"You please. Pen | fun | pavilion www. ｂｉｑｕｇｅ。 info" Director Mei let go, and felt that it was inappropriate, so he couldn't help but add, "Director Yuan, Yang Rui doesn't know that you have done scientific research, this ......"

"I understand, I understand. Director Yuan smiled with the majesty of the superior and said, "I just look at it and don't say it, let's go back, and I'll nag you again." ”

"You laughed, when the time comes, I will put on the table and invite experts to come and listen to you explain the analysis to us. Director Mei saw that Director Yuan finally knew what he was doing when he invited him, so he relaxed a little.

He wanted to know about the situation in the laboratory, but he didn't want to offend Yang Rui.

In a few moments, the two of them registered at the doorman and entered the laboratory.

Director Yuan looked around, still disgusted, and said, "This laboratory is an old factory, right?"

"It used to be a warehouse. Director Mei replied in a low voice.

Director Yuan shook his head and said: "This is not good, foreign laboratories, not to mention what advanced instruments are in them, first of all, we have to do a good job of infrastructure, I see that the paint on the top has fallen off, and no paint has fallen down, which will affect the experiment." ”

Director Mei smiled and said, "No." ”

"Why not, those who do experiments, these things have to be paid attention to. ”

"I guess I still don't have any money. ”

"Didn't you donate money to them, and where did it go?"

Director Mei was a little surprised and said, "You know this?"

"Who doesn't know. Director Yuan smiled and said: "China Animal Husbandry Corporation donated millions in one go, and the people in the circle watched it." ”

At this sentence, Director Mei was a little nervous.

He looked around and couldn't help but feel that the infrastructure was too old. However, thinking about what Yang Rui said before, Director Mei still explained on his behalf: "It is said that many steps of embryo transfer of this cow have to be done on site. The environment on the ranch is even harsher than here, so there is no need to spend a lot of money on renovating the laboratory. ”

"That's right. Director Yuan pondered for a moment, nodded slightly, but still disliked his face, and said: "Actually, in the final analysis, it is one word, poor." Our domestic conditions are poor and funds are scarce, which is an objective condition, and under the constraints of objective conditions, it is not easy to do research itself, so ...... Instead of learning to do embryo transfer in a foreign laboratory, it is better to do some simple research, don't you say?"

Director Yuan's words, the first half of which was pleasing to the ear, and the second half made Director Mei a little reluctant.

However, the two of them had already entered the laboratory, and he couldn't quarrel with Director Yuan right now, so he could only nod depressedly and said, "Here, the one on the right hand side in front is Yang Rui." ”

"Pretty young. ”

"Yes, I'm only 22 years old. ”

"At the age of 22, I already have a laboratory. Director Yuan sighed twice and said, "When I was 22 years old, I was still teaching below, and I searched all over the county, but I couldn't get a bottle of concentrated sulfuric acid." ”

Director Mei smiled and nodded again, and then greeted the back in front of him, "Director Yang, I'm here again." ”

"Yo, Director Mei. Yang Rui glanced back, saw Director Yuan, and asked with a smile, "Who is this?"

"Director Yuan is the leader of our Science and Technology Commission, I talked about it yesterday, and I came to see our laboratory today. "Director Mei tried to say it lightly.

Yang Rui smiled with some surprise: "This is a leader inspection......

After saying a few nice words, Yang Rui did not make a move to lead the inspection after all, and in the final analysis, he has no affiliation with the Commission of Science and Technology.

"Director Mei, Director Yuan will hand it over to you for hospitality. Yang Rui didn't have a deputy like Xu Zhengping in the genetic engineering laboratory, so he simply threw it back to Director Mei.

"No, I'll just take a look around and learn about your work. Director Yuan came over to see Yang Rui sit in the experiment, he found a chair and sat down, and observed.

Yang Rui was slightly puzzled, but still worked as usual.

He has already done the part of providing primers for PCR, and this work is not something that can be learned by looking at it with the naked eye.

In fact, most of the experiments are not something that can be learned by looking at them, not to mention those complex chemicals, which make it difficult to distinguish the type, content and proportion, and most of the time of primer design is based on luck.

