(Thank you for the "streamlining line under the sky" and "Maverick Weiwei" for your tips, and thank you for your collection and recommendation.) Today's weekly recommendation is better than 1700 plus. ）

Gilbert Gordon and nearly a hundred researchers were already waiting in the lab with excited faces, and this time they finally achieved something.

Compared with other laboratories under the corona, Corbister 1 has the highest security level, the largest number of personnel, and the most abundant R&D funds, but it has not been able to see results for a long time, which inevitably makes them feel a lot of pressure, and this time they can finally face Li Jie, the young boss, with their heads held high.

"According to your suggestion, Boss, on the one hand, we continue to increase investment in the research and development of gene reagents, and at the same time, we have begun to try to improve and modify the recipient gene through other direct means." Gilbert pointed to the large display in the center of the lab, which showed his picture in time, several spiral-shaped DNA profiles, and more complex data and explanations.

"These are the two ways in which we finally achieved the modification, using the prototype as a model, generating an artificial genome according to the established protocol, and then replacing the DNA at a specific location of the experimental subject through gene editing technology."

Gene editing technology is not the first of its kind in the Corbyster lab, as it has been a very hot frontier technology in recent years, and has been involved in many institutions, including the George Church lab at Harvard University. This is a technique to complete precise modification of the genome, which can complete the site-directed InDel mutation, knock-in, multi-site simultaneous mutation, and small fragment censoring.

Put simply, this technology can be precisely, effectively and relatively simply replaced DNA at specific locations on chromosomes, targeting and modifying the genes of targets and even primates, including humans, of course. For example, it can be expected that a certain piece of gene from an ape, orangutan, or even a fly will be replaced into a human body by this means.

It sounds a bit like a scene from science fiction and movies, but the truth is that the technology is almost a reality, and the only two issues that stand in the way of breaking through are moral constraints and technical limitations. The former is naturally a long-term debate in the human scientific community, public opinion circles and even moral figures, involving ethical and moral considerations, this controversy has never stopped since the beginning of human cloning technology, but this is still not the biggest problem hindering scientific research, after all, there are medical considerations and life-saving effects.

The biggest problem is still the limitation of technology, including how to improve the speed and stability of genome editing to modify the target DNA sequence. After all, gene editing technology cannot be completely equated with the replication and cutting of software and programs on the computer desktop, and unlike other drug treatments or laboratory tools, it is not a "steady-state" chemical process, only the burst of gene editing activity is short enough to modify one or two target genes, and then this activity can be made to disappear and no longer affect the subsequent process of the cell, can be regarded as a completely successful gene editing.

"But it's no longer a problem for us!"

Gilbert Gordon's eyes flashed with excitement and frenzy, directing the researchers to work in front of the computer. "Our artificial genome synthesized based on prototype reagents can make up for these problems, and we only need to precisely control the editing progress to achieve fast, accurate and stable gene editing."

On the huge central screen, the screen constantly switches with his introduction, simulating the entire gene editing process that Li Jie and his team intuitively understand.

"Currently, there are three major technologies for genome editing: cRIsPR/cas9, TaLen, and zFn. Compared to the traditional TaLen and zFn technologies, the cRIsPR/cas9 system is more convenient and efficient, which is also the solution we use. Gilbert Gordon moved so quickly that he didn't look like a middle-aged man at all, and continued to introduce.

"The rejection that occurred before has been resolved?" Li Jie asked, he has always kept a close eye on the research projects in various laboratories, and receives progress reports almost every day, especially Kobist 1. In the initial experiments of this project, the artificial genome was often recognized by the host cell and taken a defensive posture, which is roughly similar to the rejection of the transplanted organ by the patient's body because it does not match perfectly.

"The rejection occurs because the artificial genome is not a methylation product, it must be grown in yeast, and this process does not provide it with the substances it needs to chemically alter, making it vulnerable to restriction enzymes." Gilbert Gordon motioned for the researchers to continue the demonstration. "So we've added a program to the cultivation of artificial genomes – independent methylation. After this procedure, the artificial genome is well protected against the host cell's defenses, protecting them from chemical (restriction enzymes) and preventing them from being poisoned by viruses. It's like using drugs that suppress the immune system to prevent rejection of implanted organs. ”

After simulating the process of gene editing, Gilbert waved excitedly and guided Li Jie and the researchers to the depths of the laboratory.

The crowd walked until they reached the third cage on their left hand, and saw an unremarkable earthy yellow dog lying in the corner of the wall, staring at everyone with faint eyes. Since this dog is lying on its stomach, it is difficult to accurately judge the mentions, but it can be roughly estimated that it is not too small and belongs to a large dog breed, especially the limbs and body are extremely stout.

"This dog is huge, what breed is it?" Itzikil is a dog lover, but he didn't recognize what kind of dog it was in front of him, but he felt familiar. "How does it look weird?"

"It's a Pitbull." The researchers on the side had obviously seen him, and knew that it was Li Jie's right-hand man, so they did not shy away from replying.

"What? Is this a Pitbull? Are you kidding, it can't be?! Itzikil was taken aback. Is it because of genetic modification? ”

Pitbull is the American Bull Terrier, which originated in the 19th century American fierce dog, generally 46-56cm tall, weighing between 15-35kg, its head is slate-shaped, the jaws are strong, the muscles are strong, the bite is strong, the endurance is long-lasting, the perseverance is amazing, is the super fierce dog in the hearts of dog lovers. The American Federal Dog Association describes it as: a great dog breed with a beautiful body shape, well-developed muscles, extremely intelligent mind, tenacious will, amazing endurance, and good hygiene habits, and considers it the world's number one dog breed. A 30-kilogram strong bit may even defeat the Caucasus, which weighs more than twice as much as itself, which shows the combat effectiveness of this dog.

The only pity is that due to the genetics of the dog breed, the size of the Pitbull is not particularly large, and it is generally difficult to exceed 60 pounds (27.2 kilograms), and the earthy yellow Bit in front of him, no matter how you look at it, is more than one meter tall, doesn't it weigh more than sixty or seventy kilograms! Don't say Izikiel has seen it, and I've never even heard of such a big pitbull.