Chapter 132: Shiho Miyano Student Figure

Lin Xinyi didn't pay too much attention to the deadline given by Gin, because he couldn't tell the date at all.

But Shiho Miyano kept this deadline in his mind deeply.

Because she knew that it was not only the deadline for Lin Xinyi to resign and leave, but also the deadline for her and Lin Xinyi to be separated forever.

After all, Gin hated Lin Xinyi for associating with her.

He issued such an order, not only to ask Lin Xinyi to get rid of the connection with the laboratory in a low-key manner and devote himself to being an undercover agent for the police, but also to use this method to cut off the connection between Lin Xinyi and Miyano Shiho on a physical level.

It's like a parent who discovers that their child is in early love at school...

Gin directly asked Lin Xinyi to "transfer schools" to achieve the purpose of forcibly demolishing the couple.

Shiho Miyano knows a lot about these things.

Therefore, she already knew in her heart:

She and Lin Xinyi are like mice in the control group and the experimental group, and they can never be together.

And if an experiment is doomed to fail, there is no need to do it at all.

Therefore, even if Lin Xinyi has been showing favor and confessing to her these days, even if she has been faintly touched in her heart, Miyano Shiho has never let go-

Lin Xinyi is not sensible, she has to understand.

After the last month passed, and after the deadline came, Shiho Miyano chose to let it go.

She regarded Lin Xinyi as a good memory of the past and buried it forever in her heart.

But at this time...

Lin Xinyi appeared in front of her calmly as usual, as usual.

"I didn't expect you to even listen to Gin's orders."

Shiho Miyano gently put down the sandwich in his hand and elegantly wiped his lips with a tissue, before trying to put on her usual cold expression and looking at Lin Shinichi with a deep gaze.

"You used to be like a machine that only obeyed orders."

"I've never seen you before, what would you do for..."

"It's crazy like this."

She said what seemed to be mockery, but there was a hint of warmth in her tone that was difficult to hide.

"I..." Lin Xinyi's expression was complicated:

He's kind of miserable by that wonderful timeline...

In the future, if you encounter this kind of thing that needs to remember your days, you still have to pay attention to asking the people around you clearly.

Also, that bastard Yamada... When you stop people, you should be firm and make your words clear!

How can I release water just because I'm a big brother?

Alas... It's just that if you disobey the order once, there shouldn't be any consequences, right?

Lin Xinyi's heart was entangled, and that trace of worry couldn't even be hidden on his face.

"Sure enough, he's also uneasy..."

"But he came anyway to see me... Oh, what a fool. ”

The corners of Shiho's mouth rose slightly, and the blue pupils flickered with a glittering light.

It's like a cold cat who is carefully cared for by a shovel officer, and is in a happy mood but doesn't want to show it too much.

"That's... Miyano, I'm actually here to find you today. ”

"I know." Miss Miyano didn't even realize she was laughing.

"Are you free today?"

"Free." Miss Miyano was already planning to skip work after eating.

Lin Xinyi dared to come to her with the threat of being punished by gin, didn't she dare to delay a day's work for this?

"Well, listen to me..."

"Hmm... You said. Miss Miyano was a little apprehensive.

She knew that Lin Xinyi should be about to confess again.

And her pounding heart was telling her that at this moment, in the face of Lin Xinyi's irrational crazy actions, her reason would probably go offline...

Perhaps, she would be tempted to say yes.

Miyano Shiho thought so nervously, uneasily and expectantly, only to hear Lin Shinichi after sorting out his thoughts, he said seriously:

"Miyano, I want to ask you to be my ..."

"When yours what?" Miss Miyano pursed her lips nervously.

"Teacher." Lin Xinyi replied.

Shiho Miyano: "??? ”

But Lin Xinyi didn't notice her suddenly cold face at all, and just said to himself:

"Recently, there was a case where an old blood stain that had been sitting for 12 years needed to be examined."

"However, the identification technology of the scientific research is backward, and there is no way to extract effective DNA samples from old paper objects."

"I don't know much about DNA extraction and testing techniques myself, but you're a genius biologist."

"I want you to be my teacher and help me studyβ€”"

"How can we improve the existing DNA extraction methods to extract DNA from old bloodstains?"

Shiho Miyano: "......"

The atmosphere is all up...

Is it time to talk about scientific research?

Speaking of... Last time too.

Last time, he accompanied himself back to the company under the pretext of eating, and made the atmosphere so warm and touching.

At the time, I thought he was going to take the opportunity to say something, but I didn't expect it...

The guy actually pulled her and asked all the way about the toxicology and mechanism of action of tetrodotoxin.

Why do you have a lower EQ than I do? Lin Xinyi.

Shiho Miyano has been out of place with his peers since he was a child because of his high IQ, and he has always gone his own way, not caring about the world at all.

But now she finally knows how tiring it is to deal with someone who doesn't understand the world.

"Miyano, Miyano?"

Lin Xinyi thought that Shiho Miyano was in a daze.

But Shiho Miyano sighed helplessly:

How could anyone run to the risk of disobeying Gin's orders just to come and ask her a scientific question...

