental training on your own, and finally you are familiar with it, and a project may end, and then repeat it again.

Su Xiankai has never done bovine rhodopsin protein before, there are not many in the world, if you want to study, there is no place to study, it is good to teach two or three kinds of protein in school, if you teach each of them, not to mention whether the textbook can be compiled, there is no lifespan of eight hundred years, this kind of thing does not need to be considered.

However, it is most common to do it without learning it, and to do it yourself.

One of the effects of repeated experiments is also the high efficiency of learning, if it is a backward follower laboratory, it is the most cost-effective to train soldiers through various repeated experiments.

Of course, pondering and learning also have a direction.

If there is no restriction of Yang Rui, Su Xiankai is more inclined to study the in-depth study of bovine rhodopsin protein itself to understand its working mechanism and function.

However, Yang Rui's orders were simply tiresome.

It's always a simple increase in production.

Increase production when it doubles.

Increase production when it is five times.

Ten times over, the order turned out to be an increase in production.

Su Xiankai's research direction can only continue to increase production.

Increasing production has never been a mystery for biologists, especially for smaller units. For example, penicillin, which is often used as an example, in the early 50s, the whole of China produced tens of kilograms, so that 200,000 units of penicillin were sold for one tael of gold, and by the 80s, what was the output of penicillin in China? Hundreds of ...... The unit is tons, and the price for patients has also been reduced to one or two cents a bottle.

Without him, the production of penicillin rolls up and down.

Bovine rhodopsin protein, in essence, is no different from penicillin.

It's even very similar to the coenzyme Q10 that Yang Rui did at the beginning.

Once, when Yang Rui was in the Xibao Meat Factory, the production method of coenzyme Q10 was tissue extraction, that is, to stir and crush some pig hearts, and then use various means to extract the desired coenzyme Q10.

Later, including the Xibao Meat Factory, the program was improved, and the plant extraction method was adopted, which greatly reduced the production cost, and the subsequent semi-microbial extraction program, and even the fully synthetic extraction program, were also options that could be made with technological progress.

At present, there is only one solution for bovine rhodopsin protein.

However, compared with commodities such as coenzyme Q10, research materials such as bovine rhodopsin protein do not need to be too cost-demanding.

While asking for an increase in the supply of fresh bull's eye, Su Xiankai began to slowly change the solution used for extraction, and even took measures to extract again...... When it comes to the specifics, it is naturally very complicated, but in essence it is actually quite simple, similar to factory oil extraction, one is to increase the total amount of extract, and the other is to reduce the loss.

Moreover, just as oil extraction requires a refining process, bovine rhodopsin protein also needs to be purified.

It took Su Xiankai almost a month to finally enter the fast lane.

The increase in production has also increased from 10 times to 50 times.

In this way, the Ion Channel Laboratory of Peking University has officially entered a node - the output of raw materials has finally exceeded the consumption.

Gu Qiang, Fan Zhenlong and Wang Sisheng, plus two scientific research dogs, although they were busy, but in the end, the five of them couldn't consume so much bovine rhodopsin protein.

In this regard, Yang Rui naturally would not let it go lightly, and forcibly ordered: "Give you a buffer time this week, everyone will work overtime to use up the bovine rhodopsin protein made by Lao Su's group, and from next week, I hope you will think of a better way to speed up the experimental process." ”

The faster the experiment, the greater the consumption of raw materials, of course, there are some crooked ways, but in such a small laboratory, this is not done.

Fan Zhenlong had a bitter face, saying that the baby couldn't do it, or he would add someone to the baby.

Yang Rui said without sympathy: "The increase in manpower can only be the better performance group, the progress on your side is obviously slow, this cannot be solved by increasing manpower, on the contrary, you have to find a way to improve efficiency." ”

Gu Qiang, Fan Zhenlong and Wang Sisheng, two scientific research dogs, had to work overtime in a regular manner.

However, their overtime hours are not as good as Su Xiankai in any case.

In order to achieve 100 times the production capacity, Su Xiankai has long been freed from the 110-hour work system, he works more than 130 hours a week, and occasionally only works 15 hours a day, he will be excited and unable to sleep.

The output of raw materials came up, and the follow-up experimental team had to keep up.

The conformation of the transmembrane region of the G protein-coupled receptor has finally been browbed step by step.

To get a high-resolution 3D structure, these are the three steps.

The first step is to have a conformation.

Conformation is an exclusive term in organic chemistry, it is a high-end version of hydrogen dioxide, in space, indicating where the hydrogen is, where the oxygen is, and preferably the angle between each other.

The second step is to obtain the crystal structure.

The third step is to obtain a high-resolution 3D structure.

Any of these three steps is an S-class result that can cause a sensation.

In fact, except for Yang Rui, the goals of other international laboratories are only one step to complete.

No one wants to go through three steps at once, or even go one step further.

Because of these three steps, except for the first step, which is a little reluctant, each step is the result of a possible Nobel Prize, who would give up such a huge temptation to study the next step secretly?

However, the temptation to come to three conclusions is unquestionable.

Assuming that the Nobel Prize is awarded to the three-dimensional structure of the G protein-coupled receptor, those who are eligible to receive the award should include the completer of the crystal structure of the second step, the completer of the three-dimensional structure of the third part, and the completer of the subsequent signaling.

Of course, since the 70s, there have been fewer and fewer Nobel Prizes awarded separately, and they have even tried to combine two unrelated achievements in order to satisfy the number of three people.

The number of people who are really eligible for the Nobel Prize has increased greatly over the years.

However, G protein-coupled receptors are still a strong field, and if anyone can win the Nobel Prize alone, it can only be such a project.

Although Yang Rui knew that it was unlikely, he still didn't want to give up at the beginning stage.

After all, foreign laboratories have not yet made efforts, and the Peking University Ion Channel Laboratory still has some opportunities, even if they are small, they can still be called opportunities.

Besides, if it is very small, the idea of making a G protein-coupled receptor is itself very small, let alone the next steps.

……