After a busy morning, it was already more than seven o'clock in the morning when I walked out of the laboratory, and it was just breakfast time. Pen Fun Pavilion wWw. ｂiquge。 ｉｎfo

Ye Zhenzhen just arrived in G Province Disease Control, many places are not familiar, Wang Yanan was very enthusiastic to take Ye Zhenzhen to the central canteen, and had breakfast together, Wang Yanan sighed: "We are usually most afraid of encountering an emergency epidemic, and we have to work overtime every time we encounter it, this time it's okay, the sample was delivered at five o'clock in the morning, and it used to be often one or two o'clock in the morning, and after the busy work, I didn't have to sleep all night, and your national CDC often does this, right?"

Ye Zhenzhen remembered the previous outbreak of hemorrhagic fever in the child welfare center, when she was doing experiments without sleep all night, and now she listened to Wang Yanan's complaints, and smiled empathetically, "Well, it's the same." ”

Thinking of this mission to Province G, Ye Zhenzhen took the initiative to ask: "The anthrax bacillus isolated from the deceased specimens in Baizi Village, have you done sequence analysis?"

"Well, I just did a preliminary sequence analysis yesterday, Mr. Ye, do you want to see the results?"

"Yes, at the report meeting last night, the keynote speaker said that the anthrax strain isolated this time has very low homology with the previously prevalent strain in City B, so in which endemic area was the strain with the highest homology with this strain isolated?"

Wang Yanan shook his head, "Yesterday, I did a preliminary comparison and analysis with several domestic epidemic strains, and there was no one with more than 90% homology with the strains isolated this time. ”

"What? The homology is so low! According to the current data, it can be listed as a single species. ”

"Yes, this is the strange thing about this epidemic, otherwise, our G Province CDC would not have asked for help, even if Mr. Ye didn't mention this matter just now, I would take you to see the sequence analysis chart made yesterday. ”

"Okay, go now. ”

Ye Zhenzhen hurriedly finished the rice in the bowl and urged Wang Yanan to go to the laboratory, every time she encountered this strange experimental phenomenon, Ye Zhenzhen and Nan Zhiqiao's reaction was the same, excited and excited.

Wang Yanan took Ye Zhenzhen to the computer room of the laboratory building, where all the follow-up work that needed to be analyzed for sequence analysis, establish an evolutionary tree, and gene sequencing was completed in the computer room.

The scale of the computer room for disease control in Province G is quite large, there are eight computers in a room of sixty or seventy square meters, and there is a row of filing cabinets in the west, and there is a row of desks in front of the cabinets, which is very empty.

There were already two people sitting in front of the computer in the computer room, Wang Yanan took Ye Zhenzhen into the computer room, and everyone nodded to say hello.

Wang Yanan sat down on a computer, turned on the computer, and dragged a rotating chair over for Ye Zhenzhen to sit next to.

Ye Zhenzhen sat aside, watching Wang Yanan call up the sequence diagram of Bacillus anthracis made yesterday from the computer, and after two glances, Ye Zhenzhen found a problem, a single sequence stood out from the crowd sequence, and the name of the strain was marked in English behind the sequence, Baizi anthracis, Ye Zhenzhen pointed to the sequence that protruded from the computer screen, and asked: "This is the anthrax bacillus isolated from the deceased in Baizi Village, right?"

"Yes, the following are representative sequences selected from various anthrax-endemic areas in China, and after comparative analysis, the newly isolated strain of Bacillus anthracis from Baizi Village is too low in homology with others, so the system software lists it as a separate branch. Zhou Yanan stared at the computer screen and said in a puzzled tone, "This is the strangest thing about this anthrax epidemic." ”

Ye Zhenzhen thought to herself, it can't be a new mutant strain, right? Every time a bacteria or virus undergoes a big mutation, the pathogenicity will change accordingly, and this time the four grandchildren of Baizi Village have a sudden illness and serious symptoms, probably because of the mutation of the newly emerged Bacillus anthracis.

Ye Zhenzhen said her thoughts, Zhou Yanan nodded, and explained: "I have also thought about the possibility of this mutation before, so I plan to analyze several of the virulence genes in it today. ”

Zhou Yanan began to call up the virulence genes of various Bacillus anthracis, began to compare and analyze, and said while operating the software: "These sequence analysis software used by our disease control in G Province is still recommended by Director Nan, and the analysis method is also uniformly trained by the CDC in China before.

"Well, this analysis software is now used internationally, and this method is also recognized when publishing SCI papers. ”

Ye Zhenzhen thought to herself, this set of analysis methods was originally taught by Nan Zhiqiao at the Institut Pasteur in Paris, and later came to the experimental group of Nan Zhiqiao of the CDC in China, and used it more frequently, every time she did the evolutionary tree of various viruses, Nanzhiqiao always asked to put all the representative strains isolated and sequenced in the world into the evolutionary tree, saying that it could be comprehensively analyzed and found something unexpected......

Thinking of this, Ye Zhenzhen suddenly had an idea, and excitedly said to Wang Yanan beside her: "Why hasn't the strain isolated from Baizi Village been compared with the epidemic strains in other foreign regions?"

Wang Yanan was stunned for a moment, and explained sullenly: "In the past two years, no anthrax epidemic has been found in Province G, and this time a few cases suddenly came, from the discovery to the isolation of the strain, and then to the sequencing, but in two days, I temporarily downloaded a few domestic sequences of Bacillus anthracis from Gene Bank, and hurriedly did a comparison ......"

I understand, it's just that time is in a hurry, and I haven't had time to do it, Ye Zhenzhen finally found a place that can help, "I'll download the global Bacillus anthracis sequence and do a comparative analysis of the whole sequence, which computer can use it?"

"Both of these can be used, and you can download sequences from the Internet, and you can also install sequence analysis software. Zhou Yanan pointed to the two computers beside him.

Vera Ye chose one, started working, and entered Gene Bank to select Bacillus anthracis first, and then start downloading by region.

As soon as the two sequences were downloaded, in the silent computer room, there was a sudden harsh ringtone of the mobile phone, Ye Zhenzhen was startled, and when she looked back, Zhou Yanan had already connected the mobile phone.

Listening to the news from the mobile phone, Zhou Yanan's face became more and more heavy, and dozens of seconds later, Zhou Yanan stood up and said to Ye Zhenzhen: "Teacher Ye, there is another suspected anthrax epidemic, a family of six died, it was just found this morning, and the specimen is on the way to be sent, I will go to the laboratory to prepare it first, and I will hand it over to you here." ”

"Okay, you go get busy, I'll leave the virulence gene and full-sequence analysis comparison here. Ye Zhenzhen happily agreed.

Ye Zhenzhen was very curious about the suspected anthrax epidemic that suddenly killed six people, and before Zhou Yanan walked out of the computer room, Ye Zhenzhen asked: "Is this new death case also in Baizi Village?"

Zhou Yanan stopped, turned around and said, "No, but it's very close to Baizi Village, and it's a village next door to Baizi Village, which seems to be called Paolou Village, and I don't know the specific situation." ”