"Director Yang, what's wrong?" Gu Qiang scratched his head. Pen, fun, and www.biquge.info

"It's just because it's so appropriate. Yang Rui muttered to himself, "You formed the complex in the presence of detergents?"

"Yes, the ARGS complex you named, which I have tested, is able to effectively couple in the phospholipid bilayer. ”

Yang Rui glanced up at Gu Qiang, and then asked, "Didn't there a decrease in coupling efficiency?" I mean, in the presence of detergents. ”

In the first phase of the study, detergents were also used to deal with G protein-coupled receptors, but unlike the second stage, in order to ensure the "purity" of G protein-coupled receptors, the second stage always requires the existence of detergents, which is a higher requirement, and the most important thing is that once there is a detergent, the coupling efficiency of G protein-coupled receptors will be extremely reduced, and it will drop to an outrageous level.

There are two ways to solve this problem, one is to complete a series of experiments before the coupling efficiency drops to unacceptable, and the other is to solve the problem of the conjugation efficiency dropping, or at least to extend the time of high efficiency.

Yang Rui asked for Gu Qiang's experimental records and checked them.

I saw Gu Qiang's experimental record, and the record in this part is only a short page.

Gu Qiang's answer was even simpler: "There was a decrease in the initial stage, but after I changed the solution, the problem of the decrease in coupling efficiency was solved." ”

Yang Rui's voice trembled, pointed to Gu Qiang's experimental records, and said, "You changed the dodecyl maltoside solution." ”

"Yes. However, it later turned out that the complex thus formed was unstable. ”

"yes, it's definitely unstable. ”

Gu Qiang nodded and said, "Then I remembered that I did the experiment before, and I encountered similar situations that were solved by enzymes, so I added adenosine triphosphate double phosphatase, which just hydrolyzed the GDP on the complex to avoid interaction with GS." ”

"yes, just hydrolyzed. Yang Rui was speechless.

Gu Qiang said with a little weakness: "Is there something wrong?" I did it all according to your plan, and I didn't derive any branches. ”

"The problem is that nothing has gone wrong anywhere. Yang Rui stared at Gu Qiang for a long time and said, "Why did you do the plan smoothly?"

"Isn't it good to be obedient?" Gu Qiang is a somewhat unruly character, but he has long been tamed by Yang Rui, and he is a little nervous at this time.

Yang Rui recalled Gu Qiang's anti-intuitive development of the "one-pot stirring" technology, and his accurate experimental skills, and suddenly asked, "Have you brushed it in your circle of friends?"

Gu Qiang was stunned for a moment and said, "I don't have any friends to trick me." ”

"Okay. Yang Rui sighed and said, "Congratulations, another top-level paper has been made from your hands." ”

"Make it?" Gu Qiang was stunned.

"Yes, it's done. Yang Rui confirmed.

"Really?"

"It's really done. ”

"No way, it's only been a few days. Gu Qiang jumped to his feet excitedly, and his cheeks were swollen and red.

Yang Rui nodded approvingly and said, "I don't think it's possible, it's only been a few days." ”

"The second phase is complete?"

"That's how the crystal structure of the complex works?"

"The crystal structure is made?"

Affected by Gu Qiang's cheers, a group of researchers gathered around.

Yang Rui is like a tour guide, telling a passionate story plainly, saying: "Although some mathematical calculations and verification are still needed, the crystal structure is almost made." ”

A group of people looked at each other and finally came down to one question: "How is that possible?"

Yang Rui sighed empathetically, not knowing how to explain.

To say that this story is destined to become a strange anecdote, it is also inseparable from Yang Rui, it is he who gave the cheats for customs clearance and gave a full set of plans.

Yang Rui also did this to increase the speed and complete the second phase of the study as quickly as possible. Given a full set of solutions, the researchers will be able to make targeted modifications and tests.

However, when given a full set of plans, it took someone a few days to complete the plan, which is the most incredible part.

Not to mention a Nobel Prize-level scientific research experiment, it is a junior high school experiment, and the teacher still gives a full set of experimental plans, can all students make results?

Find a middle school chemistry teacher to answer this question, and he can name a hundred strange experimental results, none of which are really experimental results.

How many students were able to produce results in a slightly more difficult university experiment? One out of three students was a student who was studying hard.

In weaker universities, it is common for classes to take an organic chemistry laboratory class and not produce a single result.

Not to mention the difficulty of experimenting with the crystal structure of G protein-coupled receptors, there are several major key points, first of all, using the right detergent and controlling relatively appropriate concentrations - the requirements are relatively broad and easy to achieve, but this alone is not something that can be done by a random experimental dog.

Next, complex crystals of G protein-coupled receptors are generated at appropriate concentrations.

There are even fewer researchers who can make this step, and if it were a graduation requirement for university laboratory courses, 90% of college undergraduates would have to cry.

Then, in the presence of detergents, it is another requirement to ensure the coupling of the composite crystals.

Historically, Kobika discovered that dodecyl maltoside solutions could solve the problem of coupling, and he didn't keep track of how long it took him to do so, but with a lot of malicious speculation, it was trivial to try three or four years. Of course, it wasn't Kobika himself who tried it, he had a Ph.D. student and a research assistant, but this only shows how many excellent researchers' scientific research lives have been wasted.

Once the coupling is resolved, the remaining steps, including enzymatic hydrolysis, are not particularly time-consuming, but in terms of the normal course of history, months are always required. Yang Rui operated the golden finger, and he also reserved time according to two months.

However, all of them, all these golden fingers, the flash of genius, the painstaking efforts of hard-working scholars, under the shit luck, are as ethereal as smoke and dust.

Gu Qiang just did it step by step, and the correct steps were more than trial and error......

In Yang Rui's view, it's like you install an extremely difficult stand-alone game, find a customs clearance guide from the Internet, and then ...... Three lives cleared!

"I'll take another look. Yang Rui said and said, "Su Xiankai, Fan Zhenlong, Tian Bing, that ...... Professor Man Hai, Professor Tao Xuelin, you also add in, just follow Gu Qiang's experimental steps and do a repeat experiment. Lao Xu, you and I check the experimental records again, by the way, the experimental table is not allowed to move, the garbage is not allowed to be emptied, and the packaging of all used consumables must be recorded. ”

The researchers whose names were called gathered together.

Gu Qiang still didn't realize his strong luck today, laughed, and said, "You don't need so many people, my experiment is easy to do." ”

"Well, Gu Qiang, you open another group by yourself and do a set of repeated experiments. Yang Rui didn't dare to take it lightly, this is another Nobel Prize-level achievement, it was made in a few days, who dares to believe it?

Gu Qiang changed the experimental table indifferently, took all kinds of consumables again, made them, and said: "I don't care if you say anything else, I am the least afraid of doing experiments." ”

While speaking, Gu Qiang also hummed a song.

Only Yang Rui stared at the experimental tables in the laboratory as if he were facing a great enemy, and his mind turned.

The progress chart on the white wall is brand new, only at the bottom, two participating labs, the University of Melbourne in Australia and the Winter Institute in the United States, have been added, with progress bars of 2.7 and 1.6 respectively.

Yang Rui glanced at it, and then stopped looking at it, if the triple repeated test can prove the accuracy of the results, the progress bar of the Peking University Ion Channel Laboratory will be approved as 100 points.

……