From Wang Liangcai's point of view, Yang Rui's operation is difficult to say.

He was a student of Tong's lineage, studied genetics as a child, and trained with scholars from the Institute of Hydrobiology of the Chinese Academy of Sciences for many years. In terms of egg peeling technology, Wang Liang is world-class, far surpassing the so-called egg peeling master of Peking University, and Yang Rui's technology is not in his eyes.

However, Wang Liangcai was eager to record every action of Yang Rui's operation at this time.

In just a few tens of minutes - almost equivalent to the time to wake up from watching Wong Kar-wai's movie in the cinema, Yang Rui has basically completed the operation of corring.

It is not difficult to denuclearize, especially for researchers from fisheries research institutes.

But a player like Yang Rui only failed once, and successfully completed the coring operation, which is really surprising.

In other words, Yang Rui's operation method is not worth mentioning, but he used such an operation to easily complete the nuclear operation, which is the reason why Wang Liangcai is concerned.

In the cellular manipulation of cloning, a total of two enucleation operations are required.

The first enucleation operation is to remove the nucleus of the oocyte of the sheep. Because this nucleus is not used in the later stage, the first point of the enucleation operation is to suck out as little cytoplasm as possible, and at the same time, to suck the nucleus clean.

The second enucleation is for ordinary cells.

The cloning of sheep is used in the mammary gland cells of sheep, and the key point of operation at this time is to completely suck out the cytoplasm of the mammary cells of the sheep, as for the mammary cells themselves, because they are also abandoned in the later stage, so it does not matter.

In general, both steps are actually refined, and there are very few shortcomings that can be tolerated. The best state must be to completely separate the nucleus from the cell itself.

Otherwise, if the nucleus left by the oocyte is involved in the development in the later stage, it is difficult to say whose sheep will be born.

If it is used for cloning, you originally wanted to clone Dolly the sheep, but it turned out that Dolly carried the genes of Dolly, and this kind of cloning is not clear.

It's not a sensational clone either.

From the very beginning, Wang Liangcai was very clear about the purpose of this part of the operation, but in order to suck up the nucleus of the oocyte, he had to suck out more cytoplasm, and the remaining cytoplasm was not enough to develop, so that it failed.

In fact, Gu Qiang's operation is the same, in this step of operation, the cytoplasm is too easy to be sucked out.

On the contrary, it is the second step of operation, which does not matter how much cytosol is lost, only the integrity of the nucleus is required, and the technical requirements for the experimenter are much lower.

Yang Rui's newly developed technology is different.

Although the goal of the operation has not changed, it is still necessary to aspirate all the nuclei and as little cytoplasm as possible.

However, Yang Rui's operation lowered the requirements a little, not only did he not pursue a complete sucking out of the nucleus, but deliberately sucked up the nucleus, and then sucked out the fragments little by little.

Aspiration of the nucleus intact can certainly meet the requirements, but it is too difficult.

Moreover, the nucleus is not a hard solid, it is more like a balloon swimming in a viscous liquid, if it is completely sucked out, it is inevitable to increase the suction, so as to suck out more cytoplasm, in addition, when the balloon comes out, it is always inevitable to bring out more cytoplasm, sometimes bring out more, directly declare the experiment failed, sometimes bring out less, and there is a lot of luck.

If you suck it up, you will certainly cause more trouble.

Think about a balloon that shatters in the liquid, and the gas in the balloon floats out, forming bubbles in the liquid, and sucking them out one by one, how troublesome it is.

However, trouble is a trouble, but it is a troublesome job that can be done, and in addition to the careful and tedious operation, it is much easier to suck up the debris than to suck the whole balloon.

In some cases, if the nuclear needle is well controlled, there is no need to suck many fragments, and the task can be completed after three or five negative pressure suctions.

After Wang Liangcai discovered this, he couldn't walk anymore.

Immediately afterwards, Wang Liangcai discovered that sucking and crushing was only the smallest point in the new technology developed by Yang Rui.

The use of the spindle to control the amount of absorption is the most powerful part, and it can be said to be exquisite.

As the name suggests, the nucleus needle is used to suck the nucleus, but how small is its tip? Basically, you can't necessarily see it clearly under an ordinary magnification microscope.

Therefore, when operating the aspiration needle, the slightest tremor will cause it to swing violently.

You can imagine what it feels like to thread a needle with a rope in your hand. The cells are much smaller than the eye of a needle, and the hands tremble a lot, not to mention sucking the nucleus, and it is normal to poke the cells.

Yang Rui's level is weaker than Wang Liangcai, and he is also an experimenter with a certain level, but he still accidentally punctured an oocyte, which can measure the difficulties.

However, when the needle is pressed against the spindle, the flutter disappears naturally.

