Zhang Lin took out the drum from the liquid nitrogen tank, then took out a cryopreservation tube from the cylinder, and then carefully put the cylinder back, because there were many other cell cryopreservation tubes in the cylinder. After Zhang Lin sealed the lock lid of the liquid nitrogen tank, he began a series of patient and meticulous operations.

He first put the cryopreservation tube in a water bath, quickly thaw it with hot water at 37 °C, then changed the centrifuge tube and centrifuge it with a centrifuge for five minutes, then sat down on the edge of the ultra-clean workbench, baked and disinfected the centrifuge tube nozzle to the alcohol lamp, discarded the waste liquid, added fresh culture liquid with a pipette gun, and repeatedly pipetted and mixed......

Gu Jun has been watching carefully from the side, and if he has any questions, he asked Senior Brother Zhang for advice, and he has learned a lot.

For example, the speed control of the centrifuge and the suction and discharge control of the pipette, the slightest sloppiness in these recovery operations will affect the survival rate and proliferation rate of the cells.

Finally, Zhang Lin injected the cell suspension into the Petri dish, added an appropriate amount of culture medium, and then put it into a cell culture incubator at 37°C and 5% CO2.

"Wait, at least two days." Zhang Lin took off his mask and pushed his glasses, "I suggest that you raise them for a week, so that the tumor formation rate can be guaranteed." ”

"If you raise it for two days, try a batch first." Gu Jun shrugged his shoulders and said, no way, there is not enough time, the system shows that his condition will worsen after 30 days, if it is true, it may be difficult to even get out of bed and walk by then. He said again: "Senior brother, go out and rub it at night?" I'm in for a treat. ”

"Excuse me." Zhang Lin waved his hand, "I'm going to feed the mice at night, I'm in for a treat." ”

……

For the next two days, Gu Jun spent the next two days on campus, in addition to gathering with friends, one is reading books and studying, preparing for the make-up exam, if he can't die but has to repeat the grade, it will be quite troublesome; The second is to go to the experimental building every day to take care of the cells and change the culture medium.

On the morning of the third day, Zhang Lin came to see it in person and said that the cells were barely growing, and he helped with subculture and the preparation of cell suspension.

And Gu Jun went to the school's laboratory animal center, which is actually a large breeding base, located a few hundred meters outside the school park, very lonely, probably because it is filled with the smell of various animals.

He bought five naked mice, each of which was more than 100 yuan, which was quite expensive, but fortunately he still had 20,000 yuan in savings, but it couldn't last long.

When the money is used, Gu Jun really regrets that he donated the money so early...... People are still alive, but the money is gone.

Ahem. Each of these nude mice is the size of two or three fingers together, without hair, and is packed in a sterile box, once they come into contact with the external bacterial environment, they can easily be infected and get sick and die. Therefore, the process of raising and experimenting needs to be carried out in a barrier environment.

The university's barrier environment laboratory is located at the Laboratory Animal Research Center, and the five-story yellow-brick building stands next to the laboratory building.

Gu Jun and Zhang Lin met in the lobby on the first floor of the research center and booked a small nude mouse room for one hour. This is a small room of only 21 square meters, divided into a front room and a back room, the front room has a laboratory table and instruments, and the back room is a breeding room.

Before entering, they and all the items they bring with them must be sterilized with strict ultraviolet light. The two of them used a special full-body protective suit, masks, gloves, foot covers and other full equipment to set foot in the nude mouse room.

Soon, on the anterior chamber test bench, Gu Jun marked five nude mice with a punching method, and then injected tumor cells subcutaneously on the right side of their buttocks one by one.

Zhang Lin is in charge of assisting on the side, and these cells can theoretically form tumors when they hit people. Now seeing that Tuhaojun is so aggressive with the naked mice, he is sure that this little brother is not trying to play murder.

After all five nude mice were injected, Gu Jun put them in the breeding cage, and then saw if they could form tumors.

"Senior brother, thank you." Gu Jun gave Senior Brother Zhang a thumbs up, without Senior Brother help, it would definitely not have gone so smoothly.

"The old Chinese medicine doctor you said ......" Zhang Lin still looked full of Conan's vigilance, "Isn't he really a liar?" ”

……

In the following week, Gu Jun continued his previous rhythm of life, but he went to the naked mouse room every day to see the cage of naked mice. Their rearing is taken care of by the management of the nude rat house, and each one costs 3 yuan per day for room and board.

He had only completed three ordinary tasks this week, because he couldn't do the ones who needed to treat the sick and save people, and even though the hospital was nearby, he wasn't a doctor yet. The tasks he completed were all related to medical exercises, and he already had 5 boxes of pills in his hand, and the proficiency of the Calm Hand was the first (3000/5000).

On the seventh day, Gu Jun once again walked into the nude mouse room in sterile equipment and observed the nude mice one by one.

