Chapter 6 Ascites tumor-bearing mice

Cai Zixuan looked left and right for a moment before saying, "The leader of our group is Wang Ruoxiang. ”

"That's it?" Gu Jun frowned helplessly, thinking that something was wrong, "It's not surprising at all, it's not her, I'm strange." ”

"Yes, that's Wang Ruoxiang." Cai Zixuan sighed with emotion when he heard this.

Wang Ruoxiang is the leader of their class, with strong ability, beautiful personality and good personality. Such a goddess of learning, of course, the people who pursue her are from the teaching building to the bureau building, and Tuhaojun is one of them, who chased it vigorously back then, but finally ended with Wang Ruoxiang sternly refusing in public: "I'm sorry, we are not the same kind of people, I won't like people like you." ”

Well, a tall, handsome, rich, and even an eight-year man no matter how bad he is, is actually "this kind of person"?

At that time, everyone said that they were grateful to Tuhaojun and the squad leader for making them believe in love again.

"The squad leader is still single." Cai Zixuan obviously didn't know how bad he looked now.

"Don't look at me like that." Gu Jun laughed at himself, "It's all over." "It's really all over, Tuhaojun pursues the squad leader a little bit because he likes it, and a little bit because he wants to chase everyone's goddess, in the final analysis, it has nothing to do with those profound things.

"Yes." Cai Zixuan didn't continue to talk about the squad leader, and looked at his watch, "Hey, it's almost 8 o'clock, I have to go to the laboratory." ”

"Let's go, I'm going to the experimental building too." Gu Jun said.

Cai Zixuan suddenly showed a knowing look, just now he said that he didn't care about the class leader, and he was going to join in the fun, it's good to be young.

"What?" Gu Jun was really helpless, he wanted to explain but didn't know where to start, "Forget it......"

"Hao Jun, you don't need to say it, you understand, once the sea was difficult to water, I understand."

"You understand a sponge."

The medical laboratory building is in the northwest of the campus, which is a dozen stories of white-walled brick buildings with no features on the outside, and even opposite the entrance is a rotten concrete floor that has fallen into disrepair. However, there are well-equipped laboratories in the building for teachers and students to use for scientific research.

"This way." After entering the building, Cai Zixuan walked in front, introducing the situation to Gu Jun as he walked.

The topic of the experiment done by their group is called "Research on the Photodynamic Treatment of Tumors with Several Photosensitizers", which is a study in which tumors are implanted under the skin of mice, and then photodynamic therapy with different photosensitizers is used to observe the changes in tumors.

This time, the allocated funds are only 30,000 yuan, which is a lot for an undergraduate scientific research experiment, but an imported pure Photofrin photosensitizer can cost tens of thousands of yuan. In order to make several more photosensitizers, Wang Ruoxiang made various careful calculations, saved money and set up domestic imitation Photofrin group, dolteporfin group, ALA group, plus blank control group, a total of 5 groups of mice.

"The research plan and the proposal report were all done by the team leader." Cai Zixuan couldn't help but sigh when he said it, "It's really worthy of Wang Ruoxiang." ”

Although Professor Gu is a mentor, he will only occasionally find time to guide, and it is his business to rush for high-scoring SCI articles like his old man. Therefore, the Pioneer Cup still depends on the students themselves, and the group leader does the most, but it is an opportunity that everyone can't ask for.

Because if the experiment comes out with a paper, Wang Ruoxiang is the first author, and Cai Zixuan can only be ranked last.

"We are still in the first stage, which is to establish a mouse model of ascites tumor-bearing myocard, which has been cultivated for a week, and today I don't see it as a success."

Cai Zixuan said a lot, and suddenly found that Gu Jun didn't speak for a long time, and seemed to frown when he heard the word tumor......

"Uhhhh......h You know, modeling isn't 100% successful. ”

Cai Zixuan scratched the bald part of his head, he really didn't know what he just said, did Gu Jun understand.

"What is 100% in the world?" Gu Jun lowered his head and covered his face, friend, I haven't learned to be a scumbag yet, okay.

"Indeed." Cai Zixuan was very touched by this sentence, and his face swelled with vicissitudes, "Everything has a way, like a dream bubble." ”

Okay, Gu Jun doesn't understand......

During the conversation, the two took the elevator to the eighth floor, where the lab used by their group was located.

Walking along the corridor and outside the door of the laboratory, Cai Zixuan pushed open the door and walked in first, only to see that the team members had arrived, and he was the last one.

"Zixuan, I'm waiting for you."

"Huh?"

At this time, everyone saw a person who walked in later, and the laboratory suddenly fell silent, leaving only the chirping of mice.

...... local tyrants?

Didn't he drop out of school to travel the world? Didn't you say he hung up? It's not that he's ......

"Hello everyone." Gu Jun smiled and waved his hand to greet everyone, and looked at Wang Ruoxiang, "Hello squad leader." ”

Wang Ruoxiang stood by an experimental table, she had short and medium black hair, a delicate melon seed face that was very good-looking although she was plain makeup, and her long figure was wearing a white coat, and she was full of heroism. When Wang Ruoxiang saw him, she frowned slightly, "Hello Gu Jun." "The sound is also very good.

Gu Jun nodded in response to her, three months later, the squad leader is still so beautiful, but he is no longer the one he used to be.

"Hao Jun was suspended from school before, and now he's back." Cai Zixuan explained to everyone, and introduced Gu Jun again: Xu Hai, a classmate in clinical medicine, is an acquaintance, as well as Zhang Haoran, a major in basic medicine, and He Yuhan, a group of 5 people.

