Whenever a new country is formed, Downing will always give the new country a little new atmosphere, what sugar-coated cannonballs, strange buildings, and so on. This time, the establishment of the Chinese Federation coincided with the outbreak of horse flu in North America, which began in Toronto and spread to New England, becoming an international hot topic.

The western part of the United States, which is under the banner of the Windsor Empire, immediately launched strict quarantine procedures, taking the opportunity to issue a "special number" of "Nature" to disclose a working method of the quarantine agency under the Windsor Empire. That is, genetic testing of horses, donkeys, mules and other disease receptors entering and leaving the country.

First of all, the genetic characteristics of the equine influenza virus should be found out, and then compared with the quarantine results. Among them, the most technical content is gene sequencing technology. It is also the reason why "Nature" has become a hit.

This time, in order to boost Beijing's popularity, he used the name of the newly established Pasteur Institute of Peking University.

The sequencing method published in Nature is the deoxyribonucleic acid chain polymerization abort method, which uses a nucleotide with one less oxygen than normal DNA to mix into the polymerization reaction, so that a large number of chains stop replication by adding a dideoxynucleotide at a random position, so that a large number of chains of different lengths are obtained.

Theoretically, a million base pairs need at least 1 million short chains of length, and each of these 1 million short chains can be labeled with dideoxynucleotides, and the end of these 1 million short chains can be measured to obtain the base pair sequence of the entire long chain.

So the question is, how do you know which of the four dideoxynucleotides is added to the end? The magic of Nature is that it solves this problem by dividing the DNA into four parts, each of which only adds one dideoxynucleotide, so that it is certain which dideoxynucleotide a certain part of the DNA terminates, and the only difference in each piece of DNA after being labeled is the length of the strand.

Then measure the length of the chain to get the position of the nucleotide, then you can use the method of electrophoresis, let the short chain race in the electric field, the shorter the chain by the field (gelatin) resistance is smaller, the farther it runs, so that the length of all the short chains, a length corresponds to a position, then measure all the positions of a certain nucleotide, measure four times, all the information is obtained.

This is the end of the article in Nature, in fact, the ability to press the bottom of the box will not be announced, the method used by the Downing team is not to stupidly record the results of electrophoresis one by one, but to stain the DNA strand with fluorescence, and only need to analyze the imaging on the sensor after electrophoresis.

This is a very classic PCR, when the real large-scale sequencing, electrophoresis will become a speed bottleneck, Downing's real killer feature is that there is no electrophoresis at all, a short chain race will take more than 20 minutes, and you can't afford to hurt. So high-throughput detection technology is about to be born.

The core principle of high throughput is still inspired by PCR, divided into four groups, each time four nucleotides are added, but only one of them is an enzyme cascade, one chemical catalyzes the other, a total of four, combined into a reaction chain, the final goal is to make DNA replication grow while emitting light, in this chain generation timeline will appear one peak after another, clearly showing the position of a certain nucleotide on the chain.

This is named after one of the core energy transmitters, called pyrosequencing. Its speed is miraculous, capable of analyzing millions of samples in a single reaction.

DNA molecules are too large and are often cut into very large samples before they are analyzed. In medicine and quarantine, sometimes the DNA molecules of various organisms are stacked together, or they can be mixed together and measured at the same time.

Human DNA has about 3 billion base pairs, and if only the PCR method is used, it will take decades to measure, but the time taken by the pyrophosphate method can be measured in "days", and the huge number generated has become a difficult point of analysis. This miracle technique Tang Ning will not be made public for the time being, and the PCR paper is already a sensation.

Peking University became famous in the first battle and became a leader in the field of biology, and there were so many Chinese students who consulted and actually applied for the examination that they crossed the river, and the aborigines of Beijing wondered, why did so many four-eyed foreign devils come all at once?

The Windsor Continental Empire has set up a gene sequencing center for each continent, with Asia in Beijing, Europe in Luxembourg, North America in Seattle, (Seattle again, Jet has been placed here, and to add to it, because it is necessary to disperse San Francisco, whose population has expanded very much), South America is in Rio de Janeiro, and there is also one in Africa, and Canberra on the edge of Lake Victoria, the largest lake in Africa.

This is the first batch, in fact, India, Israel, the Soviet Union and other large countries will also have sequencing centers later. In order to stop the spread of equine flu, the United States and Canada, as Entente powers, had to condescend under the pressure of herders who used horses for animal husbandry and thus suffered heavy losses, and outsourced customs and quarantine departments to the Windsor tech empire, and New York customs flew to Seattle every day just to more accurately test the virus genes for horses, mules and other domestic animals.

Biologists in the Entente countries are actually still wondering, if the detection efficiency of PCR is followed, the Seattle Gene Sequencing Center cannot complete the sequencing of such a large number of samples on time every day, isn't it fooling us?

I can't help it, the scientists of the Entente countries have preliminarily reproduced the work of PCR and think that it is reliable. However, they can only sequence in small batches in the real room, and they do not have the ability to set up their own testing center for the time being.

Once again, the United States, Britain, the Netherlands, Canada and other countries have outsourced their quarantine departments, Japan hesitated again and again, and also joined the ranks, while France, Prussia, the Qing Dynasty, and Russia still held their positions, replacing reason with politics, and it was a big deal to never let foreign horses enter! In the Qing Dynasty, even beef and pork were banned, and a single qiē livestock was forbidden.