It's too much... Wait a minute...

Shiho Miyano, who was about to complain about Lin Shinichi in his heart, suddenly realized this man's innocence:

"I see... He wanted to take advantage of this opportunity to ask someone to help research DNA extraction technology, so he came here to see me. ”

"Gin can also have a high-sounding excuse to be held accountable afterwards."

"But that's stupid..."

"Gin won't believe that he is just here to communicate and work."

"Because I want to fall in love, my IQ has dropped?"

Shiho Miyano ruthlessly complained in his heart about Lin Shinichi's mentally retarded behavior.

But at the same time, she couldn't help but think of the last time she was in the Japanese restaurant, she was like a brainless star-chasing girl...

Well, half a pound and eight taels.

While thinking wildly, Shiho Miyano divided part of his attention and casually picked up Lin Shinichi's words:

"Extract DNA from 12-year-old blood stains on paper objects, right..."

"I haven't studied DNA from blood stains, but I can try."

DNA extraction technology should be used by forensic doctors, and even more so by scholars engaged in biomedicine and genetic engineering.

Therefore, Shiho Miyano really understands better than Lin Shinichi.

As soon as she spoke, she asked the key question:

"Lin, what kind of DNA extraction method is generally used in scientific research and research?"

"Salting-out method." Lin Xinyi replied.

"Salting-out method... It's easy to fail. ”

"If the blood is left out for a long time, the red and white blood cells will definitely be lysed to a certain extent. The formed fragments contain a large number of biological macromolecular impurities such as fats and proteins, and they are distributed in uneven blocks. ”

"If the traditional salting out method is used, after adding the protein precipitate solution, the DNA cannot be completely dissociated from the protein and precipitated together with the protein, which will cause a part of the loss."

"Hmm."

Lin Xin nodded, and added:

"So I went to the Kosou Research Institute and asked them to use the Chelex-100 method for DNA extraction."

Chelex chelates multivalent metal ions, such as magnesium ions. Just as a magnet adsorbs iron, magnesium ions are adsorbed and connected. When magnesium ions are removed from the reaction, the nucleases that degrade the DNA are inactivated, protecting the DNA molecule.

"This method has a high DNA recovery rate and is suitable for extracting DNA from small samples."

"I thought it would be better to use this method to extract old blood stains, but it still failed."

"This method has also failed?"

Shiho Miyano thought for a moment and asked:

"How many STR loci were detected as a result?"

According to the DNA identification standard, all the sex loci and 15 STR loci were detected in the normosed body weight, a total of 16 positions, which was considered a success.

"The Chelex-100 method is much better than the salting out method, and 7 loci have been successfully detected."

"But it's not enough for DNA testing."

Lin Xinyi replied a little helplessly:

"The other appraisers didn't have anything to do, and I was the same, I just knew what to do, but I didn't know how to improve."

"So, I'm just going to come and ask you for advice."

"Miyano, do you have anything you can do?"

"It's simple." Shiho Miyano's calm tone was filled with lightness.

In fact, this knowledge is just the foundation of the foundation for a genius scientist like her, but being able to show it in front of Lin Xinyi can make her feel a rare pleasure.

Maybe it's because I helped Lin Xinyi.

Maybe it's because she can use her expertise to save people instead of killing people.

Shiho Miyano is very relaxed now, as if he has unconsciously stepped out of an invisible cage:

"Seven STR isotypes have been detected, indicating that the Chelex-100 method can indeed successfully extract DNA fragments from old blood stains, but the results are not satisfactory."

"In this case, there is no need for more advanced extraction methods, and a slight improvement of the Chelex-100 method may solve the problem."

As he spoke, Shiho Miyano pursed his lips lightly and thought deeply.

Although she had never studied DNA extraction for old blood stains before, she was extremely nimble and knowledgeable enough to quickly come up with an improved method:

"The old blood stains are tightly bound to the carrier, and the tangible components in the blood stains are naturally not easy to dissolve."

"So, if you use the conventional Chelex-100 method, the effect of DNA extraction will definitely be greatly discounted."

"We can try to soak the sample with ammonia to dissolve the nuclear material in the blood stains."

"Then increase the concentration of Chelex-100 appropriately to enhance its chelation force against metal ions and improve the extraction efficiency of DNA from old blood stains."

"If we improve it like this, we may be able to get better DNA results."

Miyano methodically proposes an improved method for extracting old blood stains.

"I figured it out so quickly..."

"You're amazing, Miyano."

Lin Shinichi said to Shiho Miyano very gratefully:

"Thank you, you helped me a lot!"

"It's just a matter of effort."

Shiho Miyano was not at all proud of the rudimentary knowledge he used.

For a scientist like her, who can overcome even the conservation of mass and energy, improving a DNA extraction method is probably a kindergarten-level difficulty.

Lin Xin put so much effort into asking this kind of question, it was really...

"Wait..."

"How did you unconsciously really talk about science..."

Shiho Miyano finally reacted.

Looking at the man in front of her who was thinking intently, her expression became a little subtle:

This guy...

You're really looking for me to study the figure?!