The female teacher took Ye Zhenzhen to a relatively clean test area next door, the experimental area was separated from the outside experimental area with a glass wall, there was a biological safety cabinet in the clean area, there was a refrigerator next to the biological safety cabinet, and an incubator, next to the incubator, there was a tall gas cylinder, the gas cylinder was marked with obvious carbon dioxide marking, it seems that the cells of the exam should be in this carbon dioxide incubator, there is an experimental table in the corner of the opposite wall, and an inverted microscope is placed on the tableThis is how the cell culture room in Director Zhang's laboratory is arranged. Pen fun and pavilion www.biquge.info

The female teacher pointed to the experimental area separated by a glass wall and said, "The materials for your exam are all in this room, the cells to be digested and passaged are in the incubator, the reagents required for the experiment are in the refrigerator, the examination process will be videotaped, the exam time is twenty minutes, and the timer will start now." ”

Ye Zhenzhen asked, "What are the types of cells to be digested?"

The female teacher pointed to the white paper on the experimental table next to her and said, "Regarding the experimental materials, there is a brief introduction on the paper. ”

Ye Zhenzhen walked to the table, picked up the white paper, only a few simple lines were written on the paper, and introduced the cell types and characteristics, MDCK cells, also known as dog kidney passage cells, cell passage number is the twenty-first generation.

Ye Zhenzhen thought to herself, when she was studying at Shi University in the past, she had raised insect cells, mainly the clone Sf9 of the ovarian cell line of the grass leaf moth, Sf9 is a semi-suspension cell, and MDCK cells are completely adherent cells, the two culture methods are different, but there are many similarities.

Ye Zhenzhen put on a mask and gloves, and disinfected her hands with the alcohol watering can on the table, before taking out the cell bottle from the incubator, the cell bottle is a plastic product, clean and transparent, and is the most commonly used T25 cell bottle.

The cells under the microscope, flat and regular, densely pasted all over the bottom of the bottle, but when digesting the passage, Ye Zhenzhen put the cell bottle into the biological safety cabinet that has been sterilized by ultraviolet, and then find out the required medium from the refrigerator, digest the pancreatic enzymes, and proceed step by step according to the order of operation, the whole digestion and passage process took only ten minutes, and finally, Ye Zhenzhen put the three vials of cells back into the incubator, turned on the ultraviolet lamp of the biological safety cabinet, and everything was completed perfectly.

Seeing this, the female teacher smiled and nodded, and at the same time wrote comments and scores in the notebook.

Ye Zhenzhen walked out of the cell culture room and said, "Teacher, I'm done." ”

The female teacher smiled and asked, "Yes, you have done experiments on cell culture before?"

"Well, I did. ”

"Okay, let's go back and wait for the score. ”

Ye Zhenzhen glanced at Fang Nana, who was still taking the exam next door, and was holding a pipette gun in her hand to add samples to the gel holes, it seemed that she had drawn the experiment of gel electrophoresis, this kind of experiment was too familiar to herself, but it would not end in a short time, so Ye Zhenzhen turned around and left the laboratory according to the teacher's request.

Ye Zhenzhen came out of the laboratory of the Institute of Infectious Diseases, and did not return from the original road, but followed another road, preparing to go to the agreed meeting and place on the south side of the hill in the central compound.

Out of the gate of the laboratory building of the Institute of Infectious Diseases, it is the north slope of the hill, and then walk along the cement road to the south for a distance, you can reach the agreed place.

All the way south, Ye Zhenzhen looked at the surrounding scenery, sighed in her heart, the Chinese Center for Disease Control and Prevention covers a really large area, although it is in the suburbs, not as much as the city in the inch of land, but this large piece of land is also very expensive to develop, especially, there is a mountain in the center compound, not the kind of artificial rockery that you usually see, but naturally formed, the north mountain is steep, there are dense trees growing on it, as far as the eye can see, there are more black locusts, and a few peach trees, the south side of the mountain is gentle, there is an artificially built stone road winding up, at the top of the mountain, a simple pavilion is hidden among the treesThis pavilion and stone pavement are the only man-made structures on the central hill.

Ye Zhenzhen walked south along the cement road at the bottom of the mountain, and when she went to work, there were few pedestrians on the road, but she still saw a blue and white figure walking out of the administrative building in the south from a distance.

"It can't be such a coincidence. Ye Zhenzhen was amazed in her heart, but she saw the blue and white figure getting closer and closer in her eyes, this figure was Nan Zhiqiao who appeared in her mind many times today.

Nan Zhiqiao was also stunned when he saw Ye Zhenzhen, and finally walked up to Ye Zhenzhen and asked, "Ye Zhenzhen, why are you here?"

Nan Zhiqiao in front of him was wearing a fresh blue suit, white pants, a dark T-shirt and casual shoes, and the whole person looked refreshing and sunny.

Ye Zhenzhen didn't know if it was nervousness or other reasons, her heart beat a few times inexplicably, and in the face of Nan Zhiqiao's question, Ye Zhenzhen hesitated for two seconds, "I...... I'm here to take the test. ”

"What exam?"

"This year's recruitment exam for the Chinese Center for Disease Control and Prevention. ”

Nan Zhiqiao frowned slightly and asked, "You graduated with a doctorate so soon?"

Ye Zhenzhen thought to herself, it has only been more than half a year since the last meeting, how can she complete the doctoral project in such a short time, Nan Zhiqiao's question is obviously asked knowingly, Ye Zhenzhen shook her head and explained: "I graduated with a master's degree this year. ”

Nan Zhiqiao: "Ye Zhenzhen, I remember the last time we met, you said that you didn't interrupt your studies, what happened in the past half a year?"

Listening to Nan Zhiqiao's tone with concern, Ye Zhenzhen's originally nervous mood calmed down a lot, so she replied with a smile: "Nothing happened, I just wanted to work, so I participated in this year's thesis defense and got a master's degree." ”

Obviously, Nan Zhiqiao was not satisfied with Ye Zhenzhen's answer, and then asked: "What you did in Pasteur was your doctoral project, why did you interrupt your studies in France? After returning to your home school, why didn't you study for a doctorate and get a master's degree?"

A series of questions, including the topic that was diverted by Ye Zhenzhen in the sheep ranch last year, was now asked by Nan Zhiqiao again, Ye Zhenzhen explained vaguely: "Nothing happened, I just want to graduate, besides, what is the difference between a doctor and a master's degree, it is just a diploma, and it does not affect my scientific research ability." ”

"Scientific research ability?" Nan Zhiqiao frowned and said, "Based on your previous observations, gel electrophoresis will make mistakes, such experimental skills, such scientific research capabilities, do you think you can enter the Chinese Center for Disease Control and Prevention?"

Ye Zhenzhen thought to herself, and mentioned the previous embarrassment, this Nan Zhiqiao is really chasing after his previous mistakes, Ye Zhenzhen was a little unconvinced, and said: "My test score is the first, and today's skills test should be no problem, so I am here to decide this China Center for Disease Control and Prevention." ”

Ye Zhenzhen stared at Nan Zhiqiao as she spoke, with a confident look on her face.

Nan Zhiqiao said: "Self-confidence is a good thing, but self-confidence without a reason is arrogance. ”