Ye Zhenzhen asked: "What kinds of pathogens have been checked?"

"Viruses were tested for sweat and ebola viruses, and bacteria were tested for hemorrhagic Escherichia coli. ”

After Section Chief Zhang finished speaking simply, he also felt that there were some fewer pathogens to investigate, so he took the initiative to say to Ye Zhenzhen: "Teacher Ye, there are few testing reagents in our district's disease control reserves, can the national CDC help......

Before Section Chief Zhang finished speaking, Ye Zhenzhen agreed, "Okay, I will take the patient's specimen back this time, and I will give you feedback in time after the results are available." ”

After collecting the specimens, I also asked the three patients about the situation before the onset of the disease, as well as the condition of the three people who had died.

On the way back to the CDC in China, the more Ye Zhenzhen thought about it, the more strange she felt, the patients sent by Baiyun Ranch this time were all staff of the ranch, and they were all men, which should be related to the environment they were exposed to, but what caused the disease?

After returning to the central laboratory, Ye Zhenzhen put the specimens she brought back from the hospital into the P2 laboratory.

Before doing the experiment, Ye Zhenzhen first called Nan Zhiqiao, mainly to report the situation just now, and also wanted to know Nan Zhiqiao's opinion on the testing of the patient's specimen, after all, there are too many types of pathogens that can cause bleeding symptoms, and a range should be roughly determined before testing.

On the phone, listening to Ye Zhenzhen's description of the symptoms of the patients in Baiyun Ranch, I thought of the experience of chasing wild boars in the woods of the ranch that day, could these symptoms be related to wild boars?

Nan Zhiqiao asked Ye Zhenzhen to send the picture of the patient in Baiyun Ranch to his mobile phone, and in the photo, there were several dark red holes in the palms of the three dead cases, as if they had been pricked by needles.

Seeing the patient's photo, Nan Zhiqiao recognized that these people had participated in the hunt for wild boars, plus the pinhole-like wounds on each person's hand, Nan Zhiqiao gave a suggestion: "First stain the patient's specimen with a smear to see if there are any bacteria, and then carry out the next step of testing." ”

After talking about the testing suggestions, Nan Zhiqiao instructed again: "Pay attention to protection during the experiment. ”

According to Nan Zhiqiao's suggestion, Ye Zhenzhen first performed a simple Gram stain, and the results of microscopic observation showed that the purple globular cells were arranged in a chain.

With the staining results, Ye Zhenzhen hurriedly called Nan Zhiqiao, "Senior Nan, it's a gram-positive streptococcus, and the next step is to do biochemical identification and divide the subtypes." ”

In her mind, she made a list of the types of streptococci that can cause disease in humans: Streptococcus pyogenes, Streptococcus viridansis, Streptococcus pneumoniae......

There are also many manifestations of disease caused by streptococcus: purulent inflammation, toxin diseases, hypersensitivity diseases......

Ye Zhenzhen said the streptococcus she thought of, and Nan Zhiqiao added another, "Streptococcus suis." ”

"Streptococcus suis?!" Ye Zhenzhen was very surprised, and asked rhetorically: "Streptococcus suis is a strain carried by pigs, Senior Nan, do you suspect that this collective illness in Baiyun Ranch is related to pigs?"

"Well, swine streptococcal disease is originally a zoonotic disease, and pathogenic bacteria will be infected through contact, and the sick people in Baiyun Ranch should have had contact with wild boars. ”

Ye Zhenzhen: "Yes, they have all chased wild boars, and the rancher also said that at that time, several of them also buried wild boars with their own hands, and several of them also hurt their hands with wild boar hair. ”

"That's right, there is a way of transmission, and then laboratory testing can determine the cause. ”

Ye Zhenzhen seized the time to do biochemical testing and further classification, and sure enough, it was Streptococcus suis.

First, the experimental results were fed back to the district hospital and the district CDC, and the district hospital adjusted the treatment plan according to the laboratory results fed back by Ye Zhenzhen, and treated with antibiotics sensitive to Streptococcus suis, and the patient's condition was quickly stabilized.

The ability to quickly identify the pathogen has added another layer of admiration to the district hospital and the district CDC's detection ability, and Ye Zhenzhen was surprised by Nanzhiqiao's ability to quickly lock down the scope of the pathogen.

Taking advantage of her free time, Ye Zhenzhen called Nan Zhiqiao and asked, "Senior Nan, how did you quickly lock the pathogen to Streptococcus suis?" ”

Nan Zhiqiao said lightly: "The information you gave reminded me, plus the epidemic here in Sichuan, although they are thousands of miles apart, the symptoms of the patients are very similar, which is also what they have in common." ”

Speaking of common ground, Ye Zhenzhen asked in surprise: "The pathogen of the epidemic in Sichuan is also Streptococcus suis?"

"Well, just after laboratory diagnosis, Streptococcus suis was detected in several previous deaths here in Sichuan, and our expert group preliminarily determined that the outbreak was a human-Streptococcus suis infection caused by Streptococcus suis infection. ”

Although Nan Zhiqiao said it simply, but the matter was very tense and thrilling, the local hospital in Yuanyang City continued to receive patients with similar symptoms, before the pathogen was confirmed, the hospital and the general public were very nervous, and there was once news that a "strange disease" hit Yuanyang, and everyone was in danger.

The team quickly identified the causative agent, and the next task was to investigate the disease, control the spread of the epidemic, find the source of infection and the route of transmission, and finally eliminate the epidemic.

Ye Zhenzhen was still very curious about Nan Zhiqiao's ability to quickly lock the scope of the pathogen, and asked: "Senior Nan, in addition to the patient's symptoms, what other auxiliary clues do you have to determine the detection range of the pathogen?"

"In fact, there are many clues, if you only look at the cases in Yuanyang, you can also draw several conclusions: first, the patients were in good health before the onset of the disease, second, the patients were all 30 to 50 years old farmers in the prime of life, third, the patients had a history of direct contact with sick and dead pigs before the onset of the disease, and fourth, the patients all had symptoms such as high fever, subcutaneous bruising, and shock, and some cases showed the characteristics of acute onset, short course of disease, and high mortality rate. Based on these clues, Streptococcus suis infection is most likely. ”

"Oh, it turns out that I found a clue from the information of these patients. Ye Zhenzhen felt that she had learned a little more, and happily asked: "Then is there a connection between the people who are thousands of miles away and the Streptococcus suis infection epidemic?"

"Now that the strain of the patient has been isolated from Yuanyang's side, I am going to send it to the Guojing Microbial Forensic Science Laboratory, and you also send the strain isolated from the patient in Baiyun Ranch to let Li Guoyi do sequence analysis, there should be a discovery." ”

Nan Zhiqiao assigned the task, and it was a task assigned to the newly built Guojing Microbial Forensic Science Laboratory, Ye Zhenzhen was also looking forward to the performance of this new laboratory, and at the same time, she was also a little worried, and asked: "Senior Nan, the laboratory over there in the Guojing Medical Center has just been built, will there be any problems?"

Nan Zhiqiao smiled: "There is always a beginning, and this is its first task." ”

Ye Zhenzhen has been listening to You Kexin nagging about the Guojing Medical Center recently, saying that Liang Chen is working overtime to debug the laboratory's network, and according to the progress, it should not be debugged yet.