Ashikaga Yoshiaki believed that the Uesugi family had troops and occupied most of the Eastern Province, and his retainers were also brave and good at fighting, and Fujiwara Asadasei, who served as the head of the Uesugi family, was "long-cherished", and if he continued to indulge, it was likely to be detrimental to the shogunate. Subsequently, Ashikaga Yoshiaki did not listen to the advice of Oda Nobunaga, Asai Nagamasa, and other powerful daimyos and shoguns, and after depriving Uesugi Kenshin, Fujiwara Asada, Usami Chomitsu, Kakizaki Keike, Uesugi Keimitsu and others of their positions, as well as the daimyo who was an enemy of the Uesugi family, he still did not intend to stop, and ordered Uesugi Eizun (Uesugi Tomi Chao's second son, Uesugi Kitomi's brother, Uesugi Mirifuki's brother, Uesugi Hachimangu Pure Monk) and Uesugi Sonjun (Uesugi Tomisa's third son, Uesugi Kitomi's brother, Uesugi Kitomi, Tsuruoka Hachimangu Jokokuin monk) , Uesugi Yoshiji (Fukaya Uesugi, the younger brother of Uesugi Ken, Uesugi Kenmori Sano) and others came out of Horikoshi Gongfang, and replaced Kenshin with Uesugi Rongzun as the Kanto governor, Uesugi Sonjun was appointed as the Kanto governor and Uesugi Yoshiji was appointed as the lower chief guardian. It is hoped that the forces that are enemies of the Uesugi family, such as the Horikoshi family, the Fukaya Uesugi family, the Takeda family, and the Odawara Ise family, will be able to curb the development of the Uesugi family and eat the Uesugi family as much as possible.

However, Ashikaga Yoshiaki did not expect that a crisis was rapidly approaching him.

At the beginning of the fourth year of Eiroku, when the general Ashikaga Yoshiaki was in Kamiraku, Oda Nobunaga quickly defeated the forces of the Miyoshi and Sanfang, and swept their territories in Yamashiro Province, Kawachi Province, Setzu Province and other places, forcing the Miyoshi Sanren and Miyoshi Yasunaga and others to flee back to Awa Province and Sanki Province in defeat. Therefore, the Miyoshi Sanren and the Miyoshi forces such as Yasunaga Miyoshi and Shinohara, taking advantage of the fact that Oda Nobunaga, Asai Nagamasa and others left Kyoto and returned to their respective cities, gathered the former Mino guardian Saito Ryuko and his former retainer Nagai Michitoshi (former Saito Yoshiryu's side approach, former Saito Yoshiryu's elder, Hara Mino Guardian, Saito Ryuko's posterior, Saito Michi's three adopted sons, and former Kanayama Castle Lord), Nagai Michikatsu (Nagai Michitoshi's eldest son), Fukasawa Seisa (former Saito Yoshiryu's banner), Fukumitsu Yorikuni (former Saito Yoshiryuki Honko) and others attacked Ashikaga Yoshiaki, who was temporarily residing in Honkuji Temple (Kuniji Temple, Nichiren sect Daihonzan, mountain name "Daikoyama Mountain"), with Yakushiji Sadahunu (lord of Izumi Kuniren Province and former Miyoshi family Settsu people) as the spearhead.

After the Miyoshi army landed from Izumi Province, on February 7, they first attacked Iyahara Castle, which had fallen to the side of the Oda and Ashikaga families, and the castle commanders, such as Hakube Jibei and Teramachi Shogun, were killed in the battle, and more than 200 castle soldiers were killed. After the ensuing victory, the Miyoshi army attacked overnight, approaching Honkuji Temple, where Ashikaga Yoshiaki lived, on the night of February 9. At that time, there were a total of Akechi Mitsuhide, Hosokawa Fujiken, Mibuchi Fujiei, Oda Sakon, Nomura Echichu Mamoru and Tsuda Saoma Majo, as well as well-known warriors from Wakasa Province, such as Genuchi Yama and Yashichi Uno. Ashikaga Yoshiaki learned that the three of Miyoshi were leading an army to attack, so he had to order Hosokawa Fujiken and Mibuchi Fujiei to guard the main gate of Honkuji Temple, and Nomura Echichu to guard the Dumen Gate. Takatsuki Castle Commander Akaza Eikane, Moriya Gohachiro, Sakai and Uemon also took the lead in coming to the rescue day and night, entering the main temple to assist in the defense, and Ashikaga Yoshiaki quickly sent an envoy to ask for help from the powerful generals of the four directions.