It's like holding a long stick in your hand, the head of the stick can't help but tremble, but when you poke the stick against the wall, the head of the stick won't tremble.

In addition to being able to control the jitter of the needle and increase accuracy, another benefit is that it controls the front and back flutter of the needle.

It is equivalent to saying that when the nuclear suction needle pokes the spindle, it seems to add a fault-tolerant device out of thin air, making the operation easier all of a sudden.

It's easy to say, but it's not easy to think of using this trick.

The most difficult part is that you find the right poke position.

The spindle is also a very important part of the cell, and it is not something that can be poked if you want to. It is naturally tough, but some parts are too tough to hinder absorption, and some parts are unexpectedly fragile and can be easily punctured.

The rest of the spindle that can be poked is best in a relatively easy to operate part.

Wang Liangcai watched Yang Rui's movements, his mind turned a thousand times, and he couldn't wait to master the new method immediately, and try it when he turned around.

At this point, Yang Rui could not meet Wang Liangcai's requirements.

He is already slow, and he has only tried a few new methods, and the operation is so clumsy that people want to jump their feet, like a child who has just learned to shoot a ball to make a three-step layup, and when he doesn't foul, he is ugly to death, not to mention that he has more fouls than he walks.

"Professor Yang, I kind of understand it, otherwise, I'll try it?" Wang Liangcai whispered.

Yang Rui raised his eyelids and said, "What, do you think my operation can't work?"

Wang Liangcai coughed twice, and said against his will: "How can it, you have no problem operating." ”

"I also think, wait a minute, I'll finish this nucleation. Yang Rui really felt that he was doing a good job, but it was not as good as Wang Liangcai's operation, and it was still better than the previous operation.

Naturally, he ignored the difficulty gap between the old and the new.

Wang Liangcai was in a hurry, and he couldn't urge, so he could only watch obediently.

If Wang Liangcai had been given two catties of bear cell nuclei, he would probably have jumped up a long time ago to tell the truth, and the same conditions would also apply to other researchers in the laboratory.

As time passed, Su Fan, Gu Qiang, Tian Bing and others silently gathered in front of the experimental table.

What Yang Rui's experimental ability is, everyone has some numbers in their hearts.

And how long an oocyte can withstand the ravages of everyone, everyone has some numbers in their hearts.

Normally, if you give Yang Rui an oocyte, he just won't puncture it, and he won't be able to play for two hours.

No matter how long it takes, the progress of the experiment will be deeper.

Thinking about Gu Qiang's luck, Wang Liangcai slept in the laboratory for several weeks before he barely caught up with the progress...... Yang Rui couldn't have taken Gu Qiang's luck and did it for a few hours in one go.

The more researchers you follow, the more curious the other researchers become.

In the afternoon, the experimental table where Yang Rui was located was already surrounded by people.

Yang Rui didn't notice anything.

It's not like other people are experimenting with boring things, he's trying new methods, it's kind of like playing a new game, and he always wants to stop at first.

Goo.

Someone's stomach rang.

Yang Rui raised his head blankly, rubbed his sour neck, and only then did he notice all kinds of strange eyes around him, as well as moving human heads.

"Hey, don't crowd it all, isn't it hot?" Yang Rui helplessly drove everyone away: "Be careful, don't pollute the experimental table." ”

Everyone hurriedly took two steps back to make way for space.

"It's almost eight o'clock, let's go eat if you haven't eaten, I still have a few hours on my side." Yang Rui touched his stomach and decided to wait before eating, his stomach could wait, but his cells couldn't.

Everyone nodded and persuaded each other: "Lao Tian, you have a bad stomach, hurry up and eat." ”

"Xiao Lei, don't rely on it, go to eat and eat. ”

"Su Fan, today there is your favorite braised pork, fast hands and slow hands. ”

"Then why don't you go? Su Fan pursed his lips, not caring about the persuasion of others.

In fact, no one wants to leave at all.

How rare is the opportunity to see the demonstration of the new technology, or the Nobel Prize-level Yang Rui is demonstrating, and in the future, he said, "When I watched Professor Yang do egg cell enucleation", the party naturally got up.

Yang Rui didn't say much, he was originally demonstrating technology, so he couldn't drive people away.

He touched his stomach and continued to do it, and the others gathered together as usual, watching and discussing in a whisper.

In a laboratory, there is always a distinction between high and low levels, especially when facing a specific topic, there are always people who have a thorough grasp and there are always people who are not very good at it.

At this time, everyone's focus is different.

The so-called layman looks at the lively, and the insider looks at the doorway, and it is the same in the same laboratory.

Even if you just look at everyone's record book, Wang Liangcai remembers more than others.

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