Cai Zixuan asked, "Squad leader, Hao Jun wants to take a look at us, it's not in the way, right?" ”

Wang Ruoxiang pursed her lips silently before saying, "Just stand aside and don't make trouble." ”

Tuhaojun. She muttered in her heart, remembering that at the beginning of school a few years ago, she had a superficial affection for him...... Vomit. This kind of person is lazy to eat, full of poisons, rotten wood cannot be carved, and mud cannot support the wall...... Mice are inferior! It may only be if someone pulls it to the slicing study that it will contribute to medicine.

"No, it won't." Gu Jun obediently stood aside, but he always felt that Wang Ruoxiang's eyes were not good...... Delusion.

On the experimental bench there is already a row of rat cages, each of which contains a group of white Kunming mice. This is the most commonly used experimental animal, very docile and small, 1-1.5 months old weighing only 18-22g, body length is less than 10cm, can be used for experiments.

Except for a cage of modeled mice, the others were all blank mice, which Wang Ruoxiang had just bought from the school's experimental animal center. Once the modeling is successful, they can come in handy.

Now that the team members have arrived, everyone immediately began to do things.

Crackling! Wang Ruoxiang put on rubber gloves and went to the mouse cage to catch mice, which were marked with numbers by dyeing, and she caught the No. 1 mouse. Cai Zixuan and several others were responsible for assisting, recording, recording and taking pictures.

"Intraperitoneal implantation of S180 mice fibrosarcoma on day eight." Wang Ruoxiang looked focused and serious, and gently pressed the No. 1 mouse's obviously bulging abdomen with her hand, and the mouse then let out a painful chirp, struggling, but she was firmly holding it in her hand.

She said: "The ascites of mouse No. 1 continues to grow, and it is already a frog's belly, and it has good vitality, so it does not need to be sacrificed for the time being. ”

Hearing what she said, the team members were suddenly a little excited, and they all leaned over to see it more clearly, "Is this the 'frog belly'?" "Awesome."

"It's worthy of a frog's belly, the belly is really big." Cai Zixuan said with emotion.

Although they are all eight-year programs, it is the first time that they have done such a real scientific research experiment.

"Oh." Gu Jun also leaned over to take a look, and he still understood what they were doing.

The reason why ascites tumor-bearing mice were first produced was to obtain a large number of highly active S180 tumor cells. You must know that the original cell line is very expensive, but one tube is only enough to inject one mouse, and the activity is very fragile, and the four treatment groups they set up need to culture hundreds of mice.

So it's about manufacturing first, and then expanding. Now the mice have grown frog bellies and can "collect ascites".

"Gu Jun." Wang Ruoxiang suddenly turned her head and glanced at him, "Can you walk away some?" It's in the way. ”

"Yes." Gu Jun shrugged his shoulders and walked away, your aura is okay, and you can get in the way of being a meter away from you.

Cai Zixuan didn't see it, Wang Ruoxiang continued to the next step.

She used a 5 ml syringe to puncture mouse No. 1's abdominal cavity, successfully extracted a full tube of yellow clear ascites, and injected it into an Eppendorf conical bottom centrifuge tube, which she then took to centrifuge with a centrifuge. Once centrifuged, you can immediately see a large amount of white precipitate at the bottom of the centrifuge tube, which is the tumor cells.

"Mouse No. 1 was successfully modeled." Wang Ruoxiang was relieved and happy, and smiled at the team members: "There are a large number of S180 tumor cells in ascites!" ”

"Yay!" "Haha." They were all young people, no one paid attention to the feelings of the mouse, they cheered, and Cai Zixuan praised: "Take the first step to success!" ”

The first step was successful, and then the cells were given to the treatment group to create subcutaneous tumors, which were then subjected to photodynamic therapy after tumorization.

There's still a lot to do, and they continue to be busy, removing the supernatant from the centrifuge tubes, resuspending the counts, and adjusting the cell concentrations......

To maintain cell viability, complete the full injection within an hour. So they're busy with one modeled mouse and then the next.

"Adjust the cell concentration to 1×10^7/ml......"

"Yuhan, you are responsible for taking 0.2 ml of cells per needle."

"Team leader, the anesthetic is ready!"

Each of them was so scrappy that no one cared about what happened to the local hero, and even forgot that he was standing aside and watching.

Gu Jun watched quietly, watching He Yuhan with cell suspension, Cai Zixuan anesthetized healthy mice with intraperitoneal injection of sodium pentobarbital, Xu Hai and Zhang Haoran marked the mice and removed hair on the buttocks, and finally Wang Ruoxiang injected tumor cells subcutaneously into the right buttocks of the mice......

No. 1, No. 2, No. 3, one after another.

Suddenly, Gu Jun's head cracked, was it a brainstem tumor......

The pain intensified violently, and he couldn't help but cry out in pain: "Ah! The scene before me became trance-like, flickering like the shadow of a nightmare.

He seemed to be unable to breathe air, as if he was in a ruined laboratory, with centrifuges, microscopes, sterilizers, and other instruments dumped on the floor, broken test tubes, messy documents, blurry picture frames, and rotten animal carcasses...... The dust turned it all into a gray color, and there was no breath of life, only chaos, dead silence.

The pain in his brain grew, but the vision became clearer, and he saw some indescribable instruments.

And the twisted corpses on the ground...... I couldn't tell what animal it was......

He saw that there was a line of blood on the wall inside, and it was the mysterious language on the pill box, but compared to the standard of printing, every stroke of that line of blood was terrifyingly weird.

In a trance, Gu Jun suddenly seemed to be able to understand the meaning of the blood word.

He muttered softly.