The small courtyard, paved with bluestone bricks, and a pool of hot spring water in the middle, steaming out a little gas. Pen, fun, and www.biquge.info

The moonlight is finely shattered, falling on the hot spring water, and there seems to be a faint cyan rising.

Liu Shan seemed to be gliding, falling gently into the water, like an elf in the night.

The slender waist and proud hips draw an arc in the air, as if time has stood still.

Yang Rui was holding a bunch of things in his hand, and he hesitated slightly.

"Give me the eggs. Liu Shan propped up half of her body and beckoned from the hot spring.

"Oh. Yang Rui took two steps and handed the egg basket to Liu Shan.

At this time, he saw Liu Shan's all-black one-piece swimsuit and blushing cheeks, and he didn't know if it was from the hot springs.

"I'll make the hot spring eggs, and you'll cook the instant noodles. Liu Shan swiped twice and placed the egg at her feet.

Yang Rui raised his eyes and looked around, a little hesitant where to cook noodles.

The hot spring water can be used to boil hot spring eggs, so you can't use it to cook hot spring instant noodles.

Liu Shan looked at him, suddenly a little funny, stretched out his finger and pointed to the back door, and said, "It's tied there, you go and carry water." ”

Yang Rui suddenly realized, and went to the back door in a hazy way, and sure enough, it was a latch, and there was no lock.

When I opened the back door, the street lights on the side road were dim, and there were no people or animals. The sanatorium is dominated by veteran cadres, and the most lively time of the day is 90 o'clock in the morning, and after dinner, one goes to bed earlier than the other.

Although there are people like Yang Rui who come to play, there are not too many of them, Yang Rui gently closed the back door, returned to the room in three or two steps, and came back with boiling water and bowls, and the hot spring eggs were just cooked.

"Eat it while it's hot, I'll cook the noodles." Liu Shan soaked in the hot spring for a while, and felt tired, she leaned on the edge of the pool, tore open the instant noodles, and began to put the seasoning.

Yang Rui can also see the enchanting figure underwater.

Although I have seen countless pictures and influences, it feels extremely different to see real beauties, real swimsuit beauties, especially when the two are alone.

Yang Rui only felt that his body was extremely active, and the cells in his body seemed to be alive.

"I went into the water too. Yang Rui took off his shirt in two or two times, put on shorts, and slipped off the hot spring.

Liu Shan turned her back to him, unable to see her expression, but her movements stiffened for a while.

A few minutes later, the instant noodles were soaked, and the two of them lay on the edge of the hot spring and ate them side by side.

The water is warm, and the eggs boiled in the hot springs are fresh and tender.

Yang Rui is sure that this is the most delicious instant noodles he has ever tasted.

……

It was the third day since Wei Zhenxue and the others returned to the sanatorium.

The mountain roads in the Tengchong area were much more difficult to walk than they expected, and several people suffered some skin injuries to a greater or lesser extent, in addition, it was also difficult to carry nearly 100 kilograms of samples separately.

As the youngest experimental dog, Sun Ruyue played a huge role, he was like an Eskimo, carrying more than 30 kilograms of things alone, and said quite shyly: "I am used to doing farm work in my hometown." ”

"Eat a hot spring egg. Yang Rui patted Sun Ruyue's shoulder, and then raised his thumb and praised: "Well done." ”

Sun Ruyue's fatigue from being praised suddenly disappeared, and he instantly changed from a husky dog to a Samoyed, and said, "This is what I should do." ”

"Have a good night's rest, tomorrow will be busy. "Yang Rui used to be an experimental dog, and if he came out with his mentor, he must have to do the drudgery of taking samples.

Turning his head, Yang Rui began to examine the sample.

When the specimen is in the hot spring, it is just ordinary silt, ordinary spring water, ordinary mud, but when it becomes a specimen, its name should be preceded by the word "holy".

The specimen resembles a papal nun that cannot be defiled from the moment it is placed in the box until its owner, such as a bishop or researcher, opens it.

"I'll do a few sets of tests now, you guys will rest first, and I'll help tomorrow. Yang Rui couldn't help but start. Although the sample is properly stored, no storage device can withstand time, and the best way to store it is always to use it as soon as possible.

At the very least, Yang Rui had to make sure that the samples were not contaminated and that the thermophiles were active, otherwise, it would be troublesome to return to the capital and find that there were not enough samples.

The isolation of thermophilic bacteria is only the first small step in countless steps, thinking about the distance from PCR, Yang Rui's heart still slowly rose a sense of urgency.

Although there are countless people in later generations who have commented that the technology of PCR is not surprising - it is true, that this technology that can replicate DNA infinitely is more like an elaborate technological integration than a technological innovation, but the fact that there is no surprise does not mean that it is easy.

Biology graduates in the school have to do a graduation thesis for half a year, and many people copy the experiments in the book, but they can't do anything, and then cry like a female driver staring at the parking space on the side......

The application of PCR technology is so widespread, and it is destined to be ingenious in its design.

The dinosaurs in Jurassic Park are cloned by PCR technology, and the so-called only a little blood is needed to measure your DNA, not that DNA determination requires very little blood, in fact, there is PCR technology, which replicates the DNA contained in a little bit of blood infinitely, which is convenient for testing.