After the Miyoshi Army besieged the temple, Miyoshi Nagayi and Miyoshi Masaysu led more than 3,000 troops to attack the north of the temple, Iwanari Tomotsu led more than 1,500 troops to attack the west side of the temple, and Miyoshi Yasucho led more than 3,000 troops to attack the east side. At the same time, the various units of the Miyoshi Army also set fire to the periphery of the temple, and the defenders in the temple also opened the door for a time to engage in a confrontation with the Miyoshi Nagaitsu Division. Yakushiji Sadadaharu, as the vanguard of the Miyoshi side, attacked the defenders of the temple several times, and even Yama Prefecture Genuchi and Uno Yashichi fought to the exhaustion, and died under the spears of the Miyoshi army. The Miyoshi army almost invaded the temple, although the situation was quite chaotic, the defenders of the temple also used bows and arrows or iron cannons, killed more than 30 enemy soldiers in one round, the battle was very fierce, the sound of iron cannons almost shook most of Kyoto, but after several repeated battles, neither side saw victory or defeat. Miyoshi had no choice but to rebel against the lord of Takatsuki Castle, Irie Harukei, and sent Miyoshi Masakatsu and Miyoshi Nagakatsu to lead an army into Takatsuki Castle in an attempt to contain Ikeda Katsumasa and Itami Kinko, and other powerful Gyeonggi generals, so that they could not successfully lead their troops to rescue Honkuji Temple.

On 8 February, after learning that the Miyoshi Army was attacking the temple, Miyoshi Yoshitsugu immediately led more than 3,000 troops to the rescue. At the same time, the lords of Hosokawa Fujitaka, Araki Murashige, Katsumasa Ikeda, and Itami Kinko of the Oda and Ashikaga forces quickly gathered, and although Ikeda Katsumasa and Itami Kinko were hindered, they took another path to merge with the forces of Miyoshi Yoshitsugu and others, and Miyoshi Yoshitsugu and others tried to force the Miyoshi Army main camp on the Katsura River from the rear, and Miyoshi Yoshitsugu and others were divided into three formations, Ikeda Katsumasa and Itami Kinko attacked from the west, Miyoshi Yoshitsugu attacked from the south, and the Honkuji defenders attacked from the north. However, the Miyoshi trio also divided their forces in two, Miyoshi Nagaitsu and Miyoshi Masayasu continued to attack Honkuji Temple, and Miyoshi Yasunaga and Iwanari Tomotsu led their troops to Katsura River to engage Miyoshi Yoshitsugu and others, and successfully repelled Miyoshi Yoshitsugu and Ikeda Katsumasa, Ikeda Katsumasa had no choice but to retreat to Ikeda Castle, and Miyoshi Yoshitsugu was forced to turn to Mt. Rakusai to collect the remnants, and there were even rumors that Miyoshi Yoshitsugu was begged to die in the war.

Although Miyoshi Yoshitsugu, Ikeda Katsumasa and other troops were repelled by the Miyoshi trio, Hosokawa Fujitakabe continued to fight, and Itami Jinxing went straight to the Miyoshi army headquarters where Miyoshi Nagayi and Miyoshi Mayasu were located. After the fierce battle between the two sides, the Itami army killed more than 100 people, but Itami Jinxing still fought unremittingly, the defenders of the main temple under the leadership of Hosokawa Fujiken, Mibuchi Fujiei and others took the initiative to open the door to rescue Itami Qinxing, the two sides should be combined to successfully defeat the main formation of the Miyoshi army, Miyoshi Nagayi and Miyoshi Masaysu were forced to retreat to Fushimi and Toba area, and the Miyoshi army was killed by more than 2,700 people. After the victory over Hosokawa Fujitaka, Itami Shinko, Ikeda Katsumasa, and Arakimurashige converged into Hongi-ji Temple.

Although the Miyoshi army was only temporarily defeated, Miyoshi Nagaitsu and others were worried that the war would be protracted and time-consuming, and that powerful daimyo such as Oda Nobunaga and Asai Nagamasa would soon lead troops to help, so the generals were distributed in important places such as Yodo Castle, Hachiman, and Fushimi to take precautions. Itami was wounded after the war and resided in Katyuji Castle, while Hosokawa Fujiken and others returned to Honkuji Temple to continue their defense, and Miyoshi Yoshitsugi, Hosokawa Fujitaka, and Ikeda Katsumasa also entered Katryuji Castle on February 9.

