"Recombinant DNA methodology, which led to the development of DNA or gene cloning, by the end of the 70s, when the method of inserting short DNA fragments into a plasmid or viral chromosome and then replicating them in bacteria or eukaryotic dead pigs was relatively mature, I will elaborate on how to implement gene cloning and the reasons for the breakthrough in molecular biology that this technology has caused. Pen Fun Pavilion www.biquge.info"

Yang Rui said this, paused for a moment, and said: "A previous study I did to study the structure of potassium ion channels by cloning mutant genes was designed based on this. ”

The experts and professors who were originally a little nervous were all silent.

The publication of domestic scholars in "Cell" is extremely rare in the whole country, and Yang Rui's paper on potassium ion channels is now known to everyone in the domestic biology community.

If such a paper falls to a middle-aged scholar, it will be worshiped as a legend.

Throwing it to an academician is equivalent to brushing a new layer of gold.

Yang Rui wrote this "cell", which naturally had a stronger impact, but Yang Rui's identity slightly delayed its power, so that it did not explode, but slowly spread its power.

However, the classroom has always been a place where teachers can be at full power.

This is true for professors in the classroom, and the same is true for adjunct faculty in the classroom.

Yang Rui's thesis, at this time, is enough to deter all the bulls, ghosts, snakes, and gods.

At the same time, the academic difficulty of the paper on potassium ion channel, as a high-end application of DNA recombinant technology, is unconvincing.

The teachers who originally wanted to speak, now all lowered their heads and obediently took notes.

They are all people who have read Yang Rui's papers, and some even know about Yang Rui's scientific research competition with Richard, so no one wants to question Yang Rui in terms of DNA recombinant technology.

A cell with hundreds of thousands of words, plus a single digit icon at most, contains information that ordinary scholars can't figure out in a lifetime. When someone is the first to make a result, ordinary scholars can only bow and worship.

Scholars have been immersed in it for many years and can fully understand the difficulty of DNA recombination technology, and the application of potassium ion channel to DNA recombination technology is top-notch.

Yang Rui doesn't mention this stubble, everyone can still selectively ignore it, and Yang Rui put it forward, and then compare what he said before, no matter how strong a person is, he has to admit it in his heart at this time: Yang Rui is qualified to say that the classical genetic method is backward.

Scholars take notes and think at the same time, preoccupied with the enemy.

The atmosphere in the classroom is also more and more solemn by minute.

Although the students can't hear it, they are affected by the people around them, and they don't dare to come out.

"To put it simply, from the 60s to the 80s today, the field of genes has gone through two stages, that is, the era of classical genetic theory, and the era of recombinant DNA technology. But I would also like to introduce you today to the upcoming third era, which is the era of PCR!"

In the instant trance of everyone, Yang Rui continued: "The full name of PCR is polymerase chain reaction, the English word I wrote on the blackboard, this is a polymerase reaction invented by myself, which has been recognized and supported by peers around the world, as of last week, I published a paper on this technology in Nature, with more than 2,000 ......citations."

Saying this, Yang Rui's feeling is excellent: I created an era of biology, and that's it!

Unconvinced to argue!

No one dared to argue.

All the experts and professors, young and old, whether they looked up or down, bowed their heads again in a brief moment of amazement, amazement, and amazement.

What is the concept of more than 2000 citations?

The journal's impact factor is the total number of citations of articles in the previous two years, divided by the total number of articles in the previous two years. In other words, the impact factor is the average number of citations to an article.

And an impact factor of 1. A journal of 0 means that the average number of citations in this journal in the past two years is 1.

In the SCI index, the impact factor is 1. 0 is not the bottom line, the impact factor is 0. There are a lot of 5 journals, and these journals are good journals in terms of the journal world.

As for the impact factor of the top journals that are fascinating, it is usually more than 30 and 40 is not enough.

To put it into account, the average number of citations for papers published in Cell, Nature and Science is more than 30 and less than 40.

What kind of situation is the usual situation? This is the case of Yang Rui's paper.

As a technology destined to rewrite the history of biology and the history of human science, it is not surprising that in just a few months, 2,000 papers have been cited.

In terms of citation value, it is now equivalent to 50 S-level papers, 2,000 or even 4,000 ordinary SCI-level papers.

Even compared with the top papers of the same kind, PCR is also a record excellent.

The same super-top papers published in Nature, the higher number of citations among them, generally 200 to 300, rarely heard of 400 or even 500 citations, in the field of science, this is no longer a simple doubling problem, the world's biology laboratories are those, everyone has their own fields, not a very wide range of theories, reaching 100 citations, it is already popular all over the world.

But the end point of PCR is far from 2,000 citations.

This is the technology destined to rewrite history, and the global biological laboratories will understand it, use it, and cite it regardless of field.

Yang Rui stood on the podium with great confidence, facing the "squeaky and quack" camera, waiting for someone to debate with him.

He waited a minute.

For a long minute, there was silence in the classroom.

"Does anyone have any objections?" Yang Rui's clear voice echoed in the classroom.

With the humility of the Chinese, Yang Rui juxtaposes his PCR technology with classical genetic theory and recombinant DNA technology, calling it the third generation of technology, which should have been shouted "arrogant".

However, after a minute, no one did it.

Yang Rui waited for another minute, but still no one did.

Yang Rui sighed regretfully and even abruptly.

Although 2000 citations are enough to crush any academic enemy, it is still a bit lonely to go straight to the point where there is no enemy.

Lonely and defeated? Not yet.

After all, this is a lecture hall, and the lecturer can freely talk about his or her own opinions and perceptions, and it has no authority.

If it's an international conference, the person who raises his hand will probably break through the sky.

However, although the lecture hall did not have the solemnity of an international conference, it was still a formal occasion.

Yang Rui prefers his genomics to appear in front of the world with a more comprehensive attitude.

Writing a book and saying that it takes a book of information as thick as a book to prove itself.

"Since there is no objection, I will use this as a basis to talk about research methods dedicated to the genome. Yang Rui turned around and wrote the words "genome research methods" on the blackboard, which instantly detonated the audience.

Compared with the banner planted by Yang Rui for PCR, the direct technical guidance is obviously more exciting for scholars.

Of course, the students didn't understand what the experts and professors around them were excited about, and they looked at the blackboard blankly and bewildered.

Finally, Wang Dongming, who has always been bold and conceited, couldn't hold back anymore and raised his hand and said, "Teacher Yang, where can we see the textbook for this class?"

……