Before PCR, in other words, in 1984, DNA replication was a troublesome thing.

PCR technology is revolutionary in terms of application.

It took more than three years for the original author, Muris, to be a slacker, but sometimes hardworking, but that was without competition.

What would he think if he knew that Yang Rui had similar research?

Will it speed things up?

Or give up.

Yang Rui didn't know that he had to prepare for the worst.

As for not doing PCR, don't cut off the beard, Yang Rui will not consider such a choice at all.

If from the perspective of personal interests, if you don't realize the technology with the Nobel Prize, isn't it stupid?

If we take the moral high ground as an example, achieving PCR one or two years ahead of schedule will bring countless miracles of life. Biology is not a crude art of farming, mining, or machinery, it is a discipline that is directly related to life. Realizing an important biological technology a year earlier means that countless new drugs could be invented a year earlier, that countless patients could be saved a year earlier, and that countless people's lives could be turned upside down......

Of course, Yang Rui never demanded others or his own with a high degree of morality.

However, he has more or less the feeling of "being ordered by heaven", and when it comes to doing the right thing, his spirit is extremely focused.

Liu Shan looked at Yang Rui quietly for a while from a distance of a few meters, then smiled, rolled up her sleeves, and joined the ranks of experimental assistants.

Wei Zhenxue looked at it, and he didn't rest, and said, "We also help." ”

"You go to rest, so is Xiao Sun, all go to rest, tomorrow you will be busy. Yang Rui shook his hand, lowered his head to take the sample, and said: "I don't play polite in the laboratory, you all go to rest, I'll do a few sets of experiments to try." ”

Wei Zhenxue hesitated for a moment, looking around at the blue brick walls, not sure if this was a laboratory.

However, after all, it has to be counted.

Three hours later, Yang Rui found the first thermophilic bacteria.

Although I don't know if it's the variety I want, this at least proves that there is no problem with the sample.

Yang Rui was relieved and immediately went back to his room to sleep.

You have to have a lot of energy to complete tomorrow's experiments.

The biggest difference between doing experiments and doing handicraft classes is that thinking in experiments is essential.

The handicraft class is to do the steps designed by others, at most to do the middle, and when you are unsure, ask, "Hey, where did I just stick to?"

There are many questions about experiments, especially exploratory experiments, and all the experience of predecessors can only be used as a reference.

For example, if the thermophiles in the hot springs are archaea from the age of the dinosaurs, then studying them is not much different from the research steps of a time shuttle plane arriving at the age of dinosaurs and scooping up a puddle of water.

In fact, studying thermophiles in hot springs is a bit more complicated, as it's uncertain that they really came from the age of the dinosaurs.

To do such an exploratory experiment, thinking at any time is a must in the experiment, and thinking at any time means burning the brain at any time.

In the next few days, everyone in Sinovel Labs spent their brains burning.

Yang Rui got up every day and had breakfast, and began to play with the centrifuge, except for soaking in the hot springs in the afternoon, he completely demanded himself according to the standards of the laboratory.

Kang Hong came to visit, and when he saw that their work was so nervous, he was embarrassed to stay longer, so he stayed for a long time and returned to Kunming.

Yang Rui didn't actually notice how long he stayed, his head was full of the speed of the centrifuge and the temperature of the culture medium.

The processed material obtained from the centrifuge is enriched bacteria, and it is not known how many are in one tube, which are directly applied to the solid medium and cultured in incubators at 80 and 85 degrees respectively.

The common miscellaneous bacteria that cannot survive in this environment will die naturally, and the ones that can survive will grow into colonies, and they must be cultured separately, and finally a single colony will be selected for culture, so as to realize the isolation and purification of strains.

In short, Yang Rui and the others got bacteria below their great-grandchildren, and the bacteria themselves just rolled on the culture medium, exerted their reproductive ability, and passed away with the wind.

And in the process, a lot of resources are consumed.

Bacterial medium can be more expensive than pure broth!

The medium on each plate is a strong smell of banknotes.

And the great-grandchildren of these bacteria will continue to consume resources to undergo brutal live experiments, first with Gram staining, then with whole protein analysis, and then by boiling the bacteria and splitting them for gel electrophoresis.

Once the electrophoretic pattern is obtained, four or five more steps are required to finally identify the thermophiles.

It sounds very complicated, and it's actually very complicated, but in terms of the whole experiment, it's a very simple job.

Moreover, it is also easy to make grades.

After Yang Rui determined the proximity of some strains according to the differences in protein fingerprints and compared them with the information in his mind, Yang Rui wrote the name of his new paper on the return plane: "The Discovery of the Extreme Thermophilic Bacteria with the Highest Temperature Tolerance in China".

He hadn't actually identified Tengchong thermophiles yet, but he didn't want to wait.

If the publication of Cell goes well, this paper will just start a series of letters that will keep the heat of Sinovel Labs.

It is also useful for research institutions to maintain the heat, and those laboratories that can update their research results at a fixed time will always be able to receive more attention from their peers, so that they can spread their research results more quickly, and then get more reputation and more funding.

For Yang Rui, who is rushing to a world-class project, every trace of attention cannot be ignored.

……