Both Nan Zhiqiao and Li Guoyi suspect that the virus isolated from Ellus's body is a new filovirus.

To be sure of this idea, it is necessary to have evidence.

To identify whether the virus is a new unknown virus, Nan Zhiqiao gave a set of identification schemes.

First, the virus was isolated, the virions were purified, and the morphology of the virions was observed under an electron microscope, and the viruses were classified by appearance.

Second, animals are infected with isolated viruses, multiple antibodies are made, and whether there is immune cross-reactivity with known viruses.

Then, the phylogenetic tree was drawn using molecular biology methods, protein N-terminal sequencing, sequence comparison, specific PCR primer design, PCR, sequencing, nucleic acid sequence comparison, and molecular biology methods.

Finally, animal experiments to determine the pathogenic mechanism of the virus and sensitive drugs.

A complete set of identification plans came out, and Li Guoyi's experimental progress of Guojing Medical Center only completed the first step, isolating and purifying the virus, observing it under an electron microscope, and determining it as a filovirus genus according to the appearance of the virus.

The next step is to determine if it is a new species of filovirus.

Li Guoyi used the virus isolated from Ellus, inoculated experimental rabbits, and prepared to make polyantibodies against the new virus, which were used to detect whether there was cross-immune reaction with the other two viruses of the genus Filovirus.

The preparation of polyclonal antibodies takes a period of time, and at the same time, Li Guoyi also carried out the third step, sequencing and sequence analysis using molecular biology methods.

Nan Zhiqiao is in Liberty City, Sierra Leone, and usually has his own assistance mission work, and in his free time, he guides Li Guoyi from a distance, and at the same time he also designs several PCR primers.

Just when Nan Zhiqiao was helping Li Guoyi in China design the specific PCR primers for the new virus, Dr. Sky came with new news.

Another patient with symptoms similar to Aurus has appeared in the Kenema Government Hospital.

The patient's name is Kay, a local black man, and he was sent to Kenema Government Hospital by his family this morning, and when he was admitted, the patient was already in shock, bruised all over his body, and bleeding from every hole in his body.

Dr. Sky sends a photo of the death of the patient, Kay, to Nan no Joe.

The patient's death in the photo is much more terrifying than Dr. Sky's description, with blood-stained sheets, naked body, swollen and blackened**,** dripping with blood, and bloodshot eyes full of bloodshots, like two purple-black grapes embedded in the eye sockets.

When Nan Zhiqiao looked at the photo sent by Dr. Sky, Ye Zhenzhen was beside Nan Zhiqiao.

Ye Zhenzhen looked at the photo in her eyes, thought of the previous death of Elous, compared the two, and said: "The symptoms are very similar, could it be caused by the same pathogen?

To find the connection between the most recent deceased and Elous, it is necessary to find out the identity of the deceased Kay.

Repeating Kay's name, Vera felt familiar, "The information about the Irus stream sent by Dr. Sky before, which mentioned that the name of the owner of the North Eagle Lumber Mill is Kay, can't it be this person?"

To confirm this, Nan Zhiqiao took the initiative to call Dr. Sky.

After Dr. Sky's investigation, it turned out that the deceased, Kay, was the owner of the North Eagle Lumber Mill and the owner of the monkey breeding farm in Case Town.

Irus had sent two monkeys from the forest to the monkey breeding farm managed by Kay half a month ago.

This is the only connection between them in the recent past.

Finding the point of contact, the monkey, Nan no Qiao suggests that Dr. Sky investigate the monkey breeding farm managed by the deceased Kay.

Suddenly two patients are in a miserable state of death, and the doctors want to find the cause as soon as possible, Dr. Sky listens to Nan no Qiao's advice and goes to the monkey breeding farm in the small town of Case the next day.

——

The monkey breeding farm is located on the north side of the small town of Case, next to the North Eagle Lumber Mill, in a dense jungle, in front of several dilapidated wooden houses, hangs a rusty iron plate with the words "Monkey House" written on it in red paint.

The monkey breeding farm, which can also be called a monkey farm, or a monkey house, is very simple and does not even have a decent name.

There are several rows of cages in the cabin, most of which are empty, some with monkeys.

These monkeys are caught by local villagers from the forest and sold here.

Kay bids are low, ranging from a dozen dollars to tens of dollars per monkey, depending on the size and fitness of the monkeys.

Locals don't like wild monkeys and feel that they often come to the farmland to steal food, and the villagers are happy to sell the monkeys they catch here.

Kay has hired a man to take care of the monkey farm, and this person is responsible for feeding the monkeys, cleaning up the droppings, and cleaning up the surroundings.

When Dr. Sky arrived at the monkey farm, the hired worker was sorting out the fruit for the monkeys in the yard of the monkey farm, and he was a black man in his fifties, very thin.

Dr. Sky identifies himself and explains his intentions, and Kumo, an old black man, is happy to answer Dr. Sky's questions.

When Kumo, an old black man, learns that his boss has died, he is surprised and worried, not because he will get sick, but because he will not be paid for the rest of his salary.

Dr. Sky asked about the current situation of the boss, Kay, and the monkey farm, and Kumo, the old black man, replied in detail.

Kumoer, an old black man, told Dr. Sky that he had just come here ten days ago, and before he came, there was another cleaner in the monkey farm, kupper, who was also a nearby villager, who had taken a few days off intermittently because he was sick, and then he became seriously ill and never came to work, so the boss Kay found someone to help again.

Hearing that the original cleaner was sick, Dr. Sky became alarmed and asked what was wrong with the original cleaner, why was he sick, and where did he live now?

Kumo, an old black man, said: "I don't know what disease he had, but I heard the boss Kay complain that the original cleaner was bitten on the arm by a monkey while cleaning monkey droppings, went to the town clinic for two stitches, and took two days off, and then when he came back to work at the monkey farm, he was always listless, and after a few days, he was really sick and went home, and since then he has not returned to the monkey farm, and he should now be at home in a nearby village." ”

Monkey bite, sick, listless...... These key words made Dr. Sky faintly aware of something.

Dr. Sky: "Where is the monkey that bites someone?"

"Dead, buried in the woods not far away. ”

"Dead?" Dr. Sky had a bad premonition, "how did that monkey die?"

"I heard from the boss that after the monkey bites, he is very short-tempered, and he dies of illness two days later, and all the monkeys that die of illness will be buried in that forest. ”

The monkey turned out to be sick and died!

Dr. Sky is now worried about the original cleaner, how sick he is, and the other monkeys in the monkey farm, will they be as sick as the monkey before him?