Within a few days, the paper sent by Yang Rui to "JMC" was included, and the other party used the telegram intimately, first notified the inclusion, and then sent the official letter by mail. Pen "Fun" Pavilion www.biquge.info

Yang Rui is the corresponding author, so naturally he came to read the letter and pass it on to everyone to boost morale.

Huang Mao praised: "The big magazine is good, first telegraph and then mail, we will feel much more at ease here." ”

Li Wenqiang said: "It's just that there are more review procedures, which is too troublesome, but our publication this time is quite fast." ”

"Because it's Yang Rui's name. Wang Xiaoyun sighed twice and said, "Yang Rui is now registered on someone's side." ”

"I wrote a special letter with sincere words. Yang Rui laughed twice and said: "What JMC needs most is the author with a high number of submissions, and I promised the other party that I will submit more than three articles in the next month, and everyone has to work hard." ”

"Submit three papers, so much?" Zhang Xuetong, who has been squatting in front of the DNA synthesizer for a long time, feels that it is a little difficult to complete.

Yang Rui nodded and said: "We are a laboratory now, you count and see, me, Huang Mao, Tu Xian, Wang Xiaoyun, Wei Zhenxue, Li Wenqiang and Duan Bo, and you Zhang Xuetong, these are 8 full-time researchers, plus full-time assistants Wang Lei and Zhou Yuwen, if we can't write three papers overtime, I'm afraid we can't even complete thirty papers in a year, so how can we do it." Starting today, each team should complete the project in hand as soon as possible according to the assignment. ”

It's not a lot to complete 30 papers a year in a lab. By the standards of Yang Rui's graduate school, the provincial key laboratory in the upper middle level has at least two or three experimental groups, and each experimental group will have six or seven experimental dogs and one to three full-time teachers. Experimental dogs write a paper in two years, full-time teachers write two papers a year are the minimum standards, experimental dogs who can't write a paper in two years can't graduate, full-time teachers who can't write two papers a year, can't even eat and wait for death, a full-time teacher with a little level, it's normal to write seven or eight papers a year, plus the bosses of each research group, thirty sci-level papers, it's not difficult at all, if not, where can China become a big country in papers.

With the current scale of Sinovel laboratories in China, it is actually already a first-class level, and the laboratories of Peking University Tsinghua University or the Chinese Academy of Sciences pull out, and the total amount of instruments exceeds 500,000 US dollars, which is only a handful.

After 30 years, a laboratory of this level cannot fit in a single building.

Zhang Xuetong used to be in a local university, with few resources and a slow pace, and when he saw Yang Rui say that there were less than 30 articles, he believed it.

Huang Mao and Tu Xian looked at each other, with a wry smile on the corner of their mouths, knowing that Yang Rui was going to urge them to work overtime again.

However, overtime work in research is common, and if you do it slowly, the results will be all someone else's.

After chatting for a while, several people were commanded by Yang Rui to enter the experimental state.

Huang Mao collected the experimental reports of the last few days and began to write a new paper - "A New Method for Improving the Yield of Oligonucleotides".

Huang Mao has been to the United States, has the highest level of English among several people, and writes English papers more smoothly than Chinese papers.

If it is a top-level paper, Huang Mao belongs to the main writer, and he can also be the first author.

Of course, his own contribution is also the contribution of the first author.

Tu Xian, Li Wenqiang and others formed their own groups, sharing full-time assistants Wang Lei and Zhou Yuwen. Wang Lei is the little fat man who has met Li Wenqiang, he and Zhou Yuwen are both young people who are not very demanding, young and strong, and they happen to be working hard in the laboratory.

On the premise of getting a supply in front of raw materials, Yang Rui began to try to copy DNA.

This is where it gets a little bit awkward.

Yang Rui had to start with the simplest part, even if he knew it was wrong.

However, the right experiment is always born out of countless mistakes, unless you are born to know, otherwise, a single attempt is not successful, and most of them will be regarded as plagiarism.

Such high-profile achievements must be well-founded.

Yang Rui prepared a separate experimental book, using a five-to-ten-cycle method, distributing the heating reaction solution, cooling it, and then adding polymerase to expand the template.

After several cycles, Yang Rui added radiolabeling, and finally, he precipitated the digested DNA sample, and then ran electrophoresis.

Rightfully there is no desired gel strip.

"PCR01" failed!

Yang Rui made a record in the experiment book, and then carried out the PCR 02 experiment without hesitation.

In the "PCR 02" experiment, Yang Rui pre-purified the template and removed the factors in the system that might hinder DNA amplification.

Obviously, this is also not enough.

As a result, "PCR02" failed.

Yang Rui recorded the reasons for the failure in the experiment book with an expressionless face, analyzed the process, and summarized the reasons......

For the next week, Yang Rui lived like this.

If there is any benefit, it is that Yang Rui has exercised his own techniques in the process of repeated experiments.

It is the laboratory of later generations, the materials are relatively rich, and the experimental dog does not have so many experiments to do.

Even if the reality is met, the psychological pressure can be unbearable.

Just like the experiment of potassium ion channel, Yang Rui did not participate too much, but handed over the experimental work to Huang Mao. Because it's a trial-and-error experiment, you don't know which one will succeed, but you don't dare to relax every time, for fear that you won't be able to answer because of a mistake, which will lead to a failure.

The psychological pressure of such an experiment is extremely high.

On the contrary, PCR is an excellent method of experimentation in its own right, and it is not particularly difficult to operate as long as it is done correctly.

Yang Rui knew where he was wrong, so he didn't have any psychological pressure, just kept repeating it and accumulating failure experience.

In the eyes of others in the laboratory, such Yang Rui is a bit terrifying.

Doing experiments with a blank face, doing experiments over and over again, doing mistakes again and again, doing mistakes again and again, and without any frustration.

This made Huang Mao and others extremely difficult to understand.

I was also very worried.

Therefore, when the school came to hold a small meeting in the second week, Huang Mao did not refuse on behalf of Yang Rui as before, but found Yang Rui and informed him: "The school sees that you haven't been back for a few days, and now we have a meeting to see your people." Do you need me to find you a car?"

Yang Rui also did experiments in his brain, as if he was back in graduate school, and asked, "What will?"

"Your counsellor has a meeting, presumably for final exams, maybe sports days and debates. Huang Mao is very familiar with Peking University, and said: "Student organizations such as the Calligraphy Association will also organize activities during this time. ”

"Why?"

"Because the course is almost over, and there is still a period of time before the final exam, it is a gap period...... Anyway, let's go to a meeting. The projects in the lab, we will continue to do. Huang Mao is full of persuasion.

"Okay. Yang Rui agreed without much thought, he has not published any key papers in PCR yet, and it seems that it doesn't matter if he leaves for a while.

However, back on campus, Yang Rui was also a little uncomfortable.

It's not that he doesn't adapt to the atmosphere of the campus, but the campus environment makes Yang Rui a little uncomfortable.

The laboratory is orderly and controlled, while the campus, it is free and chaotic......

Yang Rui walked unnaturally to the ion channel laboratory and did not walk in.

Lately, his contact with the ion channel lab has been on the phone, and it has gone smoothly and without him having to go there, but it has given him a sense of transition.

Yang Rui simply stood in front of him and looked at the poplar tree in a daze.

"Yang Rui, you're back!" a voice of surprise interrupted Yang Rui's meditation.

"Bai Ling?" Yang Rui turned his head slowly.

……