The so-called primer is the primer of the drug in the PCR reaction.

For PCR to work, you must first use primers to lure out the desired DNA fragments.

Therefore, primer design is a critical step in successful PCR amplification, and good primers can not only avoid the generation of background and specifics, but also identify genomic templates and so on.

However, primers are artificial, just like primers for medicine, which are always hard to come by.

In the 21st century, primer design is a relatively simple task, not only with universal genes for reference, but also with a large number of design templates, and even three huge international nucleic acid databases to query the corresponding information resources.

Of course, there is also the corresponding primer design software available.

Even so, the vast majority of scholars do not design primers themselves, because the work is tedious and professional, and they prefer to find a professional company to outsource this work.

Unless they are at the forefront of the industry and are unwilling to disclose the breakthrough points and focuses they are exploring, someone will design their own primers. Of course, there are those who can't afford it, and old-school scholars will do the same.

By 85 years, Yang Rui naturally couldn't find primers that could be used.

In fact, he couldn't find almost anything, let alone primer design software or the corresponding nucleic acid database, even the simplest general template, he couldn't even think of getting one.

At present, PCR research is in full swing - naturally it is not the competition for PCR itself, but the competition for the scope of the use of PCR.

It's as if when X-ray first appeared, a large number of scientists started from their own research fields and thought of ways to use it, so everyone rushed to do it, at the very least, to make the simple part first.

There's no easier way to brush your face and prestige and impact factor than this.

In this state of fierce competition, you can't expect well-meaning scholars to publish primer templates that they've worked so hard to make. If there is, it is usually an incomplete part.

Fortunately, Yang Rui himself has designed primers, and with the support of various literatures, he is qualified to do it.

Of course, during the period of designing PCR, Yang Rui had to make some primer templates, but he couldn't use them on the topic of gender identification.

He even had to start with the genetic sequence of the cow.

This actually involves the genome project. The goal of the Human Genome Project is to sequence every gene in the human genome and mark it like a map, and part of the role is to make it easier to run PCR.

And in 85, there was neither a human genome project nor a cattle genome project.

Any unit or individual who wants to know which segment of the gene will sequence it by himself.

In fact, this is not a particularly difficult thing, the human genome is composed of 3 billion base pairs, so it took a lot of time, manpower and money, and the core sequence of a specific gene of the sry sequence that Yang Rui needs, even if it is required in the 80s, only costs tens of thousands of yuan.

Of course, there are very few laboratories that can do gene sequencing now, and in China, Yang Rui's ion channel laboratory is also counted.

The ion channel laboratory has set up a special research group for a long time to do a lot of research related to genes, and it is not very difficult for them to set up a separate project and do a special gene sequencing.

If other laboratories have requirements, it is natural to negotiate and arrange time, Yang Rui has requirements, as long as the corresponding funds of the genetic engineering laboratory are allocated to the ion channel laboratory, it is easy to get the results.

It was on this basis that Yang Rui began the design of primers.

I saw that he spent most of his time doing paper work, and a small part of his time dealing with bottles and cans.

Director Mei was dizzy when he watched, and Director Yuan was also sweating on the top of his head.

Director Yuan knows the PCR machine and understands the principle of PCR, and he has also used the PCR machine, but it stops there.

Director Yuan came back from the UK more than a year ago, and at that time, PCR was only made by Yang Rui, so Director Yuan did not have contact with PCR and its use abroad.

Back in China, Director Yuan has not been working in the front line for a long time, although he maintains the tradition of reading journals and often going to the front line, but he really does not know much about the emerging PCR.

Of course, if Yang Rui uses PCR normally, it is not difficult for Director Yuan, it is nothing more troublesome than an X-ray machine, and it is not more difficult for researchers to operate than a rice cooker.

However, when Yang Rui began to design primers, Director Yuan was blind.

Most scholars who know in general terms are blind.

And under Director Mei's expectant eyes, Director Yuan was even more embarrassed.

"Let's go back. At noon, Director Yuan couldn't wait to walk out of the laboratory.

Director Mei hurriedly followed, and asked in a low voice, "How are you seeing? Are they getting to fruition?"

Director Yuan was silent, looking thoughtful.

(To be continued.) )