As soon as Shiho Miyano said this...

It's like the homeroom teacher who appeared outside the window for evening self-study, and the air suddenly froze.

In the dead air, Mr. Yuda's face became paler and his breathing suddenly became heavier.

At first glance, it is impossible to tell whether his symptoms have become severe because of mild tetrodotoxin poisoning, or if he has been suddenly told something on his mind, so that he is so shocked.

"Call the police?"

At this time, Lin Xinyi was still busy helping the poisoned lady with heart compressions and artificial respiration, and he couldn't open his mouth for a while.

However, Mao Lilan reacted with some shock:

"Miss Miyano, what do you mean..."

"This is not ordinary accidental food poisoning, but someone is deliberately poisoning?"

"Quite possibly."

Shiho Miyano put his hands in his pockets and said nonchalantly:

"Fugu dishes are all non-toxic, and even if they are not cleaned occasionally, with the accidental residual trace toxins, it is rare for such rapid acute tetrodotoxin poisoning to occur."

"This Mrs. Yuda should have been poisoned."

"And the one who poisoned him..."

"Probably the only one in this box, Mr. Yuda."

This time, it was no longer a meaningful hint, but an undisguised suspicion.

Yuda's face suddenly turned extremely pale.

He forcibly held on to his body paralyzed and out of control by tetrodotoxin, and said indignantly:

"Hey, what are you talking nonsense?"

"How could I possibly poison my wife?!"

"Besides... I、I've been poisoned myself, so it's obvious that there's something wrong with the fugu food served by the store! ”

"This—" Maolilan frowned tightly, a little unsure.

She thought about it carefully, and finally felt that the arguments given by Shiho Miyano were a little unreliable:

"It's rare to have this kind of acute poisoning when eating puffer fish dishes, so it's not that there must be none, right?"

"It's not impossible if the restaurant made a mistake in handling the fugu and left a lot of toxins in the dishes."

"No... You couldn't be more wrong. ”

"Because this Mrs. Yuda's poison is so fast and the symptoms are so serious, it is no longer an ordinary acute poisoning."

Shiho Miyano sighed softly and continued:

"Probably you know tetrodotoxin is very deadly."

"But contrary to what you think, even if you eat a lethal amount of tetrodotoxin, people will not get sick and die so quickly."

"Because eating puffer fish meat is equivalent to oral administration from the stomach and intestines."

"As for gastrointestinal administration, because it involves the absorption rate of the drug in the gastrointestinal tract, the drug needs to eliminate the interference of food, and it has to pass through the cells of the intestinal epithelium, be processed by the liver, and finally enter the blood circulation."

"Therefore, the bioavailability of oral drugs, that is, the speed and extent at which the drug is absorbed into the body's circulation, tends to be relatively low."

"Speaking of which..."

Miyano Shiho paused slightly, making a reminiscence:

"When I was 10 years old, I happened to publish a paper on "Pharmacological Analysis and Pharmacokinetic Studies of Tetrodotoxin."

Yuda and Moriran: "??? ”

Lin Xinyi: "......"

Everyone's eyes were a little strange, but this genius science girl ignored this ordinary question, and continued to herself:

"At that time, when I was doing animal experiments on mice, I found that the LD50 administered by gavage was 12 times that of intraperitoneal injection, which indicates that the bioavailability of TTX (tetrodotoxin) is greatly reduced when administered by gastrointestinal administration."

"That's right, LD50, half lethal dose, indicates the minimum amount of toxin required to kill half of an animal of a certain weight or age through a specified route of infection within a specified period of time."

In order to prevent Mao Lilan from not understanding, Miyano Shiho also specially popularized the professional terms he used.

But Maorilan was still confused.

Yuda was covered in a cold sweat when he heard it—although he couldn't understand it, he seemed to be very powerful.

"Anyway, the conclusion is..."

"Oral administration of tetrodotoxin greatly reduces the speed and extent of the toxin's spread in the human body."

"In other words, compared with direct intravenous injection, the time of poison and death of oral administration will be greatly prolonged."

Shiho Miyano finally said something that was slightly understandable:

"According to studies, patients who take tetrodotoxin orally have an incubation period and the duration of the poison is generally within minutes."

"Death can occur within 10 minutes at the earliest, and 4-6 hours at the latest."