None of them died, and a soybean-sized round tumor had grown under the skin on the right side of the buttocks of No. 1, which had been measured to be 0.5 cm in diameter, No. 3 and No. 4 also had obvious protrusions, 0.3 cm and 0.5 cm in diameter, respectively, and No. 2 and No. 5 still showed no signs of tumoricity.

At this point, he can be sure that out of five nude mice, three have tumors.

Gu Jun set two tumoritic nude mice as group A, one tumorigenic nude mouse as group B, and two non-tumoritic nude mice were divided into group C and group D, and went to the animal center to buy 10 healthy nude mice, 5 in group E and 5 in group F. Groups A, C, and E will all receive the same dose of "heterotextual medicine" (the name he gave it) every day; Groups B, D and F will not be administered at all, and will only be used for control purposes.

That is, tumoritic nude mice, non-tumorigenic nude mice and healthy nude mice, each have one group of drugs and one group of no drugs.

At this time, six cages had been divided into six cages on the experimental table, and the naked mice were chirping and making it lively.

Gu Jun was fiddling with 25 heterogeneous medicine capsules, since the system's instructions were that one box could extend life by 5 days, and a box of 5 capsules, it should be one pill a day. This is the dose for adult humans, and these nude mice weigh less than 20g on average, and if they also take one pill a day, it is estimated that they will be sacrificed immediately.

He poured out all the powder in a capsule, weighed the dose, and then converted the dose of the nude mouse with his own body weight, prepared a solution, and then diluted it proportionally.

Soon, Gu Jun held the gavage needle filled with the potion in one hand and a fixed healthy nude mouse in the other, and began the gavage administration of the first one.

"Chirp." The naked mouse struggled slightly, but this poor thing was still pushed by him to make its contribution to medicine.

At this moment, Gu Jun's heart was beating a little fast, and his eyes were staring at the naked mouse in his hand without blinking.

He had not been tested for the toxicity of a foreign drug until this point. If it dies suddenly, maybe the experiment will lose its meaning. And now, he stared at it for five minutes, then 10 minutes......

Half an hour later, the nude mouse still didn't show any abnormalities.

"It seems that there is no obvious acute toxicity of the heterotext." Gu Jun breathed a sigh of relief, "The chronic toxicity aspect still needs to be observed." ”

He then proceeded to dosing the next nude mouse. After ten minutes to complete the dosing of the other 7 nude mice in the three dosing groups, he observed them for another hour, and all of them had no abnormal reactions, and they were still able to scream, drink water and eat feed.

"Fellow rats, let's all live." Gu Jun muttered, pressed his aching head, his condition was obviously getting worse day by day, "Please." ”

……

On the second and third days, Gu Jun continued to come to the naked mouse room every day to work.

The tumors of the nude mice in group B without administration have increased from 0.3 cm to 0.6 cm, and their body weight and mental state have not changed, while the tumor size of the two nude mice in group A has not changed for two days, and it has remained at 0.5 cm.

Gu Jun knew what this might mean, heterotextual medicine really had a curative effect! These capsules can significantly inhibit the growth of tumors.

Moreover, the other nude mice administered were as usual, which also means that there seemed to be no adverse reactions to the drug and there was little damage to the body's functions.

"Maybe, this time it's really saved?" He thought to himself, and while the uneasiness still haunted him, a new hope burned at the same time.

Day 4 & 5...... A week has passed in the blink of an eye.

The tumor of the nude mouse in group B has grown to 1.5 cm, and the huge tumor is in stark contrast to its thin body, and it has begun to lose weight and become mentally poor. The two nude mice in group A continued to be in stable condition, and all the administered nude mice were alive and well, no different from the nude mice in the blank control group.

I have to say that the results of this experiment are very satisfying.

The efficacy of heterotextual drugs is better than the top tumor small molecule targeted drugs on the market.

However, Gu Jun also knows that the adverse reactions of foreign drugs cannot be completely ruled out, which only proves that it has no side effects in a short period of time. And after all, this is an animal experiment, which is different from human clinical experiments.

However, the results of this experiment were already worth the risk.

That afternoon, after Gu Jun wrote his suicide note, he came to the waiting area of the emergency department of the Affiliated Hospital of Dongda University and sat down in a rare waiting bench. It was crowded with patients and family members, mostly crying children and comforting parents, nurses hurriedly walking around, nurses pushing emergency beds......

There was a commotion, and the radio of the emergency department continued to call: "No. 182, Chen Ziyang." No. 183, Wang Yuxuan. No. 184, Huang Zixuan......"

Exhaled, Gu Jun resolutely threw a foreign medicine capsule into his mouth, drank a sip of mineral water and swallowed it.

"Come on." He looked at the emergency sign on the wall over there, "If there's any toxicity, come all." ”