Oda Nobunaga, who received an urgent report that the temple had been attacked, had to lead his army in the snow on February 8 in order to rescue Ashikaga Yoshiaki, shortening the trip that would have taken three days to two days, and the army under his command even froze to death, and finally Nobunaga led more than a dozen horsemen into Kyoto first, and then the Oda army reached a total of more than 6,000, because Oda Nobunaga had originally prepared to attack Minami Ise with the intention of destroying all the wealthy clans loyal to the Kuniji Kitabatake clan, so a certain number of troops were gathered near Gifu Castle. When Oda Nobunaga learned of the Miyoshi Army's attack on Honkuji Temple, he had to temporarily suspend his invasion of Minami Ise and go to Gyeonggi to rescue Ashikaga Yoshiaki.

After Oda Nobunaga entered Kyoto, in addition to rewarding the meritorious people Ikeda Katsumasa, Itami Kinko and others, on the 15th, he also informed the Mori family, Otomo family and other distant daimyo of the battle situation, and at the same time, Oda Nobunaga felt that the garrison of the temple was insufficient, and at the request of Ashikaga Yoshiaki, he began to build Nijo Castle as an imperial residence for him to live in.

On the other hand, the Okano (Tanaka, Tsubibuoka) family of the former Sagami country went against the trend, and Okano Hideaki led more than 100 people from the Lang clan to join his old master, the Odawara Ise family.

Why did Hideaki Okano go to Ashigara-gun to join the old lord Odawara Ise family when most of the wealthy families of Sagami were falling to the Uesugi family? The reason is that the previous Okano clan's drip wrench Okae Xue (Rongcheng, Tanaka Rongsei, Okano Rongsei) came out of the Hojo family to serve as the head of the Houhojo family, which led to the confiscation of all the territories of the Okano clan in Sagami, and the territory of Izu was also encroached upon by the Takeda family, so for the sake of livelihood and revenge, Hideaki Okano had to take the clan Lang Party out of the Odawara Ise family, which is equivalent to a disguised appearance of the Takeda family.

Not only that, Ishinomaki Tenyongin (Hojo clan Yasushi diplomatic monk/envoy monk, Ishinomaki Yasumune Sano), Ueno Sunzaemon (Izu acting official), Matsuda Yasumitsu (Matsuda Kenhide's younger brother, three family elders), Yamato Haruhin (assessor), Ishigami Yajiro (Imperial Family), Oto Shinbei (Ashigaru), Kato Shiro Saemon (Ashigaru), Tamai Obito Saemon (Powerful Official, Ashigaru), Otani Hikojiro (Ashigaru), Kagamino Echizen Mamoru (other people) and other former Go-Hojo family retainers who were hiding in various parts of the Kanto region, as well as ronin from Kanto countries who did not want to go out of the Uesugi family, also went to the Ise clan Takashi and Horikoshi Gongkata in a vain attempt to regain control of the Sagami country and make the Go-Hojo family revived.

It was not that these people took the initiative to defect to the Horikoshi Kokata and the Odawara Ise family, but that the policies pursued after the Asadaki regained control of most of the eight countries in the Kanto region were completely different from those implemented during the period of the Later Hojo family's occupation.

Previously, the Hojo family ensured that the shrines and temples in the occupied areas (Taihei-ji Temple, Matsuoka Dongkei-ji Temple, and Hakone Gonen) had a part of the Zhixing, and gave some of them the privilege of guarding them. Since the Imperial Decision recaptured the old domains such as Musashi and Sagami, it directly abolished the guardian and confiscated most of the monasteries and shrines, which harmed the interests of many national lords and local samurai. The wealthy clans and samurai who had been in the Hojo family and held important positions before they came to the house were directly ordered by the court to confiscate all their family money and territories, and those who were so guilty were exiled to remote areas such as Nanjioku and Sado.

The Kamakura Gozan (five temples located in the Kanto region and on par with the Kyoto Gozan in the Kanto region, Kenchoji, Enkaku-ji, Sutomi-ji, Jochi-ji and Jomyo-ji) were originally under the jurisdiction of the Kamakura Kokata (Kanto Kokata) and the control of the Kamakura Gozan was taken over by the Uesugi family after the Asada's conquest of most of Sagami Province, and the Kanto Kokata Imagawa clan, which was supported by the Asada, only owned the Chiyuki land of 1,000 koku in the Kamakura area.

Ishinomaki Tenyongin and other former retainers of the Hojo family came out to the Ise clan, which greatly improved the current situation of the Odawara Ise family in repeated defeats and low morale, and Iseji Takashi instantly had the confidence to fight with the Uesugi family.