"That is to say... The absorption and distribution of the drug in the human body itself takes time, from oral administration of tetrodotoxin to onset of illness and death, and in the current real-world cases, it takes as little as 20 minutes. ”

"And Mr. Yuda..."

She turned her head and looked coldly at the pale-faced Yuda:

"You just said that this pot of puffer fish hot pot will only be served on the table for about 10 minutes, right?"

"How could a patient become so ill in such a short period of time to the point of dying?"

"I..." Yuda was speechless for a while, and his expression was extremely exciting:

He didn't expect that such a simple serving time could be seen by the girl in front of him with such a big flaw.

How so... Just after poisoning, you can go out and meet a scientist who has studied this toxin?

What kind of luck is this!

If I knew this was the case, I would have started later.

Yuda suddenly fell into apprehension and uneasiness.

And at this time, I only heard Shiho Miyano continue:

"Why did Mrs. Yuda become ill to the point of dying in 10 minutes, maybe less than 10 minutes?"

"There's no evidence for that, but if I had to guess..."

"You must have used a syringe or something like that, and injected tetrodotoxin directly into your wife's vein."

"Because, when tetrodotoxin is taken orally, the first thing tetrodotoxin stimulates is the first contact with the gastrointestinal tract."

"In this way, as soon as the poison is attacked, there should be symptoms of nausea and vomiting like Yuda-sama, even if there is a slight paralysis of the fingers and limbs."

"But Mrs. Yuda has not yet developed symptoms of nausea and vomiting, and she has already developed extremely severe systemic neuromuscular paralysis."

"This suggests that it is very likely that she was not injected with the toxin orally, but by the toxin."

"The toxins go directly into the circulatory system, which is why they cause muscle paralysis of the whole body first, but do not cause nausea and vomiting."

By analyzing Mrs. Yuda's condition, Shiho Miyano roughly deduced how Mr. Yuda was poisoned:

"In doing so, the onset is faster and the fatality rate is higher, and it can almost paralyze the muscles on the spot and incapacitate them, and then kill them in just a few minutes."

"With this fast-acting method of administration, you can safely pretend to be a victim of food poisoning and rush to call for help and an ambulance for your wife."

"Because you know very well—"

"If you take it orally, there is a good chance of being rescued."

"But the wife who was injected with tetrodotoxin by you intravenously won't last long before she will die of poison, and she can't wait for the ambulance to come."

"With this method, you can ensure that your wife will not be rescued after being poisoned, and you can ensure that your wife-killing plot can succeed at one time!"

Shiho Miyano finished talking about this dark and heavy speculation in a cold tone.

And Yuda gritted his teeth tightly, he couldn't refute the "malicious speculation" of Miyano Shiho, and he could only roar inwardly after holding it for a long time:

"What's the joke... I said that I poisoned my wife by injection, but I was poisoned myself! ”

"That puffer fish hot pot must be poisonous, otherwise you can test it."

Yuda justified himself hysterically.

And Miyano Shiho's face still has no waves:

"There is no contradiction between the poison in the puffer fish hot pot and the injection of tetrodotoxin into your wife."

"After all, the poison in the puffer fish hot pot should also be your own."

"If you don't poison the food, how can you disguise this wife-killing plot as accidental food poisoning?"

"As for yourself being poisoned... That's also what you want to make the illusion of 'food poisoning' more credible, so you grasp the dosage and control yourself to be mildly poisoned. ”

Yuda was silent for a while.

He gritted his teeth and refused to admit it:

"Nonsense! It's all just speculation on your part, little girl. ”

"I learned a little bit of nonsense and ran out and talked nonsense..."

"Say I killed my wife, do you have proof?!"

"Evidence?"

As if thinking of something funny, the corners of Miyano Shiho's mouth rose to a playful arc:

"Have you forgotten that your wife is still alive?"

"I—" Yuda choked on suddenly.

And Miyano Shiho continued to taunt him:

"Your wife-killing plot is indeed vicious."

"But it's a pity, you didn't expect that as soon as you went out, you ran into me and Lin Xinyi."

"The two of us work together to save even the 'dead' who no longer perceives signs of life."

"Your wife doesn't have to die now."

"Am I speculating, or are you maliciously quibbling, and when your wife wakes up, she will know everything."