If you look at the virtue of the Qing Dynasty, you will know the irrationality of the civil subject, so Tang Ning was very emotional, and decided that the Chinese Federation would reduce the interference of public opinion on the administration and implement the autonomous program of the academic committee to control the leadership.

Chinese attach great importance to the ranking of departments, Tangning also has to follow the local customs, the importance of the academic committees in line, the first is the environmental protection and sustainable development committee, the chairman is naturally Chinese, but because China has not paid attention to the environment, this committee has many foreign members.

Surprisingly, the second place is the Chinese Studies Committee, which is full of disdain for Chinese culture. It can be seen that what he said about the scientific concept of development with good intentions for Chinese culture is not just talking, and so many interesting things in Chinese culture naturally need to be well protected, such as the literati tradition of qin, chess, calligraphy and painting, especially painting, according to Tang Ning's view, it is much stronger than Japan's ukiyo-e or something.

However, these people who can be included in the Chinese Studies Committee have a certain scientific foundation, and if you want to use the medical skills and feng shui techniques handed down from ancient times, it will be difficult.

One of the things that the Chinese Studies Committee has done to protect folk customs is to revive the tradition of firing artillery during the Chinese New Year, of course, it has compromised with the Environmental Protection Committee to encourage the production of electronic artillery instead of physical artillery such as black powder and other guns. This electronic artillery battle is remarkable, with GPS, it is only allowed to sound in certain specified blocks, and there is a time limit, it is impossible to give you three pounds in the middle of the night to give you a cannon battle, even on Chinese New Year's Eve, after one o'clock in the morning, you will not be allowed to move.

Ranked third: Committee of the Chinese Industry Association. Responsible for formulating the rules of all walks of life, there are now a lot of various guilds in China are very sound, and the policies are mainly put forward by them, and finally the committee acts as a referee to coordinate the interests of the guilds.

The importance of this sector lies in the fact that the economic development depends entirely on them, and the core of economic modernization is the modernization of vocational education.

This industry committee has also brought in a large number of foreign members, especially in Germany, whose vocational education is the world's leading member.

Fourth: the Judicial Council. The old rules brought in British and American foreign commissioners who were quite talented in the judiciary to help China legalize it.

With the reorganization of the country, the great Independent Commission Against Corruption can be resurrected with full blood, and the same implementation of the world's attention is "bribers are a vulnerable group, not punished by law, reports can recover bribes, and the relevant departments have the responsibility to protect whistleblowers that cannot be ignored." Recipients of bribes are subject to heavy penalties, up to a life slave to serve their sentence in Brazil. ”

This is very similar to that of Guo Songtao's administration, which greatly increases the possibility of long-standing suspicion from the outside world: the previous Qing Dynasty was probably reformed under the impetus of Windsor, otherwise it would not have been so radical and radical.

Sixth: Commission on Human Rights. The point is that the Commission is responsible for providing basic subsistence benefits to anyone in the United States of China, whether a citizen, a tourist, or an illegal immigrant, who has the power to apply for identity verification and receive free food.

The city's central kitchen sells food in the form of vending machines at free food distribution centers, and those who are in a state of proletarianism can get a free food with a wristband containing their identity information.

And citizens, there is the right to apply for free housing, this of course depends on the productivity of the government, like ** low-rent housing of 100 square meters who does not like, but now the country's population base is large, it is unlikely to have that kind of welfare.

The exception is that the people who have passed the primary education do have the right to housing, and the state will give priority to their right to live, and all the initial productive forces will be used to build free apartments for the "literate people" until they find a job.

After finding a job, you can still live in a free apartment that is not wrong, but you have to start paying a certain amount of rent so that the state can further expand the welfare population. The Chinese Federation aims to build housing benefits for all citizens in 10 years.

The house is not good, the clothes are much simpler, the Human Rights Commission has a unique benefit of sending a new dress to the people, or ask a famous Italian designer to design, simple and good-looking, but also save materials, if you are not "your own person", at first glance on the road, I thought it was a fashion brand, why in all the shopping malls have not seen it to sell?

In order to maintain the claimant's **, the style of these free new clothes will not be advertised, and the style will be changed every once in a while, and it will definitely be changed every season.

There are basic guarantees for food, clothing and housing, and even travel. Buses from shelter to free food distributions are free for applicants, and long-distance travel in places with a large demand for labour is also free, encouraging unemployed vagrants to go there.

Seventh Committee: Committee on Intellectual Property. We will protect intangible patents, copyrights, and other things that do not exist in traditional culture.

These are all rather bizarre committees, all of which occupy the top rankings, while taxation, finance, police, transportation, postal services, and national defense are all relegated to the back, greatly weakening the position of these traditional "powerful" departments.

A great feature of these committees is that they employ a large number of foreign members, which have become the highlight of the Great Qing State's sarcasm, sarcasm, ridicule, and belittlement. "Look, I'm right, right? The country will not be a country, the foreigners have ruled my Qing Dynasty, and in the future everything will have to listen to the foreigners, sue you, those Chinese are all decorations, do you think, what good fruits will the puppet regime established by a foreign devil as a big boss give you to eat? ”