Old man Sun stretched out his arm to show everyone, "Look at my arms, they are all small red dots, different from the swollen lumps on Old Man Nan's head, it doesn't seem to be mosquito bites." ”

Nan Lishan: "Could it be that we buried the dead dog, they all say that after the dog dies, the parasites such as fleas and lice on the body will run out, and when we bury the dog, we accidentally let those things run onto the body?"

"What dead dog, Grandpa, where did you go in the morning?" asked Nan Zhiqiao.

"I went to a small ravine in the suburbs to dig orchid grass, it was your grandfather who heard that there were wild orchids and grass there, so he had to let me dig it with him. Nan Lishan said as he scratched the swollen lump on his forehead twice.

Old man Sun: "I have also heard people who have been to the western suburbs of the zoo say that standing on the rockery of the zoo, you can see that there are orchids and grasses on the nearby slopes, a large area, very beautiful. ”

Hearing that the place where his grandfather went was near the suburban zoo, Nan Zhiqiao immediately became alert and asked, "You went to the vicinity of the Western Suburbs Safari Park in the morning?"

Nan Lishan: "Yes, that small valley is very close to the suburban zoo, and the dead dog we found looks like a wolfdog, maybe it ran out of the zoo." ”

Thinking of Duoduo in the welfare center, because she went to a hillside picnic near the zoo, she was bitten by a tick and almost paralyzed and died, Ye Zhenzhen also became nervous in her heart, and glanced at Nan Zhiqiao, Nan Zhiqiao immediately made a decision.

The meal was almost finished, and Nan Zhiqiao pulled the two grandfathers back to Grandpa Sun's residence and examined their bodies in detail.

As expected, ticks were found in Grandpa Sun's armpits and the back of Grandpa Nan's head.

After carefully pulling out the tick with tweezers, the two old men breathed a sigh of relief, but Nan Zhiqiao was still worried.

Ticks can transmit many diseases and show symptoms after a period of time after a tick bite.

After instructing the two old people to take a few precautions, Nan Zhiqiao notified the nursing home management office and observed Grandpa Sun for a period of time.

And Grandpa Nan lives with Nan Zhiqiao, which is convenient for Nan Zhiqiao to observe the situation every day.

Ye Zhenzhen and Nan Zhiqiao, because of visiting Duoduo, but accidentally found ticks on the two grandfathers, if it is a few days later, the two old people may have the same dangerous situation as Duoduo, now thinking about it, they are a little afraid.

——

For two days over the weekend, Grandpa Nan did not show other symptoms, but the wound from the tick bite was somewhat inflamed, and the wound was simply disinfected, and it seems that there is no major problem at present.

In the new week after Children's Day, Nanzhiqiao's experimental group received a new disease control task - emergency assessment for the prevention and control of sudden acute infectious diseases.

The emergency assessment is aimed at the disease control centers of each prefecture-level city, and the assessment samples are prepared by the Nanzhiqiao experimental group, distributed to each provincial disease control, and then issued by the provincial disease control to each city disease control in the jurisdiction.

In addition to assessing the testing capabilities of each CDC, the emergency assessment also assesses the timeliness.

Nan Zhiqiao drew up the assessment project, and the blind sample preparation was handed over to Ye Zhenzhen.

Because summer is the season of high incidence of arboviral diseases, Nan Zhiqiao's proposed assessment items are as follows:

The patient is a 32-year-old male who is a foreign-aid construction worker. Returning home from a place in Southeast Asia and getting sick upon entry. The main symptoms and signs are fever with joint pain and rash. Before the onset of the disease, there was a history of mosquito bites abroad, and the clinical preliminary diagnosis was suspected of arboviral disease.

4 samples of 0.4 ml each, which were virus isolation culture medium of the case sample, which was treated by virus inactivation and contained RNA protective agent, and RNA isolation and purification were required before testing.

Testing requirements: virus nucleic acid detection, identification to species. There is no restriction on the selection of nucleic acid extraction and amplification methods, and the real-time RT-PCR method is recommended.

According to the assessment items proposed by Nan Zhiqiao, Ye Zhenzhen prepared blind samples according to the symptoms of the disease, including several common arboviruses: Zika virus, dengue fever virus, and chikungunya virus.

After the blind sample was prepared, it was transported to various provinces for disease control through a cold chain transportation company with safety qualifications, and within a week, the test results from all over the country were fed back to Nanzhiqiao's laboratory.

The first is the electronic version of the test results, followed by the paper version of the test results stamped with the official seal of the unit and sent to the laboratory.

According to the requirements for the test result report, the test report should be standardized, the information should be complete, the original record should be sufficient to reproduce the experimental process, and the nucleic acid detection record should include but is not limited to amplification curve or electropherogram, CT value, result judgment criteria and other information.

Nan Zhiqiao handed over the scoring requirements of the assessment results to Ye Zhenzhen.

For the calculation of the score of the blind sample assessment, the assessment standard is clearly explained, and the full score of the assessment is 100 points, of which the full score of the test result is 80 points, including the standardization of the test report and the original record, and the timeliness is 20 points.

In accordance with the assessment and scoring requirements, Ye Zhenzhen scored and recorded one item at a time.

There are more than 300 prefecture-level administrative divisions in the country, and there is a lot of workload for each family.

Fortunately, Ye Zhenzhen was careful and patient, and the scores and rankings of the assessment results of each family came out quickly.

The results were better than expected, with the exception of two who judged the weak positive specimens of dengue fever as negative.

After reading the results from beginning to end and making sure that there was nothing wrong, Ye Zhenzhen took the results to Nan Zhiqiao.

Regarding the results of this year's emergency blind sample assessment, Nan Zhiqiao made an expression that everything was expected, but Ye Zhenzhen was a little emotional.

"Senior Nan, this year's rankings are basically in the timeliness of the gap, and the top ones are all provinces and cities in the south, especially Guangdong. ”

Nan Zhiqiao: "Last year, when dengue fever broke out in Guangdong, they were more familiar with the emergency detection of arboviruses. ”

When it came to dengue fever, Ye Zhenzhen remembered a news she had seen on TV before, which was about genetically modified mosquitoes released at designated points in Brazil, and couldn't help but discuss with Nan Zhiqiao curiously: "Senior Nan, have you heard of genetically modified mosquitoes?"

"Yes, I've been involved in this project. Nan Zhiqiao replied lightly.

"Huh, Senior Nan, you participated in the mosquito genetic modification project!

"It was a few years ago, and the project was over, and genetic modification was one of the ways to study how to stop mosquitoes from spreading related diseases. ”

Although Ye Zhenzhen had read about genetically modified mosquitoes in the news, and Nan Zhiqiao had also participated in this project, she thought that she had read all of Nan Zhiqiao's papers before, and there was no content about this project, so she couldn't help but wonder, "But Senior Nan, there is no content about genetically modified mosquitoes in your paper, is this project carried out secretly?"

Just now, Ye Zhenzhen approached Nan Zhiqiao and asked mysteriously.

Nan Zhiqiao shook his head with a smile, "It's not what you think, everything is above board, the genetic modification of this animal is allowed by law, and I can apply for project funds, I haven't published relevant papers, because I have participated in relatively few researches, and for some reasons, I quit halfway." ”